Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

The initiation and development of diabetic chronic complications is associated not only with the level of blood glucose,but also with the glucose fluctuation.The damage in large artery and microvessel may be caused by glucose fluctuation.There are two causes which induce glucose fluctuation in clinical:one is postprandial hyperglyceamia with not well controlled,the other is hypoglycemia causes by unsuitable therapy.A lot of effective new types of substances were applicated in clinical,such as ?-glucosidase inhibitor,glinide-insulin secretagogus,fast-acting and long-acting insulin analogues.With these new powerful tools,there is possibility to treat diabetes more correctly and safely,and control the blood glucose in a stable and good level.

The incidence of malnutrition among patients with chronic kidney disease (CKD) constantly increases despite the development of health care and dialysis techniques.New nutritional targets for CKD patients upon maintenance treatment have been developed.Before dialysis,evidences have shown that a long-term nutritional care plan,with a control of protein intake,can effectively correct metabolic disorders including proteinuria and acidosis.During the maintenance dialysis,the optimal protein and energy intakes have been recently challenged.The new classification of nutritional disorders in CKD patients may help the easily identification of protein-energy wasting.

Humans ; Osteotomy ; adverse effects ; methods ; Scoliosis ; surgery

Humans ; Minimally Invasive Surgical Procedures ; Spine ; surgery

Arthroplasty, Replacement, Hip ; trends ; China ; Hip Prosthesis ; Humans ; Prosthesis Failure

Medical words distinguish medical paper from any other papers,and the right choice of medical words plays an important role in conveying medical content.However,with respect to the whole text,a larger number of words are still nonmedical ones,to which great importance should also be attached.In the writing of a medical paper,three principles of exactness,conciseness,and readability in selecting nonmedical words should be followed to make the paper more internationally accepted.

Narcolepsy is a disabling sleep disorder characterized mainly by irresistible excessive daytime sleepiness.It seriously influences patient's academic,family,and social function.The etiology of narcolepsy is still elusive,but the hereditary and environmental factors have been suggested to contribute the most.It is a multi-gene regulation disease,the researches of Human Leucocyte Antigen(HLA) gene type HLA-DQB1 * 0602 and HLA-DRB1 * 1501 are most popular in recent years.Especially the HLA-DQB1 * 0602 is regard as the major HLA susceptibility allele for narcolepsy almost in all ethnic groups.And the hypocretin excreting neurons and the hypocretin's deficits would directly lead to somnolence.Some environmental factors which are newly found these years are still playing important roles,such as the contact with certain antigens,infection of beta hemolytic streptococcus,passive smoking and so on.

This paper presents the writing problems in English abstracts of medical pa-pers,analyzes the underlying causes of these problems,and recommends the strategies for writing internationally standard English abstract.

Objective: To examine the effects of alginate on bone mesenchymal stem cells(bMSCs).Methods: bMSCs were cultured on the alginate gels(experimental group) and tissue culture plastics(control group);the morphous of bMSCs was observed by microscope after toluidine blue staining;the growth curves were analyzed by MTT;and the expressions of osteoblast related genes(alkaline phosphatase,collagen I and osteonectin) were detected by RT-PCR when cultured for 6 days.Results: There were no difference in the cell proliferation between the experimental group and control group.The morphous of bMSCs in the experimental group was induced to be similar to that of osteoblasts and form colony proliferation obviously.The alkaline phosphatase and collagen I genes were positive in the two groups,but their expressions in the experimental group were higher;at the same time,osteonectin gene was positive in the experimental group and negative in the control group.Conclusion: bMSCs may differentiate into osteoblasts automatically when cultured for longer time and that alginate gels could promote this differentiation and may be suitable biomaterial of scaffold in bone tissue engineering.