The well performance of everything in the peripheral operative period is important to improve successful rate, decrease complications and assure favorable convalescence of patients for interventional treatment of coronary heart disease. It is mentioned that the primary management of peripheral operative period and main notes for interventional treatment of coronary heart disease in the paper summarily.

Objective To investigate the change of permeability of HUVECs monolayer induced by thrombin or Lipopolysaccharide(LPS) for exploring the role of RhoA.Methods The effect of thrombin or LPS on the permeability of HUVECs monolayer was examined with the polycarbonate transwell filters and the effect of thrombin or LPS on HUVECs F-actin was observed with immunocytochemistry.The interferring action of Y-27632(Rho kinase inhibitor) on the permeability and F-actin change induced by thrombin or LPS was also observed.The expression of RhoA protein and mRNA in HUVECs were detected by Western blotting and semi-quantitative RT-PCR.Results Thrombin or LPS induced F-actin depolymerized and significant increase in the permeability of HUVECs.Permeability and F-actin do not change significantly when Y-27632 was added.Thrombin or LPS could upregulate the expression of RhoA protein and mRNA in HUVECs.Conclusion HUVECs monolayer permeability injury induced by thrombin or LPS is related to depolymerization of F-actin and RhoA signal pathway is involved.

Complications may occur inevitably during percutaneous coronary intervention(PCI),and sometimes can be life-threatening.These complications occur in approximately 5.53% of patients at large PCI centers in China.Severe complications include coronary artery perforation,acute coronary closure,no flow and acute thrombosis.Efforts should be exerted to prevent and avoid these complications during perioperative period of PCI.

The efficacy of the standard first-line chemotherapy regimen for advanced non-small cell lung cancer (NSCLC) has reached a plateau.Study confirmed that chemotherapy drugs and molecular targeted drug maintenance treatment can prolong the survival of patients with advanced NSCLC.Immunotherapy has become a maintenance treatment for advanced NSCLC,and further research is needed to confirm its efficacy.

80 years)in China is increasing.As a special type of hypertension,the hypertension in the very elderly has some specificities in pathogenesis,clinical manifestation,treatment and prognosis.The topic to effectively cure and prevent hypertension in the very elderly is increasingly emphasiced.This article presents an overview of recent progress in clinical characteristics,and treatment strategy related to hypertension in the very elderly.

Objective An intensive analysis on the recent advances in cardiovascular medicine both in China and abroad was made,so as to provide an orientation of developing cardiovascular medicine in the future.Methods Literature of cardiovascular medicine published domestically and abroad in recent 5 years was reviewed.The advances in the use of new drug and techniques,clinical diagnosis and therapeutic methods were analyzed.Results Cardiovascular medicine in basic,clinical and practical fields had made rapid advances in the military institutions lately,especially in the interventional diagnosis and treatment of coronary heart disease,arrhythmia and peripheral vascular disease,and in the treatment of hypertension,where outstanding successes had been achieved.Conclusions The general level of cardiovascular medicine of military medical establishments could be considered foremost in our country.During the coming years of "the 12th Five-Year Plan",the emphasis of research work should be placed upon the medical care,basic and clinical research of common cardiovascular diseases existing in military officers and soldiers,such as coronary heart disease,peripheral vascular disease and arrhythmias,both in peace time and military conflicts.

Contrast induced nephropathy is an important complication arising from use of iodinated contrast media.Such iatrogenic complication may result in poor prognosis and additional health care costs.Cases of contrast induced nephropathy are on the rise along with the-increasing number of contrast-requiring procedures.Contrast induced nephropathy has been a subject of concern to cardiologists in recent years.Several large series have shown no approved methods for management of periprocedural contrast induced nephropathy.However,identification of high-risk patients,improvement and rectification of related risk factors,use of iodinated contrast media with less renal toxicity,and sufficient hydration may reduce the incidence of contrast induced nephropathy.

Objective To explore possible role of cellular repressor of E1A-stimulated genes(CREG) in the process of phenotypic switching of adventitial fibroblasts(AFs). Methods Immunofluorescent staining was performed with tissue sections from mouse carotid arteries to evaluate the relationship between the expression of CREG and smooth muscle actin-α(α-SMA) in injured arteries, especially in the adventitia. Tissue block pasted culture method was used to isolate and culture AFs. RT-PCR and Western-blot were used to detect the change of CREG andα-SMA mRNA and protein expression in AFs in the presence of different concentrations of AngⅡfor 12 h/24 h or in the presence of 100 nmol/L Ang Ⅱ for different times. Results Normal mouse carotid arteries had little α-SMA expression throughout the tunica adventitia. Arteries at day 1 and day 3 post-injury exhibited significantly higher immunofluorescence of α-SMA compared with non-injured arteries. Alpha-SMA expression began to decrease on day 7 and progressively declined on day 14. In contrast, immunofluorescent staining revealed that CREG was expressed in the adventitia of normal arteries. Expression of CREG in the adventitia of injured arteries was decreased on the 1st day, reached its lowest value on the 3rd day, and increased gradually from the 7th day, and was higher compared with that in non-injured arteries on the 14th day after injury. Similarly, the expression of CREG in AFs was very high, and AngⅡremarkably decreased mRNA and protein expression levels of CREG in a dose-dependent and time-dependent manner. Conclusions The changes in CREG expression correlate with AF phenotypic modulation, and CREG down-regulation may facilitate AF phenotypic switching into myofibroblasts (MFs).

Objective To establish a method for determination of 7 preservatives, benzyl alechol, sorbic acid, benzoic acid, methyl-paraben, ethyl-paraben, propyl-paraben, butyl-paraben, in cosmetics in one time by gas chromatography. Methods The cosmetic sample was dissolved by methanol, extracted by ultrasonic and separated by packed column of DB1, the optimized experimental condition was explored also. Results The concentrations of 7 preservatives showed a positive correlation with the peak heights at the following ranges, 100-500 ?g/ml for benzyl alechol, 200-600 ?g/ml for sorbic acid, 25-500 ?g/ml for benzoic acid, methyl-paraben, ethyl-paraben, propyl-paraben and butyl-paraben, the correlative coefficients were 0.998 3-0.999 9. The detection limits were 5.5, 57.1, 29.6, 6.2, 5.5, 6.2, 6.2 ng/ml respectively. The recovery rates were 91.6%-102.7%. RSDs were 2.2%-10.2%. Conclusion The present method can perfectly separate the seven preservatives in cosmetics mentioned above with some advantages, such as simple, accurate, sensitive and quick.

Objective To evaluate mid-term clinical result of drug-eluting stent (DES) implantation for patients with multivessel chronic total coronary occlusion (CTO) lesions. Methods We analyzed 89 patients who underwent interventional revascularization in their multivessel CTO lesions with DES from November 2002 to June 2005 (DES group). A control group was composed of 70 consecutive patients with multivessel CTO lesions treated with bare metal stents (BMS) from November 2000 to June 2003 (BMS group). Results There were no significant differences in basic clinical manifestations and success rate of intervention procedures between DES and BMS group. At 6-month follow-up, the cumulative rate of major adverse cardiac events (MACE) was 14.6% in the DES group and 28.6% in the BMS group (P=0.031). The incidence of restenosis was 16.9% in the DES group and 34.3% in the BMS group (P=0.011). The need for target vessel revascularization and target lesion revascularization in the DES group was significantly lower (9.0% vs 21.4%, P=0.003, and 7.9% vs 18.6%, P=0.043, respectively) compared with BMS group. Conclusion It is possible to obtain an ideal mid-term clinical outcome in patients with multivessel CTO lesions by DES implantation.