OBJECTIVE: To establish the method for the dissolution determination of Eslicarbazepine acetate tablets.METHODS: Samples were collected at 30 min with phosphate buffer solution(pH=4.5) as solvent and rotation speed of 50 r?min-1 according to second dissolution determination method(slurry method) stated in Chinese Pharmacopeia(2010 edition).The absorbance of 3 batches of samples was determined by UV spectrophotometry at detection wavelength of 288 nm.RESULTS: The method showed a good linear relationship in the range of 5～30 ?g?mL-1(r=0.999 8) with an average recovery of 99.74%(RSD=0.47%).The dissolutions of 3 batches of samples were 82.06%,82.72% and 82.64%,respectively.CONCLUSIONS: Established method is simple and reliable for the dissolution determination of Eslicarbazepine acetate tablets.

OBJECTIVEA fluorescence probe marked with a specific angle protein antibody by using quantum dots QDs605-CK (AE1/AE3) was utilized to detect metastasis and micrometastasis rates of early submandibular lymph node metastatic carcinoma in a nude mouse model. The method was compared with traditional immunohistochemistry (IHC) staining and hematine-eosin (HE) staining to explore a new method for early diagnosis and treatment of tongue squamous cell carcinoma.

METHODSHuman tongue Tca8113 cells were cultured and inoculated in 18 nude mice tongue body tissues (except the midline) to establish submandibular lymph node metastasis in tumor-bearing nude mice models. The nude mice were sacrificed, and the mandibular lymph node was dissected after injecting tumor cells for six weeks. One lymph node was divided into two parts: one was used to create a serial section embedded by paraffin then detected in HE staining and IHC staining; the other was immediately frozen in liquid nitrogen and detected using the fluorescence probe marked with a specific angle protein antibody by using quantum dots. Lymph node metastasis and micrometastases rates were calculated.

RESULTSThe rates of metastasis in the submandibular lymph node via quantum dot immunofluorescence staining, IHC staining, and HE staining showed positive values of 66.7%, 61.1%, and 27.8%, respectively. The micrometastases rates were 38.9% and 33.3% via quantum dot immunofluorescence staining and IHC staining, respectively. The positive rates of detecting lymph nodes metastasis with quantum dot labeled keratin CK (AEl/AE3) immunofluorescence and keratin CK (AE1/AE3) IHC staining were higher than that of conventional HE staining ('=6.379, P<0.05). The two methods showed no statistical difference (chi2 = 0.120, P > 0.05).

CONCLUSIONQuantum dots fluorescent probe marked with the angle protein antibody CK (AE1/ AE3) can precisely locate the submandibular lymph node metastasis tumor cell of the nude mice tongue squamous cell carcinoma, and the emitted red fluorescence showed strong specificity, high resolution, and a clear background. The method could be utilized to detect submandibular lymph node metastasis and micrometastasis of tongue squamous cell carcinoma in nude mice.

Animals ; Fluorescence ; Humans ; Immunohistochemistry ; Lymph Nodes ; Lymphatic Metastasis ; Mice ; Mice, Nude ; Neck ; Neoplasm Micrometastasis ; Quantum Dots ; Tongue ; Tongue Neoplasms

This article reviewed the theories and current researches of Ryodoraku Diagnostic System and pointed out its value in clinical studies, as well as the feasibility and significance of applying this system on the studies of electricity of meridians and collaterals.

Objective:To explore the changes of serum cholinesterase(ChE)and its clinical significance in patients with sepsis.Methods:Serum cholinesterase concentration,acute physiology and chronic health evaluation(APACHE)Ⅱ scores were estimated in patients.The patients in this study were divided into the sepsis group and the non-sepsis group.The patients with sepsis were further divided into the death group and the survival group.The influence of altered serum ChE levels on prognosis was compared between the groups.Results:(1)Serum ChE was significantly lower in patients of sepsis group than that of control group(P ＜ 0.01).(2)In patients with sepsis,the serum ChE level was significantly lower in the death group compared with that of survival group(P ＜ 0.05).(3)In patients with sepsis the serum ChE levels were significantly negatively correlated with APACHE Ⅱ scores(r=-0.280,P ＜ 0.05),positively correlated with serum albumin and body mass index(r=0.458 and 0.287,P ＜ 0.05).Conclusion:Serum ChE levels can be used to determine the prognosis and severity of illness in patients with sepsis.

OBJECTIVE:To establish the quality standard of Dibiling.METHODS:Rhizoma Coptidis in the formulation was identified qualitatively by TLC,and the content of ephedrine hydrochloride was determined by RP - HPLC.RESULTS: The TLC spots of Rhizoma Coptidis were clear and distinctive.The linear range of ephedrine hydrochloride was 0.088～3.520?g(r = 0.996 0) with an average recovery rate of 100.82%(RSD = 1.19%,n = 6).CONCLUSION:The established standard is applicable for the quality control of Dibiling.

OBJECTIVE: To prepare Flu mixture and establish its quality standard. METHODS: The methods of decoction- boiling and distillation were adopted to prepare the flu mixture; TLC was used to identify Radix Et Rhizoma Rhei and Rhizoma Curcumae Longae, and HPLC was used to determine the content of Artemisinin. RESULTS: The spots characteristic of Radix Et Rhizoma Rhei and Rhizoma Curcumae Longae. were clearly identified with TCL. A good linearity was seen of Artemisinin in the range of 0. 42～ 2. 10? g( r=0. 999 3) . The recovery rate was 99. 84% ( RSD=2. 15% ) . CONCLUSIONS: The preparation is simple in preparation technique and good in stability. The TLC method is highly exclusive. The HPLC method is simple, accurate, and can be used for the quality control of flu mixture.

OBJECTIVE To explore the risk factors of lower respiratory tract nosocomial infection in geriatrics patients after abdominal operation and the measures of prevention and control.METHODS The medical records of the 470 cases with abdominal operation from Jan 2003 to Sep 2006 were investigated retrospectively with prospective monitoring.RESULTS Among 470 cases,119 suffered from hospital infection,62 suffered from lower respiratory tract hospital infection,the infection rate being 13.19% and occupied 52.10% of the total number of the hospital infection in the abdominal operation.CONCLUSIONS Invasive operation,such as nasogastric tube,intubation,inhaling oxygen,sputum drawing out,cannulization,tracheotomy,and use of respirator after operation,lying in bed,vomit,using proton pump inhibitors,and underlying diseases are the causes of the lower respiratory tract hospital infection.To enhance the monitoring and control of the risk factors,we can decrease the rate of lower respiratory tract infection and mortality in geriatrics patients after abdominal operation.

Objective To investigate the biological characteritics of adult animal hepatocytes induced by phenobarbital sodium(PBS) in vivo,and to study the potential value of biological artificial liver of effective hepatocytes.Methods 12 adult male mice,are yandomly divided in to preinducing group and controll group.The preinducing group are intraperitoneally injected PBS per day,45mg/kg for 7 times in total;the controll ware injected NS.after that,we detected the blood serum TP,BUN,CHOL,HDLC.And the same amount of isolative hepatocytes was developed after being developed 48h;MTT was used to investigate the proliferation of hepatocytes after being developed 24h;chromosome was investigated to observe the cell division;and the survival deadline and morphology was also investigated.Results The TP had remarkable difference between the two groups(t=2.678,P

Objective To review the clinical experience of diagnosis and treatment of the congenital diaphragmatic hernia in newborn infants. Methods Thirty-three neonates were diagnosed having congenital diaphragmatic hernia in our hospital from Jan. 1,2004 to Sept. 30, 2009. The clinical data was retrospectively reviewed. Results 21 cases were treated surgically and 17 survived, while 4 cases died. The main cause of death was congenital pulmonary dysplasia. Another 12 cases refused to accept surgical treatment and they all died, one died shortly after he was born. Four cases who had been diagnosed by prenatal ultrasonography were survived. Conclusion The mortality of congenital diaphragmatic hernia in neonates was still high. Prenatal diagnosis of the congenital diaphragmatic hernia is very important and the cooperation between the obstetrics, neonatology and cardiothoracic surgery will improve the survival rate of congenital diaphragmatic hernia in newborn infants.

Objective To compare the pharmacokinetics difference of methotrexate ( MTX) alone or MTX combined with the decoction of Radix Salviae Miltiorrhizae ( RSM) in rats, and to investigate the possible impact of RSM on the efficacy and pharmacokinetics of MTX after oral administration. Methods Sprague-Dawley rats were given MTX (7 mg/kg) alone or MTX together with RSM (3.085 g/kg) respectively. At different time points, blood was sampled from orbital venous plexus and then was precipitated by perchloric acid. The serum concentration of MTX was determined by using high performance liquid chromatography ( HPLC) method. Chromatographic separation was achieved on a reversed-phase Diamonsil C18 (2) column with the mobile phase of methanol-formic acid water solution (formic acid in volume fraction of 0.1%) in gradient elution. The column temperature was 27 ℃, flow rate was 1 mL/min, detection wavelength was 302 nm, and the injection volume was 10 μL. The results were analyzed by pharmacokinetic software DAS ( 2.1.1) by non-compartment model. Results The linearity of the calibration curve for MTX was good in the range of 0.097 6 ~ 12.5 mg/L, the detection limit was 0.097 6 mg/L and the recovery was in the range of 77.5% ~ 86.6%. Compared with MTX group, the peak-arriving time of MTX-RSM group was advanced, and the clearance of MTX was delayed. Area under concentration-time curve at 0-t (AUC(0-t)) was increased by 0.609 times, and AUC(0-∞) was increased by 0.786 times. Conclusion The decoction of RSM exerts an effect on promoting the absorption of MTX, delaying the clearance of MTX and enhancing the bioavailability of MTX in vivo.