Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Animals ; Cattle ; Humans ; Infant ; Infant Formula ; Milk Hypersensitivity ; Milk Proteins ; adverse effects

Objective To understand the difference of Demodex prevalence among medical students living and studying together with different classes, sexes and nationalities. \ Methods\ Demodex folliculorum and Demodex brevis were detected by using cellophane tape on the nasolabial grooves. \ Results\ The overall prevalence was 51\^5% in 2 248 medical students. Prevalence in different classes and sexes was: freshman 42\^6%, sophomore 49\^6% and third year students 66\^8%; 55\^7% in males and 48\^7% in females. Prevalence in students with different nationalities was: Mongolian 49\^8%, Han 52\^8% and other minorities 64\^3%. \ Conclusion\ The Demodex prevalence in students of senior classes was higher than those from junior ones (P

Volume overload or hidden lung congestion is emerging as a pervasive problem in end-stage renal disease (ESRD) patients on hemodialysis,which will lead to cardiovascular complications and impact the patients' life quality and survival time,so how to improve volume control in patients on hemodialysis is a urgent research hotspot.Recently,the ap plication of lung ultrasound for semi-quantitative assessment pulmonary congestion in patients on hemodialysis has received general concern.Lung ultrasound not only can detect excessive extravascular lung water in the early period of pulmonary congestion,but also can predict cardiac events and mortality in ESRD.Lung ultrasound is an effective,sensitive,real-time and non-invasive tool beside.The application and progresses of lung ultrasound in evaluation of pulmonary congestion in hemodialysis patients were reviewed in this article.

Objective To compare the clinical features of myocardial infarction (MI) in young adults with and without diabetes. Methods A total of 94 young adults with MI were enrolled in this study and divided into two groups according to whether they had diabetes:diabetic group (DM) and Non diabetic group (NDM).The clinical features,angiographic results,risk factors and prognosis were compared between the two groups. Results The incidence of atypical chest pain (25.0% vs 7.6%),non-ST segment elevation myocardial infarction (50.0% vs 9.1%),ventricular wall motion abnormalities (78.6% vs 42.4%),double vessels (35.7% vs 21.2%),triple vessels (39.3% vs 3.1%) and the incidence of mortality(10.7% vs 1.5%)were all significantly higher in DM group than in NDM group (P<0.05 or P<0.01).The number of affected vessels and Gensini scores were (2.110±0.875) and (52.61±10.47) in DM group,and(1.140±0.677),(34.02±10.24) in NDM group.The between group difference was statistically significant (P<0.05).Multiple linear regression showed that HbA1c and smoking were the independent risk factors for Gensini score. Conclusion The clinical features of young adult patients with MI and diabetes are more complex,their coronary stenosis are more serious,and the prognosis is poor.Smoking and HbA1c are positively associated with Gensini scores.

Objective To investigate the effects of 1,25-dihydroxyvitamin D3 in the prevention of corneal allograft rejection. Methods Forty-five SD rats (only right eyes) were used as recipients,and 15 Wistar rats (double eyes) were used as donors. SD rats were randomly divided into 3 groups: the autograft control group,the allograft control group (receiving peanut oil as placebo),and the allograft group were treated with 1,25-dihydroxyvitamin D3 (1.0 ?g.kg-1.d-1,3 times/d for 13 d,i.p.). Drugs were injected intraperitoneally for 14 d after transplantation. Routine antibiotic eye drops were used. Grafts were observed by operating microscopy everyday after transplantation. Five SD rats in each group were killed respectively at the 14,21,and 30 d postoperatively. Neovascularization and inflammation were evaluated with HE staining. ELISA was used to detect the contents of IL-1?,IL-2,IL-8,and IL-10 in the peripheral blood. Results The mean survival time (MST) of the graft was (21.7?6.8) d in the autograft control group,(11.2?2.5) d in the allograft control group,and (19.3?5.2) d in the allograft group. There was statistically significance between the latter 2 groups (P

Objective To study the effect of letrozole (LE) on ovulation in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty-four patients with PCOS were divided into LE treatment group (n=68) and chloramiphene (CC) treatment group (n=96).Growth rate of follicles,serum hormone level,endometrial thickness,and cervix mucus were observed before and after treatment.Results The growth rate of follicles was 1.67 and 1.78 mm/d,respectively,in LE and CC treatment groups (P=0.14).The serum T level on the day of LH peak was 2.1?1.9 and 2.0?1.7ng/dL,respectively,in LE and CC treatment groups (P=0.93).The serum testosterone (T) level on day of LH peak and on day 3 of menses was 2.1?1.9 and 1.4?1.5 ng/d,respectively,in LE and CC treatment groups (P=0.09).The serum E2 level on day of LH peak was 89.0?59.5 and 108.1?46.0 pg/ml,respectively,in LE and CC treatment groups (P=0.13).The endometrial thickness was 11.3?1.6 and 9.7?1.4 mm,respectively,in LE and CC treatment groups (P=0.07).The score of cervix mucus was 11.0?1.1 and 8.3?3.1,respectively,in LE and CC treatment groups (P=0.003).Conclusion Letrozole can effectively induce ovulation in patients with polycystic ovary syndrome,and does not increase serum T level.

Objective To explore the relationship between calcium channel ?1 subunit (Cav1.1) gene intron 26 -67 A/G polymorphism and thyrotoxic periodic paralysis(TPP). Methods Cav1.1 gene polymorphism at position -67 was determined by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) in 46 male patients with TPP, 68 male Graves’ disease (GD) patients without TPP and 72 healthy male controls. The difference of genotype and the variation of allele frequencies were analyzed by Chi-square test. Results (1) Frequencies distribution of AG+GG genotype in TPP, GD and control groups were 47.83%, 14.71% and 29.17% respectively, and those of allele G were 44.57%, 13.24% and 27.78% for the three groups respectively. (2) Frequencies of -67 AG+GG genotype in TPP group were significantly higher than those in GD and CON group(OR=5.32, P

Objective To investigate the changes of protein C and fibrinolytic system in patients with acute cerebrovasculer disease and the influence of intervened treatments on acute cerebral infarction(ACI).Methods Determinations of protein C(PC),protein S (PS),thrombomodulin(TM),Tissue-type plasminogen activater(t-PA),plasminogen activator inhabitor(PAI) were performed in patiens with acute cerebrovasculer disease and normal control(NC),then redeterminated these items after treatments in patients with ACI.Results PC,PS:The levels in patients with ACI were significantly lower than that in other groups.After dreatments,the levels in patients with ACI increased.There were no significantly discrepancy in acute cerebral hemorrhage(ACH) and lacunar cerebral infarct(LCI) compared with NC;TM:The levels in patients with ACH,ACI were significantly higher than that with LCI and NC.After dreatments,the levels in patients with ACI decreased;t-PA:The levels in patients with ACI were lower than NC,whereas the levels in patients with ACH increased.After dreatments,the levels in patients with ACI increased;PAI:The levels in patients with ACI were significantly higher than NC,and also found that the levels in patients with ACH and LCH were higher compared with NC.The levels in patients with ACI decreased after dreatments.Conclusions There are obviously abnormalities in plasma coagulative and fibrinlytic parameters in acute cerebrovasculer disease;To check the levels of PC,PS,TM,t-PA,PAI are important to predict the type of stroke,the seriousness of disease,the effect of treatment,prognosis of the cases and suggest that earlier treatments be important.

The effects of monoterpene perilly alcohol (POH) alone or in combination with STI571 on the proliferation and apoptosis of the cell line K562 positive for Bcr/Abl were investigated. By using cell culture, the effect of the drugs on the proliferation of the cells was studied. TUNEL and flow cytometry assay of FITC-Annexin V and PI labeled cells were applied to detect the effects of the drugs on the apoptosis of the cells. The results showed that at 36 h, IC50 of POH on K562 positive for Bcr/Abl and HL-60 negative for Bcr/Abl were 81.0 +/- 11.3 micromol/L and 113.6 +/- 23.4 micromol/L respectively (P>0.05). POH could inhibit the proliferation of K562 in a time- and dose-dependent manner with the inhibitory rate of 100 micromol/L POH on K562 cells at 36 h being (53.2 +/- 3.65)%. K562 cells were more sensitive to STI571 than POH. IC50 of STI571 on K562 cells in 36 h was (0.256 +/- 0.054) micromol/L. In a time- and dose-dependent manner, POH induced the apoptosis of K562 cells with the percentage of apoptotic cells by 100 micromol/L POH at 40 h being (21.0 +/- 3.3)%. Both 100 micromo/L POH and 0.2 micromol/L STI571 had the same inhibitory effects on the K562 cells at 36 h. But at 12 and 24 h, the inhibitory rate of POH was significantly higher than that of STI571 (P<0.05) and the ability of STI571 inducing apoptosis at 36 h was greater than that of POH. 50 micromol/L, 100 micromol/L and 200 micromol/L POH in combination with 0.2 micromol/L STI571 could obviously increase the inhibitory effects on the cellular proliferation. Combined use of 50 micromol/L, 100 micromol/L, 200 micromol/L with 0.2 micromol/L STI571 could strongly induced apoptosis, especially 200 micromol/L POH in combination with 0.2 micromol/L STI571. It was concluded that the antileukemia effect of POH had no obvious Bcr/Abl positive selectivity. POH can inhibit the proliferation of K562 and induce the apoptosis in a time- and dose-dependent manner. K562 cells were more sensitive to STI571 than POH. POH in combination with STI571 could obviously enhance the abilities of STI571 inhibiting the proliferation and inducing apoptosis of K562 cells.
Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Dose-Response Relationship, Drug ; Drug Synergism ; Fusion Proteins, bcr-abl/analysis ; HL-60 Cells ; K562 Cells ; Monoterpenes/*pharmacology ; Piperazines/pharmacology ; Pyrimidines/*pharmacology