Objective To analyze the clinical value of sTNFR-1,sIL-2Ra and IGF-1 on depression in patients with esophageal cancer by comprehensive nursing intervention and explore the possibility of serum indicators to replace DSI and HAMD. Methods 120 patients with pathologically confirmed esophageal cancer were included in the study.Zung depression status inventory (DSI)and hamilton depression rating scale (HAMD)were used to assess depression.Serum sTNFR-1,sIL-2Ra and IGF-1were detected by enzyme-linked immunosorbent assay (ELISA).Results 69 patients were classified as anxiety and depression group,51 patients were as normal control group.There were 32 patients with depression.Serum sTNFR-1, sIL-2Ra and IGF-1 levels in study group was higher than that in the control group (P<0.05)and these serological indexs increased from the minimal depression,mild-to-moderate depression group to severe depression group (P<0.05).Conclusion Serum sTNFR-1,sIL-2Ra and IGF-1 levels may be used as evaluation index of nursing intervention,but need further clini-cal validation.

Objective To compare the masticatory efficiency of alveolar ridge restored by thermosetting resin full denture base and cast resin full denture base.Methods 30 patients with alveolar ridge were selected and divided intothe two groups by random number table and 15 cases in each group.Full denture with thermosetting resin base was worn in the first month in one group,and masticatory efficiency was measured.Then no false tooth was worn for a week,then full denture with cast resin base for a month,then masticatory efficiency was measured.In the other group,full denture with cast resin base was worn in the first month,and then masticatory efficiency was measured.Then no false tooth was worn for a week,then full denture with thermosetting resin base was worn for a month,then masticatory efficiency was measured.OD value as masticatory efficiency was measured by absorption spectrophotometry.Results The masticatory efficiency(OD value) of full denture with thermosetting resin base was (0.608 ± 0.124),and full denture with cast resin base was (0.885 ± 0.071).The masticatory efficiency of full denture with cast resin base was higher than that with thermosetting resin base (t =14.845,P ＜ 0.05).There was 13 cases of increased masticatory efficiency with hot coagulation resin base denture repair,the improved rate was 43.33 ％,while 24 cases with injection molding denture base denture repair,and the improved rate was 80.00％.The rate with injection molding denture base denture repair was higher than that with hot coagulation resin base denture repair(x2 =31.582,P ＜ 0.05).There was no significant adverse reaction in duration of test among all the patients.Conclusion The definition of full denture with cast resin base manufacture was high,and the masticatory efficiency of full denture with cast resin base is higher than that with thermosetting resin base,and the repair effects is better.It is worth of clinical application.

Objective To investigate the association between genetic polymorphisms of exon3 of nicotinic acetylcholine receptor ?4 subunit (CHRNA4) and sporadic Alzheimer's disease(SAD).Methods In 23 SAD patients and 30 people of normal control, exon3 of CHRNA4 was screened by PCR-DGGE and DNA sequencing.Results Three new genetic polymorphisms were found in the exon3 of CHRNA4: C104T, A136G, G169A.The frequnces of these three genes in SAD group were 35%,46%,61% respectively and in control group were 13%,13%,33% respectively. There were significant difference between two groups (all P

Objective:To prepare flupirtine maleate dry suspension and establish its quality control method. Methods: The dry suspension was prepared by a powder direct mixing method. With the sedimentation and redispersibility as the indices,the suspending effect of the hydrophilic polymers HPMC and CMC-Na was investigated. The optimal formula was obtained. The viscosity was deter-mined by a rotary viscometer. An HPLC fluorescence method was used to determine the content of main component. Results:The sedi-mentation ratio of flupirtine maleate dry suspension was 1 in 3h with good redispersibility and liquidity. The cumulative dissolution in 30min was above 80%. Conclusion:The quality of the prepared flupirtine maleate dry suspension is stable and controllable, and the production process is feasible, which provides basis for the research and development of new preparation of flupirtine maleate.

Objective To investigate the correspond of microsatellite instability (MSI) on the prognosis and the effect of chemotherapy for colorectal cancer.Methods The publications about the MSI of colorectal cancer from January 2011 to October 2012 were searched.The Web of Knowledge and MEDLINE (PubMed) databases were used for searching English articles.The CNKI and WanFang databases were used for searching Chinese articles.Statistical analysis and graphics were performed with Comprehensive Meta Analysis V2 software.Results Thirty papers were included in this study.For the prognostic Meta-analysis,MSI-H was a protective factor for the overall survival of patients (OR =0.634,0.558-0.720; P ＜ 0.001).Simultaneously,MSI-H was a protective factor for the disease-free survival of patients (OR =0.571,0.479-0.681; P ＜ 0.001).After using of 5-Fu,the survival rate of MSS/MSI-L patients could be significantly elevated (OR =0.515,0.433-0.611; P ＜ 0.001).However,the survival rate of MSI-H patients did not increase significantly (0.631,0.302-1.318; P =0.220).Conclusions Both the overall survival and disease-free survival of MSI-H patients were significantly better than that of MSS/MSI-L patients.For MSS/MSI-L patients,the chemotherapy effect was remarkable.However,for MSI-H patients,there was no statistics difference between chemotherapy and no-chemotherapy group.

Objective To evaluate the lung protective effect of sustained inflation(SI)and its mechanism for exogenous acute lung injury(ALI) animal models. Methods Exogenous acute lung injury animal models were replicated using intravenous injection of oleic acid. Immunohistochemis?try and double antibody sandwich ELISA were carried out to detect IL?10 and IL?6 protein levels in lung tissues and serum. Western blot was used to detect the expression level of SP?A in lung tissue and bronchoalveolar lavage fluid(BALF). The expression of ICAM?1 and SP?A mRNA in lung tis?sue was measured by reverse transcription PCR. The apoptosis of lung tissue cells was detected by flow cytometry. Results The maintenance of 20 s lung recruitment method of 35 cmH2O pressure could improve PaO2 and PaO2/FiO2,reduce PaCO2,and improve oxygenation and pulmonary com?pliance. The re?expansion effect of sustained inflation for exogenous acute lung injury was good. The IL?10 and IL?6 levels in lung tissue and serum for SI group were significantly higher than those of the control group(P<0.05). The SP?A expression level of lung tissue and BALF in SI group was significantly reduced compared with the control group(P<0.05). Flow cytometry analysis showed that the apoptosis rate of SI group was significant?ly higher than that of the control group(P<0.05). Conclusion Sustained inflation played an important role in lung protection against exogenous ALI through inhibition of apoptosis,lightening of inflammatory response,reduction of pulmonary edema and SP?A degradation in lung tissue,as well as promotion of SP?A synthesis and secretion in pulmonary alveolus.

Objective To investigate the effects of captopril on cardiac function and levels of energy-rich phosphates in pressure overload induced left ventricular hypertrophy rats. Methods One hundred and twenty SD rats were randomly divided into three groups: sham operation group (SH group, n=40),coarctation of abdominal aorta group (CAA group, n=40) and captopril treatment 1 mg· 1001·d-1) group (CAP group, n=40). Left ventricular end-diastolic pressure (LVEDP), left ventricular mass index (LVMI), levels of energy-rich phosphates and morphological changes of the myocardial mitochondria were compared at the 6th and 8th week after operation. Results At 6th week, in CAA group, LVMI and LVEDP were increased and ±dp/dtmax was decreased, while ATP and ADP were decreased and AMP was increased (P<0.01). These changes were much obvious at 8th week (P<0.01). Compared with those of CAA group, the parameters of heart function and energy-rich phosphates (ATP, ADP, AMP, TAN) in CAP group were improved significantly(P<0.01) at the 6th and 8th week. In CAP group, the parameters of heart function and energy-rich phosphates (ADP, AMP, TAN) were much better at 8th week than those at 6th week. The morphological change of mitochondria was less in CAP group than that in CAA group. Conclusion Captopril significantly improves myocardial energy metabolism in pressure overload rats and protects the function of myocardial mitochondria.

Objective To investigate the effect of dexmedetomidine on renal function in the patients undergoing cardiac valve replacement under cardiopulmonary bypass.Methods Fifty patients with rheumatic heart disease were randomly divided into the dexmedetomidine group (group D,n=25) and control group (group C,n=25).Arterial blood samples were collected before operation (T0),at postoperative 24 h (T1),48 h (T2),72 h (T3) and 96 h (T4) respectively.Serum Crea,blood urea nitrogen,cystatin C(Cys-C) and glomerular filtration rate(GFR) were detected by the Hitachi7600 automatic biochemical analyzer.Results Compared with T0,the levels of BUN and Cys-C at postoperative various time points were significant increased,while GFR was decreased,the differences were statistically significant (P<0.05).The levels of BUN and Cys-C started to gradually decrease and GFR started to increase from T2,the difference compared with that at T0 was statistically significant(P<0.05).But the levels of various indicators at T4 did not recovered to those before operation;compared with T0,serum Crea level had no statistical difference among postoperative various time points(P>0.05).Serum Crea level at T2-T4 in the group D was significantly lower than that at T0,the difference was statistically significant (P<0.05).There was no statistically significant difference in Crea,BUN,Cys-C and GFR between the two groups(P>0.05).Conclusion Dexmedetomidine can promote the recovery of postoperative renal function in the patients with cardiac valve replacement under cardiopulmonary bypass and has a certain protective effect on kidney.

OBJECTIVE: To observe waveform differences among ocular vestibular evoked myogenic potentials (ACS-oVEMP) elicited by different types of air conducted sound in normal young Chinese subjects. METHOD: Twenty-two adult volunteers (44 ears) were recruited as research subjects including 12 males and 10 females aged between 18 and 35. Five hundred Hz tone burst, 125 Hz tone burst and click were employed as stimulus for conventional ACS-oVEMP examinations in bilateral ears of each subject. The response rate, threshold, n I latency, p I latency, n I-p I latency interval, amplitude and inter-aural asymmetry were recorded and compared among groups. RESULT: The response rate was 100% (44/44) in 500 Hz tone burst group, 40.9% (18/44) in 125 Hz tone burst group and 29.6% (12/44) in click group There were statistical significant differences between the 500 Hz tone burst group and the other groups (P < 0.05). Waveform amplitude was the largest in the 500 Hz tone burst group (7.3 +/- 5.5) muV than that in the 125 Hz tone burst group (3.8 +/- 1.5) muV and that in the click group (5.8 +/- 1.3) muV, which had statistically significant differences between the former and the later two groups (P < 0.05). Other waveform parameters presented similar differeces. CONCLUSION ACS-oVEMP waveforms could be largely affected by different stimulus. 500 Hz tone burst was the best stimulus with highest response rate, biggest amplitude and therefore most easily recognizable waveform in ACS-oVEMP examination.
Acoustic Stimulation ; Adolescent ; Adult ; Asian Continental Ancestry Group ; Female ; Humans ; Male ; Vestibular Evoked Myogenic Potentials ; Young Adult

BACKGROUND:Acel ular tracheal matrix is similar to the native trachea with structure and biological performance preserved after decel ularization, and it is an important aim in tissue engineering to find an effective method of decel ularization.
OBJECTIVE:To select the optimal decel ularization method through comparing chemical extraction method with detergent-enzymatic method for preparing rabbit tissue engineering trachea matrix.
METHODS:Thirty tracheas from New Zealand white rabbits were randomly divide into three groups. Twenty of rabbit tracheas were subjected to decel ularization using 2%TritonX-100 combined with deoxyribonuclease I and ribonuclease (chemical extraction method group), and sodium deoxycholate combined with deoxyribonuclease I (detergent-enzymatic group), respectively. The other ten were given no intervention as controls. Samples were col ected and observed by hematoxylin-eosin staining, Masson-trichrome staining, safranin O staining, DAPI staining and scanning electronic microscope.
RESULTS AND CONCLUSION:Hematoxylin-eosin staining demonstrated that compared with the control group, almost al cel ular components of the mucosal epithelium were removed in the detergent-enzymatic and chemical extraction groups, and there were few remnant chondrocytes. Masson-trichrome staining indicated that compared with the control group, components of the mucosal layer chondrocytes in the chemical extraction and detergent-enzymatic groups were completely removed, with only part of remained chondrocytes in the cartilage zone. Glycosaminoglycan was slightly decreased both in the chemical extraction and detergent-enzymatic groups shown by Safranin O staining, but more reduction was found in the chemical extraction group. DAPI staining reveled that only a smal amount of cartilage cel s remained in the dense layer of cartilage and lacuna both in this two methods. Scanning electronic microscope showed that using the detergent-enzymatic method there were the hierarchical structures of native trachea, but slight disruption using the chemical extraction method. In conclusion, decel ularized rabbit trachea matrix obtained by detergent-enzymatic method is better, with little disruption to the matrix.