Objective: To investigate the influence of ischemic and pinacidil preconditioning on the activity of NF-?B and its myocardial protective effects.Methods: Eighty isolated rabbit hearts were randomized into five groups(Group C?K?I?P?G).Hearts were removed and mounted in a Langendorff apparatus perfused with 37℃ oxygenated Krebs-Henselet buffer.All groups were subjected to gobal myocardial ischemia for 40 min and reperfusion for 60 min or 120 min.Cardiac functions were monitored at 10 min equilibration ?15 min?30 min?60 min and 120 min reperfusion;Coronary flow(CF) was measured at 10 min equilibration and 30 min reperfusion;Expression of NF-?B P65 was determined at 10 min equilibration?60 min and 120min reperfusion.Results:(1)After ischemic reperfusion,the recovery of cardiac function and CF in Group I and Group P was significantly superior to that in Group C and Group K(P

This paper reports the situation of the treatment for stroke and introduces stroke unit (SU) in overseas hospitals : its important role in the emergency treatment and rehabilitation, the characteristics, advantages and managing modes. Moreover,it discusses the possibility and necessity of establishing SU with characteristics of traditional Chinese medicine.

To discuss long-term changes of refractive status in children after congenital cataract intraocular lens ( lOL) implantation and analyze its related influence factors.METHODS: Seventy cases ( 110 eyes ) with congenital cataract from January 2013 to January 2014 in our hospital were selected, according to the age of patients, they were divided into group A (<2 years old), group B (2~6 years old) , group C ( 7 ~ 12 years old ) . They were all given cataract extraction combined with lOL implantation in congenital cataract. Corneal curvature, axial length, refractive status of three groups after operation were compared, the influence factors ocular axis changes were analyzed. RESULTS: There were no statistically significant differences in corneal curvature of groups A, B, and C after operation ( P>0. 05 );The differences had statistical significances of the corneal curvature of group A after surgery and 3mo after operation (P<0. 05), there were no significant differences of the corneal curvature of group B, group C after surgery and 3mo after operation (P>0. 05);the corneal curvature 3mo after operation of group A was significantly higher than that of groups B and C (P<0. 05), there were no significant differences in the corneal curvature between group B, and group C after 3mo ( P>0. 05 ); There were no statistical significances on axial length after operation in groups A, B, and C (P>0. 05);Ocular axial length difference of group A 3mo after surgery was significantly higher than that of groups B and C ( P < 0. 05 ), ocular axial length had no statistical difference of group B, group c 3mo after surgery ( P>0. 05). Refractive status of three groups after operation showed myopia moving trend, refractive change rate and refractive diopter of group A after operation was significantly higher than that of group B, group C, the difference was statistically significant (P<0. 05). CONCLUSlON: After congenital cataract extraction combined with lOL implantation, patients present myopia refractive state trend. Performance of children below 2 years old is significant. The main factor of myopia is axial growth.

[Objective]To establish a scoring standard of operative risk assessment for the orthopaedic surgery and evaluate the reliability of it.[Method]Anivariable analysis was performed for the preoperative and intraoperative clinical data of 768 patients undergoing orthopaedic surgery from Jan.2001 to Dec.2008,the risk factors associated with orthopaedic surgery were selected.Then multivariable analysis with logistic regression was performed for the selected risk factors.The scoring standard was made through these data.Reliability of the standard was evaluated by predicting of 160 patients in spinal surgery from 2006 to 2008,comparing with the real situation of each operation.[Result]Anivariable analysis showed age,heart function,blood pressure,electrocardiogram,sternum,liver function,renal function,albumin,blood sugar,number of operative variables within four weeks,magnitude,blood loss preoperation,timing of operation were risk factors.[Conclusion]The ORAOS could appropriately predict the occurrence of patients' postoperative complication before operation.

Objective Central nervous system trauma, ischemia, hemorrhagic stroke were all experienced the same pathological damage, in related animal experiments,histone deacetylase inhibitors have a wide range of neuroprotective effect, it is expected to become a new class of potential therapeutic agents.However,the specific mechanism is not clear.In this paper, the research progress of neuroprotective effect of histone deacetylase inhibitors is reviewed,in order to improve recognition of histone deacetylase inhibitors and provide foundation for futher study.

The occurrence of breast cancer is closely related to genetic factors.BRCA1 and BRCA2,breast cancer susceptibility genes,play important roles in the occurrence and development of breast cancer.The researches on germline mutations in BRCA1 and BRCA2 are of great significance in prevention,diagnosis and treatment of breast cancer.

Objective To evaluate the dynamic MR imaging findings of peripheral cholangiocarcinoma (PCC) of the liver. Methods Seventeen patients with PCC underwent dynamic MRI during the early, middle, and delayed phase after contrast medium administration. Enhancement pattern of the tumor, the wedge-like enhancement area peripheral to the tumor, dilatation of the intrahepatic biliary ducts, vascular involvement, and extrahepatic tumor invasion were evaluated. Results Early peripheral enhancement with progressive and concentric filling by contrast medium was the most frequent enhancement pattern (9 patients). A wedge-like enhancement area was observed in 10 patients. Ductal dilatation was found in 11 patients. Vascular involvement and extrahepatic invasion were seen in 8 and 2 patients, respectively. Conclusion Enhancement pattern is relative to the character of pathology (quantity and distribution of cell and fibrosis). Early peripheral enhancement with progressive and concentric filling by contrast medium may be specific for PCC.

In recent years,gastrointestinal microbiota and fecal microbiota transplantation(FMT)has gained rapidly development.The recent studies demonstrated that FMT has obvious clinical efficacy and safety on the treatment of clostridium difficile infection,inflammatory bowel disease and other diseases in adult.The present article will discuss the efficacy of FMT in treating digestive diseases in children.This review summarizes therapeutic advances in FMT,latest FMT therapies and presents the potential of FMT therapeutics in gastrointestinal and extra-intestinal conditions in children.

Objective To explore the content of the goals set by medical freshmen. Meth-ods 189 freshmen who major in clinic medicine were asked to answer the goals they set in the period of five years colleges’life,another 100 freshmen were selected to categorize the goals with high frequencies and then factor analysis were employed.Results 46 goals with high fre-quency can be classified into 11 factors. Conclusion Although the content of the goals set by the medical freshmen included some unreasonable points,it is basically in line with that of our present goals of cultivating medical students.

BACKGROUND:Bone morphogenetic protein 9 is proved to promote the osteogenic differentiation of various kinds of stem cel s, but whether it can induce the osteogenic differentiation of dental fol icle cel s in vitro is yet unclear. OBJECTIVE:To investigate whether bone morphogenetic protein 9 can induce the osteogenic differentiation of rat dental fol icle cel s in vitro. METHODS:Purified rat dental fol icle cel s at passage 3 were transfected with bone morphogenetic protein 9 adenovirus. Then, alkaline phosphatase activity, calcium deposition and expression of osteogenesis-related factors at mRNA and protein levels were detected in the dental fol icle cel s. RESULTS AND CONCLUSION:After transfection with bone morphogenetic protein 9, the dental fol icle cel s showed continuously enhanced alkaline phosphatase activities and obviously enhanced calcium deposition. Real-time PCR results demonstrated that the mRNA expressions of alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin and core binding factor were increased significantly. The western blot assay showed that the expression of osteopontin enhanced in the dental fol icle cel s after transfection with bone morphogenetic protein 9. In summary, bone morphogenetic protein 9 can induce the osteogenic differentiation of dental fol icle cel s.