Objective: To verify 5-HT4 receptor mRNA expression in intestinal mucosa mast cells (IMMC). Methods: IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT4 receptor mRNA in IMMC. Results: Evidently positive staining was shown in the cytoplasm of IMMC. Conclusion: We have verified the expression of 5-HT4 receptor mRNA in IMMC for the first time and provided evidence for further research.

OBJECTIVE:To evaluate the cost-effectiveness of 3 "Cocktail" therapeutic regimens in treating acute cerebral infarction(ACI).METHODS:141 patients with ACI were administered with Sodium Ozagrel + Cinepazide + Edaravone(Group A),Vinpocetine + Propylgallate + Deproteinized calf blood Extractives(Group B)or Sodium Ferulate + Buflomedil + Muscular Amino Acids and Peptides and Nucleosides(Group C)for 14d.Cost-effectiveness analysis in pharmacoeconomics was applied to analyze the therapeutic effects and costs.RESULTS:The total costs of 3 groups(A,B and C)were 5 970.67 yuan,4 865.11 yuan and 3 939.72 yuan,respectively,the efficiency rates were 82.98%,63.04% and 64.58% respectively,and the cost-effectiveness ratio were 7 195.31,7 717.50 and 6 100.53,respectively.CONCLUSION:Group A is preferable for ACI.

OBJECTIVE:To compare the cost-effectiveness of3therapeutic schemes on acute cerebral infarction(ACI).METHODS:A total of123patients with ACI were ascribed to receive sodium ozagrel injection(shemeⅠ),ginkgo leaf extract(GLE)and dipyridamole injection(schemeⅡ)and sodium ozagrel injection plus ginkgo leaf injection(schemeⅢ),the cost-effectiveness analyses of3groups were conducted using pharmacoeconomics method.RESULTS:The costs for the3schemes were9766.51yuan,5562.22yuan and8273.05yuan,respectively;The effective rates were36.84%,32.50%and71.11%,respectively;The cost-effectiveness ratios of3were265.11,171.15and116.34,respectively.CONCLUSION:SchemeⅢis preferable among3schemes.

Cervical spinal canal tumors is not rare,accounting for about 15%of all spinal tumors. Due to the particularity of its anatomical location and the severity of operative complications, it is considered as a difficult point in spinal surgery. With the development of imaging medicine and various surgical techniques,many new theories and techniques have been developed. Its overall treatment effect is satisfactory, but the serious surgical complications not rare. This article reviews the progress in treatment of cervical spinal canal tumors, in order to provide a reference for the further improvement of cervical spinal canal tumors treatment .

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blood Vessels ; injuries ; Child ; Child, Preschool ; Female ; Free Tissue Flaps ; blood supply ; Head ; surgery ; Humans ; Male ; Middle Aged ; Neck ; surgery ; Tissue Transplantation ; adverse effects ; methods ; Young Adult

Humans ; Lymph Nodes ; pathology ; surgery ; Neck Dissection ; methods ; Oropharyngeal Neoplasms ; pathology ; surgery

Endophthalmitis ; complications ; drug therapy ; microbiology ; Humans ; Infant, Newborn ; Meningoencephalitis ; complications ; drug therapy ; microbiology ; Suppuration ; complications

Objective To study the influence of partial restraint stress (PRS) on visceral sensitivity and plasma 5-hydroxytryptamine (5-HT) concentration in rats and to study the effects of 5-HT 4 receptor agonist and antagonist on visceral sensitivity and plasma 5-HT level in rats under PRS.Methods Male Wistar rats (n=32) were divided into four test groups. After two hours of PRS or sham-stress, 1-methyl-2-pyrrolidinone (solvent), tegaserod(a partial 5-HT 4 receptor agonist), and GR113808(a 5-HT 4 receptor antagonist) were administered intraperitoneally to the corresponding groups respectively. Thirty minutes after intraperitoneal injection, visceral sensitivity of rats, which was reflected by abdominal cramps induced by rectal distension, was recorded. Rectal distension was performed by insertion and inflation of a balloon(0.4-1.2 ml). Abdominal contractions were recorded by electromyography. Plasma 5-HT concentration was determined by fluorometry. Results Stress enhanced the number of abdominal cramps induced by rectal distension compared with sham-stress for all volumes of distension (0.4 ml:10.00?3.74 vs. 6.57?1.40; 0.8 ml: 16.75?2.92 vs.11.86?3.44; 1.2 ml: 19.50?4.24 vs. 14.86?3.19,P

Objective To study the effect of formaldehyde exposure on DNA-protein crosslinks (DPC) in buccal mucosa cells in students who were taking anatomy course.Methods The modified SDS-KCl precipitation assay published by Zhitkovich and Costa in 1992 was applied to detect DNA-protein crosslinks in human cells.And this method has been used to explore DPC induced by different pollutants in numerous studies.37 medical students (20 males and 17 females) aged 19.24?1.09 (mean?standard deviation) and 40 students (20 males and 20 females) in natural science college aged 19.55?0.99 (mean?standard deviation) were studied.The frequency of exposure to formaldehyde was 6 h per week in exposed group.Results Concentrations of formaldehyde in anatomy laboratory ranged from 0.42 to 1.57 mg/m3.Exposure to formaldehyde resulted in an increase of DNA-protein crosslinks.The percentage (mean?standard deviation) of DNA-protein crosslinks in exposed and nonexposed students were 25.72%?6.48% and 22.88%?5.34% respectively(P0.05).Conclusion The result of the present study suggests that formaldehyde exposure in medical students increases the frequency of DNA-protein crosslinks in buccal mucosa cells and females may be more sensitive to formaldehyde exposure.

?AlM:To investigate the sensitive parameters of the anterior chamber changes with Pentacam anterior segment analysis system before and after laser peripheral iridectomy (LPl) in primary angle-closure suspetive (PACS).
? METHODS: Sixty eyes of 33 PACS patients were enrolled in this study. Pentacam examination was performed before and 1d after LPl to measure the central anterior chamber depth ( CACD ) , the peripheral anterior chamber depth ( PACD ) , the anterior chamber volume ( ACV) and the peripheral anterior chamber angle ( ACA) . Statistical analysis used paired t test.
?RESULTS: There was no statistical significance on the changes of ACD. PACD and ACV increased significantly between before and 1d after LPl. ACA was widened from (22. 26o±5. 18o) to (26. 42o±5. 20o), which were increased significantly between before and 1d after LPl.
?CONCLUSlON: LPl can deepen the PACD and increase the ACV in PACS. PACD and ACV are the sensitive parameters of the anterior chamber changes with Pentacam anterior segment analysis system.