Objective To investigate unpaid donors of zaozhuang city on AIDS knowledge ensure the safety of blood.Methods 3 842 unpaid donors donating blood to blood-bank were randomized to the anonymous question-naire survey of AIDS basic knowledge ,practical recycling questionnaire on the spot 3 576(93.08%).Questionnaire on AIDS knowledge,attitude and effective was 3 358(87.40%);The sexual behavior effective questionnaire was 1 201(31.26%).The original data was put into Excellto statistical analysis and processing .Results The situation of understanding the knowledge of HIV/AIDS,understanding two ways of blood and sexually transmitted were 86.52%and 80.23% respectively;Understanding of mother-to-child vertical transmission accuracy was only 50.67%;The right to use condoms to prevent the spread of HIV was 74.71%;Accuracy up to 74.75%of prostitution susceptible to HIV/AIDS;Patients who were only prevented but couldn′t be cured of HIV was 93.26%.Blood donors before dona-ting blood,donate blood once and donate twice correct awareness were significantly improved .About the HIV/AIDS can infectious,blood transfusion could pass and be transmitted through sexual transmission ,mother-to-child transmis-sion,embrace or kissing transmission ,intravenous drug use ,condom could prevent ,could be cure the recognition accu-racy rate was(26.57%,64.51%,78.83%),(51.54%,80.12%,89.61%),(61.41%,73.57%,86.82%), (59.56%,77.63%,88.79%),(30.54%,68.48%,72.40%),(59.83%,70.21%,85.62%),(64.58%, 81.27%,93.56%),(68.59%,84.63%,85.28%),Before the blood supply and after donate blood twice the aware-ness for HIV/AIDS were significantly increased,there was a positive correlation between(all P<0.05).Unpaid blood donation AIDS knowledge source of propaganda materials and donating blood (43.67%),newspaper and magazine (28.12%),radio and television(2.71%),5.60% family friends and colleagues.Conclusion Unpaid donors to master a certain knowledge of HIV/AIDS;Carry out AIDS health education and intervention of epidemiology ,is to en-sure that the citizens to donate blood safety preconditions .

Objective To discuss the nursing measures of patients with laparoscopic radical hysterectomy and pelvic lymph node dissection. Methods 24 patients with laparoscopic radical hysterecto my and pelvic lymph node dissection were selected and their perioperative nursing measures were summa rized. Results 24 patients were all cured and discharged, 6 patients accepted chemotherapy and 3 patients accepted radiotherapy. All patients were followed up, and they recovered well. Conclusions Perfect perioperative can guarantee early recovery of patients.

Using therapeutic letters in psychotherapy is a common trend among different psychotherapy schools.Narrative therapy applies therapeutic letters most frequently among these schools.The article introduces the purposes,styles,key points of writing,the differences with case record,the progress of application and research and the localization of narrative therapeutic letters in order to develop the application and localization of therapeutic let-ters in China.

The interlink,consistency and unity of all things is an important thought in traditional Chinese philosophy.Therefore,holism of human being and universe is the long-dominant thought in China.Traditional Chinese medicine(TCM)forms its theory based on this holism concept.From the perspect of history and philosophy,the difference between TCM and western medicine was discussed after retracting the influence of the holism of human being and universe on TCM.Holism of human being and universe has a profound impact on TCM,which also contributes to differences between TCM and western medicine.

More and more research proved that CD4+CD25+ regulatory T cells (Treg cells) play an important role in immune tolerance in these years. Known as a T cell growth factor, Interleukin2 (IL-2) regulated the immune tolerance induced by Treg cells. IL-2 sustained the activity of Treg cells both in thymus and periphery while it was dispensable for the development of Treg cells in thymus. In addition, IL-2 signal affected the function of Treg cells and maintained their competitive fitness. Therefore, CD4+CD25+ Treg cells and IL-2 formed an immune network to regulate the processes of immune response, immune tolerance and autoimmune homeostasis.

[Summary] Stress response is an organism ’ s mechanism of defense against various stimulations , involving nervous , endocrine , cardiovascular and immune systems .This review summarized the clinical research progress on effects of different anesthesia management on stress response and future trends of investigation .

OBJECTIVE:To understand the status of the use of national essential medicines,and provides reference for further implementing the national essential medicine system and promoting the priority use of national essential medicines. METHODS:Ac-cording to the statistics analysis of the use of national essential medicines(for example the antihypertensives and oral hypoglycemic agents)in Beijing,Shanghai and Guangzhou areas,the related influential factors about priority use of national essential medicines were explored. RESULTS:The proportions of use amount of national essential medicines in our hospital in 2012-2013 to the total amount of medicines were 21.20% and 18.75%,respectively,with a downtrend;the proportion of antihypertension drugs to total amount was only 14.05%and 13.70%,respectively;compared with the same generic drugs with individual pricing,the GMP prod-ucts of Valsartan capsule,Bisoprilol tablet and Amlodipine tablet had no advantages in DDDs,or even lower. DDDs of individual pricing drugs of Acarbose tablet in medical institutions in Beijing area and tertiary hospitals in Shanghai and Guangzhou area in 2011-2012 were much higher than the generic GMP products with the same generic drugs. CONCLUSIONS:The clinical use of na-tional essential medicines in our hospital and parts of the country still remains to be further improved,the influential factors includ-ed reimbursement mechanism,awareness rate of related knowledge,physicians’habit to drug use and pursuing economic benefits. The propaganda and training should be strengthened,awareness rate of medical staff and publics to national essential medicines, the availability of national essential medicines and national essential medicine system and medicare reimbursement payment policy should be improved,and the priority use of national essential medicines should be promoted by more measures combination use.

Iodine is the essential element of the human body. Both of iodine deficiency and iodine excess can produce different biological effects on organisms in various degree . It not only can damage the thyroid gland but also influence the nervous system and oxidize system, even damage hereditary material and immune system. The studies of the different biological effects of iodine deficiency and iodine excess on the cells and organisms can play an important role in unveiling the mechanisms of iodine deficiency disease and iodine excess disease. It also can offer the solution for preventing and curing the relevant disease of iodine.

Objective To observe the clinical efficacy of acupuncture plus Chinese herbs in treating sensorineural hearing loss.Method Eighty patients with sensorineural hearing loss were randomized into a treatment group and a control group, 40 cases in each group. The control group was intervened by conventional medication, while the treatment group by acupuncture in addition to the intervention given to the control group. The two groups were intervened once a day, 30 days as a treatment course. The serum interleukin (IL)-2, IL-6, soluble interleukin-2 receptor (sIL-2R) levels and pure tone hearing threshold value were observed before and after consecutive 2 treatment courses, and the clinical efficacies in the two groups were compared.Result The serum levels of IL-2, IL-6 and sIL-2R and pure tone hearing threshold value were significantly changed after the treatment in the two groups (P<0.05). There were significant differences in the serum IL-2, IL-6, sIL-2R levels and pure tone hearing threshold value between the two groups after the treatment (P<0.05). The total effective rate was 94.6% in the treatment group versus 84.2% in the control group, and the difference was statistically significant (P<0.05).Conclusion Acupuncture plus Chinese herbs is an effective approach in treating sensorineural hearing loss.

OBJECTIVE:To establish a RP-HPLC method for determination of the content of hesperidin in weisu gran?ules.METHODS:Symmetry C 18 (150mm?3.9mm,5?m)column;acetonitrile-water(19.5∶80.5),water containing phosphoric acid(800∶0.1)as mobile phase and detection wavelength was284nm.RESULTS:The calibration curve was linear in sample size range of hesperidin of0.0228?g～0.1596?g(r=0.9996).The average recovery was97.2%(RSD=1.1%,n=6).CONCLU_ SION:The method was simple and accurate,and can be used for quality control of weisu granules.