The initiation and development of diabetic chronic complications is associated not only with the level of blood glucose,but also with the glucose fluctuation.The damage in large artery and microvessel may be caused by glucose fluctuation.There are two causes which induce glucose fluctuation in clinical:one is postprandial hyperglyceamia with not well controlled,the other is hypoglycemia causes by unsuitable therapy.A lot of effective new types of substances were applicated in clinical,such as ?-glucosidase inhibitor,glinide-insulin secretagogus,fast-acting and long-acting insulin analogues.With these new powerful tools,there is possibility to treat diabetes more correctly and safely,and control the blood glucose in a stable and good level.

Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

The incidence of malnutrition among patients with chronic kidney disease (CKD) constantly increases despite the development of health care and dialysis techniques.New nutritional targets for CKD patients upon maintenance treatment have been developed.Before dialysis,evidences have shown that a long-term nutritional care plan,with a control of protein intake,can effectively correct metabolic disorders including proteinuria and acidosis.During the maintenance dialysis,the optimal protein and energy intakes have been recently challenged.The new classification of nutritional disorders in CKD patients may help the easily identification of protein-energy wasting.

Humans ; Intraoperative Complications ; Osteotomy ; adverse effects ; methods ; Postoperative Complications ; Spine ; surgery

Humans ; Minimally Invasive Surgical Procedures ; Spine ; surgery

Humans ; Osteotomy ; adverse effects ; methods ; Scoliosis ; surgery

Arthroplasty, Replacement, Hip ; trends ; China ; Hip Prosthesis ; Humans ; Prosthesis Failure

Community Language Learning was employed to strengthen English class cohesion and improve students performance, and positive results have been achieved with regard to classroom atmosphere, students involvement and CET-4 scores. Initial studies and experiments show that application of foreign linguistic teaching theories in the Chinese setting is feasible and rewarding.

Objective:To construct a eukaryotic vector containing human TACI-linker-BR3-IgGFc double receptor fusion gene and to investigate its expression in COS-7 cells.Methods: The standard cloning technology was employed to construct the(title) vector,which was then used to transfect COS-7 cells by lipofectamine 2000.The expression of target protein and their interaction with BAFF and APRIL protein were examined by Western blot,ELISA,and immunohistochemistry methods.Results: The expression of the fusion protein was observed in transiently transfected COS-7 cells, with the transfection efficiency being 48%.The fusion protein was detected in the supernatant.It suggested that the fusion protein interacted well with BAFF and(APRIL) proteins;the IgGFc tag did not influence the natural assembly and the biological activity of fusion proteins.Conclusion: A vector containing TACI-linker-BR3 double receptor and IgGFc fusion gene has been successfully constructed and the(subsequent) fusion protein is bioactive,which paves a way for further research on the pathogenesis of autoimmune diseases and biotherapy strategy.

Objective To clone mouse FasL(mFasL) full length cDNA and construct eukaryotic expression vector,and to detect its expression in dendritic cells.Methods RT-PCR and TA cloning technique were used to clone the mFasL full length cDNA from mouse activated T lymphocytes,and then eukaryotic expression vector pIRES2EGFP containing mFasL cDNA was constructed.The positive recombinants pIRES2EGFP-mFasL were transfected into dendritic cells(DC),the expression of mFasL mRNA was detected by RT-PCR and the expression of mFasL protein was assayed by immunofluorescence and Western blotting.Results The cloned full reading frame of mFasL cDNA was in coincidence with the sequence registered in GenBank.In the DC after transfection of pIRES2EGFP-mFasL,the expressions of mFasL mRNA and mFasL protein were detected.Conclusion The mFasL cDNA was cloned and constructed into the eukaryotic expression vector successfully,and mFasL gene could be expressed efficiently in DC transfected by pIRES2EGFP-mFasL.