OBJECTIVE:To establish a method for the content determination of L(-)-Anabasine in different medicinal parts (stem,rhizome,fibrous root,rootlet) of Alangium chinense,and provide reference for its development and utilization. METH-ODS:HPLC was performed on the column of Thermo C18 with mobile phase of methanol-phosphate buffer solution(22∶78,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 259 nm,column temperature was 30 ℃;the injection volume was 10 μl. RESULTS:The linear range of L(-)-Anabasine was 0.020 62-0.257 80μg(r=0. 999 9);the limit of quantitation was 1.7 ng,limit of detection was 0.5 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.38%-98.86%(RSD=0.6%,n=6). The content of L(-)-Anabasine in different medicinal parts was the fibrous root>the rootlet>the rhizome>the stem. CONCLUSIONS:The method is simple,accurate,reproducible and specific,and suitable for the content determination of L(-)-Anabasine in different medicinal parts(stem,rhizome,fibrous root,rootlet)of A. Chinense.

Objective To explore identification method for genuine glabrous greenbrier rhizome and ensure an accurate clinical medication. Methods Such aspects as characters, microscope appearance, physicochemical analysis,chemical composition, and efficacy were used to identified the genuine glabrous from its adulterants. Results There are significant differences between glabrous greenbrier and its adulterants in the above-mentioned aspects. Conclusion Genuine glabrous greenbrier rhizome can be identified since it has obvious differences from its adulterants.

Contrast induced nephropathy is an important complication arising from use of iodinated contrast media.Such iatrogenic complication may result in poor prognosis and additional health care costs.Cases of contrast induced nephropathy are on the rise along with the-increasing number of contrast-requiring procedures.Contrast induced nephropathy has been a subject of concern to cardiologists in recent years.Several large series have shown no approved methods for management of periprocedural contrast induced nephropathy.However,identification of high-risk patients,improvement and rectification of related risk factors,use of iodinated contrast media with less renal toxicity,and sufficient hydration may reduce the incidence of contrast induced nephropathy.

External quality assessment (EQA)as an effective tool for quality control can help clinical laboratories to discover quality problems during testing process and prompt clinical laboratories to improve their testing quality.Samples used for traditional EQA frequently have matrix effects which assign the target value by grouping.Ideal samples for EQA are com-mutable which have the same numeric relationship between different measurement procedures as that expected for patients’ samples.And they can assess accuracy against a reference measurement procedure or a designated comparison method.When analyzing,robust statistical method can be used to reflect the variability of testing results between laboratories more truly. And the evaluation standards should be desirable.If unsatisfactory results appear,we should investigate every aspect of tes-ting process,find the root cause,and take corrective action.On condition that no official EQA scheme is launched for some analytes,it is necessary for us to evaluate the performance of analytes by alternative evaluation procedures.

Objective To study the internal quality control(IQC)designing procedure of the quantitative immunoassay testing i-tem to provide the most suitable IQC rule for the clinical laboratory immunoassay.Methods 3 quantitative detection items of thy-roxine,cortisol and thyroid stimulating hormone were taken for examples.The coefficient of variation(CV)of the method was ob-tained by the repeatability test for evaluating the methodological imprecision.Inaccuracy(bias)was obtained by the methods com-parison.The accuracy of the method was evaluated.Then the most suitable IQC rule for each quantitative detection item was select-ed by plotting the location of the operating point on the normalized operational process specifications(OPSpecs)chart according to CV and bias.Results Two multirule methods of 1 3s/(2of3)2s/R4s/3 1s/6 x and 1 3s/(2of3)2s/R4s/3 1s were identified when the oper-ating point was plotted on the normalized OPSpecs chart for N =6 and 90% analytical quality assurance(AQA)at the thyroxine de-cisive level of 0.064 4 mmol/L.When the cortisol decisive level was 0.735 μmol/L,the normalized OPSpecs chart for N =3 with 90%AQA showed the three solutions of 1 3s/(2of3)2s/R4s/3 1s ,1 2 .5s ,and 1 3s/(2of3)2s/R4s as for thyroid stimulating hormone,3 1s/2 2s/R4s/41s multirole procedure was the best choice for N =4,50%AQA at the upper control level,and the N =2 chart with 50%AQA showed a multirole procedure with 1 3s/2 2s/R4s at the lower control level.Conclusion The suitable internal quality control de-signing procedure of the quantitative immunoassay testing item can be selected by using the OPSpecs chart.

BACKGROUND: There are currently few studies regarding the pharmacokinetics of vancomycin via intravitreous injection.OBJECTIVE: To determine the concentration of vancomycin injected into the vitreous chamber of conscious rabbits.METHODS: A microdialysis probe was implanted into vitreous chamber of normal rabbit eyes and rabbit eyes infected withbacterial endophthalmitis for 24 hours, and 10 g/L vancomycin 0.1 mL was administered intravitreally. The drug concentration inthe vitreous chamber of rabbit eyes was determined at 0.5, 1, 2, 4, 6, 12, 24, 48, 72 and 84 hours after injection, through themicrodialysis and high performance liquid chromatogram-ultraviolet detection.RESULTS AND CONCLUSION: The metabolism of vancomycin showed an open two-compartment model in normal rabbit eyes.Its half-life was 51.66 hours and the peak concentration was 695.92 mg/L. The metabolism of vancomycin in the infected vitreouschamber showed a one-compartment model. Its half-life was 11.91 hours and the peak concentration was 713.35 mg/L. All rabbitswere injected with drugs for 84 hours and the intravitreous concentration of vancomycin was higher than minimal inhibitoryconcentration. The experimental findings indicate that microdialysis coupled to high performance liquid chromatography is apowerful tool to investigate the ocular pharmacokinetics of vancomycin, and the samples are harvested in a real-time, continuousand dynamic fashion when the experimental animals are conscious.

80mmHg in all patients at stimulating test. Selected septal branch angiography was taken. The methods of target septal branch selection included balloon occlusion test, angiography through the catheter lumen, and ultrasonic echocardiogram monitor. The 1st septal branch was ablated in 6 patients, and in 2nd branch in 2. The volume of alcohol injected was 1 75~7 0ml. The results showed that the successful rate of PTSMA operation was 100%, and the clinical symptoms for all patients improved significantly with mean LVOTPG decreased by 68 3%?13 0% at the end of the procedure. No severe ventricular arrhythmia occurred. One patient was followed-up clinically and angiographically half a year after the operation and his LVOTPG was 0 mmHg and the NYHA heart function was raised to Ⅱ grade from Ⅳ grades before operation. PTSMA is a promising interventional technique for HOCM therapy with satisfactory short-term clinical outcome.

〔Abstract〕 Taking North Sichuan Medical College as an example, the paper analyzes the current situation and problems in computer network basis and application teaching.Combining with teaching practice, according to the characteristics of medical students, it elabo-rates teaching reform which involves teaching idea, plans, contents and methods, etc.

Haloacetic acids are the main disinfection by-products in chlorinated drinking water,which are known or suspected to carcinogenic. Many countries have promulgated haloacetic acids as the routine monitoring item for drinking water safety. In order to better control the formation of haloacetic acids in chlorinated drinking water,many techniques and analysis methods were exploited. This review summarized the development of techniques and analysis methods and the comparative analysis was conducted in technique cost,method detection limit,sensitivity,resistance to disturb and sample pretreatment. The electrospray mass spectrometry is the best choice,but the instrument and operation cost is much higher,so the standard method based on the gas chromatography will still be the popular method for drinking water quality analysis.

Objective To observe the influence of telephone follow-up and home follow-up on compliance behavior and activities of daily living (ADL) of stroke patients during rehabilitation. Methods 50 cases were as telephone follow-up group and 50 cases were as home follow-up group extracted from 355 stroke patients who discharged the hospital during August 1, 2010 to July 1, 2012. They were followed up 2 weeks, 1 month and 3 months after discharge respectively. Their ADL were assessed with Bathel index (BI) at discharge and 3 months after discharge, and the compliance behavior were assessed 3 months after discharge. Results There was no significant difference in the score of BI between 2 groups at discharge (P>0.05), and the score of BI improved in both groups 3 months after discharge (P<0.05), but it was higher in the home follow-up group than in the telephone follow-up group (P<0.05), as well as the rate of compliance behavior (P<0.01). Conclusion Regular home follow-up can improve the compliance behavior of stroke patients during rehabilitation.