[Objective]To compare clinical results of the mini midvastus approach with the traditional medial parapatellar approach for totall knee arthroplasty and to evaluate their advantages.[Methods]Sixty total knee arthroplasty(56 patients)have been freated during May 2002 to May 2006,28 patients(30 knee) were underwent TKA using either mini midvastus approach or traditional medial parapatellar approach.Two groups were compared according to skin incision length,postoperative pain score,total amount of drains,postoperative amount of decreasing Hb,active straight-leg raise time,postoperative length of stay,radiographic alignment of all the components,postoperative range of motion at 6 weeks and 12 weeks and 1 year,postoperative HSS score at 1 year.[Results]All the patients were followed from 1 to 1.5 year(mean,13.2 months).Position of all the components was normal in all patients except one case with abnormal position of tibial prosthesis in both groups.In the MIS group,the average skin incision length was 11.3 cm,average visual analog pain scale was 2.76 score,while the control group was 20.6 cm and 3.8 score.In the MIS group,the total amount of drains was 96.8 ml,postoperative amount of decreasing Hb was 22.5 g,while the control group was 276.3 ml and 32.5 g.In the MIS group,active straight-leg raise time was 3.8 days,postoperative length of stay was 8.6 days,while the control group was 5.8 days and 12.1 days.In the MIS group,the mean ROM at 6 weeks postoperatively was 107?and 117?at 12 weeks,while the control group was 98?and 108?.The differences between each factor group and operative results were significant(P0.05).[Conclusion]The mini midvastus approach wis associated with a more rapid functional recovery and a more satisfactory short-term result.

OBJECTIVE:To work out the optimal inventory levels in zero inventory management mode through model predic-tion and control strategy,by using the inventory upper & lower limits settings generally available in the information management system of health care institutions. METHODS:Multi-varieties joint ordering modelwas constructed by referring to operations management,time series analysis and quantitative approach to decision-making,that is,to make a prediction of upper&lower lim-its on medicine inventory based on historical data and applicable mathematical models(fixed order interval model and re-order mod-el,i.e. FOI and ROP),and compared with real results;based on above,specific medicine procurement and inventory control strat-egies would be developed and an evaluation of the application effects would be made. RESULTS:The error test and reproducibility test exhibited that the out-of-stock ratio remained under 3.36%,of which 71.24% could be effectively alarmed;under computer simulation and practical operation,the instant replenishment rate reduced by 9.33% and 13.03%,OOS ratio down by 11.11% and 27.45%,and average daily inventory turnover rate up by 30.19% and 15.85% respectively,all showing remarkable improvements compared to before the implementation of the mode. CONCLUSIONS:This model is of favorable accuracy and operability,there-fore it can lay foundation for rational and well-founded decisions in medicine procurement and inventory control in zero inventory management mode.

Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.

Objective To investigate the role of CT three-dimensional reconstruction (3D-CT) in the diagnosis of bronchial foreign body.Methods Thirty-seven suspected pediatric cases of bronchial foreign bodies underwent CT scanning and 3D-CT reconstruction,whose accuracy was defined by bronchoscopical or surgical findings.Results For all the 37 cases,chest X-ray showed 8 cases (group A) had bronchial foreign body,while 29 cases(group B) were negative.In group A,CT findings suggested lung infection in 5 cases,congenital pulmonary dysplasia-lung lobe absence in 2 cases and tracheo-esophageal fistula in 1 case.In group B (29 patients),25 cases with bronchial foreign bodies were suspected by CT scanning and further bronchoscopical examination confirmed the existence of 24 cases of vegetable foreign body,one case of the tracheal tumor.For the four cases with negative result of bronchial foreign bodies after CT scanning,lung infection and laryngitis were confirmed in two cases respectively.Among the 24 surgically-confirmed cases of vegetable foreign bodies,CT scanning showed tracheobronchial foreign body in fourteen positive results,irregular tracheal stenosis in six cases and obstructive atelectasis,emphysema,pneumonia in seven each.Tracheobronchial foreign body and irregular tracheal stenosis were direct signs,accounting for 74%(20/27).Obstructive atelectasis,emphysema and pneumonia were indirect signs,accounting for 26%(7/27).Conclusion 3D-CT plays an important role in diagnosis and differential diagnosis of bronchial foreign body.

BackgroundCorneal graft reject is a major cause of corneal transplantation failure.Although many immune-suppressing drugs have been utilized to reduce the reject response,their adverse effects on organ and tissue are still insoluble.The tolerance induction of natural killer T (NKT) cells is currently under investigation.However,the study on the application of NKT cells in high risk corneal transplantation is seldom.ObjectiveThe present study was to explore the effects of α-GalCer-activated NKT cella on allografts survival after high-risk corneal transplantation surgery via retro-bubar injection.Methods The lymphocytes were picked up from the spleen of SPF Lewis rats and cultured in RPMI 1640 medium with 100 mg/L α-GalCer.After one week,NKT cells were sorted by the FACSVantage system as CD161+ TCR-α+ cell from the lymphocytes with the cell densities 5×106/ml.Ten SPF Fisher344 rats were used to prepare the donor corneas,and 20 Lewis rats served as recipients.The high risk corneal transplantation models were created by corneal suturing in 20 recipient rats.Penetrating keratoplasty (PKP) was performed in the model rats.0.1 ml NKT cells or the same volume of normal saline solution were retro-bubarly injected at the end of surgery respectively.The corneal allografts were observed and scored based on Holland criteria at the three-day interval under the slit lamp for 30 days.Two weeks after surgery,three rats from each group were sacrificed by excessive anesthesia method and the eyeballs were obtained for histopathological examination.The inflammatory cell infiltration ( CD4+ and CD8+ ) in grafts was evaluated by immunochemistry and flow cytometry.The use of the animals complied with the Statement of ARVO.ResultsThe mean survival time of the allografts was (7.90± 1.37) days in normal saline solution group and (14.70± 1.49) days in NKT cell group,showing a statistically significant difference between the two groups ( t =10.61,P =0.00 ).Two weeks after surgery,all the allografts showed the severe opacity with lots of new blood vessels and edema in normal saline solution group.However,the corneal grafts were clear in NKT cell group.Abundant CD4+ and CD8+T lymphocytes were seen in the allografts in normal saline solution group,but the inflammatory cells were obviously less in NKT cell group.The percentage of NKT cells in the spleen was (5.67±0.25)％ in NKT cell group and ( 1.21±0.19)％ in normal saline solution group ( t =8.43,P =0.00 ).Conclusionsα-GalCer-activated NKT cells can prolong the survival time of allografts in high-risk corneal transplantation.Retro-bubar injection of α-GalCer-activated NKT cells probably is a new approach to the prevention of the rejection of corneal transplantation.

After a description of medical literature retrieval teaching in our library, the contents in the designed medical literature retrieval practice course teaching and the ideas for its reform, the competitive practice method was proposed according to the characteristics of subjects in which the undergraduates were majored and the experi-ence with several teaching models such as PBL, the effect of its implementation was analyzed with its related prob-lems and measures for their solution pointed out.

60 Chinese skulls(30 males and 30 females) from Liaoning province of the People's Republic of China were measured, By applying the multiplestepwise discriminant function, sexual diagnosis of the maxillary, frontal, occipital and parietal bone were carried out, 12 discriminant equation for sexual diagnosis have been obtained,The diseriminant rate of equations with accuracy tests on the same series of crania results in 66.7～93.3% of the cases analyzcd

Objective:To analyse the property of voltage-dependent potassium 〔K(v)〕 channel in healthy people′s peripheral lymphocyte so as to contribute the control for property alteration under some pathological condition,and to try to find the new subset of this channel.Methods:Patch-clamp whole cell recording technique was used.Results:In the recorded 39 cells,activated voltage of the channels was -40.3±2.5 Mv.No inactivation phenomenon appeared under repeated stimulation.The closing time of the channels was 116.3+8.2 ms under the repolarization,and the current could be inhibited by 10 mmol/L TEA.Conclusion:There might be only one type of K(v) channel in human peripheral blood lymphocytes,and its properties quite resumble the type of n K(v) channel in mice.

Objective To investigate the relationship and mechanism between the serum level of high mobility group box protein-1(HMGB1)and the mortality rate in patients with sepsis.Methods The serum levels of HMGB1,superoxide dismutase(SOD)and malondialdehyde(MDA)in 48 patients with sepsis were determined.The clinical outcomes in those patients were recorded and anlyzed.Results After the onset of sepsis,the serum HMGB1 levels of both death group and survival group were increased gradually and peaked at 72h after the onset of the disease.The semm HMGB1 levels of death group were much higher than those of survival group except at 24h(t=6.07,6.20,24.43,all P＜0.05).The activity of serum SOD of death group was markedly lower than that of survival group at 12h,24h,48h and 72h(t=10.24,20.61,11.67,33.33,all P＜0.05),and the level of serum MDA of death group was significantly higher than those of survival group at all time points(t=26.06,22.17,23.86,9.49,5.95,all P＜0.05).There was a significantly positive correlation between the serum HMGB1 and MDA level.Conlusioa The increase in serum HMGB1 level may be the important reasoll for the increased mortality rate in patients with sepsis;Oxidant/antioxidant imbalance may be olle reason for the increase in serum HMGB1 level.

Objective:To investigate the relationship between “activation of IL 6 signal transduction pathways” and “induction of CD45 expression” in a myeloma cell line—Sko 007 by IL 6.Methods:Electrophoretic mobility shift assay(EMSA) was used to detect the activation of the transcription facotrs STAT3 and NF IL 6 by IL 6,which can represent the activation state of the two IL 6 signal transduction pathways respectively;Then the induction of CD45 mRNA and protein expression by IL 6 were detected by RT PCR and FACS.The effect of antisense expression plasmid of STAT3 pAT STAT3 on STAT3 activation and CD45 expression was shown by EMSA and RT PCR,respectively.Results:①Both two IL 6 signal transduction pathways JAK/STAT and Ras/NF IL 6 can be activated by IL 6 in Sko 007 cells.②The expression level of CD45 mRNA and protein is Sko 007 cells can be up regulated by IL 6.③The activation of JAK/STAT signal transduction pathway and induction of CD45 mRNA expression by IL 6 can be inhibited in pAT STAT3 transfected Sko 007 cells.Conclusion:IL 6 induced CD45 expression in Sko 007 cells in mediated by the activation of JAK/STAT signal transduction pathway.