Objective To asses the clinical applied value for the salpingian recanalization by means of interventional procedure.Methods 80 cases ranged in age from 23～35 with sterility diagnosed by uterosalpingography were included in this study.They had bilateral and unilateral salpingian narrow,adhesion or stenosis.According to the interventional radiologic theory,using same-axis catheter system,and recanalization fluid the narrow,stenosis and adhesion of salpingian were treated.Results There were 113 salpingians suffered with narrow,adhesion and stenosis in 80 cases.Of them,87 salpingians were recanalized.The successful rate was 84.5%,among them the recanalization rate was 94.8% in the patients with interstitial lesion and 25 cases had been pregnancy (approximate 31.9%) after operation. Conclusion The recanalization of salpingian by interventional radiology in the treatment of sterility caused by salpingian stenosis,narrow and adhesion is an effective means.The pregnancy rate is relevant to the selection of indication.

Objective To compare the chemical components among Rhizoma Alismatis of different specifications. Methods Rhizoma Alismatis of 8 different weights were chosen, and then contents of 23-acetate alisol B were determined by HPLC, and infrared spectrometry fingerprint was determined by Fourier transform infrared spectroscopy. Results The contents of 23-acetate alisol B in Rhizoma Alismatis of 8 different specifications were over 0.06%, and had no relation with specification of Rhizoma Alismatis (P>0.05). The similarities of infrared spectrometry fingerprint were above 0.9. Conclusion The chemical components among Rhizoma Alismatis of different specifications were basically the same. Contents of 23-acetate alisol B of Rhizoma Alismatis of 8 different specifications conformed to regulation of China Pharmacopoeia.

Objective To observed the expression of serum TNF-α and IL-10 in rats with severe acute pancreatitis (SAP) at different altitudes,and to explore the relationships between TNF-α and IL-10,the pathological changes of the pancreas,and the experimental basis for clinical diagnosis and treatment of SAP.Methods 72 specific pathogen free (SPF) Wistar male rats were divided randomly into three groups:1 500 meters altitude (group L),3 300 meters altitude (group M),and 4 300 meters altitude (group H).These three groups were then each divided randomly into four subgroups:control (group n),6 hours after pancreatitis (group p 6 h),12 hours after pancreatitis (group p 12 h),and 24 hours after pancreatitis (group p 24 h).Pancreatitis was induced by intraductal administration of 5％ sodium taurocholate hydrate (NaTc).The rats were killed at 6,12,and 24 hours after NaTc injection in groups p.The group n rats were killed after 6 hours of pancreas observation.Blood samples and pancreatic tissues were collected post mortem and enzyme-linked immunosorbent assay (ELISA) measured serum TNF-α and IL-10.Results Compared with the control (group n),histopathological scores,IL-10,and TNF-α in the same altitude had a significant difference (P ＜ 0.05) in group p at each time point.In the same altitude of group p,histopathological scores and IL-10 were increased with time elapsed (P ＜ 0.05),while TNF-α was decreased with time elapsed (P ＜ 0.05).There was a significant difference between group Mp and Lp in histopathological scores,IL-10,and TNF-α (P ＜ 0.05),and the same result between group Hp and Lp (P ＜ 0.05),but there was no significant difference between group Hp and Mp (P ＜ 0.05).Meanwhile,IL-10 had a positive relationship with histopathological score,but TNF-α had a negative relationship with histopathological score.Conclusions The level of TNF-α increased with increasing altitude but significantly reduced with elapsed time.The level of IL-10 increased with both increasing altitude elapsed time.These results suggested that TNF-o and IL-10 might play a important role at different times in severe acute pancreatitis.

Objective To investigate the relationship between serum anticardiolipin antibody-immune globulin G (ACA-IgG),interleukin-10 (IL-10 ),IL-17 levels and intracranial large-artery atherosclerotic stenosis in patients with ischemic stroke. Methods From March 2014 to March 2015,a total of 176 consecutive patients with the first-ever ischemic stroke admitted to the Department of Neurology,Liaocheng People′s Hospital,Shandong Province,China,and performed DSA were enrolled prospectively. Seven of the patients with cardiogenic embolism,5 with moyamoya disease,8 with arteritis,2 with artery dissection,9 with autoimmune diseases or acute and chronic inflammation were excluded,21 with extracranial arterial stenosis were not enrolled,and finally 124 were enrolled in the study. According to the findings of DSA,the degrees of intracranial large artery stenosis were divided into a stenosis-free group (n = 34),a mild-stenosis group (n = 30),a moderate-stenosis group (n = 32),and a severe-stenosis group (n = 28). The differences of serum ACA-IgG,IL-10,IL-17 levels and baseline factors of the 4 groups were compared,and multivariate logistic regression analysis was used to analyze several factors that affected intracranial large-artery stenosis. Results There were no significant differences in sex,age,alcohol consumption rate,smoking rate,and incidence of hyperlipidemia among the 4 groups of patients (all P >0. 05). Compared with the stenosis-free group,there were significant differences in the incidences of hypertension and diabetes among the mild-stenosis,moderate-stenosis and severe-stenosis groups (the incidence of hypertension,80. 0% [n = 24],93. 8% [n = 30],89. 3% [n = 25]vs. 55. 9% [19 cases];χ2 = 8. 271,8. 920,and 10. 877,respectively;P = 0. 038,0. 032,and 0. 014,respectively). The incidences of diabetes were 33. 3% (n = 10),43. 8% (n = 14),60. 7% (n = 17)vs. 8. 8% (n = 3),(χ2 = 7. 960, 8. 733,and 9. 285,respectively;P = 0. 043,0. 035,and 0. 027,respectively). Incidence of diabetes of the severe-stenosis group was higher than that of the mild-stenosis group (χ2 = 9. 348,P = 0. 025). There were no significant differences in the incidences of hypertension and diabetes among other groups (all P >0. 05). There were significant differences in ACA-IgG levels (23 ± 5,39 ± 8,51 ± 9,and 65 ± 10 kU/ L);IL-10 levels (108 ± 33,85 ± 25,77 ± 21,and 62 ± 19 ng/ L),and IL-17 levels (38 ± 10,58 ± 22,63 ± 31, and 75 ± 26 ng/ L)among the stenosis-free,mild,moderate and severe-stenosis groups (F = 17. 754,9. 827, and 12. 656;respectively;all P < 0. 01). Compared with the stenosis-free group,the ACA-IgG and IL-17 levels of the patients in the mild,morderate,and severe stenosis groups increased significantly (ACA-IgG level:t =2. 307,2. 559,and 3. 374,respectively;P = 0. 026,0. 014,and 0. 001,respectively,the IL-17 levels:t =2. 183,2. 549 and 3. 159,respectively;P = 0. 037,0. 013,and 0. 002,respectively),while the IL-10 level decreased significantly. There were significant differences among the groups (t = 2. 036,2. 351,and 2. 762, respectively;P = 0. 042,0. 023,and 0. 006,respectively). Compared with the mild-stenosis group,the ACA-IgG and IL-17 levels of the severe stenosis group increased significantly (t = 3. 154 and 2. 976 respectively;P = 0. 002 and 0. 004 respectively). There were no significant differences among the pairwise comparisons of other groups (P >0. 05). The results of logistic regression analysis showed that hypertension, diabetes,ACA-IgG level,and IL-17 level were the risk factors for intracranial large-artery stenosis (OR, 3. 043,95% CI 1. 606 -5. 875,P = 0. 003;OR,2. 912,95% CI 1. 513 -5. 824,P < 0. 01;OR,1. 837,95% CI 2. 057-3. 416,P = 0. 037;OR,1. 453,95% CI 1. 346 -2. 721,P = 0. 014). Conclusion ACA-IgG and IL-17 may play an important role in the occurrence and development processes of intracranial large-artery atherosclerotic stenosis.

Objective To study the diagnostic value of contrast-enhanced CT scan with split bolus for the ureleropelvic junction obstruction(UPJO) caused by aberrant vessel of kidney.Methods 52 patients with UPJO underwent contrast-enhanced CT scan with split bolus.The aberrant vessels and its origin as well as the relationship between aberrant vessels and UPJO were observed comparatively with the results of surgery.Results In 52 cases,CT showed UPJO caused by aberrant vessl of kidney in 14 cases,including accessory renal arteries in 10,ovarian artery originated from left renal artery in one,right accessory renal artery in one and gonad veins in 2.CT findings were corresponded to operations in all these 14 cases.The sensitivity and specificity of CT in evaluating UPJO were 100% respectively.Conclusion The UPJO caused by aberrant vessel of kidney can be diagnosed accurately by contrast-enhanced CT scan with split bolus,which can be offered for surgical treatment for UPJO.The patient's CT dose can be decreased with split bolus.

Objective To investigate the effects of periostin (Postn) on oxidized low density lipoprotein (ox-LDL)-induced injury in human artery endothelial cells (HAECs) and its underlying mechanisms.Methods The HAECs were randomly divided into 4 groups:control group,ox-LDL group,Postn siRNA group and negative siRNA group.The mRNA and protein expression was analyzed by RT-qPCR and Westem blot respectively.Cell proliferation was tested by MTT.Cell apoptosis was determined by flow cytometry.NF-κB DNA binding ability was measured by EMSA.Results Compared with control group,the mRNA and protein levels of Postn were increased significantly (P＜0.05);the ability of cell proliferation was reduced (P＜0.05);the cell apoptosis rate was increased (P＜ 0.05);the protein expression levels of VCAM1,ICAM1,E-selectin,IL-1β,IL-6,TNF-α,p65 and p-IκB-α were significantly up-regulated (P＜0.05),and the NF-κB DNA binding ability was markedly increased (P＜ 0.05) in ox-LDL group,which were all reversed in Postn siRNA group.Conclusions Postn siRNA transfection may reduce ox-LDL-induced endothelial cell injury,which may be related with the inhibition of NF-κB signaling pathway.

Objective To explore the effect of total saponins from Medicago polymorpha (TSMP) on glucose metabolism in type 2 diabetes mellitus (T2DM) rats and its possible mechanism.Methods T2DM rats were established by feeding with fat diets and intraperitoneally injecting with STZ 30 mg/kg.The rats were divided into control group,model group,metformin group (0.2 g/kg),TSMP high dose group (1.4 g/kg) and TSMP low dose group (0.7 g/kg),which were administrated for four weeks.At the end of administration,blood samples were collected to determine insulin resistance index (IRI),levels of fasting blood glucose (FBG),glycosylated hemoglobin (HbAlc),hepatic glycogen,interleukin (IL)-1 β,tumor necrosis factor (TNF)-α,free fatty acids (FFA) and Leptin,and activities of pyruvate kinase (PK),hexokinase (HK),glucose-6-phosphatase (G-6-Pase),fructose-1,6-bisphosphatase and glucokinase (GK).Western blot was used to detect the expression levels of G-6-Pase and phosphoenolpyruvate carboxykinase (PEPCK) proteins.Results Compared withthe model group,high dose (1.4 g/kg) TSMP decreased IRI,levels of FBG,HbA1 c,IL-1β,TNF-α,FFA and Leptin,and activities of G-6-Pase and fructose-1,6-bisphosphatase in T2DM rats (P＜ 0.05),while increased level of hepatic glycogen,and activities of PK,HK and GK (P＜0.05).Moreover,high dose (1.4 g/kg) TSMP down-regulated expression levels of G-6-Pase and PEPCK protein in liver tissues.Conclusion Chronic administration of TSMP can improved glucose homeostasis in T2DM rats,which might be related to promoting utilization of glucose,and alleviating inflammatory and insulin resistance.

Gallstone is a common and frequent disease and frequent incidence, secondary infection and cancer seriously affect the health of patients. Academic organizations in different regions have issued multiple guidelines and consensus to promote the normative diagnosis and treatment of gallstones. However, in clinical practice, most symptomatic gallstones are treated, while the formation and prevention process of gallstones are ignored, making the concept of treating without a disease has not been strengthened.This article reviews the risk factors and mechanisms of gallstone formation, and points out the importance of effective prevention during stone formation. In the stage of gallstone formation, the high risk factors of stone formation can be analyzed through two aspects of injury factors and protective factors, and the high risk groups of stone formation can be screened out. According to the pathophysiological progression of gallstones, personalized prevention and follow-up strategies can be developed for the stone formation stage of gallstones.

Objective? To investigate the childbirth experience of vaginal delivery and provide a theoretical basis for improving the quality of midwifery services. Methods? From August to December in 2017, puerpera were selected from a ClassⅢ Grade A obstetrics and gynecology hospital in Beijing and a ClassⅢ Grade A general hospital using the convenient sampling method. General information questionnaire and Childbirth Experience Questionnaire 2.0 (CEQ 2.0) were used to survey the participants within 72 hours after delivery. SPSS 23.0 was used for analysis. A total of 250 questionnaires were distributed, and 240 valid questionnaires were returned. The response rate was 96.0%. Results? The total score of childbirth experience was (78.30±9.21), with perceived safety score of (17.52±3.21), participation score of (12.06±2.24), professional support score of (16.21±2.0), own capability score of(20.44±3.47) . There was a statistically significant impact of expectation of childbirth method, being multipara, taking prenatal course, understanding of vaginal birth, fear or anxiety during delivery on the total score of childbirth experiences (P＜ 0.05). Conclusions? Overall, the childbirth experience is good. Hospital staff should promote prenatal courses, provide maternity knowledge for patients, foster better understanding of delivery process, help establish appropriate childbirth expectations, reduce fear and anxiety associated with childbirth, and improve the childbirth experience.

Objective? To understand the attitude and behavioral status quo of obstetric nursing staff in caring for maternal women with fetal loss, so as to provide effective reference for the establishment of obstetric grief nursing model. Methods? By convenience sampling, nurses and midwives working in the obstetrics department in a ClassⅢ Grade A obstetrics and gynecology hospital of Beijing from March 1st to 31st of 2017 were selected. Self-designed "Questionnaire on Attitude and Behavior of Obstetric Nurses on Grief Nursing" was used to investigate the attitude and behaviour status of the obstetric nurses and midwives on grief nursing. The questionnaires were distributed collected by 2 charge nurses in obstetric department. The data were analyzed using SPSS 23.0 software. Results? A total of 165 questionnaires were distributed and 165 ones were retrieved. The score of grief nursing attitudes of obstetric ward nurses was (37.03±7.01), and the score of grief nursing behaviors was (27.08±5.61); the score of grief nursing attitudes of obstetric ward midwives was (39.15±7.22), and the score of grief nursing behaviors was (16.76±4.51).There were significant differences in the scores of grief nursing attitudes among nurses of different ages, working years and professional titles (P＜ 0.05); there were statistical difference in scores of grief nursing behaviors from nurses with different working years and professional titles(P＜0.05). The highest score of grief nursing attitudes was (4.25±0.85), and the lowest was (2.81±1.05); the highest score of grief behaviors was (4.33±0.98), and the lowest was (1.79±1.04). Conclusions? Obstetric nurses' perception of grief nursing is relatively limited and lack of standardized obstetric grief nursing mode. It is necessary to further improve the education and training of sensitive culture of medical staff in grief nursing consultation. A standardized grief nursing model should be established according to the psychological status and actual psychological needs of the mothers with lost fetuses in China.