Objective To observe the effect of Diosmin Tablets about the prevention and treatment of edema that breaks out after a circular mixed hemorrhoids operation and the role of fibrosis on edema.Methods Having 120 cases of circular mixed hemorrhoid patients, Acco 60 cases were randomly divided into treatment group and the others were in comparison group.Both groups were outside the cut line of circular mixed hemorrhoid ligation within the injection, after these operations do conventional anti-inflammatory, hemostasia and local Ma Ying Long musk hemorrhoids ointment medication.The next day of the operation, the treatment group take a dose of Diosmin tablets 1.5 g bid 4 d, then changing the dose 1 g bid 3 d.ResultsThe incidence of edema, treatment group was 6.6% and the other group was 30%.edema in the incidence of the two groups were significantly different(P ＜ 0.01).The treatment group non-fibrosis, but the other group have 4 patients getting fibrosis.The two sets of rate about edema fibrosis were significantly different(P ＜ 0.01).Conclusion Diosmin Tablets can effectively do prevent and disperse circular mixed hemorrhoid postoperative edema.

After being introduced to Japan, the Chinese acupuncture and massage therapy has changed a lot, in which the influence aroused by the blind practitioners cannot be ignored. Through analyzing the development and the transition of the blind acupuncture and massage industry in Japan, it is found that the tube needle technique, changeable acupoints concept, technical deviation and the importance on acupoints rather than meridians are still existed commonly today, which are introduced by the blind acupuncture and massage practitioners, the special group in Japan. In the process of development, the interaction with the governmental strategy has played the essential role in the consolidation of the above features.
Acupuncture ; education ; history ; manpower ; Acupuncture Points ; Acupuncture Therapy ; history ; History, 15th Century ; History, 16th Century ; History, 17th Century ; History, 18th Century ; History, 19th Century ; History, Ancient ; History, Medieval ; Humans ; Japan ; Massage ; history ; manpower ; Visually Impaired Persons

0.05. Conclusion: The suppressive effect of MSC on patients with ITP in vitro showed no difference from that on the controls.

Objective To detect the levels of Th1 and Th2 type cytokines of peripheral blood CD+4 T lymphocytes in the patients with cervical cancer in order to find out the laboratory evidence of tumor immunotherapy. Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance expression of cytokines. Then specific fluorochrome-conjugated monoclonal antibody was added to be combined with its specific antigen. Results The level of IL-2 produced by Th1 cells were lower in patients of cervical cancer than those of normal controls, there were significant difference between them. But the level of IFN-? produced by Th1 cells had no significant change in patients of cervical cancer in comparison with normal controls. The levels of Th2 type cytokines such as interleukin-4(IL-4), interleukin-6(IL-6), interleukin-10(IL-10) were higher in patients of cervical cancer than those of normal controls, there were significant difference between them. Conclusion Th1/Th2 is imbalance in cervical cancer .Th1 type cytokines are inhibited, and Th2 type cytokines are relatively enhanced, so Th1/Th2 shifts to Th2. It may be the mechanism of tumor arising and transferring by immune escape from immunosurveillance.

High separation efficiency and fast separation speed of liquid chromatography(LC), strong sensitivity and good selectivity of mass spectrometry technology, have realized the wide use of LC and mass spectrometry technology in the field of biological macromolecules. In recent years, LC and mass spectrometry technology are gradually becoming universal in the field of vaccines, and play an increasingly important role in the quality control of vaccines. This paper summarized the application of LC and mass spectrometry technology in inactivated vaccines, vaccines of virus-like particles(VLPs), polysaccharide vaccines, and polysaccharide conjugate vaccines.

BACKGROUND:Platelet lysate has been known as a kind of lysate of autologous or alogeneic platelet-rich products. It not only removes the residual cel structure, reduces immunogenicity, but also retains many growth factors. Platelet lysate has been suggested as a substitute for fetal bovine serum to expand mesenchymal stem cels in vitro. OBJECTIVE:To observe the effects of platelet lysate on biological characteristics of human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels, and provide some experimental data for clinical cel therapy and regenerative medicine. METHODS:Platelet lysate was prepared by repeated freezing and thawing from fresh blood. Healthy adult bone marrow and adipose tissue were colected. Human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were obtained by density gradient centrifugation and type I colagenase digestion. We tested the morphology, cel phenotype, differentiation characteristics, proliferation capacity, colony forming ability and the level of cytokine secretion of bone marrow mesenchymal stem cels and adipose mesenchymal stem cels after cultured with platelet lysis. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were successfuly cultured in vitro using platelet lysate. There were no significant differences in morphology, cel phenotype, colony forming ability and the level of cytokine secretion, and chondrogenic, osteogenic and adipogenic capacities between bone marrow mesenchymal stem cels and adipose mesenchymal stem cels. Adipose mesenchymal stem cels had a high cumulative population doublings than bone marrow mesenchymal stem cels (P < 0.05). These findings suggest adipose mesenchymal stem cels had a stronger proliferative ability, and are more suitable for large-scale expansionin vitro cultivation system of platelet lysate compared with bone marrow mesenchymal stem cels.

BACKGROUND:Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells. OBJECTIVE:To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media. METHODS:A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of“mesenchymal (stromal) stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium”in English and Chinese, respectively. Final y, 41 articles were retained. RESULTS AND CONCLUSION:Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages;therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.

Objective To investigate the episode of ischemic stroke in patients with serum homocysteine (Hcy),erythropoietin and its clinical significance (EPO)levels.Methods Detected 120 cases of first ischemic stroke patients serum Hcy,EPO lev-els and test results with 120 cases over the same period outpatient healthy compare.Used Logistic regression analysis and Pearson correlation analysis to determine the relationship between serum Hcy,EPO levels and ischemic stroke,ROC curve a-nalysis of clinical characteristics of Hcy and EPO.Results Serum Hcy,EPO level of ischemic stroke were respectively 23.1 ±12.6μmol/L and 32.4±11.2 mIU/L.The control level were respectively 10.4±2.8μmol/L and 17.6±5.2 mIU/L.Is-chemic stroke group were significantly higher.The results had statistical significance (P<0.05 or<0.01).Pearson correla-tion analysis showed that ischemic stroke serum Hcy levels and EPO levels were positively correlated (r=0.369,P<0.05). Logistic regression analysis showed that Ischemic stroke was positively related to serum Hcy level (P<0.05),and were not related to EPO (P>0.05).Conclusion The serum Hcy,elevated EPO levels associated with ischemic stroke.Serum Hcy is an independent risk factor of ischemic stroke.

Low-density lipoprotein receptor-related protein-1 (LRP-1) is a transmembrane receptor protein locatedon the plasma membrane of the cells and involved in receptor-mediated endocytosis.LRP-1 binds to distinct ligandsthat are structurally and functionally unrelated, which makes it not only mediates endocytosis, but also regulates cell surfaceproteolytic activity and specific intracellular signaling pathways related to multiple links in the process of developmentof atherosclerosis.Moreover, LRP-1 plays an important role in maintaining vascular stability.This review focuses on theprogress in LRP-1-regulated vascular integrity, and provides new insights to the target of the blood vessel diseases.

Objective To study the effects of glucocorticoid receptor (GR) signal pathway and downstream cytokineson androgen-independent growth of prostate cancer (PC) cells.Methods The human androgen-dependent PC (ADPC) cell line LNCaP and androgen-independent PC (AIPC) cell line DU145 were cultured in vitro.Immunocytochemistry was used to examine the expressions of the androgen receptor (AR),GR,HSPg0 and interleukin-6 (IL-6).The GR antagonist RU486 was used to treat cultured cells,and the effects of RU486 on the proliferation of both cell lines were analyzed by MTT assay.Expressions of HSP90 and IL-6 mRNA and protein were assessed by RT-PCR and Western blotting respectively.Results LNCaP cells were AR-positive and GR-negative,whereas DU145 cells were GR-positive and AR-negative.The expressions of HSP90 and IL-6 were significantly stronger in DU145 cells than in LNCaP cells (P＜0.01).RU486 had no obviously effects on the growth of LNCaP cells,but exerted a significant time-and dose-dependent growth inhibition on DU145 cells.RU486 treatment in DU145 cells also resulted in a dose-dependent decrease in the expressions of HSP90 and IL-6 mRNA and protein.Conclusions GR signal pathway may be the main survival pathway for DU145 cells.Abnormal hyperactivation of GR signal pathway and its promoting the expressions of HSP90 and IL-6 may contribute to the progression of ADPC to AIPC after androgen ablation.