Since the endotoxic fever is mediated essentially by endogenous pyrogen (EP), we have examined the possibility that the central cAMP is involved in the mechanism of biphasic fever induced by endotoxin, as in the case of biphasic fever induced by EP. The results showed that administration of endotoxin in rabbits elicited typical biphasic fever. The fluctuation of concentration of cAMP in cerebrospinal fluid (CSF) paralleled with the change of the rectal temperature, similar to that of the bipbasic fever caused by EP. Raised level of cAMP in plasma was observed only in the first phase. The cAMP concentration in CSF correlated positively with the febrile response. The correlation of cAMP levels in CSF and plasma was observed only in the first phase but not in all the febrilic periods. These findings indicated that the mechanism of endotoxic biphasic fever is associated with the Change of central cAMP. Content. The raised level of cAMP in CSF during fever is likely to represent the biosynthsis in brain regions. However, the possibility that the raised cAMP in plasma enters CSF in the first phase is not excluded.

The signals of body temperature from 4 different febrile patterns were sampled for power spectral analysis. The results showed that the spectrums had no difference between their distribution in frequency domain, only a low frequency peak has been seen. The values of the power spectrum varied directly to the intensity of the febrile responses. A high degree of relationship was found between the power spectral density and thermal response index (TRI).

AIM: To study the mechanism of the inhibitory effect of human polymorphonuclear leukocytes (PMN) on the production of tumor necrosis factor-? (TNF-?) in U937 cells. METHODS: PMA differentiated U937 cells were cocultured with PMN in the presence of lipopolysaccharide. Sodium azide was added in the culture. The supernatants of cell culture were collected and TNF-? was assayed with ELISA. RESULTS:The inhibitory effect of PMN on the production of TNF-? in U937 cells was observed. Sodium azide restored the TNF-? production in U937 cells/PMN coculture to the extent of 45% in concentration of about 10～15 ?mol/L. These concentrations of sodium azide did not affect TNF-? production by U937 cells. The restoration effect of sodium azide on PMN inhibition was not related to blocking of myeloperoxidase (MPO) activity. The MPO inhibitors 3-amino-1,2,4,-triazole and 4-aminobenzoyl hydrazide had few effect on PMN inhibition. CONCLUSION: These results indicated that PMN inhibition depended on the cell-cell interactions. Sodium azide may be a useful reagent for further studying this mechanism.

A new method of calculating fractal dimension of short-term heart rate variability signals is presented. The method is based on wavelet transform and filter banks. The implementation of the method is: First of all we pick-up the fractal component from HRV signals using wavelet transform. Next, we estimate the power spectrum distribution of fractal component using auto-regressive model, and we estimate parameter 7 using the least square method. Finally according to formula D = 2- (gamma-1)/2 estimate fractal dimension of HRV signal. To validate the stability and reliability of the proposed method, using fractional brown movement simulate 24 fractal signals that fractal value is 1.6 to validate, the result shows that the method has stability and reliability.
Algorithms ; Electrocardiography ; Fourier Analysis ; Fractals ; Heart Rate ; physiology ; Humans ; Least-Squares Analysis ; Nonlinear Dynamics ; Signal Processing, Computer-Assisted

ObjectiveTo explore the role of psychological capital in psychological stress and mental maladjustment in recruits.Methods405 recruits were sampled and investigated using the psychological stress selfevaluation test( PSET),the psychological capital questionnaires(PCQ),and military mental maladjustment scale (MMMS).ResultsPsychological stress had significant positive correlation with each factor of mental maladjustment ( r =0.164 ～ 0.438,P ＜ 0.01 ),and psychological capital had significant negative correlation with each factor of mental maladjustment ( r=-0.312 ～ -0.463,P＜ 0.001 ).The hierarchical regression analysis indicated that psychological capital,confidence,hoping,resiliency and optimism had the positive prediction of all the four factors of mental maladjustment,the variance contribution rates were 27.2％,20.1％,25.8％ and 15.9％,respectively; the main effect of psychological capital(β1 =-0.406,P＜0.01 ;β2 =-0.351,P＜0.01 ) and psychological stress(β1 =0.304,P ＜ 0.01 ;β2 =0.267,P ＜ 0.01 ) on recruits'behavioral problem and interpersonal relationship maladjustment was significant,the interaction effect of psychological capital and psychological stress on recruits' behavioral problem (β =- 0.098,P ＜ 0.05 ) and interpersonal relationship maladjustment (β =- 0.087,P ＜ 0.05 ) was significant.ConclusionPsychological capital is a moderator in interaction between mental maladjustment and psychological stress.The PCQ score may predict the variation of recruits'mental status.

Objective To study the association of SNP of the AdipoR1 gene with T2DM in Xi’an population. Methods The amplification refractory mutation system(ARMS) analysis and gene sequencing were used to investigate the AdipoR1 gene polymorphism in 100 type 2 diabetics and 84 normal control subjects. Results (1) The genotype and allele frequencies of -106A/G, 5843A/G were not significantly different between type 2 diabetics and normal control subjects. (2) The diagnosis age of diabetics was significantly younger in AdipoR1 5843GG genotype group than in other genotype groups. Conclusions The data implicate that the AdipoR1 gene -106A/G and 5843A/G polymorphism may be not associated with pathogenesis of T2DM. AdipoR1 5843 GG genotype may be associated with the earlier diagnosis of T2DM

The antagonistic effects of ruthenium red (RuR) on the biological activi-ties of endotoxin (ET) which included the limulus amebocytes lysate test, the local Shw-artzman reaction, decreasing the white blood cell counts, and increase of zinc in periphe-ral blood were observed. The destroyed meshes of endotoxin have been seen under electronmicroscope. The amino-groups of endotoxin increased markedly, which it was treated withRuR. These results indicated that the polycationic residues of RuR may be the effectiveunits of antiendotoxin activity.

OBJECTIVE:To discuss the adjustment of patients'medication psychology in clinical pharmaceutical care.METHODS:Various factors that influence patients'medication psychology and the effects resulted from patients'medication psychology were analyzed.RESULTS&CONCLUSIONS:Patients'psychological effects influence the physiological effects of medicine,thus greatly influencing drug action,therefore,pharmacists'knowledge on pharmacopsychology should be strength?ened and their communication ability with patients should be improved.Furthermore,pharmacists should adjust patients'medication psychology timely to satisfy their basic psychological needs for medication.

The bystander effect(BE) plays an important role in the gene therapy of cancer by the herpes thymidine kinase/ganciclovir(HSV-TK/GCV) system.It enhances the therapeutic efficacy of this system.Up to now,the exact underlying mechanism of the bystander effect remains unclear.A large body of evidence has indicated a close correlation of the connexin expression and gap junction in the targeted cells to the bystander effect.Here the publications concerning the relationship of gap junction with the bystander effect in the HSV-TK/GCV treatment have been reviewed.The possible cell death signals that can be transferred through gap junction to induce the bystander effect are also discussed.

Objective To explore the clinical features,diagnosis and treatment of solid-pseudopapillary tumor(SPT)of pancreas.Methods The clinical data of 19 patients with SPT treated in 8 years were studied retrospectively,included 3 males and 16 females.Results The type of operation was decided by the size and location of tumor.Pancreatic leakage was a familiar complication,there were 9 cases.Patients were followed up from 3 to 70 months,and no recurrence or metastsis was found.Conclusion Pancreas SPT is a rare type neoplasm of pancreas.Pancreas SPT is affecting primarily young women,complete resection results in excellent prognosis.