OBJECTIVE:To prepare rapid-disintegrating oral tablets of indometacin and establish a method for its quality control.METHODS:The formula of the oral tablets was optimized by orthogonal test with the formula quality of CMS-Na(A),L-HPC(B),MCC(C),manniton(D) as factors,and the disintegrating time as index.The content of indometacin was determined by ultraviolet spectrophotometry.RESULTS:The optimal formula of the tablets was the following,A∶B∶C∶D=5∶5∶50∶5.The preparation was white in color and its quality was up to the related standard specified in Chinese Pharmacopeia (2005 edition) in identification.The linear range of indometacin was 2.08～41.6 ?g?mL-1 (r=0.999 9).The average recovery was 99.3%.The intra-day RSD=0.53% and inter-day RSD=0.74%.CONCLUSION:The preparation is simple and feasible in preparation process,and controllable in quality.

OBJECTIVE:To investigate the inhibitory effect of ligustrazine on the formation of macrophage-derived foam cells and its possible mechanism. METHODS:Human acute mononuclear cells (THP-1) were incubated with 160 nmol/L phorbol ester (PMA) for 24 h to differentiate into macrophages;and the macrophages were incubated with oxidized low-density lipoprotein (ox-LDL)culture solution containing 80 mg/L for 24 h to differentiate into macrophage-derived foam cells. And then the cells were randomly divided into blank control group(ox-LDL),model(AngⅡ)group,positive control(valsartan)group,and ligustrazine low,medium and high concentration groups(the mass concentration were 0.025,0.05 and 0.1 g/L). After all cells were respective-ly incubated with 80 mg/L ox-LDL culture solution for 48 h,oil red O staining was adopted to observe the transformation rate of foam cells,enzyme chemical method was used to determine the content of cholesterol,and real-time quantitative polymerase chain (RT-PCR)and Western blot were conducted to detect expression levels of Acyl-coenzyme A cholesterol acyltransferase-1(ACAT-1) mRNA and its protein. RESULTS:Compared with blank control group,the transformation rate of foam cells and content of choles-terol in model group were increased,and the expression levels of ACAT-1 mRNA and its protein were obviously strengthened,with significant differences(P<0.01 or P<0.05). Compared with model group,the transformation rate of foam cells and content of cho-lesterol in positive control group(valsartan)and ligustrazine low,medium and high concentration groups were decreased,and the expression levels of ACAT-1 mRNA and its protein were obviously weakened,with significant differences (P<0.01 or P<0.05). CONCLUSIONS:Ligustrazine can inhibit the macrophages differentiating into foam cells,by a mechanism that may be related to inhibiting expression of ACAT-1,and reducing content of cholesterol to reduce formation of foam cells.

OBJECTIVE To discuss the hospital infection management in infectious disease ward,to improve the ward quality of hospital infection,and to prevent and control infection inside hospital.METHODS By the plan,do,check and action(PDCA) circle method to collect information,analyze cause,establish plans,and organize to put into practice and check management.RESULTS Making the control quality of hospital infection improved obviously,various index signs attain the Hospital infection management norm and The disinfection technique norm issued by Ministry of Health and infection managment standard requirement for third class grade A hospitals.CONCLUSIONS Inducting PDCA circle method and step by step to insist on it is a good method to improve the control quality of hospital infection.

Objective To evaluate the effect of nasal continuous positive airway pressure (nCPAP) in pediatric patients with respiratory failure after liver transplantation.Method A prospective? randomized controlled clinical trial was conducted during June 1st 2013 to June lst2015 in 71 pediatric patients with respiratory failure after liver transplantation.A total of 66 patients completed the trial and 5 patients quitted.Conventional oxygen therapy group included 35 cases and nCPAP group included 31 cases.The vital sign,blood-gas analysis,intra-abdominal pressure and prognosis were compared between the two groups.Result After nCPAP treatment,the heart rate,respiratory rate,and PCO2 decreased at 4 and 24 h (P＜0.05),oxygenation index was improved at 24 and 48 h (P＜0.05),and intra-abdominal pressure decreased at 48 h (P＜0.05).nCPAP showed better outcome than conventional oxygen therapy (P＜0.05).The incidence of refractory atelectasis,intra-abdominal hypertension,and epilepsy was higher in treatment failure population.Conclusion nCPAP is more suitable for pediatric patients with respiratory failure after liver transplantation,especially for those accompanied with intra-abdominal hypertension.

Community-acquired methicillin-resistant Staphylococcus aureus ( CA-MRSA ) has been increasingly frequently isolated from patients worldwide, and is an emerging threat to public health.CA-MRSA strains differ from hospital-acquired MRSA strains in their epidemiologies, clinical features and genetic backgrounds, and the predominant CA-MRSA strains vary between geographic settings.This paper reviews literatures on the definition, epidemiology, clinical manifestations, treatment and virulent factors of CA-MRSA or CA-MRSA infection, and points out that we should further promote studies on epidemiology, molecular genetic properties of CA-MRSA and clinical management of CA-MRSA infection.

Objective To explore the application effect and safety of ultrasonic location in interscalene bra-chial plexus block anesthesia.Methods 100 cases with upper extremity orthopedic surgery patients were selected, according to the brachial plexus between the different positioning methods,they were divided into control group and observation group,50 cases in each group,the control group chose the traditional anatomical localization techniques, observation group interscalene under ultrasound guidance brachial plexus block,two groups of anesthesia were com-pared,block success rates and complications were observed.Results The block operation time,anesthesia onset time,duration of analgesia and anesthesia drug dosage of the observation group were (185.5 ±24.86)s,(11.55 ± 2.89)min,(11.42 ±2.39)min,(17.25 ±2.54)mL while the control group were (228.75 ±26.20)s,(16.05 ±4.66)min,(10.95 ±2.83)min,(19.50 ±2.79)mL,there was significant difference between two groups(t =18.34, 10.28,9.72,10.68,all P <0.05).the anesthesia success rate of the observation group was 98%,significantly higher than 88% of the control group,the difference between the two groups was statistically significant(χ2 =9.12,P <0.05).The incidence rate of complications in the observation group was 0,while the control group was 8%,two groups had significant difference in complication rate(χ2 =8.34,P <0.05).Conclusion Ultrasonic location in in-terscalene brachial plexus block anesthesia has a good clinical effect,with high safety,which is worthy of populariza-tion and application.

Objective To investigate elder′s subjective well‐being(SWB) before they move into nursing home ,so as to pro‐vide reference for personalized care plan .Methods Basic information scale ,Memorial University of Newfound land Scale of Happi‐ness(MUNSH) ,Activities of Daily Living (ADL) ,UCLA Loneliness Scale (UCLA) ,and Center for Epidemiologic Studies Depres‐sion Scale(CES‐D) were used to investigate 162 aged people .Results There was no significant difference in gender ,career ,educa‐tion background and pension(P> 0 .05) .In 71 and above age group ,the SWB was higher than in the 60 - 70 age group(P< 0 .05) and in marriage group ,it was higher than the divorced group and widowed group (P< 0 .05) .SWB was positively related to self‐care ability ,in completely normal group and decreased function group were also statistically higher than what in obvious function obsta ‐cle group(P< 0 .05) .The score of SWB was negatively correlated with loneliness and depression scores .In normal group ,it was higher than loneliness group(P< 0 .05) .As the depression score increased ,the score of SWB gradually decreased .Compared with the normal group ,SWB in depression group ,maybe depression group and critical group decreased markedly (P< 0 .05) .Conclusion Elders′ SWB is influenced by many factors .Besides providing life care ,more attention should pay to the mental health of old people , giving them personalized care .

Vulnerable plaque (VP) has been widely considered as the main cause of acute coronary syndrome (ACS) and cardiac death .In recent years ,invasive or non-invasive intravascular ultrasound imaging has become one of main measures for detecting VP .The present article made following overview on advantages and disadvantages of intravascular ultrasound imaging assessing VP .

ABSTRACT:Objective To investigate the effects of poly ADP-ribose polymerase (PARP ) inhibitor 3-aminobenzene (3-AB)on the delayed development cerebral vasospasm (DCVS)after subarachnoid hemorrhage (SAH)and on the inflammatory factors,namely monocyte chemotactic protein 1 (MCP-1 )and hypersensitive c-reactive protein (hsCRP),and to explore the relationship between these and the signaling pathway of NF-kappa B (NF-κB).Methods Eighty male Sprague-Dawley rats were randomly divided into four groups:normal group (n =8),sham-operation group (n =8),SAH model group (n =32)and 3-AB group (n =32).We established 64 SAH model animals by double injection of blood into the cisterna magna.Half of the SAH model animals were treated with 3-AB by intraperitoneal injection (30 mg/kg).These rats were killed to obtain specimens respectively at days 3, 5,7 and 14 after the second blood injection.The morphological changes of basilar arteries were observed under the light microscope.The contents of PARP,MCP-1 and hsCRP in brain tissues were detected with enzyme-linked immunosorbent assay (ELISA).The expression of NF-κB in basilar arteries was determined by immunohistochemistry.
Results Compared with those in the sham-operation group,the degree of basilar artery spasm reached the peak [(30.47±3.89)%]at day 5 after established SAH model;the thickness and diameter of basilar artery were (1 6.44 ±1.32)μm and (1 78.21 ± 1 1.13)μm,respectively.Cerebral blood flow was reduced by nearly 60% (P <0.01 ). The expression of NF-κB in the cytoplasm and nucleus and PARP content in brain tissue were both increased significantly (P < 0.01 ).MCP-1 [(365.29 ± 28.08 )pg/mL ] and hsCRP [(402.1 6 ± 48.99 )ng/mL ] were significantly enhanced (P <0.01).Compared with the SAH group,after 5 days’intervention with 3-AB,there was obvious alleviation in the spasm degree of basilar artery [(22.65±3.21)%],the thickness [(14.89±1.27)μm]and diameter [(1 98.56±10.91)μm],respectively (P <0.01).Cerebral blood flow was significantly enhanced,but the expression of NF-κB in the cytoplasm and nucleus was decreased and PARP in brain tissue was significantly decreased (P < 0.01 ).MCP-1 [(126.5 1 ± 18.67 )pg/mL]and hsCRP [(285.39 ± 39.07 )ng/mL]in brain tissue were significantly declined,respectively (P <0.01).Conclusion PARP inhibitor 3-AB can alleviate DCVS and inhibit the inflammatory response in brain tissue after SAH.The mechanism may be related to NF-κB signaling pathway.

To a certain extent, itemized government subsidies to public hospitals, primarily used for hospital infra-structure and large equipment investments, can alleviate the financial pressure of development expenditures and thus re-duce the medical expenditure burden on patients. To test the hypothesis, the panel data method has been adopted based on the data of 23 tertiary hospitals. Empirical results find that:(1) increases in itemized subsidies will reduce the burden of medical expenses on patients;(2) conversely, the impact of itemized subsidies on outpatient medical expenses is more sig-nificant. By sharing a portion of public hospital expenses and impacting hospital service providing behavior, itemized sub-sidies help to reduce the burden of medical expenses on patients.