OBJECTIVE: To investigate the effects of aldosterone (Ald) on the proliferation and collagen synthesis of cardiac fibroblasts (CFs) in neonate rats and to research its mechanism. METHODS: The neonatal rat cardiac fibroblasts were induced by anchorage velocity-dependent separation method. SD rats were divided into control group, Ald (1.0?10-7 mol?L-1) group, Ald combined with spirolactone group and Ald combined with losartan group. After 24 h of exposure, CFs proliferation was measured by MTT assay and cell cycle distribution was determined with flow cytometer (FCM). Spectrophotography was used to detect the content of hydroxyproline and immunofluorescence staining method was applied to determine the protein expression of proliferation cell nuclear antigen (PCNA) and fibronectin (FN). RESULTS: As compared with control group, in Ald group the activity of CFs was increased as well as its percentage in S stage. The content of hydroxyproline and the protein expression of PCNA and FN were also increased while the percentage of CFs in G0/G1 stage was decreased (P0.05). CONCLUSION: Ald-stimulated CFs proliferation and collagen synthesis were mediated by activation of AT1 receptors and Ald receptor,and Ald receptor plays a major role. A corresponding increase in the protein expression of PCNA and FN in CFs may be related to the proliferation effect.

level and recommended nutrient intake)in China has been out-of-date.Therefore,it has became an urgent problem for Chinese Nutrition Society to re-evaluate the DRIs of vitamin D and make corresponding modifications.

The article searches for the methods which combine teaching with scientific research in functional experiment in order to cultivante the students' ability of innovation and practice.

Objective To clone and construct eukaryotic expressing vector of bcr-abl fusion gene and to express the gene in the mammal COS-7 cell lines. Methods bcr-abl fusion gene was amplified from human chronic myeloid leukemia (CML) K562 cell lines by RT-PCR and the fragment of cDNA was retrieved,purified and cloned into the pEGFP-N3 eukaryotic expressing vector. After the selection of the positive clone and by restriction enzyme analysis and DNA sequencing, the correct plasmid was transfected into COS-7 cell lines and observed the transient expression. Results A 874 bp DNA fragment was amplified by RT-PCR. The sequence analysis showed it was consistent with bcr-abl gene of GeneBank. RT-PCR, Western blotting analysis provided strong evidences that bcr-abl gene was expressed successfully in transfected COS-7 cells.Conclusion The eukaryotic expressing vector of bcr-abl fusion gene was constructed, it will lay the foundation for further study of bcr-abl gene in the diagnosis and treatment of CML.

Objective:To discuss the use and management of medical material in hospital in order to resolve the key problem during medical material was used in department of device management.Methods: Using the modern logistics management idea to overall upgrade the original system. And to reconstruct the hospital integrated operations management platform included all aspects, such as design ideas, technical architecture, software function and so on, from whole management requirement point.Results: This platform could provide the improvement solution of traceability management for medical material in hospital, and increase the efficiency and transparency of management, and improve the whole management level in hospital.Conclusion: The introduced integrated operations management platform has standardized high-value materials management, met the fine management and cost requirements, and greatly improved the management level of medical materials.

Objective To study the identification method of Akebia trifoliata(Thunb.) Koidz. by Fourier Transform Infrared (FTIR) Spectroscopy. Methods FTIR Spectroscopy was measured of Akebia trifoliate collected from different production areas. Results At the range of 737-1032cm-1, the Spectroscopy of Akebia trifolia of different production areas showed variances in peak value of infrared absorption, peak position, peak shape and peak strength, which can be regarded as identification evidence for Akebia trifoliate. Conclusion This mehthod is rapid, reliable, simple and effective. FTIR can be used as the identification index for Akebia trifoliate.

Objective To investigate the changes of the serum level of IL-6 and IL-10 as well as their significance after coronary artery stent implantation. Methods The study involved 30 patients who underwent coronary artery stent implantation. Blood stamples were taken from the femoral arteries before and 24h after successful coronary stenting. Serum concentration of interleukin-6 (IL-6) and interleukin-10 (IL-10) were measured and complications as well as the occurance of restenosis were observed after the procedure. Results The concentration of IL-6 in femoral aterial blood was significantly higher after 24 hours of stent-implantation (214.6?118.0 ng/L vs 175.8?81.8 ng/L, P 0.05, and the ratio of IL-6/IL-10 increased (3.2?1.9 vs 2.3?1.0, P

Hypoxia-inducible factor-1 (HIF-1), as a nuclear transcription regulator of the hypoxic response, is up-regulated during hypoxia, and it regulates a series of downstream target gene expression, such as vascular endothelial growth factor, glucose transporter and erythropoietin through binding with hypoxia response element. It plays important roles in angiogenesis, anerobic metabolism, cell survival, proliferation, migration, and differentiation.This article reviews the structure, function and activity regulation of HIF-1 and its roles in acute ischemic brain injury.

Objective To study the effect of paeonol (PAE) and panax notoginseny saponins (PNS) on the expressions of collagenⅠandⅢprotein and mRNA in rats with acute myocardial infarction (AMI), and to explore the molecular mecha?nism of improving myocardial fibrosis. Methods The rat model of AMI was made using the left anterior descending coro?nary branch ligation.According to the intervention rats were divided into model group, PAE (8 mg/kg) group, PNS (40 mg/kg) group, PAE (4 mg/kg)+PNS (20 mg/kg) group, PAE (8 mg/kg)+PNS (40 mg/kg) group and captopril positive control group (10 mg/kg). Sham operation group, only wear line without ligation. The left ventricular mass index (LVMI) was detected after treatment for 28 d. Masson staining was used to observe changes of myocardial fibrosis. Western blot assay and RT-PCR technique were used to detect protein and mRNA expression levels of collagenⅠandⅢ. Results The values of LVMI were increased in model group compared with those of sham operation group and treatment groups. Compared with PAE group and PNS group, values of LVMI were significantly decreased in PAE (4 mg/kg)+PNS (20 mg/kg) group and PAE (8 mg/kg)+PNS (40 mg/kg) group. There was a more significant decrease in formula high dose group (P < 0.01). The model group showed pathological change. There were different degrees of improvement in pathological structure in all treatment groups, more sig?nificant improvement was found in formula low dose group, formula high dose group and captopril positive control group. There were different degrees of increase in expressions of collagenⅠandⅢprotein and mRNA in model group compared with those of sham operation group and treatment groups. Compared with PAE group and PNS group, the protein and mRNA expression levels of collagenⅠandⅢwere significantly decreased in formula low dose group and formula high dose group,more significant decreased was found in formula high dose group (P<0.05). Conclusion Compound of paeonol and PNS can improve myocardial fibrosis in myocardial infarction rats, which may be related with reduced expressions of collagenⅠandⅢprotein and mRNA.