Objective To explore identification method for genuine glabrous greenbrier rhizome and ensure an accurate clinical medication. Methods Such aspects as characters, microscope appearance, physicochemical analysis,chemical composition, and efficacy were used to identified the genuine glabrous from its adulterants. Results There are significant differences between glabrous greenbrier and its adulterants in the above-mentioned aspects. Conclusion Genuine glabrous greenbrier rhizome can be identified since it has obvious differences from its adulterants.

OBJECTIVE:To explore optimal regime and less ADR for lupus nephritis(LN) by adopting self-made TCM prescription.METHODS:109 LN patients were assigned into control group(n=48) and therapy group(n=61).Both groups were treated with metacortandracin and cyclophosphamide.Control group were additionally given self-made TCM prescription.The clinical efficacy and adverse drug reaction(ADR) of 2 groups were compared.RESULTS:The curative rate and total response rate of therapy group were 77.05%(47/61) and 95.08%(58/61) respectively and those of control group 58.33%(28/48) and 77.08%(37/48).The curative rate and total response rate of therapy group were all higher than those of control group.There were significant difference between 2 groups(P

0.05),as well as in the composite of MACCE(2.51% vs 3.10%,P=0.523)between the two groups.Also,no significant difference existed in the rate of SAT(0.16% vs 0.16%,P=1.000)and hemorrhagic events(2.51% vs 2.12%,P=0.652)between the two groups.Kaplan-Meier survival analysis showed the cumulative hazard rate for the MACCE was similar(P=0.523).Conclusions Short-term therapeutic effects of atorvastatin 20mg/d combined with clopidogrel for the patients undergoing coronary stenting is similar to that of provastatin combined with clopidogrel.Long-term therapeutic efficiency and safety of the two strategies need to be further investigated.

Objective To establish a continuous flow analytical method for determination of potassium in environmental water sample.Methods The concentration of patassium in environmental water sample was determined by ALLIANCE auto-matic analyzer,and its data were compared with the data obtained from atomic absorption spectrophotometry.Results The de-tection limit of this method was0.5?g /ml.The method revealed linear relationship at the potassium concentrations of5-1000?g /ml.The recovery rate of standard addition method ranged from to97%to102%.The relative standard deviations were0.82%-1.23%.Conclusion The contents of potassium in environmental water samples could be accurately and rapidly deter-mined by continuous flow analysis.

Objective By investigating the quality and unreasonable prescription of traditional Chinese medicine in our hospital, to analyze the reason and thus put forward the solution and elevate the whole rational administration level in our hospital. Methods 3000 prescriptions prescribed in our hospital from April 2008 to April 2009 were randomly investigated. Select the unreasonable prescriptions and unreasonable use of medicines in a prescription for statistical analysis. Results There were 258 unreasonable prescriptions among spot-check 3000 sheets of prescriptions, reaching 8.6%. There were 633 unreasonable medications, including oversized or excessively small dosage, improper compatibility, repeated medications, etc. Conclusion There is the phenomena of unreasonable prescription in our hospital. Therefore, we should reinforce the management of prescribing medicines and enhance the level of reasonable medication.

Objective To investigate the application of nasal jejunal tube and nasogastric tube of two different catheter ways in improving the severe craniocerebral injury and the low temperature condition and the role of early enteral nutrition support and compare the difference between its curative effect.Methods A total of 120 patients with severe craniocerebral injury underwent normal and low temperature treatment were selected,and they were divided into nasal jejunal tube enteral nutrition(nasal jejunal tube group,60 cases)and nasogastric tube enteral nutrition(nasogastric tube group,60 cases)by simple random sampling.After implement nasogastric tube and nasal jejunal tube,normal pressure using enteral nutrition elemental diet and/or enteral nutrition multi polymerization agent produced the best nutrient solution implementation of early enteral nutrition support,two groups of subjects under the condition of bowel function exists,within 48 h starting enteral nutrition,compared two groups of various nutrition indicators as well as adverse reactions and complications,and Glasgow Coma Scale(GCS)score,Acute Physiology and Chronic Health Evaluation (APACHE Ⅱ)score,etc.Results The patients with severe craniocerebral injury after early nutrition,the various nutrition indicators had improved in two groups.The rise extent of triceps skinfold thickness,arm muscle circumference,serum albumin,prealbumin,transferrin,hemoglobin and other indicators in nasal jejunal tube group was higher than that in nasogastric tube group on the 14th,28th day after admission,and there was significant difference(P ＜ 0.05).The incidence of diarrhea,bloating,vomiting,and stomach contents reflux retention in nasal jejunal tube group was lower than that in nasogastric tube group [5.0％(3/60)vs.18.3％(11/60),10.0％(6/60)vs.21.7％(13/60),5.0％(3/60)vs.21.7％(13/60),15.0％(9/60)vs.16.7％(10/60),11.7％(7/60)vs.48.3％(29/60),8.3％(5/60)vs.21.7％(13/60)],and there was significant difference(P ＜ 0.05).The incidence of complications in nasal jejunal tube group was lower than that in nasogastric tube group [16.7％(10/60)vs.76.7％(46/60)],and there was significant difference(P ＜0.05).The changes of GCS score,APACHE score in nasal jejunal tube group was more obvious than that in nasogastric tube group,and there was significant difference(P ＜ 0.05).Conclusions Early nasal jejunal tube enteral nutrition for the severe craniocerebral injury after the low temperature condition in clinical enteral nutrition implementation is feasible and reasonable,compared with a nasogastric tube feeding,can obviously reduce the complications of enteral nutrition and reach goals earlier nutritional therapy,can improve the prognosis.

Objective The aim of this investigation is to explore the effects of hypoxia on the biological activity of endothelial progenitor cells(EPC)and to improve the efficacy of EPC transplantation.Methods Rat bone marrow derived EPC were isolated and cultured either under normoxic or hypoxic conditions.The proliferation,migration and angiogenic ability of EPC were observed.Results In hypoxic group,the number of attached cells per high power field(hpf)was significantly more than that in normoxic group (91.0?8.0)vs(42.5?5.3),P

Objective To compare the one-year outcome between complete and incomplete revascularization by percutaneous coronary intervention (PCI) in patients with multivessel coronary disease. Methods From June 1995 to September 2004, a total of 2579 patients with multivessel coronary disease were treated by PCI in our centre, among them 2278 patients (88.3%) achieved complete revascularization (CR group) and 301 patients achieved incomplete revascularization (IR group). One-year outcome was compared between the two groups. Results The rates of triple vessel disease, complex type B2/C lesions, chronic total occlusion were significantly higher in IR group than those in CR group, and the target vessel stenostic degree before PCI was also more severe in IR group. The PCI success rates in CR group and IR group were 96.4% and 94.0% (P0.05). The rates of angina recurrence and major adverse cardiac events (MACE) were significantly lower in CR group patients compared with the patients in IR group (5.7% vs 9.2%, P

Objective To observe the effectiveness and safety of clopidogrel combined with fibrinolytic therapy for acute ST segment elevation myocardial infarction(STEMI).Methods From January 2004 to December 2006,a total of 158 STEMI patients in our hospital were treated with fibrinolytic agents combined with or without clopidogrel.There were 84 patients in clopidogrel group,including 66(78.6%)male,18(21.4%)female and 45(53.6%)elderly patients(aged ≥ 60 years);74 patients were in control group,including 58(78.4%)male,16 female(21.6%)and 40(54.1%)elderly patients.Clinical characteristics,patency of infarct-related artery(IRA),30 d major adverse cardiac events(MACE),recurrent angina and hemorrhage events of the two groups were retrospectively analyzed.Efficacy and safety of fibrinolytic therapy combined with clopidogrel in elderly patients(≥60 years old)subgroup were also estimated.Results Baseline clinical characteristics between the two groups were comparable.Clopidogrel therapy was associated with a higher proportion of patent IRA(69.1% vs 51.4%,P=0.035)and lower rates of cardiac death(0 vs 6.8%,P=0.047)and MACE(2.4% vs 12.2%,P=0.036).There was no significant difference in the incidence of recurrent angina(3.6% vs 2.7%,P=0.885),slight and severe hemorrhage events(7.1% vs 9.5%,P=0.811;1.2% vs 1.4%,P=0.533)between the two groups.In elderly patients,clopidogrel therapy was associated with a higher proportion of patent IRA(68.9% vs 45.0%,P=0.045),the declining tendency in MACE(2.2% vs 12.5%,P=0.155),but no significant differences in the other indexes.There were no significant differences in above-mentioned indexes between the elderly and younger patients in clopidogrel group.Conclusion Addition of clopidogrel to fibrinolytic therapy is safe and effective for both the elderly and younger STEMI patients.

T was genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)in all the patients.Results The occurrence of CR in this population was 23.3%(70/300).There was CYP3A4 894C/T polymorphism in the study population.The frequencies of the three kinds of genotypes(CC,CT,TT)in CR group and non-CR(NCR)group were 45.7%,50.0%,4.3% and 63.5%,31.7%,4.8%,respectively.The frequency of TT genotype was significantly higher in NCR group than that in CR group(OR=2.06,95% CI:1.201-3.547,P=0.020).C allele carriers were more likely to develop clopidogrel resistance compared with that of T allele carriers(OR=1.59,95% CI:1.037-2.442,P=0.023).Conclusion CYP3A4 gene 894C/T polymorphism is associated with the risk of CR,and C allele carriers may be a possible genetic susceptibility factor for patients with CR.