Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

The incidence of malnutrition among patients with chronic kidney disease (CKD) constantly increases despite the development of health care and dialysis techniques.New nutritional targets for CKD patients upon maintenance treatment have been developed.Before dialysis,evidences have shown that a long-term nutritional care plan,with a control of protein intake,can effectively correct metabolic disorders including proteinuria and acidosis.During the maintenance dialysis,the optimal protein and energy intakes have been recently challenged.The new classification of nutritional disorders in CKD patients may help the easily identification of protein-energy wasting.

The initiation and development of diabetic chronic complications is associated not only with the level of blood glucose,but also with the glucose fluctuation.The damage in large artery and microvessel may be caused by glucose fluctuation.There are two causes which induce glucose fluctuation in clinical:one is postprandial hyperglyceamia with not well controlled,the other is hypoglycemia causes by unsuitable therapy.A lot of effective new types of substances were applicated in clinical,such as ?-glucosidase inhibitor,glinide-insulin secretagogus,fast-acting and long-acting insulin analogues.With these new powerful tools,there is possibility to treat diabetes more correctly and safely,and control the blood glucose in a stable and good level.

Arthroplasty, Replacement, Hip ; trends ; China ; Hip Prosthesis ; Humans ; Prosthesis Failure

Humans ; Minimally Invasive Surgical Procedures ; Spine ; surgery

Humans ; Osteotomy ; adverse effects ; methods ; Scoliosis ; surgery

Objective To discuss the application and effect of evaluation system in the experiment teaching of pathogenic biology and immunology.Methods The study was based on result of the peacetime assessment,operating test and theory test of 1303 clinical medicine students of Grade 2013.Reversion 3.2.3 was used to calculate the normal distribution,difficulty index,discrimination index and correlation coefficient,and then the characteristics of the assessment methods and the relationship among the components were analyzed.Result The operation test and theoretical test showed a good normal distribution,but the peacetime result showed a certain negative skewness.It was an easy evaluation model because the difficulty index of the examination system was more than 0.70.In peacetime grade,the proportion of high scores accounted up to 62.09％,and the degree of differentiation was low,only about 0.10.In the evaluation system,there were significant positive correlations (r＞0,P＜0.01) in the peacetime,operation and the theoretical results.Conclusion The performance,operation and theoretical results according to the 3:3:4 ratio is reasonable,but some of the problems need to be improved.

Systematic evolution of ligand by exponential enrichment(SELEX) is a new combinational chemical methodology for in vitro selection of specific aptamers.Aptamers are artificial oligonucleotide ligands with high-affinity binding to target molecules,such as amino acids,drugs,proteins and other small molecules or ions and so on.They are isolated from combinational libraries of synthetic oligonucleotide by an iterative process of affinity selection,recovery and amplification.Several properties of aptamers make them an attractive class of therapeutic compounds.Their affinity and specificity for a given protein make it possible to isolate a matching ligand,and to adjust their bioavailability.Aptamers may be useful in the treatment of a variety of human diseases including infectious illness,cancer and cardiovascular diseases.The development of this emerging class of therapeutics is reviewed,in terms of progress,and potentially clinical application.

This paper gives a comprehensive analysis of the origin,formation as well as feature of medical English words in the hope of more accurate understanding and expression in their translation.

This paper puts forward the issue of offering medical English translation course in medical colleges or universities on the basis of social and individual needs analysis.Furthermore,the course is discussed in the respect of materials design and methodology.