AIM: To optimize technique for preparing curcumin solid lipid nanoparticles by film-ultrasonic dispersion. METHODS: The influence of various factors,including the consumption of curcumin,stearic acid,lecithin and tween-80,and their effects on the entrapment efficiency and the ratio of loading drug were investigated.And the optimum formula was selected through uniform design test. RESULTS: Under the condition of 30 mg of curcumin,60 mg of lecithin,117 mg of stearic acid and 10 mL of tween-80(1.0%) curcumin solid lipid nanoparticals had good size distributions.The mean particle size diameter was 58.0 nm and the average entrapment efficiency and the ratio of loading drug approached 95.98% and 15.87%,respectively. CONCLUSION: The technique for preparing curcumin solid lipid nanoparticals N by film ultrasonic method is feasible.

OBJECTIVE To analyze and evaluate the risk factors,clinical characteristics and prevention counter-measures of hospital fungal infection in patients with gynecological malignant tumors.METHODS The clinical data of hospital fungal infection in 19 cases from 213 tumor patients were reviewed.RESULTS The infection rate was up to 8.92%.The locations of infection were genital tract(73.68%) and then the mouth cavity(21.05%);the key fungi were Candida albicans and C.glablata.CONCLUSIONS The key to reduction of hospital fungal infection after malignant tumors is to reduce susceptible factors,enhance immunity and timely examine and treat the infection.

Objective To study the diagnostic value of myocardial damage by combined determination of cardiac troponin I(cTnI),myoglobin(MYO) and creatine kinase isoenzyme MB mass(CK-MB mass) in neonates with hypoxic ischemic encephalopathy(HIE).Methods The concentrations of serum cTnI,MYO and CK-MB mass were measured in 148 patients with HIE(61 mild,52 moderate and 35 severe) and in 60 healthy subjects by microparticle chemiluminescent immunoassay.Results The study revealed that patients with severe and moderate HIE had sighifieant high levels of cTnI,MYO and CK-MB mass as compared with those with mild HIE and controls(analysis of variance ,P0.05).Not only in mild HIE but also in moderate HIE as well as in severe HIE in the first day of life,the abnormal ratio of MYO were the highest.In the third day of life,the seroprevalence of cTnI,MYO and CK-MB mass in mild HIE group were 6.56%,9.84% and 8.20%,respectively;and those of moderate HIE group were 42.31%,44.23% and 42.31%,respectively;and severe HIE group had the highest abnormal ratio of cTnI(77.14%),MYO(74.29%) and CK-MB mass(77.14%).After the third day,the concentration of MYO in three HIE groups kept reduced but the CK-MB mass and cTnI increased to the peak and maintained above one week at higher levels.Conclusion There is a close association between the cardiac dysfunction and cerebral injury in neonates with HIE.The more cerebral injury the patients have,the more severe cardiac dysfunction they would suffer.

Diabetic retinopathy (DR),one of the most serious microvascular complications of diabetes mellitus,leads to the increasing blindness rate,which seriously affects the quality of life in diabetic patients.Although the actual mechanism of DR is not yet completely clear,mounting evidence suggests that the development of DR may be associated with inflammatory processes and inflammatory factors,such as C-reactive protein (CRP),chemotatic factor,adhesion molecule,interleukin,tumor necrosis factor-α (TNF-α),serum amyloid A (SAA).Usage of glucocorticoids,non-steroidal anti-inflammatory drugs(NSAIDs),minocycline and anti-inflammatory factors to inhibit inflammatory process would be a new approach to the treatment of DR.This article reviews the current studies about the effects of inflammatory reaction and inflammatory factor in the pathogenesis of DR,mechanism of suppressing inflammatory response and anti-inflammatory factor in the treatment of DR.

It is necessary to have a research on dental fluorosis in a population to minimize the prevalence and severity of caries.It is important to research the pathogenesis of enamel fluorosis and prevention and control of dental fluorosis and caries.This article reviews the progress in chemistry and biology of dental fluorosis.

Objective: To observe the histomorphology/pathology changes and the expression of hypoxia inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in drowning rats brain, in an attempt to explore new pathways for treatment of water drowning. Methods: The animal model of freshwater drowning was established with rats. The rats were randomly divided into the following groups: control group, drowning-to- death group and 2 min-drowning group (including 5 subgroups survived for 3, 6, 12, 24, and 40 hours after being drowned in water for 2 min [n=10]). Brain indices of rats in each group were detected and the pathological changes of cerebral tissues were observed; the expression of HIF-1α and VEGF were detected immunohistochemically and the correlation between them was analyzed. The dynamic blood oxygen saturation was detected in rats survived for 40 h after 2 min-drowning at different points. Results: Brain index of the drowning group and groups surviving for 24, 40 hours after drowning were obviously higher than that of the control group (P<0.05). Brain indices of the drowning-to-death group and the 24 h, 40 h survival groups were obviously higher than that of the control group; and those of the 3 h, 12 h survival groups were obviously lower than that of the drowning-to-death group but higher than that of the normal control group (P<0.05). The blood oxygen saturation was (59=5)% immediately after drowning and then gradually increased and reached the normal level after 12 h. The cerebral edema appeared 3 h after drowning and was gradually aggravated from 12 h to 24 h after drowning, then slightly relieved 40 h after drowning. There was no expression of HIF-1α in the control group and lower expression in drowning-to-death group; the expression of HIF-1α gradually increased with the increase of drowning period and reached peak 12 h after drowning(P<0.05); the expression of VEGF had a similar pattern to HIF-1α. The expression of HIF-1α was positively correlated with that of VEGF(r=0.629,P<0.05). Conclusion: Freshwater drowning can cause serious cerebral injury. The brain can be protected from oxygen deficiency by promoting synthesis of HIF 1α and subsequent upregulation of VEGF expression.

Objective To compare the changes of corneal endothelial cells in early recovery period after 1.8 mm small incision phacoemulsification nucleus chopping and conventional incision phacoemulsification for cataract.Methods Fifty-three patients with cataract were divided into the observation group and control group according to different operation methods.The observation group was treated with 1.8 mm small incision phacoemuisification nucleus chopping while the control group was treated with routine incision phacoemuisification.The visual acuity,degree of astigmatism,corneal endothelial cell indexes and complications were observed.Results The effective ultrasound time and average ultrasonic energy of the observation group were significantly less than those of the control group (all P ＜ 0.05).Compared with 1 day before operation,the visual acuity,degree of astigmatism,corneal endothelial cell area (ECA) and coefficient of variation (CV) were significantly increased at 1 week and 4 weeks after operation,and corneal endothelial cell density (ECD) was significantly decreased (all P ＜ 0.05).The visual acuity and ECD levels of the observation group at 1 day,1 week and 4 weeks after operation were significantly higher than those of the control group,while the degree of astigmatism,ECA and CV were significantly lower than those of the control group (all P ＜ 0.05).Conclusion Compared with the traditional incision phacoemulsification,the damage of 1.8 mm small incision phacoemulsification nucleus chopping to corneal endothelial cells is significantly milder,can reduce the effective ultrasound time and average ultrasonic energy,decrease the degree of astigmatism,improve the visual acuity and decrease the corneal edema.

Objective To analyze the effect of partial nephrectomy with three-dimensional laparoscopy for the treatment of renal tumor.Methods From October 2013 to September 2015, 11 patients underwent 3D laparoscopic partial nephrectomy for renal tumor in our hospital.All the cases were given retroperitoneal approach.The renal artery was seperated and blocked, and the renal tumor was exposed and completely resected around the edge.The wound was double layer sutured with Vicryl.Results All the 11 operations were finished smoothly without conversion to open surgery.The operative time was 70-180 min (mean, 100 min), the ischemic time was 14-35 min (mean, 25 min), and the estimated blood loss was 50-300 ml (mean, 90 ml).There were 1 case of intraoperative peritoneal perforation, 2 cases of subcutaneous emphysema, and 1 case of perirenal hematoma.No other operative or postoperative complications occurred.The post-operative hospital stay was 5-10 d (mean, 6 d).Post-operative follow-ups for 3-14 months (mean, 10 months) in 10 cases found no recurrence or metastasis or renal dysfunction.Conclusions Partial nephrectomy of renal tumor with three-dimensional laparoscopy is safe and feasible.The outcome is satisfying and worthy of application.

Objective The research of craniocerebral injury and fracture healing has made slow progress, and the lack of reliable animal experimental model is one of the key factors.Therefore, the focus is to establish a craniocerebral injury and fracture model up to standard.The study aims to establish an appropriate animal model and evaluate the stability.Methods New Zealand white rabbits were randomly divided into four groups(n=12): control group, simple craniocerebral injury group(simple craniocerebral injury model), simple fracture group(simple right tibial fracture model), Brain trauma with fracture group (craniocerebral injury combined with fracture model).The neurological changes of the rabbits were observed.Furthermore, the healing of the fractures was observed at different time postoperatively by measuring the volume of callus and observing pathology.Results The neurological function scale (NSS) scores [(9.32±0.68), (8.01±0.56), (7.75±0)]6 h, 7 d and 1 month after craniocerebral injury surgery were significantly lower than those in the control group[(11.81±0.55),(12.07±0.42),(12.02±0.38)], and there was statistically significant difference (P<0.05).Compared with the callus volume of simple fracture group around 1 month after surgery, the callus volume of the craniocerebral injury combined with fracture group was significantly larger [(1.19±0.12) cm3 vs (1.81±0.13) cm3, P<0.05].Four weeks after surgery in the craniocerebral injury combined with fracture group, the trabecular bone was significantly increased in the local callus.After 8 weeks, the trabecular bone became more mature.Conclusion The animal model of craniocerebral injury combined with fracture healing is stable and reproducible.It meets the standard for studying the relationship of traumatic brain injury and fracture.

Objective:To investigate the effect of NF-κB p65siRNA of IL-17 inducing the expression of MCP-1 in the primary cultured cardiac myocytes.Methods:The cardiac myocytes were isolated from neonatal mice by different adhesion method.NF-κB P65 siRNA was transfected into cardiac myocytes and the rates of transcription and translation of MCP-1 were detected by RT-PCR and enzyme-linked immunosorbent assay( ELISA) ,the rates of transcription and translation of P-P65 and P65 were detected by RT-PCR and Western blot.Results:Compared with negative siRNA group,the expression of the MCP-1 at mRNA and protein levels were increased in negative siRNA +IL-17 group(P<0.05).Compared with negative siRNA group,the expression of the MCP-1 at mRNA and protein levels were decreased in NF-κB P65 siRNA group( P<0.05).Compared with negative siRNA+IL-17 group,the expression of the MCP-1 at mRNA and protein levels were decreased in NF-κB P65 siRNA+IL-17 group(P<0.05).The expression of the NF-κB P65 at mRNA and protein levels in cardiac myocytes were specifically and extensively suppressed by NF-κB P65 siRNA(P<0.05).After stimulated by IL-17,the amount of P-P65 in the cardiac myocytes was significantly increased in time-dependent manner compared with that of black group( P<0.05) , but the levels of P65 changed little ( P>0.05 ) .Conclusion: IL-17 stimulates MCP-1 expression in cardiac myocytes via NF-κB activation,and NF-κB P65 siRNA can effectively inhibit the upregulation of IL-17 on MCP-1.