Cytological characteristics of a case of adult T-cell leukemia were studied by detection of morphologic, cytochemical and immunological methods. The leukemic cells found in the peripheral blood belonged to mature T-lymphocytes and presented the changes of polymorphous nucleus which had diagnostic significance in association with clinical features and positive ATLA antibody. The mechanism of formation of cytological characteristics of adult T-cell leukemia is also discussed.

Inhibition of proliferation and induction of differentiation by 1, 25-dihydroxyvitamm D3 of human promyelocytic leukemic cell line (HL-60)are reported. At the concentration range of 109 to 106 mol/ L, 1, 25-dihydroxyvitamin D3 had effect in inhibiting cell proliferation with time-dependent and dose-dependent manner. Strong inhibition of colony cell growth (74.8?11.1%)was observed at 107 mol/L concentration. It was also found that HL-60 cells could be induced into monocytes by 1, 25-dihydroxyvitamin D3at the same concentrations,which were demonstrated by cytochemistry and specific antigen of cell surface and NBT reduction test. In addition, the relationship between proliferation and differentiation is discussed according to the result of flow cytometry.

Objective To explore the relationship of T2DM with xerophthalmia after cataract surgery.Methods Retrospective analysis was made on the clinical data of 137 cataract patients (176 eyes) from April,2016 to October,2016.The DM patients were chosen as the DM group (58 cases,71 eyes),and single cataract ones were chosen as the control group (79 cases,105 eyes).The clinical data and xerophthalmia index were compared between two groups.Results The age and sex constituent ratio of DM group and control group had no significant difference (all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those at preoperative 2 days (all P ＜ 0.01),but the indexes relieved with time prolong.The postoperative MQ score,FL,SⅠt of control group at 1 week,1 month were significantly worse than those at preoperative 2 days (P ＜ 0.01),the postoperative BUT and preoperative BUT had no significant difference (P ＞ 0.05),all of the postoperative index and preoperative index had no significant difference(all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those of the control group (all P ＜ 0.01).Conclusion The xerophthalmia after cataract surgery of T2DM patients are more seriously than normal cataract patients.

Antibodies ; immunology ; metabolism ; China ; Drug Industry ; history ; History, 20th Century ; History, 21st Century ; Immunohistochemistry ; history ; methods

HSP70 Heat-Shock Proteins ; analysis ; Hemorrhagic Fever with Renal Syndrome ; metabolism ; pathology ; Hepatitis Antigens ; analysis ; Hepatitis, Viral, Human ; immunology ; pathology ; Humans ; SARS Virus ; isolation & purification ; Severe Acute Respiratory Syndrome ; pathology ; virology

OBJECTIVE To study the classification and distribution of the ?-lactamases produced by Escherichia coli in diabetic patients.METHODS To detect the ?-lactamases produced by E.coli in diabetic patients with a multi-disk synergy test.RESULTS Forty-nine strains of 60 strains in our study produced the ?-lactamases with total positive rate of 81.67%,From them 5 strains were producing penicillinases,17 strains producing BSBLs(28.33%),and 27 strains were producing ESBLs(45.00%),no carbapenem-hydrolyzing ?-lactamases were detected.In 27 strains producing ESBLs,the sensitivities to the ceftazidime,aztreonam and cefotaxime were similar.CONCLUSIONS The types of ?-lactamases produced by E.coli in diabetic patients are mainly BSBLs and ESBLs,without carbapenem-hydrolyzing ?-lactamases;the sensitivities to the ceftazidime,aztreonam and cefotaxime are similar.

Insulin resistance and islet ? cell dysfunction are well-identified pathogenesis of type 2 diabetes. The generation of a great quantity of mitochondrial reactive oxygen species (ROS) impairs the function of mitochondria and induces oxidative stress during metabolic process of hyperglycaemia and hyperlipemia. Oxidative stress blocks insulin action pathway and induces insulin resistance; inhibit expression of insulin gene; decrease insulin secretion and impair ? cell function. In this paper, the mechanism of the ROS generation, insulin resistance and ? cell dysfunction induced by oxidative stress are reviewed.

Objective To explore the effects of dexamethasone on ovulation induction in polycystic ovary syndrome (PCOS).Methods This clinical study included 68 women with PCOS, which of them were divided into two groups: 34 in dexamethasone treating(dexamethasone combined with ovulation induction) and 34 in con-trol(ovulation induction treatment only).Subsequently, comparisons were made between pre and after drug treat-ment in two groups, the main indexes were the levels of testosterone(T), luteinizing hormone(LH), follicle stim-ulating hormone(FSH), growth rate of follicle, and body mass index(BMI).And we have also considered the o-vulation rate and pregnancy rate, and compared these indexes in dexamethasone treating group with control group.Results There were significant differences between pre and after dexamethasone treatment in the levels of T in treatment group(P < 0.01), and there were not significant differences between pre and after treatment in the lev-els of T in control group (P >0.05).LH had decreased significantly after treatment in both two groups (P <0.01), FSH had not significant differences between pre and after treatment in two groups(P >0.05).The growthrate of follicle of two groups had elevated after treatment (P < 0.01).And the ovulation rate in dexamethasone treating group was higher than that in control group(P < 0.05), but there were not significant differences between the two groups in the clinical pregnancy rates(P >0.05).There were not significant differences between pre and after treatment in the levels of BMI in both two groups (P > 0.05).Conclusions Dexamethasone can decrease the T in plasm levels.It is good for ovulation induction in PCOS.

Diabetes mellitus is a common disease of serious harmful to human health,but its pathogenesis is not entirely clear.Mitochondria are the important organelles to generate energy in eukaryocytes,and play a pivotal role in the regulation of reactive oxygen species generation,intracellular calcium homeostasis,and apoptosis signal transduction.The possible causes of mitochondrial dysfunction include oxidative stress,Ca2+ disturbances,reduction of mitochondrial biosynthesis,opening of mitochondrial permeability transition pore,mitochondrial DNA mutations,and etc..Many studies demonstrate that mitochondrial dysfunction is associated with β-cell dysfunction of type 1 diabetes mellitus and insulin resistance of type 2 diabetes mellitus.Moreover,mitochondrial dysfunction plays important roles in the development of diabetic cardiomyopathy.This present article reviewed the current status of studies on the relationship of mitochondrial dysfunction and diabetes mellitus and diabetic cardiomyopathy.It is very important to understand and study mitochondrial dysfunction and its important roles in diabetes mellitus and diabetic cardiomyopathy in order to clarify the pathogenesis of diabetes mellitus and explore new approaches of prevention and treatment for diabetes mellitus.

Objective To investigate the clinical advantages and safety profile of the combinational treatment of acute progressive cerebral infarction with aspirin,clopedogrel and atorvastatin.Methods 170 cases with acute progressive cerebral infarction were randomly divided into the treatment group and control group,85 patients in each group.Both two groups were initially treated with aspirin and atorvastatin.After excluding the possibility of hemorrhage, the treatment group additionally received clopedogrel,while the control group was treated with aspirin and atorvastatin. The difference in clinical efficacy was evaluated between before treatment and 7 days,14 days or 28 days after treatment by using the NIH Stroke Scale (NIHSS)and Barthel Index (BI).Results The NIHSS scores of the treatment group after 7d,14d and 28d were (12.52 ±3.25)points,(9.10 ±3.21)points and (6.87 ±2.85)points, which of the control group were (13.65 ±2.93)points,(10.73 ±3.41)points and (9.07 ±2.96)points respectively, the differences between the two groups were statistically significant(t =2.340,3.170,4.877,all P <0.05).The BI scores of the treatment group after 7d,14d and 28d were (35.26 ±11.18),(53.73 ±13.74)and (74.61 ±17.35), which of the control group were (31.98 ±9.12),(46.65 ±11.79 )and (63.87 ±15.73 )respectively,the differences between the two groups were statistically significant(t =2.131,3.752,4.456,all P <0.05).The overall effective rates of the treatment group after 7d,14d and 28d were 62.7%,79.5% and 94.0%,which of the control group were 51.2%,68.3% and 84.1% respectively.The differences of overall effective rates were statistically significant after 14d and 28d(χ2 =4.711,8.531,all P <0.05).Few reverse reactions were observed in both two groups.Conclusion Compared with the aspirin and atorvastatin therapy,combinational treatment of acute progressive cerebral infarction together with clopedogrel has a better efficacy,safety profile and significant promotion on neurological recovery.