OBJECTIVE: To study the effect of cinnamaldehyde ofloxaein-3-ylhydrazone (FQ)16) on apoptosis of human pancreatic carcinoma cells in vitro. METHODS: Human pancreatic carcinoma cell line BxPC-3 was treated with FQ16 at different concentrations. The proliferation inhibition was examined by MTT assay. Morphological examination, TUNEF and agarose gel electrophoresis method were used to detect apoptosis. Western blotting method was used to detect the expression of apoptosis related genes caspase-3 , caspase-9, caspase-8, bcl-2, bax and cytochrome C. RESULTS: The cell proliferation was inhibited by FQ16 in a time- and a dose-dependent manner. Treatment of BxPC-3 cells with different concentrations of FQ16 for 24 h increased the percentage of the apoptotic cells obviously (P < 0.05); the morphology of BxPC-3 showed changes such as nuclear chromatin condensation and fragmentation and typical ladder DNA in apoptotic cells. FQ16 increased protein expression of bax, caspase-9 and caspase-3, and induced cytosolic accumulation of active caspase-9 and caspase-8, whereas the protein expression of bcl-2 decreased. Western blotting results reveal that FQ16 released mitochondrial cytochrome C into cytosol in a dose-dependent manner. CONCLUSION: Apoptosis in pancreatic carcinoma BxPC-3 cells is one of the key mechanisms of action of cinnamaldehyde olloxacin-3-ylhydrazone, and mitochondrial-dependent pathways are involved in the induction of apoptosis of BxPC-3 cells. Copyright 2012 by the Chinese Pharmaceutical Association.

Cardiovascular disease, with high morbidity and mortality, has been threatening the health of human beings. Therefore, expecting to find a more effective therapeutic method, a plenty of researchers devote themselves to the study of the cardiovascular disease all the time. Since discovered on the heart, M3 receptor of muscarinic acetylcholine receptor (mAchR, M receptor) became a new starting point of the research of the cardiovascular disease. With more and more investigation, many people found that M3 receptor could protect the heart from kinds of cardiovascular disease, which may make it a new hopeful therapeutic point. So, expecting to give support to the reference and encouragement for the study of disease related to M3 receptor in future, this review expounds M3 receptor on the heart from the main following aspects: the effect on the heart, the influence on the cardiovascular disease and the mechanism of M3 receptor involved.

Objective:To establish a method for the determination of Mg and Al in 5-layer co-extrusion bags and their migration from the bags into infusion by ICP-MS. Methods:The samples were digested by microwave digestion, and the contents of Mg and Al were determined by ICP-MS under certain working conditions. Results:Both Mg and Al had a linear range between 2. 5 and 20. 0 μg ·L-1, and r was 0. 999 6 and 0. 999 7, respectively. The average recovery of Mg was 92. 90% (RSD=3. 01%,n=9),and that of Al was 94. 15% (RSD=4. 11%,n=9). Conclusion:The method is accurate, sensitive and simple, and can be used for the determi-nation of Mg and Al in 5-layer co-extrusion bags and their migration from the bags into infusion.

Objective:To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide (ASODN).Method:The designed Stat3 ASODN was transferred into laryngeal cacinoma Hep-2 cell by lipofection. Expression of Bcl-2, Bax and C-Myc were detected by Western blot and PCR.Result:Western blot and PCR results demonstrated that Stat3 ASODN could significantly increased the expression of Bax and decreased the expression of Bcl-2 and C-Myc when the concentration of antisense oligodeoxynucleotide were heightened.Conclusion:Stat3 ASODN participate in apoptosis by enhancing the expression of Bax and reducing the expression of Bcl-2 and C-Myc.

BACKGROUND: Superior trunk of brachial plexus nerve is the part where brachial plexus nerve injury occurred easily during the process of delivery.OBJECTIVE: To perform elongation test, stress relaxation and creeping test on superior trunk of brachial plexus nerve of fresh fetal corpses, and to analyze the mechanical characters of elongation and mechanical characters of visco-elasticity.DESIGN: A randomized and controlled experiment, multiple variable analysis.SETTING: Department of Radiology, China-Japan Union Hospital, Jilin University, Department of Engineering Mechanics, Nanling Campus, Jilin niversity.MATERIALS: The specimens were 30 dead and fresh fetuses aged 6.5 to 8 months, including 16 male and 14 female.METHODS: ①After efrosting at ordinary temperature, superior trunk of brachial plexus nerve was taken out, and 100 samples were cut out along the length wise of specimen. 32 samples were used in elongation test, 32 in stress relaxation test and 32 in creeping test and 4 in pre-test. ②Elongation test, stress relaxation test and creeping test were separate and each test was subdivided into 4 groups: male fetus group of 6.5 to 7 months ,male fetus group of over 8 months, female fetus group of 6.5 to 7 months and female etus group of over 8 months, with 8 samples in each group. ③Superior trunk of brachial plexus nerve was performed one-way elongation test ,and destroy load , intensity limit, maximum strain were obtained; Superior trunk of brachial plexus nerve as performed stress relaxation test and creeping test, and data and curve of stress relaxation test and creeping test were obtained. Experimental data were given normalized treatment and normalized stress relaxation function and normalized creeping function and curve were derived. The experimental data were performed regression analysis with one-dimensional linear regression analysis, then regression coefficient was obtained and experimental curve was fitted.MAIN OUTCOME MEASURES: Mechanical characters of elongation and mechanical characters of visco-elasticity of superior trunk of brachial plexus nerve.RESULTS: ①Destroy load, intensity limit and maximum strain of superior trunk of brachial plexus nerve were all less in fetus group of 6.5 to 7 months than in the fetus group of over 8 months (P ＜ 0.05). There was no significant difference of mechanical characters of elongation among brachial plexus nerves between male and female (P ＞ 0.05). ② 7 200 s creeping quantity was larger in female fetus group of over 8 months than in the male fetus group of over 8 months [(10.2±1.1)%, (7.2±1.08)% ,P ＜ 0.05].7 200 s creeping quantity was basically close in female fetus group of 6.5 to 7 months group to male fetus group of 6.5 to 7 months [(16.7± 1.6)%,(15.1±1.2)%,P ＞ 0.05]. ③ 7 200 s stress relaxation quantity was basically similar in male fetus group of over 8 months to female fetus group of over 8 months (P ＞ 0.05). 7 200 s stress relaxation quantity was larger in male fetus group of 6.5 to 7 months than in female fetus group of 6.5 to 7 months (P ＜ 0.05).CONCLUSION: In the process of delivery, at the same external force, only fewer fetuses with poor ability to resist external force present brachial plexus nerve injury. The individual difference of mechanical characters of elongation of superior trunk of brachial plexus nerve might be caused by heredity, dystrophy and other factors. In addition, at the same strain, the creeping quantity is larger in the female group of small month than in the female group of large month, which might be due to that the development of brachial plexus nerve is not mature in the fetus of small months as that in the fetus of large months.

BACKGROUND:Along with the medicine development.the prevention and treatment of the atrophy of denervated skeletal muscle have made a remarkable progress,but the clinical curative effect is still not satisfied OBJECTIVE:To summarize the current status of the prevention and treatment methods of denervated skeletal muscle atrophy,and to find a more effective method for the prevention and treatment of the denervated of skeletal muscle atrophy METHODS:Using"denervation,muscle atrophy,treatment"in English for the search term,Medline database from January 1998 to January 2008 was retrieved.Using"denervation,muscle atrophy,treatment:in Chinese for the search term,the Chinese Journal Full-text Database from January 1998 to January 2008,Wanfang Database from January 1998 to January 2008,and Journal of Clinical Rehabilitation and Tissue Engineering Research from January 1 998 to January 2008 were searched.Literature was limited to English and Chinese languages.The muscular endurance and contractile force,muscle wet weight of denervated nerve,and skeletal muscle repair served as the evaluation index The studies on the micro-surgical procedures,physical therapy,biological and chemical therapies,gene therapy for the atrophy of denervated skeletal muscle were included.Except the above methods,other therapies to the denervated skeletal muscle atrophy were excluded.RESULTS AND CONCLUSION:After peripheral nerve damage.the skeletal muscle is denervated and then inevitably atrophy occurs Therefore,the exploration of prevention and treatment of denervated skeletal muscle atrophy has attracted the interests from many domestic and foreign scholars.will certainly become the important task and the research hot spot in the 21st century in the peripheral nerve domain The microsurgery surgery,physiotherapy,biology and chemotherapeutics,gene therapy are the main effective prevention methods for the atrophy of denervated skeletal muscle.At present,this domain.s research already presents the multiple perspectives and various tendencies.The prevention and treatment of denervated skeletal muscle atrophy has already given the pointed management,but in the improvement microcycle,but much works need to carry on in fields of improving microcirculation,preventing cell apoptosis,suppressing the collagen excessive growth,as well as adjusting myogenic regulatory factors expression using gene therapy method in the gene level,but also had the.Along with the tissue engineering,cell culture science,molecular biology,genetic engineering and so on,an unceasing development will be obtained regarding the prevention and treatment of denervated skeletal muscle atrophy.

Objective To introduce an interpersonal relationship regulating technique in the static state of Qigong to enrich modern clinical psychotherapies. Methods Based on previous therapeutic experience, we introduce one specific technique of the Systematic Psychotherapy of TCM (SPT): Interpersonal relationship regulating technique in the static state of Qigong. Results Interpersonal relationship regulating technique in the static state of Qigong is a specific and practical technique of SPT, which has been gradually standardized and been mature with more than two decades of innovation and accumulation. Conclusion Interpersonal relationship regulating technique in the static state of Qigong has great clinical value.

Objective To understand the application of dietary supplements among adult patients with type 2 diabetes mellitus (T2MD) in two areas of China. Methods A total of 615 T2MD patients were recruited from three hospitals in Beijing and Shenyang, with convenience sampling for the study using self-reported questionnaires. Data were statistically analyzed. Results Overall 34. 8% of the participants (22.5% in Beijing and 45.5% in Shenyang, respectively) reported ever use of at least one dietary supplement in the past 12 months. The most commonly used supplement was royal jelly ( 16. 1% ), followed by vitamins ( 10. 1% ), calcium (8. 8% ),fish oil (7. 3% ), spirulina (3.9%), and lecithin (2. 3% ). Except that royal jelly was used for complementary treatment for T2MD, most of the other supplements were used for improving their body defense. In addition, more than 20 other health dietary supplements were also used for diabetes-related conditions in 8. 5% of the participants surveyed. The participants' main sources of information about dietary supplements were from mass media or recommendations by relatives and friends. About half of supplements users perceived that the dietary supplements were effective. The application of dietary supplements was associated with gender, educational background, living area,perceived burden of medical cost, stage of diabetes, comorbidity, and body mass index (BMI) of the patients ( all P ＜0. 05). Conclusions Dietary supplements are widely used among T2MD patients in Beijing and Shenyang.The types of these dietary supplements are diverse and lack professional instructions. It is recommended that information on dietary supplements should be included in diabetes education.

Objective:To survey the status quo of ADR occurred in our hospital. Methods:Totally 277 ADR reports were collect-ed and analyzed statistically in our hospital from January 2010 to June 2013. Results:Among the ADR cases,the ratio of male to fe-male was 1∶1. 565, and 59. 93% of ADR occurred in the people over 50 years old. Totally 240 ADR cases (86. 64%) were induced by intravenous administration and 32 ADR cases (11. 87%) were induced by oral administration. Totally 167 ADR cases were caused by antibiotics (60. 29%), among them, 54 cases wero by cephalosporins (32. 34%), 47 cases by quinolones (28. 14%) and 32 ca-ses by cephamycins (19. 16%). Totally 42 ADR cases were caused by traditional Chinese medicine injections (15. 16%), and 18 ca-ses were caused by cardiovascular system drugs (6. 5%). The main clinical manifestations were lesion of skin and its appendant organr and digestive system injury with 162 cases (58. 48%) and 62 cases (22. 38%), respectively. Conclusion: It is necessary to strengthen clinical ADR monitoring, rationally use antibiotics and emphasize the importance of safe medication of traditional Chinese medicine injections.

Objective To observe the clinical efficacy of acupuncture in treating primary hypertension and the effect of twirling reinforcing-reducing manipulations on the treatment of primary hypertension. Methods Totally 126 patients with primary hypertension were randomized into treatment group 1 of 40 cases, treatment group 2 of 44 cases, and a control group of 42 cases. The treatment groups were both intervened by acupuncture at Quchi (LI 11), while twirling reinforcing-reducing manipulations (twirling towards left) were applied in treatment group 1, while the manipulations (twirling towards right) were applied in treatment group 2;the control group was intervened by medication. The change of blood pressure after 10 min treatment was observed, and the clinical efficacies were compared. Results The total effective rate was 85.0%in treatment group 1, versus 88.6%in treatment group 2 and 61.9% in the control group, and the total effective rates in the two treatment groups were significantly different from that in the control group (P<0.05). In the two treatment groups, the systolic pressure and diastolic pressure respectively after 10 min treatment and 30 min treatment were markedly different from those 10 min before treatment in the same group (P<0.05). In the two treatment groups, the systolic pressure and diastolic pressure after 30 min treatment were markedly different from those after 10 min treatment in the same group (P<0.05). In the control group, the systolic pressure and diastolic pressure were significantly different from those 10 min before treatment in the same group (P<0.05). There were no significant inter-group differences in comparing the systolic pressure and diastolic pressure after 30 min treatment (P>0.05). Conclusions Acupuncture at Quchi (LI 11) is an effective approach in treating primary hypertension, and the reinforcing manipulation and reducing manipulation are both effective.