Complications may occur inevitably during percutaneous coronary intervention(PCI),and sometimes can be life-threatening.These complications occur in approximately 5.53% of patients at large PCI centers in China.Severe complications include coronary artery perforation,acute coronary closure,no flow and acute thrombosis.Efforts should be exerted to prevent and avoid these complications during perioperative period of PCI.

Objective To investigate the change of permeability of HUVECs monolayer induced by thrombin or Lipopolysaccharide(LPS) for exploring the role of RhoA.Methods The effect of thrombin or LPS on the permeability of HUVECs monolayer was examined with the polycarbonate transwell filters and the effect of thrombin or LPS on HUVECs F-actin was observed with immunocytochemistry.The interferring action of Y-27632(Rho kinase inhibitor) on the permeability and F-actin change induced by thrombin or LPS was also observed.The expression of RhoA protein and mRNA in HUVECs were detected by Western blotting and semi-quantitative RT-PCR.Results Thrombin or LPS induced F-actin depolymerized and significant increase in the permeability of HUVECs.Permeability and F-actin do not change significantly when Y-27632 was added.Thrombin or LPS could upregulate the expression of RhoA protein and mRNA in HUVECs.Conclusion HUVECs monolayer permeability injury induced by thrombin or LPS is related to depolymerization of F-actin and RhoA signal pathway is involved.

The well performance of everything in the peripheral operative period is important to improve successful rate, decrease complications and assure favorable convalescence of patients for interventional treatment of coronary heart disease. It is mentioned that the primary management of peripheral operative period and main notes for interventional treatment of coronary heart disease in the paper summarily.

The efficacy of the standard first-line chemotherapy regimen for advanced non-small cell lung cancer (NSCLC) has reached a plateau.Study confirmed that chemotherapy drugs and molecular targeted drug maintenance treatment can prolong the survival of patients with advanced NSCLC.Immunotherapy has become a maintenance treatment for advanced NSCLC,and further research is needed to confirm its efficacy.

80 years)in China is increasing.As a special type of hypertension,the hypertension in the very elderly has some specificities in pathogenesis,clinical manifestation,treatment and prognosis.The topic to effectively cure and prevent hypertension in the very elderly is increasingly emphasiced.This article presents an overview of recent progress in clinical characteristics,and treatment strategy related to hypertension in the very elderly.

Objective An intensive analysis on the recent advances in cardiovascular medicine both in China and abroad was made,so as to provide an orientation of developing cardiovascular medicine in the future.Methods Literature of cardiovascular medicine published domestically and abroad in recent 5 years was reviewed.The advances in the use of new drug and techniques,clinical diagnosis and therapeutic methods were analyzed.Results Cardiovascular medicine in basic,clinical and practical fields had made rapid advances in the military institutions lately,especially in the interventional diagnosis and treatment of coronary heart disease,arrhythmia and peripheral vascular disease,and in the treatment of hypertension,where outstanding successes had been achieved.Conclusions The general level of cardiovascular medicine of military medical establishments could be considered foremost in our country.During the coming years of "the 12th Five-Year Plan",the emphasis of research work should be placed upon the medical care,basic and clinical research of common cardiovascular diseases existing in military officers and soldiers,such as coronary heart disease,peripheral vascular disease and arrhythmias,both in peace time and military conflicts.

Contrast induced nephropathy is an important complication arising from use of iodinated contrast media.Such iatrogenic complication may result in poor prognosis and additional health care costs.Cases of contrast induced nephropathy are on the rise along with the-increasing number of contrast-requiring procedures.Contrast induced nephropathy has been a subject of concern to cardiologists in recent years.Several large series have shown no approved methods for management of periprocedural contrast induced nephropathy.However,identification of high-risk patients,improvement and rectification of related risk factors,use of iodinated contrast media with less renal toxicity,and sufficient hydration may reduce the incidence of contrast induced nephropathy.

Objective To improve disadvantages such as asynchrony during embryoid bodies(EBs)preparation,and to establish a novel method to prepare EBs by using attached mouse embryonic stem Cells.Methods Mouse R1 embryonic stem(ES)cells were trypsinized to a single cell suspension when reaching a sub-confluent state of 70 %～80 %,then 1?106 ES cells were plated into 100 mm tissue culture dishes.After being cultured in medium containing low concentration leukemia inhibitory factor(LIF,1 ?g/L)for 3 days,EBs were collected and suspended in EBs culture medium for further development.Morphology studies were performed on suspended EBs and their cryosections,then immunofluorescent staining of ?-fetoprotein(AFP),platelet endothelial cell adhesion molecule-1(PECAM-1),and neurofilament 68 KD(NF-68)were performed on suspended and attached EBs.Results EBs obtained by this method were homogeneous in size,synchronous in developmental stage;typical in structure and could well display the developmental process from simple EBs to mature cystic EBs.Immunofluorescent staining showed that AFP,PECAM-1 and NF-68 were positive.Conclusion Compared with present methods of preparing EBs,this novel method have advantages of simple manipulation,high efficiency of EBs formation and obtaining EBs at synchronous developmental stage,furthermore,these EBs have typical structures and the potential to differentiate into derivatives of all three embryonic germ layers.Thus this method can be used as an ideal tool for studies on early embryonal development,ES cell differentiation and so on.

Objectives To evaluate the safety and effect of transradial approach for coronary intervention. Methods A total of 165 cases of coronary artery disease in this hospital accepted coronary intervention by transradial approach. Three of them had acute myocardial infarction, 112 had unstable angina, and 67 failed by transfemoral approach. Results The proportion of multivessel diseases were 60 6% and C type lesions were 20 7%. The interventons were performed in totally 294 target vessels and 299 target lesions and 246 stents were implanted. Coronary intravescular ultrasound were performed in 14 cases and cutting balloon technique in 16 cases. During intervention 1 case had right coronary spiral dissection and was successfully treated by stenting. One case had subacute thrombosis after intervention and was successfully treated by re-intervention. There were no other severe complications in this group. The mean hospital stay was (2 1?0 6) days. The total success rate was 98 2%(162/165) for transradial approach. The interventions in two cases who had chronic total occlusion in circumflex artery failed by transradial but succeeded by transfemoral thereafter .Conclusions Transradial approach for coronary intervention is a safe and effective approach so long as standardized manipulation is practised.

Objective To evaluate the safety and feasibility of transradial approach for coronary angiography.Methods Five hundred and ten cases of suspected coronary artery disease received coronary angiography by transradial approach.Results The procedures were successfully performed in 503 cases. Seven failed, of them, three failed in acupuncture, 1 had deformed radial artery,2 were with severe tortuosity of nonname artery and one case was due to left subclavian artery occlusion. The radial artery was occluded in one case after operation.There was no other severe complication. Conclusions Transradial approach for coronary angiography is safe and feasible.Its advantage lies in higher success rate and less complication.