1.Nursing of patients with pelvic fractures complicated with Morel-Lavalle′e lesions
Modern Clinical Nursing 2015;14(9):51-53
Objective To summarize the experience in nursing patients with pelvic fractures complicated with Morel-Lavalle'e lesions (MLL). Method Thirteen cases of pelvic fractures combined with MLLs were reviewed for retrospective analysis of the nursing experience. Results After subcutaneous effusion disappeared from the treatments, 13 patients underwent successfully pelvic fracture surgery. The average hospital stay was (16.0 ± 4.0) days. Conclusion The nursing measures including careful observation, tender care to patients at decubitus position, implementation of the negative pressure drainage and care at perfusion and functional exercises, can help to improve the curative effects for patients with pelvic fractures combined with MLLs.
2.The significance of electron microscopy in the etiological diagnosis of respiratory tract infection
International Journal of Pediatrics 2021;48(1):6-8
At present, electron microscopy has played a more and more important role in the field of medicine.It is often used in the ultrastructure observation of various systems, mechanism research and clinical diagnosis of diseases.The role of electron microscopy in the etiological diagnosis of respiratory tract infection cannot be ignored, but its related review is less.By using electron microscopy, the morphological characteristics of respiratory tract pathogens can be accurately observed and the new respiratory tract infection pathogens can be classified and screened.In addition, through the combination of samples with patients′ serum and observation of immuno-electron microscopy, the host response to pathogens can also be understood.However, due to the high equipment requirements, technical difficulties, complex operation and other factors, electron microscopy is rarely used in conventional etiological diagnosis, and more used in laboratory basic research.In this paper, the advantages, disadvantages and significance of electron microscopy in the etiological diagnosis of respiratory tract are reviewed.
3.Combined NFC and RFID-based innovative service in library
Juan CAO ; Yanan HAO ; Caiyu LIU
Chinese Journal of Medical Library and Information Science 2015;(7):40-43
NFC is characterized by its short transfer distance, low energy consumption, multiple operation model, high safety, portability, good interconnection, and compatibility with RFID.It can thus be considered as the updated edition of RFID.The significance to innovate the service of library using combined NFC and RFID was therefore elaborated with some suggestions put forward for constructing the smart library.
4.Effect and mechanism of Prunella vulgaris sulfated polysaccharide on angiogenesis in hepatocellular carcinoma
Yanan WANG ; Rui CAO ; Cong ZHU ; Xiongzhi WU
Chinese Journal of Clinical Oncology 2014;(12):758-761
Objective:A study was conducted to investigate the effect of Prunella vulgaris sulfated polysaccharide (PVSP) on the expression of angiogenic growth factors (bFGF, VEGF, and IL-8) and angiogenesis in hepatocellular carcinoma. Methods:ELISA as-say was used to observe the effects of PVSP and the negative control drug Lentinan (LNT, non-sulfate radical drug) on secretions of the angiogenic growth factors, namely, bFGF, VEGF, and IL-8, in HepG2 cells in vitro. In an in vivo experiment, the microvessel density in hepatocellular carcinoma tissue sections treated with PVSP and LNT was calculated, analyzed, and compared with the microvessel den-sity in the phosphate-buffered saline (PBS) control. Results:Compared with the PBS control group, PVSP at 200μg/mL inhibited bF-GF secretion (P<0.01), whereas LNT failed to affect bFGF secretion. Neither PVSP nor LNT affected the secretions of VEGF and IL-8. In vivo results showed that PVSP at 200 mg/kg reduced the microvessel density in tumor tissue sections (P=0.03), whereas LNT at 10 mg/kg failed to affect microvessel density. Conclusion:Inhibition of bFGF secretion is a probable mechanism underlying the preven-tive effect of PVSP on hepatocellular carcinoma angiogenesis.
5.Status quo of medical application software for chronic diseases management and its prospect
Zhi CAO ; Xiaomin ZHENG ; Yanan QIAO ; Xiaoli ZHA ; Wangquan XU
Chinese Journal of Medical Library and Information Science 2016;25(7):27-30,43
Objective To analyze and assess the major functions of medical APP by investigating its application in self management of chronic diseases. Methods The 3 major functions of APP were analyzed, including data record and condition assessment, medical consultation, and life therapy. Results The medical APP possesses its basic functions for chronic diseases management. However, it lacks of practicality and convenience and its homogenization is severe. Conclusion The APP +hardware +cloud medical management model should be promoted by making use of the Internet + medical technology, the supervision of mobile medical APP should be strengthened, the mobile information management mechanisms of chronic diseases should be perfected, and the self management efficiency of patients with chronic diseases should be improved.
6.Identification of Host Factors Interacting with the Movement Protein of Apple Chlorotic Leaf Spot Virus by Yeast Two-Hybrid System.
Yikun HE ; Min ZHONG ; Yu ZHANG ; Yanan WANG ; Keqiang CAO
Chinese Journal of Virology 2015;31(2):124-131
In order to identify host factors which interact with the movement protein (MP) of Apple chlorotic leaf spot virus (ACLSV), ACLSV MP was cloned into the bait vector pGBKT7 and used to screen a cDNA library of Malus sylvestris cv. R12740-7A, which had previously been constructed by yeast two-hybrid sequencing transformation. The protein functions of the identified host factors were determined according to their gene annotations in GenBank. The result showed that the bait plasmid pGBKT7-MP showed no virulence or self-activating effect on yeast strain Y2H Gold. Sixty-nine interactor proteins were identified, which were divided into the following 10 classes according to their described functions: hydrolases; pathogenesis-related proteins; DNA binding proteins; phosphatases; ligases; proteins with catalytic activity; phenylalanine ammonialyases; peroxidases; NAD binding proteins; and proteins of unknown function. Bioinformatic analysis of gene homology suggested that phosphatases, pathogenesis-related proteins and glyceraldehyde-3-phosphate dehydrogenase A may play an important role in the interaction between virus and host. This study may provide a theoretical basis for the further study of viral pathogenesis and virus-host interaction mechanisms.
Flexiviridae
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genetics
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metabolism
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Malus
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genetics
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metabolism
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virology
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Molecular Sequence Data
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Plant Diseases
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genetics
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virology
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Plant Proteins
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genetics
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metabolism
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Plant Viral Movement Proteins
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genetics
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metabolism
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Protein Binding
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Two-Hybrid System Techniques
7.Reliability and Validity of E-LINK System for Measuring Hand Strength
Yanan FENG ; Haiyan CAO ; Zhijie ZHANG ; Kuicheng LI
Chinese Journal of Rehabilitation Theory and Practice 2012;18(9):803-804
Objective To investigate the reliability and validity of the E-LINK system for measuring hand strength. Methods 25 healthy adults were recruited to measure the strength of hand using E-LINK system and Jamar respectively. They were measured again 5 d later. Results The intra- and inter-rater reliability of hand strength measurement was satisfactory (ICC>0.75), as well as validity ( Pearson r>0.75, P<0.01). Conclusion E-LINK system can measure hand strength well.
8.Expression and significance of CHOP in cognitive dysfunction in rats during intermittent hypoxia
Hongyang WANG ; Lijun DUAN ; Yaning ZHAO ; Guangchao HAN ; Min ZHANG ; Yanan WANG ; Ling WANG ; Jinli CAO
Journal of Xi'an Jiaotong University(Medical Sciences) 2015;(3):332-336
Objective To explore the expression changes of C/EBP homologous binding protein (CHOP)in intermittent hypoxic rats with cognitive function decline and clinical significance.Methods We randomly divided 75 adult male Wistar rats into normal group (NC group),5% intermittent hypoxia group (5% CIH group)and 10%intermittent hypoxia group (10% CIH group),with 25 rats in each.Then the three groups were further divided into 3 d,7 d,14 d,21 d and 28 d subgroups,and each time a subgroup had 5 rats.The control group was exposed to the air while 10% CIH group and 5% CIH group were exposed to CIH for eight hours from 8:00 to 1 6:00 each day. After exposure for 3 d,7 d,14 d,21 d and 28 d,the cognitive function of rats was assessed with Morris water maze, the expression of CHOP in the hippocampus was assayed qualitatively by immunohistochemical technique,and the apoptosis of neurons was detected by TUNEL method.Results ① With prolonged hypoxia,the time of escape latency obviously prolonged,the time spent crossing the target quadrant shortened (P < 0.05 ),and the apoptosis index of hippocampal neurons in the CIH groups was increased gradually compared with those in control group (P <0.05).The above-mentioned indexes changed more significantly in 5% CIH group than in 10% CIH group (P <0.05).②.The expression of CHOP protein at each time point was increased (P <0.05 ).In 10% CIH group it reached the peak at 28 d while in 5% CIH group it decreased after it peaked at 21 d.③ The expression of CHOP in the two CIH groups was positively correlated with apoptosis index and animal escape latency time,but negatively correlated with the target quadrant time.Conclusion Intermittent hypoxia,which is likely to induce the high expression of CHOP and activation of neural CHOP mediated apoptosis,causes cognitive impairment of various degrees.
9.Study on the phenotype and the function of CD8αα+TCRαβ+regulatory T cells
Hanxiao SUN ; Zhigang HU ; Yanan CAO ; Wenfang ZHUANG ; Binbin XUAN ; Maricic IGOR ; Huiming SHENG
Chinese Journal of Microbiology and Immunology 2014;(11):825-829
Objective To investigate the phenotype and the immunoregulatory function of CD8αα+TCRαβ+regulatory T cells in peripheral blood samples from mice.Methods The distribution profile and the phenotype of CD8αα+TCRαβ+regulatory T cells in C57BL/6 mice were detected by flow cytometry.The cytokines released by CD8αα+TCRαβ+regulatory T cells upon the stimulation with anti-CD3 antibody were analyzed by cytometric bead array.The in vitro immunosuppressive activity of CD8αα+TCRαβ+regulatory T cells on activated CD4+T cells was analyzed by using flow cytometry and carboxyfluorescein succinimidyl ester ( CFSE ) .An adoptive cell transfer assay was set up to evaluate the immunoprotective effects of CD8αα+TCRαβ+ regulatory T cells in a mouse model of experimental autoimmune encephalomyelitis ( EAE) .Results CD8αα+TCRαβ+regulatory T cells were detected in liver, spleen and peripheral blood samples collected from na?ve C57BL/6 mice.Compared with CD8αβ+TCRαβ+regulatory T cells, CD8αα+TCRαβ+regulatory T cells showed a memory-activated phenotype of CD25+CD122high CD44high CD62Llow CD69high NK1.1+DX5+.CD8αα+TCRαβ+regulatory T cells could produce IL-2 after 24 hours stimulation with anti-CD3 antibody, followed by producing IFN-γ, TNF-α, IL-4, IL-17A and traces of IL-6 and IL-10. In vitro, CD8αα+TCRαβ+regulatory T cells specifically suppressed the proliferation of activated CD4+T cells ( P<0.01 ).Moreover, they could delay the onset of EAE in mice and reduce clinical score (P<0.01).Conclusion CD8αα+TCRαβ+regulatory T cells were a unique population with immunoregula-tory function, which could be used as a potential therapeutic target in the treatment of autoimmune disease.
10.Clinical evaluation of Chen's cholangiojejunostomy for tumors around biliary-intestinal anastomosis
Guangjin TIAN ; Deyu LI ; Haibo YU ; Yadong DONG ; Yanan PENG ; Yuan CAO ; Peng LIU
Chinese Journal of Hepatobiliary Surgery 2016;22(4):265-267
Objective To explore the efficacy of Chen's cholangiojejunostojmy for tumors around biliary-intestinal anastomosis.Methods The clinical data on 11 patients operated from Jan 2012 to Dec 2014 were retrospectively analyzed.Results All these 11 patients underwent Chen's cholangiojejunostomy.The postoperative liver function significantly improved,and the symptoms of jaundice completely disappeared after operation [ALT (182.0 ±110.6) U/L vs (68.3±33.3) U/L,TBil (316.9 ±153.9) μmol/L vs (60.3 ± 25.8) μmol/L,DBil (184.1 ± 89.6) μmol/L vs (28.6 ± 12.4) μmol/L,P < 0.01;AST (195.5 ± 206.9) U/L vs (48.6 ± 21.2) U/L,GT (806.7 ± 480.0) U/L vs (204.0 ± 99.1) U/L,ALP (612.8 ±424.6) U/L vs (277.5 ± 68.7) U/L,P < 0.05].The level of CA19-9 also significantly decreased [(1 369.75 ± 1 812.18) kU/L vs (71.0 ± 46.5) kU/L,P < 0.05].There were no significant differences in the CA125,CA15-3,CEA levels [CA125 (35.3 ± 26.0) kU/L vs (29.4 ± 23.5) kU/L,CA15-3 (19.4±12.3) kU/L vs (17.9±10.7) kU/L,CEA (8.4 ±7.7) μg/L vs (7.8 ±6.6) μg/L,P > 0.05].There was no perioperative death.All the patients had a smooth perioperative recovery,except in 1 patient who developed bile leakage and another patient who had episodic attacks of cholangitis.There were no recurrent or metastatic tumors detected on follow-up.Conclusion Chen's cholangiojejunostomy was effective in the treatment of bile duct obstruction caused by tumors around biliary-intestinal anastomosis.