1.Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 mRNA in endometriosis
Chinese Journal of Obstetrics and Gynecology 2001;0(01):-
Objective To investigate mRNA expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) in ectopic endometriosis tissue and uterine endometrium from women with and without endometriosis. Methods Thirty-eight women with endometriosis (Revised American Fertility Society classification, RAFSⅠ-Ⅳ) were selected as study group. Thirty-eight specimens of ovarian endometrioma(ovarian chocolate cysts, OCC), 16 red peritoneal endometriotic lesions(RPL), and 35 matched eutopic endometrium(Eu) were collected from them simultaneously at the time of surgery. Twenty specimens of endometrium from reproductive women undergoing laparoscopic surgery without endometriosis were obtained as control group. The mRNA expressions of MMP-9 and TIMP-1 were detected by reverse transcription polymerase chain reaction (RT-PCR). Results Expression of TIMP-1 mRNA was detected in all samples. The level from endometriosis patients and control group was similar(2.31?1.21, 2.40?0.89). However, ectopic endometrium expressed significantly fewer TIMP-1 mRNA(OCC 1.67?0.79, RPL 1.45?0.68)compared with eutopic endometrium from both endometriosis and endometriosis-free patients(P
2."Features and modification of ""emotional inter-resistance"" therapy"
International Journal of Traditional Chinese Medicine 2012;34(5):429-431
To summarize the features and advantages of traditional emotional inter-resistance therapy,propose some existing problems influencing its clinical application and find out corresponding possible modification to improve the feasibility of this therapy in clinical practice.
3.Advaneed Condition and Development Tendency of Contemporary Medical Equipment Management
Yinbao CHONG ; Lang LANG ; Yan HUANG
Chinese Medical Equipment Journal 1989;0(03):-
According to the actuality of domestic medical equipment management and its main problems, development tendency of current equipment management are analyzed in four aspects of the norms of medical equipment management standards and details, the expansion of medical equipment information management in whole course, attention to medical engineering disciplines, and strengthening the quality control of medical equipment and application of security technology.
4.Study of ambulatory EEG in patients with sleep epilepsy
Xiangqing WANG ; Senyang LANG ; Yan LIU
Journal of Clinical Neurology 1988;0(02):-
Objective To explore the feature of epileptiform discharges during day and night in patients with sleep epilepsy and its diagnosis value. Methods Ambulatory EEG (AEEG) was performed continuously for 24 hours in 91 patients with sleep epilepsy. The frequency of epileptiform discharges in wake phase and sleep phase were analyzed. Results Epileptiform discharges were observed in 71 patients. 4 patients showed epileptiform discharges only in wakefulness, 41 only in sleep and 26 both in wakefulness and sleep. 34 patients showed epileptiform discharges only in stage 1 and 2 of NREM sleep, and 33 patients displayed epileptiform discharges in whole cycle of sleep. Seizure frequency in the patients with epileptiform discharges in wakefulness was higher than those without epileptiform discharges or with epileptiform discharges in sleep (both P
5.A high throughput screening model for P-gp functional inhibitors
Tingyuan LANG ; Jufang YAN ; Changhua HU
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To establish a cell model for high throughput screening and search for novel inhibitors of P-gp.Methods A fluorescent cell-based assay was developed for application in high throughput screening.A library of 480 traditional Chinese medicine samples was screened. Results The value of Z′ was 0.72. Screening of a library of 480 traditional Chinese medicine identified 2 positive samples.Conclusions This HTS model for screening P-gp inhibitors was stable and sensitive.The 2 positive samples had good P-gp inhibitory activities.
6.Expression of MT and p53 in Non-small Cell Lung Carcinoma
Xinhua LV ; Lang XU ; Jianhui YAN
Journal of Chinese Physician 2001;0(07):-
Objective To Investigate the expression of metallothionein (MT) and p53 in non-small cell lung carcinoma(NSCLC). Methods The expression of MT and p53 was detected by immunohistochemical method in the paraffin-embedded specimens of 73 NSCLCs and 15 normal bronchial mucosal tissues(NBMT). Results [WTBZ]The positive rates of MT and p53 expression in NSCLC were 54.8%(40/73)and 65.8%(48/73), respectively, and in NBMT were 20.0%(3/15)and 33.3%(5/15), respectively. The positive rates of both MT and p53 in NSCLC were significantly higher than those in NBMT(P0.05). The positive rates of MT and p53 were closely correlated with the histological grades and lymph node metastasis of NSCLC(P
7.Anti-inflammatory Effect of Three Kinds of Aconine from Radix Aconiti Lateralis Preparata on Macrophages
Ruili ZHU ; Lang YI ; Yan DONG ; Peixun WANG
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(5):908-913
Objective To observe the anti-inflammatory effects of three kinds of aconine from Radix Aconiti Lateralis Preparata ( Fuzi) on macrophages, as well as the effects of their combination with sinomenine. Methods The effect of three monoester alkaloids from Fuzi, benzoylaconine ( BAC) , benzoylmesaconine ( BMA) and benzoylhypaconine ( BHA) , on RAW264.7 cells proliferation was detected by cell counting kit 8 (CCK8) in vitro. RAW 264.7 macrophage cells were stimulated by lipopolysaccharides (LPS) and then treated with different concentrations of BAC, BMA, and BHA. Tumor necrosis factor alpha ( TNF-α) and interleukin 6 (IL-6) secreted by RAW264.7 cells were measured by enzyme-linked immunosorbent assay (ELISA) . The anti-inflammatory effects of three monoester alkaloids combined with sinomenine were evaluated by Zhengjun Jin Q method. Results The concentrations of TNF-α and IL-6 secreted by LPS-stimulated RAW264.7 cells were increased significantly ( P<0.01) , and then were inhibited by BAC ( 20, 30 μmol/L) , BMA ( 40, 80, 160μmol/L) and BHA ( 40, 80, 160 μmol/L) significantly ( P<0.01) . Antagonistic effects were present when 30 μmol/L of BAC or 160 μmol/L of BMA or 160 μmol/L of BHA was respectively used together with 100 or 300 μmol/L of sinomenine (Q<0.85). Conclusion The three kinds of monoester alkaloids from Fuzi exert anti-inflammatory effect on macrophages, and the effective dose of BAC is lower than that of BMA and BHA. The combination of BAC, BMA or BHA at the analyzed dosage with sinomenine has antagonistic effect.
8.Effect of sinomenine on mouse RAW264.7 macrophage cells line polarization induced by LPS or IL-4
Jinfang LUO ; Ruili ZHU ; Lang YI ; Yan DONG ; Peixun WANG
Chinese Journal of Immunology 2015;(1):56-60
Objective:To investigate sinomenine (Sinomenine,SIN) effect on RAW264.7 cells polarization to M1 or M2 phenotype induced by lipopolysaccharide (LPS) or interleukin-4 (IL-4) .Methods:RAW264.7 cells were induced to polarize to M1 by LPS ,and to M2 by IL-4.Sinomenine effects on LPS or IL-4 induced macrophages:TNF-αand IL-10 secretion induced by different condition were detected by Enzyme linked immunosorbent assay (ELISA);The expression level of mRNA of Arginase1(Arg-1),Nitric oxide synthase(iNOS),suppressor of cytokine signaling protein-2(SOCS2) and suppressor of cytokine signaling protein-3(SOCS3) of M1/M2 phenotypes were detected by real time PCR respectively.Results:Sinomenine inhibited the increase of TNF-αsecretion,iNOS and SOCS3 mRNA expression level induced by LPS.Sinomenine inhibited the increase of IL-10 secretion and Arg-1 mRNA expression level induced by IL-4,but SOCS2 mRNA expression level was not affected by Sinomenine.Conclusion: Sinomenine can inhibite the macrophage polarization to M1 and M2 induced by LPS and IL-4.Sinomenine plays a regulatory role on imbalance of M1/M2,and is conducive to maintain the dynamic balance.
9.The relationship between the expression of CD44v6,P16 protein and invasion and metastasis in gastric carcinoma
Xinhua LV ; Lang XU ; Wensheng OU ; Jianhui YAN ; Kun LIAO
Journal of Chinese Physician 2001;0(05):-
Objective To explore the relationship between the expression of CD44v6,P16 protein and the invasion and metastasis in gastric carcinoma and to investigate the correlation between the expression of CD44v6 and P16 protein.Methods Immunohistochemical methods were used to detect the expression of CD44v6 and P16 protein in 110 cases of gastric carcinoma.Results In 110 cases of gastric carcinoma,the expression of CD44v6 and P16 protein were 72.7%(80/110)and 47.3%(52/110)respectively. The high level expression of CD44v6 protein and low level expression of P16 protein were positively correlated with the TNM staging,serosa infiltration,lympho node and liver metastasis of gastric carcinoma(P
10.Effects of fluvastatin on the airway remodeling in a guinea pig model of asthma
Liqiang SONG ; Haowen QI ; Yan LI ; Bin LANG
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To investigate the effects of fluvastatin on the airway remodeling in a guinea pig model of asthma. METHODS: Asthmatic guinea pig model was established by intraabdominal injection of ovalbumin and aluminium hydroxide and challenged with ovalbumin once every other day for 60 days. 30 guinea pigs were randomly divided into three groups: control group ( n= 10), asthma group ( n= 10) and fluvastatin plus asthma group ( n= 10) in which fluvastatin was inhaled at concentration of 0.5 g/L 30 min before every challenging. The thickness of airway smooth muscle layers of every three groups were compared after Haematin-Eosin staining by image analysis system. The level of ras mRNA in airway were examined by Dot-blot molecular hybridization. The expression levels of ras p21 were also examined by immunohistochemical technique. RESULTS: The mean thickness of airway smooth muscle in asthma group was (74 27?3 30) micrometer, greater than that of control group [(38 57?3 37) micrometer ( P