OBJECTIVE:To compare the cost-effectiveness of3therapeutic schemes on acute cerebral infarction(ACI).METHODS:A total of123patients with ACI were ascribed to receive sodium ozagrel injection(shemeⅠ),ginkgo leaf extract(GLE)and dipyridamole injection(schemeⅡ)and sodium ozagrel injection plus ginkgo leaf injection(schemeⅢ),the cost-effectiveness analyses of3groups were conducted using pharmacoeconomics method.RESULTS:The costs for the3schemes were9766.51yuan,5562.22yuan and8273.05yuan,respectively;The effective rates were36.84%,32.50%and71.11%,respectively;The cost-effectiveness ratios of3were265.11,171.15and116.34,respectively.CONCLUSION:SchemeⅢis preferable among3schemes.

OBJECTIVE:To evaluate the cost-effectiveness of 3 "Cocktail" therapeutic regimens in treating acute cerebral infarction(ACI).METHODS:141 patients with ACI were administered with Sodium Ozagrel + Cinepazide + Edaravone(Group A),Vinpocetine + Propylgallate + Deproteinized calf blood Extractives(Group B)or Sodium Ferulate + Buflomedil + Muscular Amino Acids and Peptides and Nucleosides(Group C)for 14d.Cost-effectiveness analysis in pharmacoeconomics was applied to analyze the therapeutic effects and costs.RESULTS:The total costs of 3 groups(A,B and C)were 5 970.67 yuan,4 865.11 yuan and 3 939.72 yuan,respectively,the efficiency rates were 82.98%,63.04% and 64.58% respectively,and the cost-effectiveness ratio were 7 195.31,7 717.50 and 6 100.53,respectively.CONCLUSION:Group A is preferable for ACI.

Cervical spinal canal tumors is not rare,accounting for about 15%of all spinal tumors. Due to the particularity of its anatomical location and the severity of operative complications, it is considered as a difficult point in spinal surgery. With the development of imaging medicine and various surgical techniques,many new theories and techniques have been developed. Its overall treatment effect is satisfactory, but the serious surgical complications not rare. This article reviews the progress in treatment of cervical spinal canal tumors, in order to provide a reference for the further improvement of cervical spinal canal tumors treatment .

Objective: To verify 5-HT4 receptor mRNA expression in intestinal mucosa mast cells (IMMC). Methods: IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT4 receptor mRNA in IMMC. Results: Evidently positive staining was shown in the cytoplasm of IMMC. Conclusion: We have verified the expression of 5-HT4 receptor mRNA in IMMC for the first time and provided evidence for further research.

Objective To study the effect of comprehensive care on the level of high-sensitivity Creactive protein and matrix metaloproteinase-9 in patients with hemorrhagic infarction.Methods 48 patients with hemorrhagic infarction were divided into the control group and the observation group with 24cases in each group.The control group was given conventional care,the observation group was given comprehensive care.Serum hsCRP and MMP-9 level of all patients were measured on day 1,day 3,day 7,day 14.Results The level of serum hsCRP and MMP-9 in the observation group on day 7 was significantly decreased compared with on day 1.The level of serum hsCRP and MMP-9 in the observation group on day 7 was lower than the control group.On day 14,the level of serum hsCRP and MMP-9 returned to normal in the observation group,which was lower than the control group.Conluusions The implementation of comprehensive care for HI patients can effectively reduce the hsCRP and MMP-9 level in serum,shorten hospitalization time,and is conducive to the prognosis of patients.

BACKGROUND: Reproductive activity of hepatocytes is limited. There are numerous studies concerning stem cells differentiation into hepatocytes, including embryonic stem cells, bone marrow cells, pancreas stem cells, neural stem cells, various sources of mesenchymal stem cells.OBJECTIVE: To explore the possibility of induction of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) into hepatocyte-like cells in vitro culture.DESIGN, TIME AND SETTING: A cytological in vitro study was performed at the Institute of Hematology, Medical College of Jinan University from October 2005 to April 2006.MATERIALS: Fetus cord blood was obtained from spontaneous delivery and caesarean delivered healthy pregnant women at the Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University. The parturients signed the informed consents.METHODS: UCB-MSCs were incubated in vitro, and digested in trypsin-EDTA. The third passage of cells at 5 × 10~4 cells/cm~2 wereinoculated. Original medium was removed 48 hours later. Cells were washed in phosphate-buffered saline. In the first phase, cells were incubated in F12 medium supplemented with dexamethasone, hepatocyte growth factor, epidermal growth factor and 1 ×its for 2 weeks. In the second phase, cells were incubated in F12 medium containing dexamethasone, hepatocyte growth factor,oncostatin M and 1 × ITS for two weeks.MAIN OUTCOME MEASURES: The following parameters were measured: expression of surface marker of UCB-MSCs using flow cytometry, expression of related gene and protein of hepatocytes following induction respectively using RT-PCR and immunofluorescence staining.RESULTS: No CD34/CD45/CD14 of hematopoietic markers were detected, either no the CD54, CD49f, HLA-DR were found. The low expression of CD106 and high expression of CD29, CD44, CD13 were found. The gene expression of a-fetoprotein, albumin,CK-18 and TAT were discovered, and three kind of protein a-fetoprotein, albumin, CK-18 were positively observed in cytoplasm after 4 weeks of induction using immunofluorescence staining.CONCLUSION: UCB-MSCs are able to differentiate into hepatocyte-like cells in vitro culture following combination with many growth differentiation factors, such as dexamethasone, hepatocyte growth factor, epidermal growth factor, tumorigenesis M and ITS.

AIM: To explore the possibility of differentiating functional insulin-producing cells from human BM-derived stem cells. METHODS: Mesenchymal stem cells were isolated from human bone marrow. Then these cells were induced with epidermal growth factor, β-mercaptoethanol and high concentration of glucose. The gene expression related to islet β cells was detected by RT-PCR. Insulin in the treated cells was examined by immunocytochemistry. In addition, the levels of insulin secretion and glucose-stimulated insulin release were examined by microparticle enzyme immunoassay. Finally, the induced cells were implanted into the right renal subcapsular space of diabetic mice. Blood glucose levels were monitored 16 d after implantation. The right kidneys of the treated mice were harvested for immunohistochemistry. RESULTS: The key genes related to pancreatic β cells had been confirmed to express by PCR and insulin was detected by immunocytochemistry in differentiated human BM-derived stem cells induced by high glucose, which responded to glucose challenge. Furthermore, implantation of the cells in renal subcapsular space was able to lower the glucose levels in hyperglycemic mice. After 16 days, the implanted cells were determined still to be insulin positive cells by immunohistochemistry. CONCLUSION: These results indicate human BM-derived stem cells are capable of differentiating into functional insulin-producing cells and may represent a pool of cells for the treatment of diabetes.

Objective To study the effect of formaldehyde exposure on DNA-protein crosslinks (DPC) in buccal mucosa cells in students who were taking anatomy course.Methods The modified SDS-KCl precipitation assay published by Zhitkovich and Costa in 1992 was applied to detect DNA-protein crosslinks in human cells.And this method has been used to explore DPC induced by different pollutants in numerous studies.37 medical students (20 males and 17 females) aged 19.24?1.09 (mean?standard deviation) and 40 students (20 males and 20 females) in natural science college aged 19.55?0.99 (mean?standard deviation) were studied.The frequency of exposure to formaldehyde was 6 h per week in exposed group.Results Concentrations of formaldehyde in anatomy laboratory ranged from 0.42 to 1.57 mg/m3.Exposure to formaldehyde resulted in an increase of DNA-protein crosslinks.The percentage (mean?standard deviation) of DNA-protein crosslinks in exposed and nonexposed students were 25.72%?6.48% and 22.88%?5.34% respectively(P0.05).Conclusion The result of the present study suggests that formaldehyde exposure in medical students increases the frequency of DNA-protein crosslinks in buccal mucosa cells and females may be more sensitive to formaldehyde exposure.

Objective To explore the relationship of T2DM with xerophthalmia after cataract surgery.Methods Retrospective analysis was made on the clinical data of 137 cataract patients (176 eyes) from April,2016 to October,2016.The DM patients were chosen as the DM group (58 cases,71 eyes),and single cataract ones were chosen as the control group (79 cases,105 eyes).The clinical data and xerophthalmia index were compared between two groups.Results The age and sex constituent ratio of DM group and control group had no significant difference (all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those at preoperative 2 days (all P ＜ 0.01),but the indexes relieved with time prolong.The postoperative MQ score,FL,SⅠt of control group at 1 week,1 month were significantly worse than those at preoperative 2 days (P ＜ 0.01),the postoperative BUT and preoperative BUT had no significant difference (P ＞ 0.05),all of the postoperative index and preoperative index had no significant difference(all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those of the control group (all P ＜ 0.01).Conclusion The xerophthalmia after cataract surgery of T2DM patients are more seriously than normal cataract patients.

Objects To observe whether mesenchymal stem cells exist in umbilical cord blood and if they can differentiate into osteoblasts and adipocytes.Methods Nucleated cells were separated with Hespan,Mesenchymal-like stem cells were identified by surface marker with flow cytometry.Osteoblast was identified by von Kossa staining and alkaline phosphatase staining,adipocyte was identified by Oil Red O staining.Results From the Nucleated fraction of UCB,we demonstrated the presence of a subset of cells that express adhesion molecules CD13+,CD29+,and CD44+,but not antigens of hematopoietic CD34,CD45,CD14 and neither antigens of endothelia CD106.Exposure of these cells to osteogenic inductive agents resulted in an increase in the expression of alkaline phosphatase and the appearance of hydroxyapatite nodules by Von Kossa staining.Incubation with adipogenic inductive agents resulted in morphological change and positive staining with oil Red O.Conclusions Mesenchymal-like stem cells exist in cord blood and have a lower precursor frequency.The cells can differentiate into osteoblast and adipocyte.Cord blood may function as a new source of cells for cellular therapeutics.