AIM To establish the method for quality control of Qiwei Ketenzi Pill (Semen Entadae, Semen Nigellae, Fructus Piperis, ect). METHODS Semen Nigellae was identified by TLC, and vitexicarpin was determined by HPLC. RESULTS TLC spots developed were fairly clear, and the bland test showed no interference. vitexicarpin in Qiwei Ketenzi Pill showed a good linear relation in the concentration range of 0.106 5 - 2.130 0 ?g and the average recovery was up to 101.4 % , RSD was 1.34 T《B》 % . CONCLUSION The method is effecive for the quality control of Qiwei Ketenzi Pills.

AIM: To study the expression and distribution of osteopontin (OPN) in lungs and pulmonary arteries in pulmonary hypertensive rats induced by hypoxia-hypercapnia, and to explore the role of OPN in pathogenesis of pulmonary hypertension. METHODS: Forty-eight male Sprague-Dawley rats (Weight 180 g-220 g) were randomly divided into four groups: normal control group (NC), hypoxic hypercapnia 1-week,2-week and 4-week group (1HH, 2HH and 4HH). The expressions of OPN mRNA and protein in lungs and pulmonary arteries were detected by RT-PCR and immunohistochemistry. ELISA was used to detect the concentration of OPN in lung homogenates. The content of OPN in pulmonary arteries was detected by Western blotting. RESULTS: ① The mean pulmonary artery pressure (mPAP) and weight ratio of right ventricle to left ventricle and septum [RV/(LV+S)] in all hypoxic hypercapniac groups were higher than those in normal control group (P0.05). ② The expression of OPN mRNA was significantly increased in pulmonary arteries and lung tissues in hypoxic hypercapnic groups compared with normal control group (P