In recent years,food intolerance has gradually become the focus of public attention.Food intolerance is a complicated allergic disease,which is different from food allergy.It may lead to systemic diseases.Because of its hidden and no specific symptoms,the diagnosis is often difficult.Through the detection of food specific IgG antibody,no intake or less intolerance food intake,can effectively improve the patient's symptoms,control and prevention of disease.This article reviews food intolerance from six aspects including its pathogenesis,the difference of food allergy,morbidity,the relationship with pediatric headache,the evaluation of diagnostic technique and nutrient adjustment.

Health insurance is an important part of social security, and many problems have arisen in the con-struction of its legal system, such as legislative lag, lack of coordination among law enforcement, and serious disloca-tion of judicial relief. Therefore, under the vital time of performing the strategy of “rule by law”, the development and reform of the legal system for health insurance in China should be promoted. On the basis of summarizing the his-torical development of the legal system for health insurance in China, this paper proposes solutions such as centrali-zing legislative upgrades and expediting the process of unitary and special laws from the perspective of“rule by law”. The system of personal credit should be established and judicial transparency should be promoted to improve the legal system for health insurance.

Background and purpose:ERp29 belongs to endoplasmic reticulum stress proteins and might play roles in neoplasm metastasis. This study aimed to investigate the expression of ERp29 in Hca-F and Hca-P cells and to elucidate its role in lymphatic metastasis.Methods:Immunohistochemistry, Western blot and lfow cytometry analysis were used to detect the expression of ERp29 in Hca-F and Hca-P.Results:The results of immunohistochemistry suggested that ERp29 protein was located at cytoplasm of hepatic cells and some were also detected in the nucleus. The results of western blot suggested that ERp29 had positive expression in Hca-F and Hca-P cells. Its expression in Hca-F cells was apparently lower than that in Hca-P cells. And there was statically different between Hca-F and Hca-P cells (P<0.01). The relative lfuorescence intensity of ERp29 protein was signiifcantly lower in Hca-F cells (375.27±47.33) than that in Hca-F cells (623.91±46.80) by lfow cytometry (P<0.01).Conclusion:The different expression of ERp29 may be related to the potential ability of tumor lymphatic metastasis in Hca-F and Hca-P cells.

Cardiovascular disease in peri-menopausal period is a kind of climacteric syndrome which based on the symptoms of the cardiovascular system as the main manifestations. It is manifested as hypertension,pseudo-angina,arrhythmia. Hormone replacement therapy or symptomatic treatment is the common used method of modern medicine,although a certain degree of efficacy,but there are also significant disadvantages. In the treatment of cardiovascular disease in peri-menopausal period,TCM has certain advantages. In this paper,we treat cardiovascular disease in peri-menopausal period from the theory of 'yin deficiency,blood stasis,heat-toxin'and propose the method of 'nourishing yin,promoting blood circulation and detoxification'.

Objective Culture,expansion and characterization of pseudomyxoma peritonei cells to support foundation for the study of pseudomyxoma peritonei and drug screening.Methods Tumors from 5 cases of human pseudomyxoma peritonei were cultured by collagenase digestion.The cultured cells were then identified as tumor cells by chromosome karyotyping.The secretion of neutral mucopolysaccharide was detected by PAS staining.To simulate the tumor growth condition in vivo,3D culture was applied and the morphology of the cultured organoid was observed with HE staining,xenografts were used to test the tumorigenicity of PMP primary cells.Results The primary culture of pseudomyxoma peritonei cells was successful in all of the 5 cases.2D cultured cells were adherent,polygonal pebble-like arranged,and passed on up to 18 generations.Chromosome karyotype analysis showed that most of the cells were subdiploid karyotype tumor cells.Positive PAS staining suggests that these cells secrete mucus.The morphology of 3D cultured organoid was similar to that of tumor tissues.The tumor formation rate of xenografts was low,and the tumoris not similar to patient's PMP.Conclusions Pseudomyxoma peritonei tumor cells can be cultured and expanded in vitro.

Purpose　The aim is to develop a new method for the determination of the activity of hepatocyte growth factor (HGF). Met hods　 Rat hepatocytes were prep ared by collagenase perfusion and incubated at 5% CO2 incubator for 1.5 h. The n HGF was added into the culture medium and incubated at 5% CO2 incubator for 1.5 h. The cell viability was determined by MTT method.Results　～1 142 μg/ml improved the number of cell viability significantly （AHGF/Acontrol） ＞2.Conclusion　Th is new method was stable, time-saving and of good reappearance. It was adapted to the determination of HGF activity.

Objective To investigate the effects of dexmedetomidine (DEX) on oxidative stress and inflammatory cytokines caused by tourniquet-induced ischemia-reperfusion injury at limbs.Methods Eighty patients who had been scheduled for lower limb operation with tourniquet were assigned equally by sequence number to use or not use DEX (DEX or control group,n =40).Combined spinal-epidural anesthesia was performed in both groups.In the DEX group,DEX intravenous infusion was started immediately after the femoral vein cannulated at a dose of 1 μg/kg for 10 minutes,followed by 0.5 μg/kg · h until the end of operation,whereas the control group received an equivalent volume of 0.9％ saline.At 10 min before tourniquet inflation (T1),10 min (T2),30 min (T3) and 60 min (T4) after tourniquet release,femoral venous blood samples were obtained to measure heart rate (HR),mean arterial pressure (MAP),saturation of pulse oximetry (SPO2),serum malondialdehyde (MDA),serum superoxide dismutase (SOD),serum tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) levels in both groups.Results There were no significant differences in HR,MAP or SPO2 at all time points between the 2 groups (P ＞ 0.05).There were no significant differences in HR,MAP or SPO2 at all time points within either group (P ＞ 0.05).There were no significant differences in serum MDA,SOD,TNF-α and IL-8 levels at T1 between the 2 groups (P＞ 0.05).The serum MDA,TNF-α and IL-8 levels were significantly lower and the serum SOD level significantly higher in the DEX group than in the control group at T2,T3 and T4,respectively (P ＜0.05).In both groups,the serum MDA,TNF-α and IL-8 levels were significantly higher and the serum SOD level significantly lower at T2,T3 and T4 than at T1,respectively (P ＜ 0.05).Conclusion Dexmedetomidine can reduce the oxidative stress and inflammatory cytokine level which are caused by tourniquet-induced ischemia-reperfusion injury.

Objective To observe effects of electro-acupuncture on the expression of calcitonin gene-related peptide (CGRP) and substance P (SP) in constipation-predominant IBS rats,and to discuss the mechanism of IBS's acupuncture treatment.Methods To set WISTAR adult rats as the object and to establish models of irritable bowel syndrome rats.The experiment rats were randomly divided into a normal group,a model group,a EA Shangjuxu group and a EA Dachangshu group.The Shangjuxu group and Dachangshu group were bounded and given EA,once daily,for a total of seven times.Model group was bounded but not treatedwith EA,the normal group was done nothing.After the treatment,5ml of inferior vena blood was got inanesthesia state.The value of plasma CGRP and SP in rats' plasma were measued with Elisa.Results Compared with the normal control group,CGRP and SP content[(52.62± 15.05)pg/ml,(27.10±5.03) pg/ml] in IBS model group was obviously increased,the difference was statistically significant (P＜ 0.01,P＜ 0.01) ; the changes of plasma CGRP content [(37.82± 15.34)pg/ml,(38.93± 12.05)pg/ml] and SP content[(19.62± 5.68) pg/ml,(18.90± 5.07)pg/ml] were not obvious in EA Shangjuxu group and EA Dachangshu group (P＞0.05,P＞0.05).Compared with IBS model group,the content of plasma CGRP and SP was significantly descend in EA Shangjuxu group and EA Dachangshu group (P＜0.05,P＜0.05,P＜0.01,P＜0.01).The concentration of CGRP and SP in EA Shangjuxu group and EA Dachangshu group had no significant difference(P＞0.05,P＞0.05).Conclusion Electroacupuncture could treat IBS through regulating CGRP and SP in IBS rats.

Objective To investigate the clinical and computed tomography (CT) features of lung isolated ground-glass opaci-ty and to analyze the predictive factors that may discriminate benign from malignant nodules .Methods Clinical features , CT charac-teristics, pathological diagnosis , and the follow-up results were retrospectively analyzed with 108 cases of isolated ground-grass opacity (GGO) that was confirmed by histopathology from January 2011 to March 2013.Results A total of 108 isolated GGOs [52 men,56 women,mean age (58.12 ±10.34)years] included 19 benign nodules and 89 malignant nodules.The diversity of age, gender, clini-cal symptom, margin (speculation, spine-like process), internal characteristics (ovule sign, air bronchograms) of the lesions were no statistically significant differences between benign and malignant GGOs ( P >0.05 ) .The lymphocyte and CT findings of shape , lobu-lation, interface (ill/well defined) and pleural indentation sign were significantly different between benign and malignant GGOs ( P <0.05 ) .Besides significant difference in pleural indentation sign was also found between bronchioalveolar carcinoma and adenocarcino -ma ( P =0.0252).Among the 44 patients who were followed up after finding GGO initially , there were 7 GGOs (7/44,15.91%) to grow up after 3years, and GGOs of 32 patients had no obvious change , besides the patients'age of malignant GGO was bigger than the benign( P =0.0416).Conclusions Clinical features were of little value to discriminate the benign from malignant GGOs .CT fea-tures including round/oval shape, lobulation, and well defined and pleural indentation sign were of important value in the diagnosis of malignant GGO .Surgical resection should be applied in the process of follow-up when the sizes of GGO become bigger or newly formed solid components were detected .GGO that can't be diagnosed should be followed up for at least 3 years.

Objective To investigate the relationship between primary TCM syndromes and heart rate variability in hypertension, evaluate the development of disease. Methods 182 hypertension patients were recruited in accordance with the standard of hypertension. According to the Chinese medicine dialectical criteria, these patients were further divided into 5 groups, namely, hyperactivity of liver-fire group, phlegm-dampness retention group, blood stasis group, hyperactivity of Yang due to yin deficiency group and Qi and blood deficiency group. Another 60 healthy people were recruited as a control group. 24h Holter was adopted to monitor heart rate variability. Results Analysis of TCM syndromes and heart rate variability, anger on inflammation group had positive relation with SDNN, RMSSD, LF, HF and TP(r were respectively 0.161,0.372, 0.223, 0.281, 0.301);Phlegmy wet resistance groups had positive relation with SDNN, HRV triangular index, VLF, LF, VLF/HF, LF/HF, LFnorm.(r were respectively 0.175, 0.211, 0.174, 0.262, 0.159, 0.264, 0.263);blood stasis group had negative relation with LF/HF, LFnorm(r were respectively-0.185、-0.018).Yang due to yin deficiency group had negative relation with SDNN, RMSSD, VLF, LF.(r were respectively -0.204, -0.161,-0.184,-0.197);Qi and blood deficiency group had negative relation with SDNN, SDANN, RMSSD and HRV triangular index, VLF, LF, LFnorm, and had positive relation with HFnorm(r were respectively-0.353,-0.348,-0.258, -0.303, -0.268, -0.361, -0.352, 0.267). Conclusion Hypertension patients with different type of TCM syndromes had different characteristics of autonomic nerve functional disorders, which both reflected the important pathological changes of TCM syndromes and had close relation with the course of hypertension, providing objective basis for clinical diagnosis of syndrome differentiation.