Reference service is one of the key tasks in library.The current situation of reference service in library and its problems were analyzed from the need of readers, and the problems to which attention should be paid were pointed out from its convenience and time-effectiveness .

Viral myocarditis can cause severe myocardial injury and even acute heart failure, then evolve into dilated cardiomyopathy, known as inflammatory cardiomyopathy. Immune reaction plays a key role in the transformation from viral myocarditis into dilated cardiomyopathy, including virus RNA continuous replication,immune-mediated injury, cellular apoptosis, other cellular immune response , myocardial antibodies and other humoral immune response. In the clinical treatment, besides anti-heart failure and cardiac nutrition, the use of ribavirin, α1-receptor blockers, β-receptor blockers, calcium channel blockers, angiotensin converting enzyme inhibitors and spironolactone should be emphasized.

Objective To recommend the appropriate retrieval tools for scientific researchers and improve their re-trieval efficiency by comparatively analyzing the retrieval results from PubMed and Embase .com.Methods Eight retrieval terms from different subjects were used to retrieve PubMed and Embase .com by subject headings and basic retrieval methods than can automatically transfer their function .Results Six different results were retrieved from PubMed and Embase.com with 8 retrieval terms.Conclusion The recall ratio is higher in Embase.com than in PubMed when they are retrieved by subject headings while the accuracy ratio is higher in PubMed than in Embase . com when they are retrieved by basic retrieval methods than can automatically transfer their function although error transformation occurs in both PubMed and Embase.com.

Objective To understand the learning needs of nursing students for nursing English in senior high vocational school.Methods A survey was carried out among 982 three-year nursing students in grade 2007-2009 in Cangzhou medical college by questionnaire.The data collected were analyzed using descriptive statistics with SPSS 17.0.Results Most students (93.0％) believed that nursing English was important and had a strong desire for most English skills,especially speaking and listening ability.They (90.7％) expected nursing teachers to take charge of or participate in teaching work and some students (30.6％) were dissatisfied with teaching material.They depended on teachers' instruction and preferred learning in real situations.Conclusions Nursing students in senior high vocational school have strong needs for nursing English,but there is a gap between current teaching situation and learning needs,which the course designers and teachers should take into full consideration in order to make the teaching more effective.

With the development of clinical pharmacy in hospital,the demand for pharmacy intravenous admixture services (PIVAS) has been gradually increased.By being built inside the pharmacies,PIVAS of this hospital has created a full range of integrated pharmacy service platform.With independent research and development of information management system,innovative design of the modules and equipments based on the concept of internet of things,and integration of artificial and computerized checking,PIVAS of this hospital has formed a system of prevention and control chain,it has been deepening the connotation of clinical pharmacy services,and providing higher quality pharmacy services to clinical drug using.

Humans ; Integrative Medicine ; methods ; Medicine, Chinese Traditional ; methods ; Neoplasms ; prevention & control ; therapy ; Quality Control ; Research Design

Objective To observe the clinical efficacy of auricular point sticking plus moxibustion at Zusanli (ST 36) in preventing spleen-stomach disharmony due to intravenous analgesia pump.Method Totally 120 post-operation patients with intravenous analgesia pump were randomized into a treatment group and a control group, 60 in each group. Patients in the control group were asked to take a semireclining position, and to turn over the body and do morning exercise under assistance. In addition to the intervention given to the control group, the treatment group received auricular point sticking plus moxibustion at Zusanli (ST 36). The occurrence rate of post-operation nausea, vomiting and abdominal bloating was observed.Result The occurrence rate of nausea, vomiting and abdominal bloating was 11.7% in the treatment group, versus 33.3% in the control group, and the difference was statistically significant (P<0.05).Conclusion Auricular point sticking plus moxibustion at Zusanli (ST 36) is effective in preventing spleen-stomach disharmony due to intravenous analgesia pump, and can reduce pain and complications.

Inflammasomes are multi-protein complexes that trigger the activation of caspase-1 and the maturation of interleukin-1β ( IL-1β) , which are critical for inflammation and control of pathogen infection. Inflammasomes are involved in and regulate diversified diseases due to its capability to sense multiple danger signals. In this review, we present the activation and regulation mechanisms for different inflammasomes and discuss how genetic mutations in inflammasome-related genes or abnormal activity of in-flammasomes lead to diseases. Insights into the role of inflammasome in various human diseases will provide sound theoretical basis and effective treatment strategy for these disease.

Objective To evaluate the effect of nasal continuous positive airway pressure (nCPAP) in pediatric patients with respiratory failure after liver transplantation.Method A prospective? randomized controlled clinical trial was conducted during June 1st 2013 to June lst2015 in 71 pediatric patients with respiratory failure after liver transplantation.A total of 66 patients completed the trial and 5 patients quitted.Conventional oxygen therapy group included 35 cases and nCPAP group included 31 cases.The vital sign,blood-gas analysis,intra-abdominal pressure and prognosis were compared between the two groups.Result After nCPAP treatment,the heart rate,respiratory rate,and PCO2 decreased at 4 and 24 h (P＜0.05),oxygenation index was improved at 24 and 48 h (P＜0.05),and intra-abdominal pressure decreased at 48 h (P＜0.05).nCPAP showed better outcome than conventional oxygen therapy (P＜0.05).The incidence of refractory atelectasis,intra-abdominal hypertension,and epilepsy was higher in treatment failure population.Conclusion nCPAP is more suitable for pediatric patients with respiratory failure after liver transplantation,especially for those accompanied with intra-abdominal hypertension.

Objective To construct pEGFP-N1-TGF-β1 recombinant plasmid and transfect it into primary cultured neonatal piglet type Ⅱ alveolar epithelium cell (AEC-Ⅱ) by using lipofectamine 2000,in order to provide basis of methodology for producing recombinant plasmids for transplantation of transfected AEC-Ⅱ into ALI/ARDS animal model lungs.Methods PCR primers were designed to amplify the human TGF-β1 cDNA fragment from plasmid.XhoI and EcoRI were used for double digesting the empty plasmid pEGFP-N1 and cDNA fragment of human TGF-β1.Then the products of double enzyme digestion by using T4 DNA ligase were connected and transformed into DH5α and cultured over night for 16 hours.The structure of recombinant plasmid was identified by using PCR and base sequencing to verify the correctness of pEGFP-N1-TGF-β1 recombinant plasmid.It was then transfected into primarily cultured AEC-Ⅱ by lipofectamine2000 mediated transfection and cultured for another 48 hour.Plasmid DNA (pEGFP-N1-TGF-β1 recombinant plasmid) and lipofectamine 2000 were added into serum-free DMEM respectively,then DNA suspension and Lipofectamine 2000 suspension were blended together and added into cells.After 24-48 hours later,the expression level of enhanced green fluorescent protein (EGFP) was evaluated under fluorescence microscope.Results The structure of vector was verified as pEGFP-N1-TGF-β1 recombinant plasmid by using PCR and base sequencing.Green fluorescence found in some cells showed that the pEGFP-N1-TGF-β1 recombinant plasmids had been successfully transfected into primary cultured AEC-Ⅱ,however,the transfection efficiency still need tobe further improved such as repeating the transfection procedure once again or using adenovirus mediated transfection method to improve the efficiceny and to transplant the cells into animal lungs eventually.Conclusions pEGFP-N1-TGF-β1 recombinant plasmid was successfully constructed and,for the first time,transfected into primarily cultured AEC-Ⅱ of newborn piglets.This established method should be useful for investigation of therapeutic effect and outcomes of lung with experimental acute lung injury.