BACKGROUND:Several studies have demonstrated that the physiological process of the ovary, including folicle development, maturity, ovulation, corpus luteum formation and regression, is related to the formation of extracelular matrix. However, matrix metaloproteinase 9 and connective tissue growth factors had a close relationship with the formation of extracelular matrix. OBJECTIVE:To investigate the relationship between the expression of connective tissue growth factor and matrix metaloproteinase 9 and polycystic ovary syndrome. METHODS:The female SD rats with regular estrous cycle were randomly divided into control and model groups. The rats in the model group were intragastricaly with letrozole to establish the model of polycystic ovary syndrome, while the rats in the control group were intragastricaly with the same amount of carboxymethyl celulose. When the natural law of estrous cycle of rats in the model group did not exist and consecutive interval appeared, rat serum and ovarian tissues were colected. Rats in the control group were detected by colecting specimen in the interval. RESULTS AND CONCLUSION:Radioimmunoassay and immunohistochemical staining results showed that compared with the control group, the expression of serum luteinizing hormone, serum prolactin levels, connective tissue growth factor in preantral folicles theca cytoplasm, connective tissue growth factors in folicular basement membrane cytoplasm, connective tissue growth factors in albuginea and matrix metaloproteinase 9 in corpus luteum significantly increased (P < 0.05). The contents of folicle stimulating hormone and serum estradiol, and expression of matrix metaloproteinase 9 in folicular basement membrane significantly decreased (P < 0.05). The results indicate that connective tissue growth factors may be related to the excessive growth of smal folicles and ovulation disorders in polycystic ovary syndrome, while matrix metaloproteinase 9 may be related to ovulation disorders in polycystic ovary syndrome.

The serial knowledge of cognate thoughts, culture, systems, language, engraving and edition of classic ancient books was organized based on their subject-guided reading activities, which plays a rather effective role in promoting the readers to read them. The selection of subject-guided reading activity contents, forms of subject-guided reading activity design, implementation of subject-guided reading activity were analyzed with the ancient e-dition of《Handbook of prescriptions for emergency》as an example. A knowledge chain-based subject-guided reading model of classic ancient books was proposed for promoting the reading of ancient books on traditional Chinese medi-cine and the reading of Chinese national culture in related academic libraries.

Caveolin-1 is an important surface structural marker of Caveolae. It is closely involved in the proliferation, apoptosis, invasion, metastasis, angiogenesis, signal transduction and multi-drug resistance of various tumor cells. Recent studies have found that altered Caveolin-1 expression has dual roles, as a tumor suppressor and promoter, in different stages of gastrointestinal cancer development and progression. The exact mechanisms of Caveolin-1s' involvement in the development of gastrointestinal cancer remain to be clarified and have attracted the attention of a lot of researchers.

Objective To explore the effect of Mudan granule combined with zinc sulfate on ankle brachial index in the treatment of diabetic foot dorsal artery sclerosis and its efficacy.Methods 66 cases of patients with diabetic foot dorsal artery sclerosis in this study were selected and divided into 2 group, 33 cases in each group.Control group received mecobalamin injection and zinc sulfate tablet and treatment group received Mudan granule on the basis of control gorup.7 days for a course of treatment and patients received continuous treatment of 4 courses.Clinical efficacy, blood glucose, blood lipids, blood rheology, blood flow dynamics of the dorsal artery of the foot, ankle brachial index, and adverse reactions were compared after the treatment.Results Compared with the control group after the treatment, the total effective rate of the treatment group was higher(P<0.05).Compared with the control group, the serum postprandial 2h blood glucose, total cholesterol, triglyceride, low density lipoprotein cholesterol levels were lower(P<0.05), and high density lipoprotein were higher(P <0.05), the levels of whole blood viscosity high, middle and low shear, plasma viscosity, hematokrit and fibrinogen were lower(P<0.05), the serum levels of appeal index were lower(P<0.05), the blood flow of the dorsal artery, the diameter of the blood vessels and the ankle brachial index were higher (P<0.05), the peak velocity of the dorsal foot artery was lower(P<0.05) .There was no significant difference in the incidence of adverse reactions between the 2 groups.Conclusion Mudan granule combined with zinc sulfate tablet in the treatment of diabetic foot dorsal artery sclerosis was effective with high safety and it could improve ankle brachial index.

Circular RNA ( circRNA) , a novel type of endogenous non-coding RNA ( ncRNA) , has been broadly taken into account during recent years as a research hot-spot.Unlike linear RNA, circRNA form covalently closed loop structure by jointing 3′and 5′ends together via exon circularization or intron circularization.Through which way it is more stable and conserved than linear RNA.Besides, circRNAs are abundant and multiple existences in creatures have been revealed.Nowadays, an increasing number of researchers have found that circRNA plays an essential role in the regulation of gene expression, which has broadened the view of di-verse endogenous noncoding RNA, and therefore hints that circRNA has great potential in clinical diagnosis and treatment of disease. In this paper, we make an overview of the latest research about circRNAs from the aspects of generation, character, function, and the relationship with diseases, and so on.

Capsular warning syndrome (CWS) is a subtype of recurrent stereotyped transient ischemic attacks that heralds a subsequent stroke.The mainly presumed mechanism is repeated bursts of ischemia in the region of penetrating arteries,since the structural changes of large arteries is rare in series of imaging examinations.The majority of the infarct is located in the internal capsule or pontine.CWS is usually therapeutically resistant to simple traditional antithrombotic treatment,while combined antiplatelet treatment,thrombolysis,endovascular intervention and antiepileptics may protect part of the patients from developing into permanent infarcts.

Objective To summarize the treatment experience of extensive burn merging smoke inhalation acute lung injury(SIALI)in 32 early stage cases,and to provide a reference for the further improvement of success rate in early stage. Methods A total of 32 cases of extensive burn merging SIALI resulting from mine explosion that received clinical treatment and triage from November to December in 2014 were enrolled for the study. In addition,the experience of triage and prognosis(after injured in a month)to guide therapy was analyzed. Results Two of the 32 patients died from respiratory failure in shock period,after shock period(3 days after injured),19 bad sufferers were cured in the local hospital,and the oth?er 11 cases in stable condition were triaged to the nearby hospital. 1 month follow?up results showed that:patients who received therapy in local hospi?tal,one died from MODS in 3 weeks,one died from kidney failure in 4 weeks. The other 28 patients were alive. Conclusion For these patients who suffering from extensive burn merging SIALI received therapy in early stage,we should not only schedule positive anti?shock,tracheotomy,antibiot?ics,escharectomy and nutritional support or other treatments,but also face to the challenge of lacking medical resources from too more patients. Thus,we suggest to triage the wounded to nearby hospital after anti?shock treatment,so as to improve the success rate in early stage.

Objective To investigate the correlations of plasma total homocysteine (tHcy) level and the C677T methylene tetrahydrofolate reductase (MTHFR) polymorphism with vascular dementia (VaD) and Alzheimer's disease (AD).Methods A case control study in 64 AD patients (AD group),72 VaD patients (VaD group) and 56 healthy controls (control group) was under taken.Plasma tHcy level was determined by enzymatic cycling assay.The MTHFR677C/T polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results tHcy levels were significantly higher in AD and VaD groups than in control subjects(P＜ 0.05).VaD patients also showed increased levels of tHcy as compared to AD patieuts,but no statistic significance was found(P＞0.05).Logistic regression analysis indicated that tHcy level was a risk factor for dementia (including AD and VaD together)[(OR=1.15),VaD (OR=1.23),AD (OR=1.328)].MTHFR677 TT genotype represented higher plasma tHcy concentrations than CC and CT genotypes(both P＜0.05).The distribution frequency of the T allele of MTHFRC677T was 45.3 ％ (58/128)in AD group,49.0％(51/104)in VaD group and 33.0％(37/112)in control group,and it had significant differences between the three groups (P＜0.05).There was a significant difference in distribution frequency of the T allele of MTHFRC677T between VaD and control group (P＜0.01).The distribution frequency of MTHFR677 genotype (CC/CT/TT) had a significantly difference between the VaD group and the control group(P＜0.01).Conclusions High plasma level of tHcy is closely correlated with AD and VaD.tHcy level is the risk factor for dementia (including AD and VaD together).The polymorphism of MTHFR677C/T affects the plasma tHcy level.The T allele of MTHFRC677T may correlate with VaD,while has no direct correlation with AD.

Mild stroke refers to ischemic stroke with mild neurological defects.In recent years,its incidence rate has increased gradually.However,there is no standardized definition for mild stroke,and there is a great controversy about whether to conduct thrombolytic therapy for mild stroke.The conventional wisdom holds that the patients with mild stroke should not be treated with intravenous thrombolysis;however,many studies have indicated that the short-term and medium-term outcomes are poor in patients with mild stroke without receiving thrombolytic therapy,and many patients with mild stroke may benefit from thrombolytic therapy.This article summarizes about the definition of mild stroke and the clinical studies of thrombolytic therapy in recent years,hoping to provide reference for clinicians in order to make better clinical decisions.

OBJECTIVE To investigate the effect of Protobothrops mucrosquamatus venom (PMV) and its fractions on functions of the circulatory system in vitro in order to better understand its toxicity mechanism. METHODS PMV was isolated to three fractions FⅠ, FⅡ and FⅢ with a different molecular mass range by Sephadex G-75 gel filtration chromatography. Platelet rich plasma was adjusted to 3×1011 L-1 by platelet poor plasma. Platelet suspension was incubated with PMV and its fractions 0.03 g.L-1 for 5 min, respectively, and platelet aggregation was determined on an LBY-NJ4 aggregometer. PMV and its fractions 0.05 g.L-1 were preincubated with plasminogen 0.1 U.L-1 for 10 min before chromogenic substrate cleavage activity was measured by endpoint and enzyme kinetics determination. PMV and its fractions 1.0 g.L-1 were incubated with rat plasma for 5 or 30 min, and thrombin time (TT), activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrinogen (FlB) content were assayed. The microvascular endothelial cells were exposed to PMV and its fractions 10, 50 and 250 mg.L-1 , respectively, for 24 h, while the morphological change was observed using an inverted phase contrast microscope, and the cell viability was determined by MTT method. PMV and its fractions were incubated with guinea pig red blood cell suspension in the presence or absence of lecithin for different time, and hemolysis was measured. RESULTS Compared with normal control, platelet aggregation rate was significantly increased by PMV and FⅠ (>71 ku)〔(12.4±4.1)%,(61.0±5.8)% and (56.9±5.9)%〕(P<0.01). PMV and FⅡ (18-37 ku) significantly hydrolyzed chromogenic substrate S-2251(P<0.01). PMV and FⅠ caused plasma coagulation. Compared with normal control, FⅡ and FⅢ (<10 ku) remarkably prolonged TT, APTT and PT( P<0.01). Morphological observation revealed that PMV, FⅠ and FⅡdetached the adherent cells. Compared with normal control group, PMV, F Ⅰ and F Ⅱ inhibited cell viability, and the survival rate of the cells decreased to (56.8±3.6)%,(71.6±3.8)% and(58.2±5.5)%, respectively. PMV and FⅡ slowly caused slight hemolysis in absence of lecithin. PMV and FⅡ caused significant hemolysis in the presence of lecithin, and the hemolytic rate increased to (81.0±4.0)% and (81.0±1.0)%( P <0.01) in 0.5 min, respectively, compared with (17.7±1.0)% of the control group. CONCLUSION PMV possesses different activities that affect the functions of the circulatory system in vitro, and the fractions play different roles in toxicity mechanisms.