Objective To investigate the cytocompatibility of the liching liquids of Co-Cr alloy and Ti alloy on human gingival fibroblasts (HGF). Methods The HGF were treated in vitro with leaching liquids of Co-Cr alloy and Ti alloy, respectively. The DMEM cell medium containing 10% fetal bovine serum was served as a negative control. The viability of HGF treated by two dental alloys were evaluated by means of MTT, and the contents of intracellular reduced glutathione (rGSH) were assayed by kits. The tumor necrosis factor-α(TNF-α) contents were determined in the culture supernatant by ELISA in two groups. The effects of these alloys on the expression of caspase-3 were examined by real time-PCR method. Results Compared with the control group, HGF treated with Co-Cr alloy leaching liquids showed a lower viability ( P ＜0.05), while Ti alloy leaching liquid promoted the proliferation of HGF. In Co-Cr alloy group, the rGSH content was significantly decreased (P＜0.05), while TNF-α content was significantly increased (P＜0.05) compared with control group. There were no significant differences in rGSH and TNF-α contents between the Ti alloy group and control group (P＞0.05). The expression of caspase-3 was significantly higher in Co-Cr alloy group than that of control group (P＜0.05), while there was no significant difference in the expression of caspase-3 between Ti alloy group and control group. Conclusion Results suggest that Co-Cr alloy possesses cytotoxicity, while there is better cell compatibility for Ti alloy.

OBJECTIVETo explore the effect of Chinese drugs for activating blood circulation and removing blood stasis (CDABCRBS) on carotid atherosclerotic plaque and long-term ischemic cerebrovascular events.

METHODSBy using open and control method, effect of 4 groups of platelet antagonists, platelet antagonists + CDABCRBS, platelet antagonists +atorvastatin, platelet antagonists +atorvastatin +CDABCRBS on carotid atherosclerotic plaque and long-term ischemic cerebrovascular events of 90 cerebral infarction patients were analyzed.

RESULTSThrough survival analysis, there was no statistical difference in the effect of the 4 interventions on the variation of carotid stenosis rates or ischemic cerebrovascular events (P > 0.05). The occurrence of ischemic cerebrovascular events could be postponed by about 4 months in those treated with platelet antagonists + CDABCRBS and platelet antagonists + atorvastatin +CDABCRBS. By multivariate Logistic analysis, age, hypertension, and clopidogrel were associated with stenosis of extracranial carotid arteries (P <0.05). Age, diabetes, aspirin, clopidogrel, CDABCRBS were correlated with cerebrovascular accidents (P < 0.05).

CONCLUSIONSWhether or not accompanied with hypertension is an influential factor for carotid stenosis, but it does not affect the occurrence of ischemic cerebrovascular events. CDABCRBS could effectively prolong the occurrence time of ischemic cerebrovascular events.

Adult ; Aged ; Brain Ischemia ; etiology ; Carotid Artery Diseases ; complications ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Stroke ; etiology

The accelerating pace of exchanges and cooperation in medical field between China and foreign countries demands a large number of medical papers, medical-related materials to be ac-curately translated into multiple languages , especially between English and Chinese . Oriented at English majors (medicine directed), based on eco-translatory approach and student-centered teaching method by creating simulations of the medical English ecological translation environment, this paper provided a better and effective practice-based teaching idea, which can promote students' medical English translation competence and strategies, even cultural consciousness and pragmatic ability in a comprehensive manner.

10.3969/j.issn.1000-8179.2013.12.007

Objective To investigate the correlation between epicardial fat thickness (EAT) and the risk factors of coronary artery disease (CAD) as well as the severity of coronary lesions with echocardiography,Methods According to the results of coronary angiography,80 patients underwent coronary angiography were divided into coronary heart disease group (CHD group,4 6 cases) and without coronary heart disease group (control group,34cases),and according to coronary artorrythe severity,CHD goup was divided into subgroups of single-vessel disease (24 cases) and multivessel disease subgroup(22 cases).Tho epioardial fat thickness on the free wall of the right ventricle was measured at end-systole from the para-sterna,long-axis vlews.The relationship between EAT and the risk factors of CAD (such as age,gender,hypercholesterolemia,hypertenston,diabetes,smoking,family CAD history) as well as the severity of coronary lesions were analyzed.Results EAT in multivessel disease CAD group [(8.85 ± 1.44)mm] was significantly higher than that in single vessel CAD group[(6.86 ± 1.09) mm] and control group [(4.58 ± 1.12) mm] (all P ＜ 0.01).EAT was significantly related with the risk factors of CAD.Although the risk factors of CAD such as age,blood glucose,blood pressure were adjusted,EAT was still independently associated with CAD (OR:5.394,95 ％ CI:1.700 ～ 17.1 12,P =0.004).Concluslon The level of EAT is significantly correlated with CAD and the severity of coronary lesions.

To observe the pathological changes of vascular aging in spontaneously hypertensive rats (SHRs), and the effects of extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong on vascular aging.

Objective B-type natriuretic peptide(BNP) have been used widely in the diagnosis and prognosis of cardiogenic diseases, but the association between BNP concentrations and non-cardiogenic critical illness is unknown. Our study aims at investigating the role of natriuretic peptide testing for predicting 28-day mortality of non-cardiogenic critically ill patients in emergency department (ED). Methods A total of 255 non-cardiogenic critically ill patients treated in the emergency department were prospectively enrolled and a sample of blood was obtained for BNP measurement from each patient. A variety of clinical and laboratory variables were recorded. After 28 days, the vital status of each patient was ascertained and the association between BNP values at presentation and mortality was assessed. Results 71 patients died during the 28-day observation period. Median BNP concentrations at presentation among decedents were significantly higher than those of survivors (326. 0 ng/L versus 50. 9 ng/L,P <0. 001). The optimal BNP cut point for predicting 28-day mortality was 114. 0 ng/L. In a multivariate analysis (Cox-regression), a BNP concentration greater than 114. 0 ng/L(RR 7. 268, 95% CI 3. 864-13. 672) and an Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) scores greater than 20 (RR 3. 330, 95% CI 1. 815-6. 109)were the independent predictors of the 28-day mortality. BNP concentration alone had an area under the receiver operating characteristic curve (AUC) of 0. 825 for predicting mortality. Conclusions BNP concentrations at presentation are strong predictor of 28-day mortality in patients with non-cardiogenic critically ill patients in ED, which is better than Rapid Acute Physiology Score (RAPS) , Rapid Emergency Medicine Score (REMS) and APACHE Ⅱ score.

The brief discussion is introduced in the paper on the academic thought of professor YAN Jie, the contemporary famous TCM doctor, on functional dyspepsia treated with acupuncture and moxibustion. Treatment based on "the three-regional acupoint selection" is applied to professor YAN's treatment for functional dyspepsia, in which, acupuncture is on Sibai (ST 2), Liangmen (ST 21) and Zusanli (ST 36), and the supplementary points are added accordingly. The academic thought is described as the combination of acupuncture and moxibustion based on strengthening healthy qi, supplemented by soothing the liver and psychological counseling. Also, an example is provided.
Acupuncture ; education ; history ; Acupuncture Points ; Acupuncture Therapy ; history ; Adult ; Dyspepsia ; history ; therapy ; Female ; History, 20th Century ; Humans ; Moxibustion

Objective To evaluate influence of benzo(a)pyrene on cell cycle of the human embryonic lung fibroblast at different synchronous stage. Methods Cell cycle distribution were detected by flow cytometry(FCM). Cells were synchronized at cycle G0 stage through 48 hours serum starvation and reenter cell cycle together at a synchronous state after resupplied with whole growth medium contain good serum. Benzo(a)pyrene with a series concentrations were directly used or metabolized active by traditional and modified metabolize active methods through preincubation of S9 and were treated as regent to HELF cells mostly synchronized at G1, S or G2-M stage respectively. Then cell cycle distribution changes were detected 12 h after treatment. Results Serum starvation(48 hours) could synchronize HELF at G0-G1 stage effectively and 10-12 h, 16-18 h, 22-24 h were cycle phase change distinctly time after serum restimulated. Cells were synchronized at G1, S and G2-M stage mostly. Benzo(a)pyrene influence cycle distribution little without metabolize active, while modified methods could metabolize active benzo(a)pyrene well and avoided disturbing effect of S9 on cell cycle in traditional method. Except 2 ?mol/L benzo(a)pyrene treated at 22 h after restimulated caused the percentage of cells at S stage increase, most treatment induced the percentage of cells at S stage decrease which was associated obviously with the increasing dosages. The percentage of cell at S stage decrease at 16 h were more remarkable than other times and the percentage of cell at G2-M increase correspondingly. While the percentage of cell at G1 increase and the percentage of cell at G2-M decrease were obsered when benzo(a)pyrene treated at 10 h and 22 h after restimulated. Conclusion Serum starvation 48 hours and restimulate can synchronize HELF at different stage effectively. Modified metabolize active method is suitable for cell cycle research. Primary influence of benzo(a)pyrene on cell cycle is the decreased percentage of cells at S stage, G1 arrest, G2-M arrest and G1 arrest were occurred when benzo(a)pyrene were treated at G1, S and G2 stage respectively.

In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.