Objective To discuss the advantages of combined use of intravenous indwelling needle and infusion connector joint.Methods 120 cases of children in our hospital receiving chemotherapy through intravenous infusion were randomized into the control group and the experimental group with 60 cases in each group.The control group used heparin cap,after each infusion was completed,with 12.SU/ml 3～5ml of heparin saline pulse-type sealing.The experimental group adopted infusion connector joint,after the infusion completed,3～5 ml normal saline was injected into the joint to flush the blessing sealed tube.The complications rate was calculated in two groups.Results The experimental group and the control group showed differences in the incidence of complications and the patients' approval to nurses.Conclusions Combination of intravenous indwelling needle and infusion connector joint can reduce the complications significantly,and improve patients' approval to nurses.

AIM:To observe the effects of advanced glycation end-products (AGEs) on thioltransferase (TTase) expression and activity in human lens epithelial cells.
METHODS: Human lens epithelial cells B3 ( HLE B3 ) were treated with 1. 5mg/mL AGEs - BSA as the experimental groups cultured by fetal bovine serum of volume fraction 10% dulbecco modified eagle medium ( DMEM) and bovine serum albumin ( BSA) was added at the same concentrations as the negative control. The level of reactive oxygen species ( ROS ) was evaluated. Cells were collected at 0, 1, 2, 3, 4d and total RNA or protein was extracted. TTase mRNA levels were detected by qRT-RCR. TTase expression was detected by Western blot and its activity was measured.
RESULTS: Compared with the control group, AGEs-BSA up-regulated the expression of ROS (P<0. 01), ROS content increased in a time-dependent manner. BSA had no effects on ROS expression. The expression of TTase increased after treatment with AGEs-BSA for 1d, peaked at 2d (nearly 5. 06-fold increase, P<0. 01), then decreased gradually. No change was observed between BSA and control group (P>0. 05). Similarly, TTase activity peaked at 3d (nearly 2. 01-fold increase, P<0. 01). Western blot test found that TTase protein expression was increased
gradually, starting from the 3d TTase expression was reflected that there was statistically significant difference compared with control group (P<0. 05).
CONCLUSION:AGEs-BSA significantly increases the production of ROS in human lens epithelial cells, and it then induces the oxidative stress which may promote the expression of TTase and enhances the activity of TTase.

Objective To explore ladder and compensation system of nurse-staffing which is suitable for the situation of our country.Methods The ladder management and compensation system to nursestaffing of National Health Service (NHS) in UK were given detailed introduction and analysis through the official documents,references and personal study experiences,and summarize its scientific and referenced aspects.Results NHS has formed a set of mature competency-based ladder system,and linked the ladder system to compensation system,made a relative scientific performance appraisal system,realized the scientific competency matches the level,salary changes along with the level model.Conclusions It provides references and thinking to the in-depth reform of public hospitals in our country,and the development of high quality nursing service,as well as the implementation of nursing ladder management and compensation reform.

Obfectives:The purpose of the present study was to compare the effects of different early enteral nutrition formula (containing either MCT/LCT or LCT only) on improving nutritional status of burned patients. Methods:This was a prospective,randomized,single blind and controlled study.Thirty cases of burned patients injured by varous causes with total burn surface area (TBSA) more than 30% were collected and randomized into two groups:①Group F:enteral feeding with Fresubin 750 MCT containing MCT/LCT as fat source,②Group N:enteral feeding with Nutrison,containing only LCT as fat source.All of the thirty patients received isocaloric,isonitrogenous,and isolipidic enteral feeding,with intragastric tube and enteral feeding pump,started within 24 h postburn.The several parameters were observed on postburn day(PBD) 1,4,7 and 10,such as serum concentrations of transferrin and prealbumin and plasma concentrations of prostaglandin E 2(PGE 2) and insulin.Nitrogen in urine per day and excretion of 3 methylhistidine in urine on day 1,7 and 10 postburn were determined. Results:The results showed that the patients of F group had an increased blood concentration of transferrin,prealbumin and insulin,and a decreased plasma concentration of PGE 2.Excretion of nitrogen and 3 methylhistidine in urine was also markedly reduced in patients fed with nutrients containing MCT/LCT compared with LCT. Conclusions :We have shown that an enteral diet containing MCT/LCT can improve the nutrition status of burned patients more effectively.

Objective: To study the correlation of magnetic resonance imaging (MRI) features with the tumor nuclear DNA contents, and evaluate the biological behavior and proliferative potential of meningiomas using MRI. Methods: The DNA contents and the incidence of aneuploid of 45 patients with meningiomas were measured by means of photodensity method with image cytometry. The data were compared with MR findings for the statistical correlation analysis. Results: Tumors with the presence of peritumoral edema, irregular tumor shape, and ambiguous brain-tumor border had remarkable difference in DNA contents compared with those with negative peritumoral edema, smooth tumor shape, and clear brain-tumor border (P < 0.01). Using Spearman' s correlation coefficient analysis, we found a significant correlation (r = 0.696, P = 0.000) between three of MRI scores calculated for each patient and DNA contents. Conclusion: DNA contents and the incidence of aneuploid are higher in the tumors with the presence of peritumoral edema, irregular tumor shape, and ambiguous brain-tumor border. All of the imaging appearances can be used to estimate the proliferation potency and malignant biological behavior of the meningiomas.

Objective:To study the relationship between pre-pregnancy body mass index (BMI)and preterm birth.Methods:A case-control study was conducted in Haidian Maternal and Child Health Hos-pital in Beijing from January to April in 201 3.This study contained 1 74 preterm births in the case group and 382 term deliveries in the control group.The height,pre-pregnancy body weight,body weight before delivery,gestational weeks,history of diseases,family history of diseases,and complications during pregnancy of the subjects were collected.Multivariate Logistic regression was used to estimate the odds ratio and 95% confidence intervals (CI)after adjustment by maternal age,education,smoke during pregnancy,primiparous,mean income,and mean family living space.Results:After analyzing the rele-vant risk factors of preterm birth,the multivariate Logistic regression showed that pre-pregnancy obesity was a risk factor for preterm birth,the adjusted odds ratio was 2.461 (95% CI:1 .1 74 -5.1 59,P =0.01 7).The associations between pre-pregnancy overweight and preterm birth or pre-pregnancy under-weight and preterm birth were not found.The gestational diabetes mellitus,pregnancy-induced hyperten-sion,and family history of preterm birth were risk factors for preterm birth,the adjusted odds ratios were 1 .781 (95% CI:1 .025 -3.095,P =0.040),3.831 (95% CI:2.044 -7.1 80,P <0.001 ),and 3.675 (95% CI:1 .358 -9.942,P =0.01 0),respectively.Conclusion:Pre-pregnancy obesity ap-peared to be a risk factor for preterm birth.To decrease the incidence of preterm birth,women should improve preconception care and keep their BMI in a normal range before pregnancy.

Dental Porcelain ; classification ; Humans

Autoantibodies ; blood ; immunology ; Child ; Diagnosis, Differential ; Humans ; Radiography ; Radionuclide Imaging ; Thyroid Diseases ; blood ; diagnosis ; diagnostic imaging ; Thyroid Function Tests ; methods ; Thyroid Gland ; diagnostic imaging ; immunology ; Thyroid Hormones ; blood

Objective To analyse the cause of misdiagnosis of tuberculosis peritonitis and discuss feasibility for diagnosis of tuberculosis peritonitis by laparoscopy. Methods 12 patients misdiagnosed as tuberculosis peritonitis were retrospectively analysed. Results Tuberculosis peritonitis wrongly diagnosed because of atypical clinical behaviors in spite of specific laboratory examination. However, laparoscopy could diagnose tuberculosis peritonitis exactly and quickly. Conclusion Laparoscopy is an effective method of diagnosis for tuberculosis peritonitis.

Background Patients' experience during phacoemulsification cataract surgery is an important determinant of patient's satisfaction with surgery effectiveness.Local anaesthesia could be associated with different intraoperative visual awareness because of retention of optic nerve function.Relieving the anxiety of patients arose from intraoperative visual awareness is of important clinical significance.Objective This study was to discuss the necessity of preoperative conversation and psychological counseling and to improve the quality of surgery and the satisfaction of patients.Methods One hundred and fifty-three patients who had undergone sequential phacoemulsification and intraocular lens (IOL) implantation in the same operation under local anaesthesia from October,2010 to December,2010 in Tangdu Hospital and Xi'an First People's Hospital,received a standardized questionnaire designed based on similar questionnaires from abroad and surveyed regarding their intraoperative visual experience,including light perception,their detection of color,photopsia,and their detection of the movement of the surgeon,instrument,and changes in light intensity during cataract surgery.In another survey,seventy ophthalmologists in the Xi'an area with ≥ 10 years (33) and ＜ 10 years of experience (37) participated in a questionnaire about their recognition and response to these visual experiences,designed based on the 2010 Chinese Doctors General Questionnaire.Results During the phacoemulsification and IOL implantation,27 patients (17.6％) lost light perception,98 patients (64.1％) had light perception,28 patients (18.3％) detected more than one color,25 patients (16.3％) had photopsia,49 patients (45.1％) sensed the movement of the surgeon,26 patients (17.0％) was able to see surgical instruments,11 patients (7.2％) saw the surgeon's fingers,4 patients (2.6％) saw the surgeons,65 patients (42.5％) detected changes in light intensity and 12 patients (7.8％) saw the IOL loop and others.21.4％ of the 70ophthalmologists believed these patients might have lost light perception;while 78.3％ thought these patients retained light perception.Under local anaesthesia,93.9％ of the more experienced surgeons and 75.6％ of the junior surgeons deemed that good preoperative counseling was helpful for the patients,but only 45.5％ of the more experienced surgeons and 24.3％ of the junior surgeons counseled patients regarding intraoperative visual experience.Conclusions Most patients will experience a variety of visual sensations during phacoemulsification and IOL implantation under local anaesthesia.Many ophthalmologists are aware of these conditions.Most of the ophthalmologists believe that patients suffer from fear from these intraoperative visual experiences and claim that preoperative counseling to patients can relieve that fear,but less of them communicate these possible experiences to patients.This study offers some discussions about preoperative counseling.