Cerebral Cortex ; growth & development ; physiology ; Evoked Potentials, Visual ; physiology ; Humans ; Infant, Newborn ; Infant, Premature ; growth & development ; Sensory Thresholds ; physiology ; Vision, Ocular ; physiology ; Visual Acuity ; physiology ; Visual Perception ; physiology

Abstract?AlM: To study the effect of latanoprost combined with timolol treatment on visual function, intraocular pressure and ocular blood flow of open angle glaucoma.?METHODS:A total of 50 cases ( 59 eyes ) with open angle glaucoma were enrolled from January 2012 to May 2014 in our hospital department of ophthalmology, and divided into observation group and control group by adopting the random number table method. Patients in observation group were treated with latanoprost combined with timolol, patients in control group were treated only with timolol. Visual function, intraocular pressure and ocular blood flow were compared.?RESULTS:1, 2, 3 and 4wk after treatment, observation group during the day intraocular pressure, night intraocular pressure were significantly lower than that in control group, vision levels (0. 27±0. 03, 0. 36±0. 06, 0. 44± 0. 06, 0. 63 ± 0. 13 ) were significantly higher than that in control group; observation group peak systolic velocity (14. 41± 1. 73) cm/s, end diastolic velocity (4. 18 ± 0. 67) cm/s were significantly higher than that in control group;vascular resistance index ( 0. 58 ± 0. 07 ) was significantly lower than that in control group.? CONCLUSlON: Latanoprost combined with timolol treatment can reduce intraocular pressure, increase blood flow of central retinal artery, reduce vascular resistance, improve visual acuity.

Objective To get the information about the infection condition of blood in voluntary donors in Fuling District from 2001 to 2007, so as to take measures to improve blood quality and ensure blood safety.Methods Serum ALT was detected with Reitman-Frankel assay, and HBsAg, anti-HCV, anti-HIV and anti-TP were detected with ELISA.Results A total of 72 868 serum samples from blood donors were detected, in which 5 512 (7.56%) were positive. The positive rates of HBsAg, anti-HCV, anti-HIV and anti-TP were 3.09%, 0.48%, 0.07% and 1.27% respectively, and the abnormality rate of ALT was 2.73%. There was statistical difference of positive rate among various years (from 2001 to 2007).Conclusion The positive rate of blood in voluntary donors decreases year by year. HBsAg was the highest (1.16%), followed by ALT (2.73%), anti-TP (1.27%), anti-HCV and anti-HIV. It suggests that blood quality monitoring should be strengthed to assure blood safety.

Objective To study the effect of rosiglitazone (RSG), the agonist of peroxisome proliferator activated receptor γ (PPARγ), on the proliferation and differentiation of rat osteoblasts and the related mechanisms. Methods The identification of rat primary osteoblasts was performed by alkaline phosphatase (ALP) staining and mineralized nodules. The 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay and p-nitrophenyl phosphate (PNPP) assay were used to observe the effects of different concentrations of RSG on proliferation and differentiation of the osteoblasts. The reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of connective tissue growth factor (CTGF) mRNA. The effects of the different concentrations (0,1,2,5,10 and 20 μmol/L) of RSG on TGF-β1-induced CTGF mRNA expression in osteoblasts were detected. Results (1)Different concentrations of RSG could not change the proliferation of osteoblasts (P＞0. 05). (2)Compared with control group, all different concentrations of RSG could suppress ALP activity in osteoblasts (P＜0. 01 ). (3) RSG suppressed the osteoblats CTGF mRNA expression induced by TGF-β1 in a dose-dependent manner (P＜0. 01). Conclusions In vitro, RSG can inhibit the TGF-β1 induced rat osteoblasts CTGF mRNA expression. RSG may play a potential role in preventing the differentiation of the rat osteoblasts.

Hypertension is the most important risk factor for ischemic stroke and other cardiocerebrovascular diseases.However,the mean blood pressure can not completely explain the target organ damage caused by hypertension and the benefits of antihypertensive treatment.A series of studies have demonstrated that blood pressure variability (BPV) is the risk factor for ischemic stroke independent of the mean blood pressure,and there was also a significant correlation between BPV and ischemic stroke.In recent years,the research of BPV has gradually become a hot spot in the field of prevention and treatment of ischemic stroke.However,the specific relevance between BPV and ischemic stroke is not entirely clear.This article briefly reviews the relationship between BPV and ischemic stroke.

Objective To study the regulatory effect of paclitaxel injection on vascular endothelial related factors in serum of uterine cancer patients.Methods 30 cases of uterine cancer patients treated with paclitaxel (observation group)and 30 cases of uterine cancer patients treated with surgery (control group)were selected.Chemiluminescence immunoassay (CLIA)was used to detect expressions of serum tumor necrosis factor(TNF), Interleukin-8(IL-8)and tumor specific growth factor (TSGF).Results Before treatment,there was no significant difference between the serum TNF, IL-8 and TSGF in observation group and control group(P<0.05).At 3 months after treatment,the serum TNF,IL-8 and TSGFwere significantly lower than before treatment ,which was statistically significant(P<0.05).Conclusion Paclitaxel can decrease serum TNF,IL-8 and TSGF in patients with uterine cancer,which provides a new theoretical basis for its anticancer effects.

Objective To study the characteristics and prognostic analysis of 83 cases of pulmonary infection in elderly patients in emergency department.Methods Thirty-eight elderly patients with pulmonary infection from January 2013 to December 2015 in our hospital were retrospectively analyzed.Results The number of neutrophils increased in 56 patients(67.46%), the neutrophil-lymphocyte ratio increased in 69 patients(83.13%), and C-reactive protein increased in 74 patients increased(89.16 %).There was significant difference between C-reactive protein detection and neutrophil count(P<0.05).61 cases(73.49%) were complicated with other diseases.While the effect of a single antibiotic treatment is less effective.Conclusion The early symptoms of pulmonary infection in the elderly are easy to ignore, and often associated with other diseases.C-reactive protein, neutrophil-lymphocyte ratio combined with lung imaging can be more accurate response to the elderly pulmonary infection.Early detection and early treatment, combined use of antibiotics can improve the prognosis.

Objective To investigate and analyze the diagnostic significance of humoral and cellular immune function detection in the patients with syphilis.Methods Eighty-six cases of syphilis without anti-syphilis treatment in our hospital were selected as the research subjects(syphilis),including 26 cases of primary syphilis,39 cases of secondary syphilis 21 cases of latent syphilis,and 30 healthy subjects were selected as the control group.Then the humoral immunity and cellular immunity levels were compared between the two groups.Results The levels of IgG,IgA and IgM in the syphilis group were significantly increased compared with the control group(P<0.05).The IgG,IgA and IgM levels had no statistical difference between the primary syphilis patients and control group(P>0.05),which in the patients with secondary syphilis and latent syphilis were significantly increased compared with the control group,moreover the increase in the secondary syphilis was most significant(P<0.05);the CD3+,CD4+,CD4+/CD8+ and NK levels in different stages of syphilis were significantly decreased compared with the control group(P<0.05),while the CD8+ level was significantly higher than that in the control group(P<0.05),in which the change in the secondary syphilis patients was most significant.Conclusion The humoral immune in the patients with syphilis is hyperfunction,while cellular immune is dysfunction,the patients present the overall immune function disorder,therefore the humoral and cellular immunity detection has a certain clinical value in the diagnosis of syphilis.

Objective: To study optimum inclusion process conditions for volatile oils from Herba Schizonepetae and Pericarpium Citri Reticulatae Methods : The grinding method was compared with the saturated water solution method by determining the ultilization ratio of volatile oil from both herbs. The optimum preparation conditions were investigated by the orthogonal design. The quality of inclusion compound of volatile oil before and after being included were analyzed by TLC. Results : The optimum preparation conditions for inclusion were established as follow ultilization ?-CD and oil was 8∶ 1, The proportion of water and ?-cyclodextrin was 4∶1 (g:mL), and inclusing time up to three hours, The ultilization ratio of oil was 87.1% . Conclusion : The grinding method is better than the saturated water solution method. The method is applicable.

Objective To understand the present situation of therapeutic drug monitoring(TDM)on cyclosporine A(CsA) application in renal transplant recipients.Methods The clinical data of the first postoperative month of 50 renal transplant cases admitted to our hospital during May 2000 to March 2005 were collected and retrospectively analyzed.Results Total 50 cases(male∶female= 3∶1) had accepted the trigeminy of CsA,prednisone and azamun.The average initial dose and average maintenance dose for the male were higher by 59.94 mg/d and 52.00 mg/d than that for the female(P