The effects of different methods of psychological treatment in psychological rehabilitation of breast cancer patients were reviewed. Group psychotherapy is the best choice of breast cancer patients for psychological rehabilitation.

Objective To explore the relationships among mobile phone dependence and attachment,social support among adolescent students.Methods 900 students from two vocational colleges in Shandong province were recruited by stratified sampling.They were assessed with Experiences in Close Relationships Inventory (ECR) Chinese version,Social Support Rating Scale and Mobile Phone Addiction Tendency Questionnaire.Results There were no significant differences of adolescent students'mobile phone dependence on gender,child status,and native place.The score of middle vocational school students'mobile phone dependence (45.84 ± 12.64)was significantly higher than that of higher vocational college students (40.68 ± 9.72),and there was significant difference between them (P ＜ 0.01).Mobile dependence was significantly positively related with attachment anxiety (r =0.469,P ＜ 0.01),and was significantly negatively related with social support (r =0.149,P ＜ 0.01).Attachment anxiety and social support were the direct predictors of mobile phone dependence,and social support played the partial mediating role between attachment anxiety and mobile phone dependence.Conclusions Mobile phone dependence is a common phenomenon among adolescent students,and the middle vocational school students' mobile phone dependence is more serious than that of the higher vocational college students.There are close relationships between mobile phone and attachment,social support.Attachment anxiety is not only directly but also indirectly associated with mobile phone dependence by social support.

Objective To evaluate effects of different laparoscopic procedures for hydrosalpinx on ovarian responses to superovulation induction during in vitro fertilization-embryo transfer(IVF-ET).Methods A total of 65 patients with infertility derived from hydrosalpinx underwent 67 cycles of IVF-ET from January 2002 to January 2005.Before the IVF-ET,the patients were given bilateral laparosocpic salpingectomy(Group A,n=47,48 cycles) or laparosocpic proximal tubal ligation and distal tubal salpingostomy(Group B,n=18,19 cycles).Another 58 patients with proximal tubal occlusion without hydrosalpinx were simultaneously included for 58 cycles of IVF-ET(Group C).Results The fertilization rates,cleavage rates,implantation rates,and clinical pregnancy rates were 85%?21%,95%?17%,49%(55/113),and 79%(38/48) in the Group A,83%?17%,98%?7%,47%(21/45),and 68%(13/19) in the Group B,and 81%?22%,92%?16%,41%(57/140),and 66%(38/58) in the Group C,respectively,without statistically significant difference among the 3 groups(F=0.48,P=0.620;F=1.22,(P=0.297);?~2=1.691,P=0.429;?~2=2.471,P=0.291).The doses of gonadotrophin,the duration of stimulation,and the number of retrieved oocytes were 29.4?10.3 vials,9.4?1.7 d,and 11.4?5.6 in the Group A,24.7?7.7 vials,9.0?1.8 d,and 12.8?6.1 in the Group B,and 28.4?8.8 vials,9.1?1.8 d,and 11.4?5.3 in the Group C,respectively,without statistically significant difference among the 3 groups(F=1.77,P=0.174;F=0.52,P=0.593;F=0.51,P=0.599).Conclusions Laparosocpic salpingectomy or laparosocpic proximal tubal ligation with distal tubal salpingostomy for hydrosalpinx does not impair ovarian responses to the superovulation induction.Both of the procedures may be considered for the treatment of hydrosalpinx prior to IVF-ET.

Objective To study the embryotoxicity and teratogenicity of monocrotophos in mice.Methods Sixty pregnant Kunming mice were randomly divided into four groups,15 in each:three groups were exposed to the monocrotophos at the doses of 0.05,0.10 and 0.20 mg/kg,10 ml/kg through gavage,during the period of organ formation(from 7th day to 16th day),once a day and the control group to distilled water.The pregnant mice were weighted in day of 0,6,12,18,and 20,and were sacrificed on 20th day of pregnancy.The numbers of living,dead and absorbed fetus were counted,and the uterus and placenta were weighted.The deformity examination was conducted.Results The maternal body weight of exposure groups were lower than those of control group in 12,18 and 20 day of pregnancy.With the increased doses of monocrotophos,the weights of pregnant mice showed a downward trend.Compared with the control group,the rates of dead fetus and absorbed fetus increased significantly,and the rates of living fetus,the fetal body weight and body length,tail length and placental weight decreased significantly(P

Objective To explore the effects of group psychotherapy on breast cancer patients. Methods 80 outpatients with breast cancerwho did not participate in the Rukang Salon (control group) and 100 outpatients participated (intervention group) were surveyed withquestionaire. Results The incidence of psychological distress was 36.8% in the control group and 21.1% in the intervention group ( χ2=5.02,P=0.03). In the intervention group, the efficacy of those without psychological distress (236.86±35.17) was significantly higher than thosewith psychological distress (214.21±26.52), (t=2.61, P=0.01). There were significant differences in those 5 factors: group cohesion, universality,interpersonal learning, guide instructions, and hope remodeling. Top 5 factors in scores reported were interpersonal learning, hopingremodeling, group cohesion, guide instructions, and exist consciousness. Conclusion Group psychotherapy can significantly reduce the psychologicaldistress in rehabilitating breast cancer patients. Group cohesion, universality, interpersonal learning, guide instructions, hope remodelingand exist consciousness may play important roles.

Objective To investigate the life satisfaction and its related factors in primary caregivers of breast cancer patients. Methods The Life Satisfactory Index A (LSIA) and self-administrated questionnaire were used. The life satisfaction between breast cancer patients'primary caregivers and breast benign patients' primary caregivers were compared. The factors of the life satisfaction in primary caregivers of breast cancer patients were analyzed. Results The life satisfaction was poorer in the breast cancer patients' primary caregivers than in the breast benign patients' primary caregivers (P<0.01). The life satisfaction in the breast cancer patients' primary caregivers was correlated with the stage of breast cancer, course of disease, primary caregivers' age and family monthly income (P<0.05). Conclusion The life satisfaction in breast cancer patients' primary caregivers is worse and is related with stage of breast cancer, course of disease, primary caregivers' age and family monthly income.

Objective To explore the relatianship of social anxiety and parental rearing style and self-es-teem among medical students, and to provide theoretical guidance for students' mental health. Methods 346 medical students were assessed with interaction anxiousness scale(IAS), Egala minnen on bardndosnauppforstran (EMBU) and self-esteem scale (SES). Results Medical students' social anxiety was negatively correlated with emotional warmth and understanding of parents (FF1, MFI) (r=-0.162,-0.150, P<0.01) and self-esteem (r -0.355, P <0.01), and positively correlated with mother's over-interference, over-protection, refusal, rejection and favoring subject(MF2, MF3, MF5) (r=0.137,0.127,0.116, P<0.05). Stepwise regression analysis re-vealed that father's warmth and understanding and father's over-protection and self-esteem enter regression equa-tion and adjusted R square was 0.047 and 0.123. Conclusion The medical students' social anxiety was influ-enced by EMBU and SES, but the level was lower.

Objective To explore the genetic prenatal diagnosis method for acbendroplasia (ACH).Methods During May to November 2007, three ACH pedigrees were diagnosed at the Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, Affiliated Drum Tower Hospital of Medical College, Nanjing University. In family 1, there was a 6-month-old male ACH infant. In family 2, the expectant mother, with 18 weeks of pregnancy, was an ACH patient. Amniocentesis was performed for prenatal diagnosis. The fetus of family 3 was diagnosed as ACH by ultrasound examination on the 39th week of gestation. Umbilical cord blood of this fetus was collected for examination. Totally, three methods, restriction enzyme (Sfc Ⅰ and Msp Ⅰ ) digestion analysis, denaturing high performance liquid chromatography (DHPLC) and sequencing analysis were performed simultaneously to detect the pathogenic mutation of flbroblastic growth factor receptor 3 (FGFR3) for the three ACH families. Results ( 1 ) The DHPLC detection: heteroduplex was detected in the patient of family 1 ; beth the patient and the fetus of family 2 showed heteroduplex results; the result of the fetus of family 3 was also heteroduplea. (2) The enzyme digestion analysis for the PCR products of 10 exon of FGFR3: after Sfc Ⅰ digestion, the PCR products of patients and the fetus of family 1 and 2 showed not only the band of 247 bp, but also bands of 162 bp and 85 bp. But their PCR products could not be digested by Msp Ⅰ , and it only showed the band of 247 bp. For the fetus of family 3, the PCR products could not be digested by Sfc Ⅰ , while after digestion by Msp Ⅰ , bands of 162 bp and 85 bp were shown up. The PCR products of the normal control could be digested by neither Sfc Ⅰ nor Msp Ⅰ. (3) The sequencing results: the heterozygote mutation of 1138 C→A was confirmed in the patient of family 1. The pregnant woman and her fetus in family 2 showed the same result. The heterozygote mutation of C→C was confirmed in the fetus of family 3. The site of 1138 was G homozygote in the normal control The three detection results of the fetus in family 2 were the same as that of the mother, which means that the fetus inherited the same pathogenic mutation from his or her mother. Conclusions Both DHPLC and restriction enzyme digestion analysis could detect the mutation of FGFR3 gene, but DHPLC is more rapid, convenient and sensitive. So DHPLC can be applied to genetic diagnosis and prenatal diagnosis for ACH patients.

Objective To study effect and its possible mechanism of total glucosides of paeony ( TGP ) on adriamycin-induced nephropathy rats.Methods Thirty male SD rats were randomly divided into normal group ( CON) , adriamycin-induced nephropathy group ( ADRN) and TGP-treated ADRN group (TGP).The rat nephropathy model was established by adriamycin injection.At the end of the 8th week after treatment, ELISA was used to detect the level of Cr and BUN.The values of 24 urine protein was determined by urinary protein kit.Masson staining was used to observe the fibrosis.RT-PCR was used to detect the mRNA levels of TLR4/NF-κB/TGF-β1 .Immunohistochemical method was used to detect the content of TLR4/NF-κB/TGF-β1.ResuIts Compared with the CON group, the level of Cr, BUN, 24 urine protein of ADRN group rised(P<0.01), the degree of fibrosis of ADRN group were aggravated(P<0.01), and the expressions of TLR4/NF-κB/TGF-β1 of ADRN group increased significantly.However, compared with ADRN group, the(P<0.01) level of Cr, BUN, 24 urine protein of TGP-treated rats reduced(P<0.01), the degree of fibrosis of TGP-treated rats eased(P<0.05), and the expressions of TLR4/NF-κB/TGF-β1 of TGP-treated rats decreased significantly.ConcIusion TGP retards the process of fibrosis and reduces the pathological damage in adriamycin-induced rats by down-regulating the expression of TLR4/NF-κB/TGF-β1 signaling.

ObjectiveTo investigate the relationship between cytokine gene polymorphisms and long-term humoral response in children vaccinated against hepatitis B during infancy.MethodsA total of 293 children (6.08±0.59 years old) who received three doses of hepatitis B vaccine according to a 0-,1-,6-month schedule during infancy and were negative for HBsAg and/or anti-HBc,were enrolled.Of them,83children with anti-HBs ＜10 mIU/ml were considered as long-term poor responders ( group A),and 210 others with anti-HBs ≥ 10 mIU/ml were defined as long-term responders ( group B).A total of 11 single nucleotide polymorphisms (SNPs) of IL-1β,IL-2,IL-4,IL-10,IL-12B,and IL-13 were detected with PCR-restriction fragment length polymorphism.ResultsThe allele frequencies of -33T,-589T,and 2979T in IL-4 gene in group A were 86.1%,86.1% and 90.4%,respectively,higher than those in group B (76.0%,76.9%,and 83.3%,respectively,all P＜0.05).In IL-4 gene,frequencies of TT genotype at position -33 and -589 in group A were 74.7% and 75.9% respectively,higher than those in group B (57.1% and 59.0% respectively,both P＜0.01 ),while the frequencies of CT at position -33 and -589 in group A was 22.9% and 20.5% respectively,lower than those in group B (37.6% and 35.7% respectively,both P＜0.05).The genotype distributions of IL-4 2979 and the allele or genotype distributions of 8 other SNPs showed no significant differences between group A and group B.ConclusionPolymorphisms at position -33,-589,and 2979 in IL-4 gene are associated with the long-term humoral response in children vaccinated against hepatitis B during infancy.