Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Objective To investigate the effect of Fructus Ligustri Lucidi(FLL),a kidney-tonifying Chinese herbal medicine,on calcium metabolism and vitamin D-dependent gene expression in ovariectomized(OVX) rats.Methods Four weeks after OVX surgical operation the OVX rats were ig admini-(stered) with estradiol and FLL for 14 weeks.Ca~(2+) Level in serum,urine,and feces was measured by colorimetric methods,and gene expressions in duodenum and kidney were analyzed by reversed transcriptase-polymerase chain reaction(RT-PCR).Results Ovariectomy of rat led to significant loss of calcium in vivo,as demonstrated by decreasing serum Ca~(2+) level and increasing Ca~(2+) excretion rate in urine and feces in OVX rat group.Treatment of OVX rats with FLL could effectively restore serum Ca~(2+) level and prevent the increase of Ca~(2+) excretion from urine and feces.RT-PCR Analysis showed that both estradiol and FLL could prevent OVX-induced decrease of mRNA expression in duodenal vitamin D receptor(VDR).In kidney,FLL did not alter mRNA expressions of 25-hydroxyvitamin D 1-hydroxylase(1-OHase) and 25-hydroxyvitamin D 24-hydroxylase(24-OHase),while increased the mRNA expression levels of renal cal-ciumbinding protein-9k(CaBP-9k) and calcium-binding protein-28k(CaBP-28k).Conclusion FLL could improve the state of calcium imbalance induced by estrogen deficiency and the potential mechanism that contributes to the improvement of calcium status by FLL might involve the increase in intestinal sensitivity to active vitamin D through its up-regulation of VDR expression in duodenum and the enhancement in Ca~(2+) reabsorption via the up-regulation of calcium binding proteins expression in kidney.

Objective:To explore the opportunity and the manner of termination of pregnancy.Methods:The data of 200 cases of ICPfrom January 2007 to June 2008 were retrospectively analyzed.Maternal pruritis,liver function ,glycocholic acid(CG),amniotic fluid condition, the time and the way of termination of pregnancy,Apgar score were examined.Three groups were classified by the values of CG:Group A (CG

Objective:To observe the effects of radix astragali on hypertrophic scars in rabbit ears. Methods:Twenty healthy adult Japanese big ear rabbits were given hypertrophic scars over the ventral surface of the ears and on the 21st day after wound,animals were randomly divided into the treated groups (groups A,B,and C were treated with radix astragali in different concentrations)and the control groups(groups D and E were treated with saline and blank control,respectively). On the 32nd and 43rd day after wound,the thickness and hardness of scar were measured.The TGF-?1mRNA were detected in hypertrophic scar samples with RT- PCR.The change of scar tissues was observed with H&E staining and immunohistochemical staining with TGF-?1. Results:The thickness and hardness of scar were inversely correlated with the concentration of radix astragali in the radix astragali-treated group(P0.05)between the 32nd day and43rd day groups.Conclusion:Radix astragali could inhibit proliferation of hypertrophic scars in the rabbit ears by decreasing the expression of TGF-?1mRNA and its protein. Thereby radix astragali may have beneficial effects in the treatment of hypertrophic scar.

0.05).Conclusion:The results support our hypothesis that APS can be used to treat the hematopoietic type of acute radiation sickness.APS pretreated groups had better results than APS treated groups in initial observation.

Objective:To observe the effect of intrapulpal injection in the pulpectomy of primary molars.Methods:22 petients with 44 diseased primary molars were divided into 2 groups(n =22 teeth).The intrapulpal injection with a computer-controlled local anesthetic delivery system STATM was performed in the experimental Group,and traditional infiltration anesthesia technique was conducted in the control group.Results:During the anesthesia process,the VAS value of the experimental group was lower than the control group(P ＜ 0.05).The pain control in the experimental group was better during pulp removal within 1 min.There was no occurrence of postoperative soft tissue numbness and factitial ulcer in the experinental group.Conclusion:The intrapulpal injection,administered with STATM,is more effective than traditional infiltration anesthesia in pulpectomy of primary molar teeth.

As an available fault-tolerant technique of ORACLE,standby database can be used to perform disaster recovery.This article describes how to create and maintain an ORACLE standby database,and provides the solutions of common problems.

Objective:To study the application value of Belinson biopsy forceps and traditional biopsy forceps in cervical intraepithelial neoplasia. Methods:Two hundred and fourty cases of cervical intraepithelial neoplasia patients received cervical biopsy under colposcopy from 2011 August to 2014 March were enrolled and randomly divided into observation group received Belinson biopsy forceps biopsy and control group received conventional biopsy forcep. Then coincidence rate between cervical biopsy and final pathological diagnosis, biopsy condition and stress levels of two groups were compared. Results:Coincidence rate between cervical biopsy and final pathological diagnosis of observation group was higher than that of control group;biopsy tissue volume, vaginal bleeding amount were less than those of the control group; pain scores after biopsy was lower than control group and cervical repair time was shorter than control group. Serum ACTH, Cor, NE levels were lower than control group. Conclusion:Belinson biopsy forceps biopsy is helpful to improve the accuracy of diagnosis, control the degree of tissue trauma and stress reaction degree, promote cervical repair and reduce local bleeding after biopsy;it’s an ideal method in biopsy of cervical intraepithelial neoplasia.