Objective:To investigate the effects of sodium butyrate,the most important short chain fatty acids,on growth suppression and apoptosis of SHG-44 glioma cell line.Methods:Human glioma SHG-44 cells were treated with 2 mmol/L and 4 mmol/L sodium butyrate.The inhibition of cell growth was assessed by MTT spectropotometric analysis and immunohistochemisty methods.The apoptosis was observed by reverted microscope and transmission electron microscope.And mitochondrial transmembrane potential was detected by rhodamine 123 dyeing,which confirmed that SHG-44 cells suffered a mitochondrial path apoptosis.Results:Sodium butyrate treatment resulted in significant inhibition of cell growth,with proliferating cell nuclear antigen expression weakened.Furthermore,4 mmol/L sodium butyrate could induce the SHG-44 cells apoptosis.It was proved that cellular membrane shrunk,vacuoles existed in plasm,and apoptosis bodies occurred by morphologic observation.In addition,the △?m was decreased in apoptosis cells after 4 mmol/L sodium butyrate treatment.Conclusion:These results indicate,in malignant glioma SHG-44 cells,sodium butyrate could not only suppress cell growth,but also induce apoptosis by a mitochondrial path.

In view of the increased incidences of cardiac arrest outside hospital year by year and younger tendency of the patients,foreign medical education practice showed that school-age children accepting cardiopulmonary resuscitation (CPR) skill training will benefit most from this survival skill in the future.This article elaborated on the meaning and methods of promoting CPR training in primary and middle school based on the present situation of CPR in order to promote its further development.

Humans ; Practice Guidelines as Topic ; Research Design

Meta-Analysis as Topic

Objective To investigate the effect of sodium butyrate on the growth suppression and cellular differentiation of cultured SHG 44 glioma cell line Methods Human glioma SHG 44 cells were treated with 2 mmol/L sodium butyrate The inhibition of cell growth was assessed by MTT spectrophotometric analysis and AgNORs analysis The cellular differentiation was determined by flow cytometry, electron microscopy, immunohistochemical method and agglutination test Results Treatment of SHG 44 cells with sodium butyrate resulted in significant inhibition of cell growth and G1 S transition in time dependent and dose dependent manners Sodium butyrate could induce differentiation of SHG 44 cells with regularized nuclei and increased heterochromatins The mitochondria and rough endoplasmic reticulum increased in density in normal state Furthermore, SHG 44 cells expressed more GFAP but less vimentin, and the agglutination degree decreased obviously Conclusion Sodium butyrate may suppress glioma cells growth and induce differentiation of human glioma cells

Animals ; Evidence-Based Medicine ; Health Knowledge, Attitudes, Practice ; Humans ; Infant ; Meta-Analysis as Topic ; Milk Hypersensitivity ; diagnosis ; prevention & control ; Milk Proteins ; adverse effects ; Practice Guidelines as Topic ; standards ; Professional Staff Committees ; organization & administration

ObjectiveTo survey the social support to students in university.Methods400 college students of grade 2004 from 4 different colleges in Ningbo were tested with the Social Support Rating Scale(SSRS).Results and ConclusionThere were no significant differences among students from Ningbo and students from the other regions.Students from different level colleges had different scores in utilization of support,students from the third level college had the highest scores while students from the forth level had the lowest scores.College students from countries scored higher than students from cities in subjective support,but there were no significant differences in objective support and the utilization of support.Female students had higher scores in subjective support and utilization of support than males while had no significant differences in objective support.Students with different majors had no significant differences in objective support and utilization of support,while concerning subjective support,students from management departments had the highest scores,students from arts departments had the lowest scores.In case of emergency,college students mainly received economic support and mental support from their families,friends and classmates while received less support from various organizations both of colleges and society.

ObjectiveTo investigate the relationship between family cohesion, family adaptability and perceived social support.Methods400 college students of grade 2004 from 4 different colleges of Ningbo were tested with the FACESⅡ-CV and perceived social support scale. Results and ConclusionFamily cohesion and adaptability significantly positively correlated with perceived family support and social support. There was no significant correlation between perceived family support and dissatisfaction with family cohesion, while perceived family support significantly negatively correlated with family adaptability. In family adaptability, dissatisfaction with family cohesion and family adaptability, there were significant differences among students from different level colleges. In perceived family support, there were no significant differences among students from different college. In family cohesion, female students scored higher than male, but showed no significant differences in family adaptability. In perceived family support and social support, female students had higher scores than male were. In family cohesion and perceived family support, fathers' education background but not mothers' had significant main effect. In family cohesion, family adaptability and perceived family and social support, there were no significant differences between students from cities or countries.

Chronic cerebral hypoperfusion is a common pathological state. Cognitive impairment is its main manifestation in early stage, and it will eventually result in persistent or progressive cognitive impairment and neurological deficits. Chronic cerebral hypoperfusion caused neuronal damage, synapse abnormity, energy impairment and the function defects of central cholinergic system and monoaminergic system are its pathophysiological mechanisms resulting in cognitive impairment.

Objective To explore the effect of intervention on depression and negative coping in elderly patients with chronic disease and provide a basis for mental health services. Methods Forty depression and negative coping of elderly patients with chronic disease were took psychological intervention for one year. Geriatric depression scale (GDS) and ways of coping scale (WCS) were used to assess the intervention effect. Results There was a high scores of GDS before the study (20.93?1.90)and the scores were reduced after intervention in Six months and the end of one year (17.88?1.80,11.80?1.60,respectively,P0.05). After intervention for one year,the patients applied more with positive coping styles such as problem sloving,help seeking and less with wince,illusion and self-criminating,there was a statistically significant difference (P