After being introduced to Japan, the Chinese acupuncture and massage therapy has changed a lot, in which the influence aroused by the blind practitioners cannot be ignored. Through analyzing the development and the transition of the blind acupuncture and massage industry in Japan, it is found that the tube needle technique, changeable acupoints concept, technical deviation and the importance on acupoints rather than meridians are still existed commonly today, which are introduced by the blind acupuncture and massage practitioners, the special group in Japan. In the process of development, the interaction with the governmental strategy has played the essential role in the consolidation of the above features.
Acupuncture ; education ; history ; manpower ; Acupuncture Points ; Acupuncture Therapy ; history ; History, 15th Century ; History, 16th Century ; History, 17th Century ; History, 18th Century ; History, 19th Century ; History, Ancient ; History, Medieval ; Humans ; Japan ; Massage ; history ; manpower ; Visually Impaired Persons

Objective To detect the levels of Th1 and Th2 type cytokines of peripheral blood CD+4 T lymphocytes in the patients with cervical cancer in order to find out the laboratory evidence of tumor immunotherapy. Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance expression of cytokines. Then specific fluorochrome-conjugated monoclonal antibody was added to be combined with its specific antigen. Results The level of IL-2 produced by Th1 cells were lower in patients of cervical cancer than those of normal controls, there were significant difference between them. But the level of IFN-? produced by Th1 cells had no significant change in patients of cervical cancer in comparison with normal controls. The levels of Th2 type cytokines such as interleukin-4(IL-4), interleukin-6(IL-6), interleukin-10(IL-10) were higher in patients of cervical cancer than those of normal controls, there were significant difference between them. Conclusion Th1/Th2 is imbalance in cervical cancer .Th1 type cytokines are inhibited, and Th2 type cytokines are relatively enhanced, so Th1/Th2 shifts to Th2. It may be the mechanism of tumor arising and transferring by immune escape from immunosurveillance.

Objective To observe the effect of Diosmin Tablets about the prevention and treatment of edema that breaks out after a circular mixed hemorrhoids operation and the role of fibrosis on edema.Methods Having 120 cases of circular mixed hemorrhoid patients, Acco 60 cases were randomly divided into treatment group and the others were in comparison group.Both groups were outside the cut line of circular mixed hemorrhoid ligation within the injection, after these operations do conventional anti-inflammatory, hemostasia and local Ma Ying Long musk hemorrhoids ointment medication.The next day of the operation, the treatment group take a dose of Diosmin tablets 1.5 g bid 4 d, then changing the dose 1 g bid 3 d.ResultsThe incidence of edema, treatment group was 6.6% and the other group was 30%.edema in the incidence of the two groups were significantly different(P ＜ 0.01).The treatment group non-fibrosis, but the other group have 4 patients getting fibrosis.The two sets of rate about edema fibrosis were significantly different(P ＜ 0.01).Conclusion Diosmin Tablets can effectively do prevent and disperse circular mixed hemorrhoid postoperative edema.

0.05. Conclusion: The suppressive effect of MSC on patients with ITP in vitro showed no difference from that on the controls.

High separation efficiency and fast separation speed of liquid chromatography(LC), strong sensitivity and good selectivity of mass spectrometry technology, have realized the wide use of LC and mass spectrometry technology in the field of biological macromolecules. In recent years, LC and mass spectrometry technology are gradually becoming universal in the field of vaccines, and play an increasingly important role in the quality control of vaccines. This paper summarized the application of LC and mass spectrometry technology in inactivated vaccines, vaccines of virus-like particles(VLPs), polysaccharide vaccines, and polysaccharide conjugate vaccines.

BACKGROUND:Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells. OBJECTIVE:To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media. METHODS:A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of“mesenchymal (stromal) stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium”in English and Chinese, respectively. Final y, 41 articles were retained. RESULTS AND CONCLUSION:Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages;therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.

BACKGROUND:Platelet lysate has been known as a kind of lysate of autologous or alogeneic platelet-rich products. It not only removes the residual cel structure, reduces immunogenicity, but also retains many growth factors. Platelet lysate has been suggested as a substitute for fetal bovine serum to expand mesenchymal stem cels in vitro. OBJECTIVE:To observe the effects of platelet lysate on biological characteristics of human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels, and provide some experimental data for clinical cel therapy and regenerative medicine. METHODS:Platelet lysate was prepared by repeated freezing and thawing from fresh blood. Healthy adult bone marrow and adipose tissue were colected. Human bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were obtained by density gradient centrifugation and type I colagenase digestion. We tested the morphology, cel phenotype, differentiation characteristics, proliferation capacity, colony forming ability and the level of cytokine secretion of bone marrow mesenchymal stem cels and adipose mesenchymal stem cels after cultured with platelet lysis. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cels and adipose mesenchymal stem cels were successfuly cultured in vitro using platelet lysate. There were no significant differences in morphology, cel phenotype, colony forming ability and the level of cytokine secretion, and chondrogenic, osteogenic and adipogenic capacities between bone marrow mesenchymal stem cels and adipose mesenchymal stem cels. Adipose mesenchymal stem cels had a high cumulative population doublings than bone marrow mesenchymal stem cels (P < 0.05). These findings suggest adipose mesenchymal stem cels had a stronger proliferative ability, and are more suitable for large-scale expansionin vitro cultivation system of platelet lysate compared with bone marrow mesenchymal stem cels.

Humans ; Thyroid Neoplasms ; pathology ; therapy

OBJECTIVE:To evaluate the application status of narcotic analgesics in the inpatients of our hospital.METH-ODS:The prescriptions of narcotic analgesics of inpatients in 2005 were analyzed statistically in respect of the total number of prescriptions,name and specification of drugs,dosage and administration,duration of medication,total consumption quantity,and medication purpose etc with defined daily dose(DDD) and drug utilization index(DUI) as indexes.RESULTS & CONCLUSION:The DUI of all the five narcotic analgesics used in our hospital were ≤1.00,which is in line with the principle of Three-step Analgesic Ladder Approach for Caner Pain recommended by WTO.

Objective To investigate the influencing factors of hematoma enlargement after spontaneous intracerebral hemorrhage. Methods The patients with spontaneous intracerebral hemorrhage were enrolled retrospectively in the study. The demographics, vascular risk factors, and baseline clinical data of the patients were collected. Hematoma enlargement was defined as CT scan within 48 h showed that the increased hematoma volume was more than >1/3 of the baseline. The demographics, vascular risk factors, and baseline clinical data of the hematoma enlargement group and the non-hematoma enlargement group were compared. Multivariate logistic regression analysis was use to identify the risk factors for hematoma enlargement. Results A total of 121 patients with spontaneous intracerebral hemorrhage were enrolled, including 69 males and 52 females, their ages were 24 to 89 years (mean 59.16 ±12.68). Forty-five patients (37.2%) had combined hypertension, 5 (4.1%) had diabetes, and 11 (9.1%) had hyperlipoidemia. Thirty patients (24.79%) had hematoma enlargement within 48 h. The time from ictus to the first CT scan was 4.21 ±1.57 h and the time from ictus to CT scan again was 34.78 ±6.90 h. Univariate analysis showed that the proportion of patients drinking alcohol (46.7% vs.26.4%; χ2 =4.313, P =0.038), as well as systolic blood pressure (174.53 ±20.02 mmHg vs.160.63 ±19.79 mmHg, t = -3.327, P = 0.001; 1 mmHg = 0.133 kPa), fasting blood glucose (7.67[6.70-9.47]mmol/L vs.6.78[5.81- 7.79]mmol/L; Z = -2.266, P =0.023), National Institutes of Health Stroke Scale (NIHSS) score (10.00[8.00-12.00] vs.5.00[3.00-8.00]; Z = -5.468, P < 0.001) in the hematoma enlargement group were significantly higher than those in the non-hematoma enlargement group, while low-density lipoprotein cholesterol (2.60[2.10- 2.91]mmol/L vs.3.00[2.60-3.41]mmol/L; Z = -3.905, P <0.001) was significantly lower than that in the nonhematoma enlargement group. Multivariate logistic regression analysis showed that baseline systolic blood pressure (odds ratio [OR] 1.036, 95% confidence interval [CI] 1.007-1.065; P =0.014) and NIHSS score (OR 1.310, 95% CI 1.111-1.544; P =0.001) were the independent risk factors for hematoma enlargement, while low –density lipoprotein cholesterol (OR 0.279, 95% CI 10.105-0.742; P =0.011) was the independent protective factor for hematoma enlargement. Conclusions Systolic blood pressure and NIHSS score were the independent risk factors for hematoma enlargement after spontaneous intracerebral hemorrhage, while low -density lipoprotein cholesterol was the independent protective factor for hematoma enlargement.