Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Radioactive 125I interstitial brachytherapy is easy to cause the intestinal tract tissue damage.Its mechanism possibly correlates to the quick renewal speed of the intestinal tract tissue and a majority of ceils in M, G2 and late S phases. The tolerant dose of intestinal tract tissue is approximately 160 Gy. If the absorbed dose is higher than the tolerant one, the intestinal tract tissue will be damaged. In the clinical practice of radio-active 125I interstitial brachytherapy, through the strict plan of the TPS system before the operation, by using ul-trasound, CT, MR/and other imaging technologies in the operating process and establishing the suitable barrier protection between the seeds and intestinal tract tissue, the radioactive damage of the intestinal tract tissue my be reduced.

Selective serotonin reuptake inhibitors (SSRIs),a new generation of anti-depressant agent,are mainly used to treat emotional disorders in clinical practice now.Recent studies have found that SSRIs can increase neurogenesis after cerebral ischemia,promote neurovascular reconstruction,and are conducive to the recovery of neurological function.Furthermore,SSRIs can improve the tolerance of ischemic tissue,exert anti-inflammation and anti-oxidation properties,reduce the blood-brain-barrier injury,and inhibit excitatory amino acid toxicity in order to exert neuroprotective effect.This article reviews the related research progress in SSRIs and ischemic stroke.

Objective To evaluate method of CT simulation (CT sim) in the treatment planning setup of esophageal carcinoma. Methods From Dec.1999 to Jun.2002, 49 patients with pathologically proved esophageal carcinoma were analyzed. All patients underwent CT sim and treatment planning was configured with three symmetrical fields in the isocenter mode. A few comparisons have been made between CT sim and conventional simulation (Con sim) in the treatment planning setup. Results The difference in isocenter position were observed between CT sim and Con sim with 3～18 mm.When the esophageal lumen was taken as portal center, the 90% isodose curve in 42.8%(21/49) of patients were able to cover the whole lesion. When taking the tumor as the portal center, the whole lesion of all patients was totally covered by the 90% isodose curve. Conclusion The CT sim has an advantage in the delineation of lateral extension of esophageal tumor than the Con sim .Selection of irradiation portal should be done according to the size the silhouette of the tumor.

BACKGROUND:There are few reports about the effect of serum-free culture on the differentiation of rat adipose-derived stem cel s into vascular endothelial cel s.
OBJECTIVE:To investigate the isolation, serum-free culture of rat adipose-derived stem cel s differentiating into vascular endothelial cel s.
METHODS:The rat adipose-derived stem cel s were isolated from male Sprague-Dawley rats and expanded to the third passage by enzymatic digestion-adherent explants method. In the experimental group, rat adipose-derived stem cel s were cultured in serum-free medium for 24 hours. In the control group, rat adipose-derived stem cel s were cultured in low-glucose Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum. After that, the cel s were cultured in inducing medium for 3 weeks to differentiate into vascular endothelial cel s.
RESULTS AND CONCLUSION:The rat adipose-derived stem cel s grew as polygonal or fusiform-shaped adherent cel s when cultured in vitro, which could stably proliferate and passage. The rat adipose-derived stem cel s showed very low expression of CD31, a cel surface marker, after passages. After directional differentiations into vascular endothelial cel s, the cel s were pebble-shaped under the inverted microscope. Expression of CD31 was up-regulated, which was much higher in the experimental group than the control group. The induced cel s in the experimental group had stronger abilities than those in the control group to swal ow Dil-labeled acetylated low-density lipoprotein and form tube-like structures on the matrigel after differentiation into vascular endothelial cel s. So, rat adipose-derived stem cel s could be highly successful y induced to differentiate into vascular endothelial cel s in vitro after serum-free culture.

Objective:To investigate the composition and clinical features of inpatients with congenital heart disease (CHD) complicated with pulmonary arterial hypertension (PAH) from a cardiovascular department.Methods: We retrospectively analyzed the clinical data of 238 PAH patients who were identified from 3 674 CHD inpatients in Changhai Hospital during Jan, 2001 to Jul, 2007.Results: Among the 3 674 CHD inpatients who were treated in our hospital in recent 6 years, 238 (6.48%) had PAH.PAH was found in 10.8% of patients with atrial septal defect (ASD), 2.81% of patients with ventricular septal defects (VSD), 5.57% of patients with patent ductus arteriosus (PDA), and 19.6% of patients with complex types.The mean age of patients was (45.6?17.7) years, with 49.6% of them being 40-60 years.Female patients accounted for 69.3% of the total.The composition of PAH patients was: ASD (62.6%), VSD(19.7%), and PDA(13.4%).The patients with VSD,PDA or other complex types were younger than those with ASD(P

Objective To observe the efficacy of Foley balloon combining with uterine adhesions to prevent the recurrence of intrauterine adhesions after resection of intrauterine adhesions.Methods Patients underwent resection of intrauterine adhesions were randomized into two groups. 30 patients in control group underwent the therapy of uterine adhesions to prevent the relapse intrauterine. On the other hand, the study group with 30 patients were placed Foley balloon for 5 ~ 7 days and combined with the therapy of uterine adhesions to intervention for 1 ~ 2 weeks. All patients were followed-up for 1 ~ 2 months, and the result of the intrauterine adhesion were assessed by hysteroscopy.Results The rate of the relapse in control group was 3.33%; the study group had the same result (the rate was 3.33%). There was no difference between these two groups.Conclusions The rate of recurrent intrauterine adhesions is no different between the control group of only use therapy of uterine adhesions and replacement of Foley balloon combining with therapy of uterine adhesions as intervention.

Adult ; Blood Cell Count ; Control Groups ; Female ; Humans ; Male ; Physicians ; Radiography, Interventional