Objective To explore the value of P_ 300 in early diagnosis of vascular dementia (VD) and possibility of P_ 300 in the differential diagnosis between VD and Alzheimer's disease (AD).Methods Hasegawa Dementia Scale (HDS) and P_ 300 of oddball auditory evoked model were performed in 20 normal controls,20 patients with VD,24 patients with cerebral infarction without dementia (CI) and 20 patients with AD.Results The peak latency (PL) of target N_2 and P_3 in VD group was obviously longer than those in CI and NC groups,the difference was dramatic(all P

Humans ; Infant, Newborn ; Syphilis Serodiagnosis ; Syphilis, Congenital ; diagnosis

[Objective]To investigate the clinical effects of the internal fixation for fracture and dislocation of cervical spine with pedicle screws.[Method]Forty-one cases suffered from cervical fracture and dislocation were treated with Axis system.The patients all took X-ray,CT and MRI examinations before operations.Personal manipulation of every pedicle screw was attained according to the imaging measurement.[Result]All of the patients were followed up for six to twelve months postoperatively.One hundred and ninty-six of total 218 screws (90%) were in correct positon and 22 were incorrect.One nerve root injury and one doubtful blood vessel injury happened.Complete reduction and fracture union were obtained in 38 cases while inadequacy reduction and nerve root irritation existed in 3 cases,one of which suffered anterior approach operation on account of degeneration.Recovery didn't happen in the 6cases with complete spine injury.Ninteen cases with inadequacy spine injury recovered apparently even near normal level.[Conclusion]Cervical pedicle screw technique was identified to be safe and feasible for cervical fracture and dislocation.The key factors for a successful operation contain selection of patients,familiarity of the cervical anatomy,standard skill of manipulation and individuality of screw placement.

To investigate the clinical significance of the expression of CD40L on peripheral blood monocytes and the changes in serum soluble CD40L in patients with acute coronary syndrome, 16 normal controls and fifty-six patients including 24 with SA (8 patients after PTCA), 20 with UA and 12 with AMI entered in this study. The expression of CD40L on monocytes was analyzed by indirect immonofluorescence flow cytometry and serum sCD40L levels were measured by ELISA. The results showed: (1) The expression of CD40L on monocytes in UA and AMI were higher compared with SA and controls ( P 0.05). (2) Patients with UA and AMI had significantly raised serum levels of sCD40L when compared with patients with SA and controls( P

Objective To investigate the feasibility and safety of transabdominal perposterior mediastinum radical operation in the treatment of cardiac carcinoma. Methods Twenty-six cases of cardiac carcinoma invading low esophagus underwent transabdominal posterior mediastinum radical resection. Among them 9 patients received radical proximal gastroesophagectomy, and 17 cases did radical total gastroesophagectomy. Results The procedures were all successful. The mean operation time for total gastrectomy was (189±39) min, proximal subtotal gastrectomy was (153±35) min. The averge blood loss of total gastrectomy was (200±80) ml, proximal subtotal gastrectomy was (168±76) ml. The harvest of celiac lymph nodes were (23.3±7.3), esophageal lymph nodes were (4.1±2.0). Pneumonia was complicated in 4 cases and there was no anastomotic hemorrhage, leakage nor stenosis. All patients were followed up from 5 to 51 months, liver metastasis was found in 4 cases, lung metastasis in 2 patients and death in 1. There was no anastomotic stoma tumor recurrence. Conclusion It was feasible and safe to perform transabdominal perposterior mediastinum radical operation for cardiac carcinoma patients, provided that diaphragms was not involved and tumor invaded esophageal length was less than 5 cm.

Objective To investigate the inhibitory effect of cryptotanshinone on different maturation stages of Staphylococcus epidermidis (S.epidermidis) biofilm.Methods The biofilm model of S.epidermidis was constructed in vitro, the timing of adhesion, accumulating, and maturation was determined;matrix quantity, bacterial metabolism, microstructure of biofilm were detected with semi-quantitative adhesion test, XTT assay, and scanning electron microscope(SEM) respectively.Results The timing of adhesion, accumulating, and maturation of S.epidermidis biofilm were 6h, 24h,and 48h respectively;in adhesion period, cryptotanshinone at the concentration of 128μg/mL and 32μg/mL could both obviously reduce the matrix and kill bacteria inside biofilm, difference was statistically significant(P<0.05),inhibitory effect of 128μg/mL cryptotanshinone was better than 32μg/mL (P<0.05), the microstructure was destroyed by both concentrations.During accumulating and mature period, only cryptotanshinone at 128μg/mL could reduce the matrix of biofilm and kill bacteria inside biofilm (P<0.05), the microstructure was damaged by cryptotanshinone at concentration of 128μg/mL, while 32g/mL of cryptotanshinone had no obvious inhibitory effect(P>0.05).Conclusion Cryptotanshinone has a certain inhibitory effect on different stages of S.epidermidis biofilm, and there is a certain dose effect.

Objective To understand the distribution and drug resistance of pathogens causing catheter related bloodstream in‐fection (CRBSI) to provide reference for clinical treatment .Methods The distribution and drug resistance of pathogens isolated from the central venous catheter from January 2011 to June 2015 were retrospectively analyzed .Results Among 731 submitted samples ,38 cases were CRBSI ,with the positive rate of 5 .3% ,in which ,the Gram‐positive cocci accouted for 26 .3% of isolated bacteria and dominated by Staphylococcus epidermidis (13 .2% ) ,moreover which was MRSE .MRSA and VRE were not detected . Gram‐negative bacilli accounted for 73 .7% of isolated bacteria and dominated by Acinetobacter baumannii (42 .1% ) ,which was most sensitive to amikacin with the sensitivity rate of 87 .5% .Conclusion Acinetobacter baumannii is most common pathogen in CRBSI with serious drug resistance ,therefore the operating should be standardized in clinical work for controlling infection .

Objective To investigate the protective effect of pretreatment with Shenfu injectio(SFI)on myocardium against ischemia-reperfusion(I/R)injury in diabetic rabbits.Methods Forty healthy adult male rabbits weighing 2.4-3.2 kg were used in this study.Type I diabetes mellitus was induced by intravenous alloxan 120 mg?kg~(-1) and confirmed by fasting blood glucose＞11.1 mmol?L~(-1).The animals were randomly divided into 5 groups(n=8 each):groupⅠsham operation;groupⅡI/R and groupⅢ,Ⅳ,ⅤSFI+I/R.I/R was produced by occlusion of the anterior descending branch of left coronary artery(LAD)for 60 min.The occlusion of LAD was then released for reperfusion.In sham operation group(Ⅰ)LAD was exposed but not occluded.In groupⅢ,ⅣandⅤSFI 5,10 and 15 ml?kg~(-1) was givenⅣrespectively 30 min before myocardial ischemia.MAP,HR,left ventricular developed pressure(LVDP),?dp/dt_(max),left ventricular end-diastolic pressure(LVEDP)and Vmax were recorded immediately before ischemia(T_0,baseline),at 5 min of ischemia(T_1),immediately before reperfusion(T_2)and at 5,15,60,90,120 min of reperfusion(T_(3-7)).The animals were killed and hearts removed for determination of infarct size and microscopic examination of the ultrastructure of LAD using transmission electron microscope.Results MAP,HR,LVDP,?dp/dt_(max) and Vmax were significantly decreased while LVEDP was significantly increased during reperfusion as compared to the baseline values at T_0 in I/R group. I/R produced myocardial infarct and damage to the endothelial cells of LAD.The harmful effect of I/R was attenuated by different doses of SFI.SFI 10 ml?kg~(-1) provided best effect.Conclusion Pretreatment with SFI can protect the myocardium against I/R injury in diabetic rabbits.SFI 10 ml?kg~(-1) produces better effect.

In this article, two research cases are employed to show the rationale of the stepped-wedge design, under what situations that such a design is desirable, and how it can be implemented. Stepped-wedge design seems to suit to randomized controlled trials in which the entire study population will receive intervention programs as they would "provide more advantages than harm". When intervention can not be given to all the targets simultaneously due to limited resources, this design is particularly useful. The stepped-wedge design is also relevant when there is a hope to detect or control the time trend effect on the effectiveness of the intervention strategy. On the other hand, however, this design requires longer trial duration and presents a number of statistical challenges. Hence, careful planning and monitoring are essential to ensure that a robust evaluation is undertaken.

Objective:To evaluate the efficacy and safety of tirofiban,a platelet glycoproteinⅡb/Ⅲa Inhibitor,in percutaneous coronary intervention(PCI)of patients with acute non-ST segment elevation myocardial infarct(NSTEMI).Methods:A total of 114 patients with acute NSTEMI were enrolled in the trial from Sep.2005 to Jan.2007;they were randomly divided into 2 groups:tirofiban group(n=57)and placebo group(n=57).Patients in tirofiban group were given tirofiban for 24 h after PCI.All patients were routinely given heparin,aspirin and clopidogrel before CPI.The composite occurrence of death,myocardial infarction(MI),need for target vessel revascularization(TVR)after PCI,and the adverse effects(hemorrhage and thrombocypenia)were compared between the 2 groups.Results:One(1.8%)patient had angina pectoris and the other(1.8%)developed subacute thrombus in control group within 24 h after PCl;there was no such event in the tirofiban group.Two(3.6%)patients developed angina pectoris and 2(3.6%) developed subacute thrombus within 30 days after PCI in control group;one patient(1.8%)in birofiban group developed angina pectoris and one patient in birofiban group developed subacute thrombus.Each group had one case(1.8%)of upper digestive tract bleeding during hospitalization.No intracranial hemorrhage,skin/ mucosa hemorrhage,thrombocytopenia,or-death occurred in the 2 groups.Intravenous tirofiban treatment reduced the composite occurrence of death of NSTEMI patients after PCI(P