Objective To detect the levels of Th1 and Th2 type cytokines of peripheral blood CD+4 T lymphocytes in the patients with cervical cancer in order to find out the laboratory evidence of tumor immunotherapy. Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance expression of cytokines. Then specific fluorochrome-conjugated monoclonal antibody was added to be combined with its specific antigen. Results The level of IL-2 produced by Th1 cells were lower in patients of cervical cancer than those of normal controls, there were significant difference between them. But the level of IFN-? produced by Th1 cells had no significant change in patients of cervical cancer in comparison with normal controls. The levels of Th2 type cytokines such as interleukin-4(IL-4), interleukin-6(IL-6), interleukin-10(IL-10) were higher in patients of cervical cancer than those of normal controls, there were significant difference between them. Conclusion Th1/Th2 is imbalance in cervical cancer .Th1 type cytokines are inhibited, and Th2 type cytokines are relatively enhanced, so Th1/Th2 shifts to Th2. It may be the mechanism of tumor arising and transferring by immune escape from immunosurveillance.

After being introduced to Japan, the Chinese acupuncture and massage therapy has changed a lot, in which the influence aroused by the blind practitioners cannot be ignored. Through analyzing the development and the transition of the blind acupuncture and massage industry in Japan, it is found that the tube needle technique, changeable acupoints concept, technical deviation and the importance on acupoints rather than meridians are still existed commonly today, which are introduced by the blind acupuncture and massage practitioners, the special group in Japan. In the process of development, the interaction with the governmental strategy has played the essential role in the consolidation of the above features.
Acupuncture ; education ; history ; manpower ; Acupuncture Points ; Acupuncture Therapy ; history ; History, 15th Century ; History, 16th Century ; History, 17th Century ; History, 18th Century ; History, 19th Century ; History, Ancient ; History, Medieval ; Humans ; Japan ; Massage ; history ; manpower ; Visually Impaired Persons

Objective To observe the effect of Diosmin Tablets about the prevention and treatment of edema that breaks out after a circular mixed hemorrhoids operation and the role of fibrosis on edema.Methods Having 120 cases of circular mixed hemorrhoid patients, Acco 60 cases were randomly divided into treatment group and the others were in comparison group.Both groups were outside the cut line of circular mixed hemorrhoid ligation within the injection, after these operations do conventional anti-inflammatory, hemostasia and local Ma Ying Long musk hemorrhoids ointment medication.The next day of the operation, the treatment group take a dose of Diosmin tablets 1.5 g bid 4 d, then changing the dose 1 g bid 3 d.ResultsThe incidence of edema, treatment group was 6.6% and the other group was 30%.edema in the incidence of the two groups were significantly different(P ＜ 0.01).The treatment group non-fibrosis, but the other group have 4 patients getting fibrosis.The two sets of rate about edema fibrosis were significantly different(P ＜ 0.01).Conclusion Diosmin Tablets can effectively do prevent and disperse circular mixed hemorrhoid postoperative edema.

0.05. Conclusion: The suppressive effect of MSC on patients with ITP in vitro showed no difference from that on the controls.

BACKGROUND:Platelet lysate has been known as a kind of lysate of autologous or alogeneic platelet-rich products. It not only removes the residual cel structure, reduces immunogenicity, but also retains many growth factors. Platelet lysate has been suggested as a substitute for fetal bovine serum to expand mesenchymal stem cels in vitro. OBJECTIVE:To observe the effects of platelet lysate on biological characteristics of human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels, and provide some experimental data for clinical cel therapy and regenerative medicine. METHODS:Platelet lysate was prepared by repeated freezing and thawing from fresh blood. Healthy adult bone marrow and adipose tissue were colected. Human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were obtained by density gradient centrifugation and type I colagenase digestion. We tested the morphology, cel phenotype, differentiation characteristics, proliferation capacity, colony forming ability and the level of cytokine secretion of bone marrow mesenchymal stem cels and adipose mesenchymal stem cels after cultured with platelet lysis. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were successfuly cultured in vitro using platelet lysate. There were no significant differences in morphology, cel phenotype, colony forming ability and the level of cytokine secretion, and chondrogenic, osteogenic and adipogenic capacities between bone marrow mesenchymal stem cels and adipose mesenchymal stem cels. Adipose mesenchymal stem cels had a high cumulative population doublings than bone marrow mesenchymal stem cels (P < 0.05). These findings suggest adipose mesenchymal stem cels had a stronger proliferative ability, and are more suitable for large-scale expansionin vitro cultivation system of platelet lysate compared with bone marrow mesenchymal stem cels.

BACKGROUND:Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells. OBJECTIVE:To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media. METHODS:A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of“mesenchymal (stromal) stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium”in English and Chinese, respectively. Final y, 41 articles were retained. RESULTS AND CONCLUSION:Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages;therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.

Background Studies have showed an increasing prevalence of glaucoma in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS).However,this view remains controversial.Objective This Meta analysis was to assess whether there is an association between glaucoma and OSAHS.Methods A systematic search in PubMed database was carried out with the subject headingssleeping apnea andglaucoma.The literature type was limited to cases-controlled studies and prospective cohort studies with the publishing duration from January 1,1982 to January 30,2015 in English.The risk of glaucoma among OSAHS patients was analyzed,and Review Manager 5.2 was applicated for Meta analysis.The pooled odds ratio (OR) and 95％ confidence interval (CI) was calculated to assess the strength of the association.Results Twelve independent retrospective casescontrolled studies were included in this review,including 11 592 subjects in the OSAHS group and 25 931 subjects in the control group.The study quality was scored 6-8 by Newcastle-Ottawa Scale which was acceptable.The random effects model was accepted because of the heterogeneity among the studies (x2=34.20,P＜0.05,I2 =68％).The prevalence of glaucoma was higher in the OSAHS group than that in the control group (OR =1.87,95％ CI:1.21-2.90,Z=2.82,P＜0.05).The subgroup analysis of OSAHS showed that the OR (95％ CI) of mild,moderate and severe OSAHS groups versus control group was 3.61 (0.56-23.43),4.17 (0.47-36.91) and 6.95 (1.14-42.26),respectively.Sensitivity analysis confirmed that the OR value fluctuated in 1.74-2.16 and closed to 1.87.Funnel graphy exhibited a asymmetry appearance among the literatures,which suggested a possible publication bias.Conclusions OSAHS is one of the risk factors of glaucoma.The serious OSAHS is associated with an increased risk of glaucoma.

In this paper,we study on turbidity-toxin from the connotation,the pathological characteristics and process of diabetes,and explored the relationship of type 2diabetes with abnormal lipid metabolism and lipid toxicity.We found that insulin resistance,apoptosis of Islet,cell and various complications caused by abnormal lipid metabolism and lipid toxicity had signifi cant correlation with the process of the generation and variation of turbidity-toxin:it was argued that blood turbidity and its subsequent toxin accumulation were the pathogenic basis of the formation,combing with the turbidity-toxin,phlegm and blood stasis,then various complications occurred.So it provided a good theoretical and practical foundation for further treatment of type 2 diabetes associated with abnormal lipid metabolism by removing turbidity and detoxication.

Acomplexcascadeofmoleculareventsisconsiderdtobeinitiatedfollowingcerebralis chemia ,whichincludereleaseofexcitatoryaminoacid ,calciumdyshomeostasis ,freeradicalinjury ,increased cytokines,caspaseactivationandalteredgeneexpression .Thisreviewprovidesanoverviewofmolecular mechanismsinvolvedinpostischemicneuronaldeathandanalysesthepotentialfortherapeuticintervention .

Objective To describe self-care agency and its influence factors of the patients with temporary ileum stoma. Methods The descriptive statistics of non-experimental study was performed in the study. The convenience sample consisted of 144 patients from 3 hospitals in Shanghai. The self-care agency was measured by the Colostomy Self-care Agency Scale. Results 144 cases of colorectal cancer temporary ileum stoma patients′ self-care ability scored an average of 57.02 ± 7.00, including self- care willingness (37.19 ± 4.04) points, self-care knowledge 17 points, self-care skills 3 points. The multiple stepwise regression analysis showed that seven factors were statistically significant including age, gender, culture level, living area, postoperative months, presence of complications, whether to accept professional nursing training or education. Conclusions Temporary ileum stoma in patients of rectal cancer with medium self-care ability, but self-care skill level is relatively not high and should be strengthen attention in patients with rectal cancer undergoing ileum stoma. Targeted nursing intervention should be carried out to improve the self-care ability and improve the quality of life of patients with stoma.