Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective To analyze the CT features of large adrenal adenoma and to compare the pathological features of large adenoma and small adenoma in order to improve the diagnosis of large adenoma.Methods The unenhanced and enhanced CT images of 14 patients with large adrenal adenoma,proven surgically and pathologically and pathological features of small adrenal adenomas in 30 cases were retrospectively reviewed.The pathological features were evaluated in the cell composition,the incidence of cystic degeneration and hemorrhage of large adenoma and small adenoma.Results 14 large adenomas were unilateral and single,3 had distinct margin and 11 had indistinct margin,14 showed regular contour of round or ellipse,the size of tumors were 5 cm?5.5 cm～18 cm?20 cm.14 large adenomas presened heterogenous density in unenhanced CT study with CT value of-36～51 HU,and 3 showed calcification and 6 showed cystic degeneration.Most of the 14 adenomas presented heterogenenous enhancement after contrast administration.Pathologic examination indicated that the clear cells were predominant in the cell composition of small adenoma,while in the case of large adenoma,the proportion of compact cell predominance was equivalent to that of the clear cell and compact cell concomitance.3 small adenomas and 6 large adenomas had cystic degeneration,5 small adenomas and 4 large adenomas had hemorrhage respectively.Statistical analysis suggested that large adenoma had higher incidence of degeneration than that in small adenoma,while no difference was found in the cell composition and incidence of hemorrhage between large adenoma and small adenoma.Conclusion The large adrenal adenomas have higher incidence of degeneration than that in small adenoma,while no difference is found in cell composition and incidence of hemorrhage between large adenoma and small adenoma.

Objective To investigate the effect of coenzyme Q10 combined with Shenmai injection on serum enzyme in the treatment of brain hypoxic injury after asphyxia by meconium in newborn.Methods 64 cases with brain hypoxic injury after asphyxia by meconium from Medical University of Tianjin Jinghai Clinical College were selected and randomly divided into 2 groups, 32 cases in each group.The control group received maintained ventilation and circulation function and routine drug therapy adequate, and the experiment group received more with coenzyme Q10 combined with Shenmai injection for 7 days.Serum enzymes and myocardial injury markers, oxidative stress and inflammation related factors and the clinical effect and complications were compared after the treatment.Results Compared with before treatment, levels of CK-MB, AST, LDH, CK and α-HBDH decreased in two groups after the treatment, levels of CT-1, CTnI and Mb decreased, levels of SOD and MDA decreased, contents of GSH-Px, APN, IGF-1 increased, contents of Leptin decreased (P<0.05).Compared with the control group, the levels of CK-MB, AST, LDH, CK and α-HBDH in the experiment group were lower, levels of CT-1, CTnI and Mb were lower, levels of SOD and MDA were lower, contents of GSH-Px, APN, IGF-1 were higher, contents of Leptin were lower(P<0.05).The clinical curative effect rate of control group(65.63%) was lower than the experiment group (87.50%)(P<0.05).Conclusion Coenzyme Q10 combined with Shenmai injection in the treatment of brain hypoxic injury after asphyxia by meconium in newborn is curative effective with high safety, and it can reduce serum enzyme and myocardial injury.

Objective To investigate the mechanism of mizolastine inhibiting allergic contact dermatitis (ACD) in murine model. Methods The murine model of allergic contact dermatitis by topical DNFB was used. Using this model murine inhibition of ear swelling was observed after oral administration of mizolastine in different doses. The levels of IFN-?, TNF-? and IL-4 in the sera of these mice were detected by enzyme-linked immunosorbent assay (ELISA). Results Murine ear swellings were markedly suppressed in each dose group of mizolastine (P 0.05). Different doses of mizolastine inhibited the expression of these three cytokines to different degrees. Conclusion The therapeutic effect of mizolastine on murine ACD may be played by inhibiting the expression of some cytokines.

OBJECTIVE:To study the drug price administration models in some developed countries,so as to provide ref?erence information for establishing drug price administration model in China.METHODS:The comparative method and crite?rion method were adopted to study the published literature.RESULTS&CONCLUSION:Although different countries have carried out different models,they still have similarity in some policies.Therefore,we should pay attention to learn the advan?tages in different drug administration models.

Objective To discuss the importance of early di agnosis and treatment of primary hy-perparathyroidism in multiple bone resorptions.Methods From April 1997to April 2001,5patie nts were regarded as having multiple bone resorptions.There were 4females and 1male.The age ranged from 25to 46years(average 37years).The mean interval between symptom o nset and final diagnosis was 19.5mon ths(from8to 36months).All patients were suffering from se vere bone pain in need of strong antalgic,and the symptoms including fatigue,depression,loss of appetite and malaise,e tc.Abnormality on bone scan indicated multiple areas of skeletal involvem ent,bone radiographs showed multip le bone resorptions in skull,rib,pelvis and limbs.The range of serum calcium level was 2.27-3.22mmol /L and that o f PTH was 168-214ng /ml.Palpable neck mass was found i n three patients.Results All patients had undergone resectio n of the abnormal parathyroid.Three were parathyroid adenomas and two defined a s hyperplastic glands.All of them had pain relief after operation.The average follow-up was 15months(3-27months);the bone lesions have been repaired partially or totally s hown in X -ray examination during follow-up.Conclusion Clinically,in younger patients with multiple bone resorptions,hyperparathyroidism should be highly suspected,so that timely parathyroid surgery can be ca rried out for the treatment.[

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.

This research applied the theory of customer satisfaction model in the evaluation of the clinical information system. By means of qualitative and quantitative methods, it made validity and reliability measurement on the measurement tools in use. In addition, it extracted the structure validity of the factor regulating scale via a factor analysis, and developed the scale for clinical information system measurement These efforts provided the hospitals with the useful measurement tool to improve their information system, promoting positive and practical hospital informationziation.

Objective The PRESENT ( Physicians' Routine Evaluation of Safety and Efficacy of NovoMix 30 Therapy) study is a multinational clinical experience program to report the efficacy, safety and acceptability of NovoMix 30 (BlAsp 30) in patients with type 2 diabetes mellitus inadequately controlled with oral antidiabetic drugs (OADs). Methods A total of 4 754 type 2 diabetes patients inadequately controlled with oral antidiabetic drugs were enrolled in the study. All patients were prescribed BIAsp 30 in accordance with the approved labeling. Data were collected at baseline and at the end of 3 months treatment. Results The mean HbA1c, body mass index (BMI), age, and duration were (9.09±1.70)%, (24.30±2.68)kg/m2, (54.63±10.94)years, and (5.46 ±4.17) years separately. The mean daily dose was (0.43±0.14) U/kg at treatment initiation and (0.48±0.15) U/kg after 3 months treatment. After 3 months, the mean HbA1c, fasting plasma glucose (FPG) and postprandial plasma glucose (PPG) were significantly reduced from baseline levels. The mean HbA1c decreased by (2.04± 1.57)%, FPG by a (3.51±2.55)retool/L, and PPG by a (6.51±4.02) mmol/L (all P<0.01). A significant proportion (49.4%) of the patients achieved target HbA1c of ≤7%. The overall rate of hypoglycaemic episodes (events/patient-yr) decreased from 10. 098 at baseline to 3. 810 at study end. The rate of major hypoglycaemia decreased from 0. 787 to 0.126, and the rate of nocturnal hypoglycaemia decreased from 2.356 to 0.547. Compared with previous treatment, more than 99% of the patients and physicians were "satisfied" or "very satisfied" with BIAsp 30 treatment. Conclusions BIAsp 30 was found to be safe and effective to improve the glycaemia control of type 2 diabetes inadequately controlled with OADs in routine clinical practice.