Stricture and insufficiency of eustachian tube in 43 cases were treated by floating duct for dilation of eustachian tube under fiber endoscopy. Clinical observation lasbed 2-10 months. 23 cases were significantly effective, 13 cases effective. No intraoperative complications.

Objective To study the embryotoxicity and teratogenicity of monocrotophos in mice.Methods Sixty pregnant Kunming mice were randomly divided into four groups,15 in each:three groups were exposed to the monocrotophos at the doses of 0.05,0.10 and 0.20 mg/kg,10 ml/kg through gavage,during the period of organ formation(from 7th day to 16th day),once a day and the control group to distilled water.The pregnant mice were weighted in day of 0,6,12,18,and 20,and were sacrificed on 20th day of pregnancy.The numbers of living,dead and absorbed fetus were counted,and the uterus and placenta were weighted.The deformity examination was conducted.Results The maternal body weight of exposure groups were lower than those of control group in 12,18 and 20 day of pregnancy.With the increased doses of monocrotophos,the weights of pregnant mice showed a downward trend.Compared with the control group,the rates of dead fetus and absorbed fetus increased significantly,and the rates of living fetus,the fetal body weight and body length,tail length and placental weight decreased significantly(P

0.05). Conclusions The presentation of activated NK and T cells are both involved in the mechanism of immune tolerance of pregnancy, and the immunity of NK and T cells in basal decidua is independent of the systematic immunity in peripheral blood.

Objective To evaluate status of female pelvic floor function and sexual life after total hysterectomy.Methods From March 2001 to January 2004,92 patients with undergoing hysterectomy due to benign gynecological diseases were enrolled in this study.They were followed up at outpatient department,including pelvic examination,filling in female sexual quality questionnaire,pelvic floor distress inventory short form 20 ( PFDI-20 ),pelvic floor distress impact questionnaire short form 7 ( PFIQ-7 ) and quality of sexual life of chinese women questionnaire.Results At 6 years after total hysterectomy,it was observed that 7 cases (7/92,7.6％ ) were pelvic organ prolapse and 62 cases (62/92,67.4％ ) were urinary incontinence.A median score of PFDI-20 were 4.67.A median score of PFIQ-7 were 0.Symptoms of pelvic floor dysfunction concentrated in lower urinary tract ( 58 cases with cough leak,32 cases with spot urine leakage,31 cases with frequent micturition,24 cases with urgent urination)and bowel symptoms (26 cases with constipation,24 cases with defecation urgency,21 cases without fully drained stool).In the 68 patients filling in female sexual quality questionnaire,an average score were (127 ± 20) points.Female sexual quality questionnaire score,sexual satisfaction,sexual communication and adjustment,sexual response and sexual body image were positively correlated with the patients' income ( r = 0.432,P = 0.007 ; r = 0.356,P =0.028;r=0.475,P=0.003;r=0.421,P=0.009;r=0.324,P=0.047).Conclusions Hysterectomy may have long-term effect on female pelvic floor function and sexual life.Quality of sexual life in those patients was positively correlated with income.

In vitro diagnostic ( IVD) manufacturers were important suppliers of external servicesto medical laboratories .There equipment , reagents , calibration and maintenauce services played an important role in ensuring the quality of medical laboratory tests .It is also helpful for IVD manufacturers to establish and maintain a good reputation and credibility .ISO 15189:2012“Medical laboratories -Requirements for quality and competence” was issued onNovember 1, 2012, which replaced the second edition (ISO 15189:2007).Requirements related to IVD manufacturers are involved in the new edition standard .The article aimed to help medical laboratories and IVD manufacturers understand the requirements ; therefore , laboratories would know how to select suppliers based on their ability to supply external services , IVD manufacturers would know how to meet the needs of laboratories , and the cooperation between IVD manufacturers and laboratories would be facilitated .

Objective　To investigate exogenous ceramide-induced apoptosis in colon carcinoma LoVo cells.Methods　LoVo cells were pretreated for 3 h with or without the presence of phorbol 12-myristate 13-acetate (PMA), a potent protein kinase C (PKC) stimulator, later treated with C2-, C6-ceramide or C2-dihydroceramide, and then passed through gel electrophoresis, Heochest 33342 fluorescence staining and flow cytometry with Annexin V/PI staining. The treated LoVo cells were observed for biochemical and morphologic changes. Results　Treatment with C2- or C6-ceramide in the range of indicated concentrations (10～25 μmol/L) for 12 to 24 hours resulted in apoptosis in LoVo cells, whereas C2-dihydroceramide, which is similar to C2-ceramide in configuration but lacks the trans double bond at C4-C5 of the sphingoid base backbone, did not induce the apoptosis at the same or even higher concentrations, indicating that the ceramide-induced apoptosis was stereospecific. Moreover, the exogenous ceramide-induced apoptosis in LoVo cells was inhibited in part by PMA. Conclusion　Ceramide takes part in the process of apoptotic signal transduction in LoVo cells. PKC may be one of downstream target molecules acted by ceramide.

Objective To observe the expressions of Akt and ERK phosphorylation in abdominal aortic aneurysm of rat model, and explore the pathogenesis of abdominal aortic aneurysm. Methods The rat model of abdominal aortic aneu-rysm was established. The diameter of abdominal aorta was measured and the extended rate of the aorta was calculated. HE staining was used to observe the change of pathology. Immunohistochemistry and Western blot methods were used to detect the expressions of Akt and ERK phosphorylation in the level of protein. Results The dilation of aorta was significantly high-er in abdominal aortic aneurysm group than that of saline group and normal group (P＜0.05). HE staining showed structural disorder and inflammatory cell infiltration in abdominal aortic aneurysm group. The results of immunohistochemistry and Western blot results showed that phosphorylation of Akt expression was significantly higher in abdominal aortic aneurysm group than that of saline group and normal group (P＜0.05). There was no significant difference in phosphorylation of ERK expression between three groups (P>0.05). Conclusion PI3K/Akt signaling pathway may be involved in the development of abdominal aortic aneurysm.

Objective To study the effects of enteral nutrition( EN) on inflammatory factors and cardiac function in elderly patients with heart failure ( HF ) . Methods A total of 96 elderly patients with HF were enrolled in this study and randomly divided into treatment group A,treatment group B and control group( n= 32 in each group) . Patients in group A were provided with 500 mL of EN daily for 1 month, and those in group B were provided with the same dose of EN for 3 months. Control group was treated with normal diet. The nutrition status of each group was assessed by NRS2002. Meanwhile,all patients were evaluated by New York Heart Association(NYHA) class and left ventricle eject fraction(LVEF). The levels of BNP,IL-6, CRP and TNF-α were also measured before and after nutrition treatment. Results In group A and group B,BMI,TSF,AMC, TP,ALB and HGB increased. Symptoms of HF alleviated quickly and LVEF increased compared to the baseline. The levels of BNP,IL-6,CRP and TNF-α were significantly lower after treatment(P<0. 05) in both treatment groups,and the change in group B was more obvious(P<0. 01) than group A. However,in control group,there is neither evident improvement in cardiac function or nutrition status,nor in inflammatory factor levels. Conclusion Adding EN support to the normal treatment of heart failure in elderly malnourished patients with HF not only improves the function of heart and nutrition status, but also helps to support immune system so as to alleviate acute inflammatory response. The benefits from EN depend on the duration of therapy.

Objective To describe X-ray and CT findings of lung in paraquat poisoning.Methods CT and X-ray data of chest in 21 patients with paraquat poisoning were retrospectively analyzed.Results X-ray and CT findings of lung were varied with different time.≤7 d,there were mainly increase of lung marking in 17 cases,ground-grass attenuation in 14 cases and consolidation in 2 cases.7~14 d,increase of lung marking,consolidation and pulmonary fibrosis each could be seen in 4 cases,and 3 cases with ground-grass attenuation.≥14 d,there were pulmonary fibrosis in 4 cases,bronchiectasis in 3 cases,increase of lung marking in 2 cases.Conclusion Chest plain X-ray film and CT are of important value in observing the evolution,evaluating the prognosis and guiding the treatment project for the patients with paraquat poisoning.

AIM: To deplore the adjustion of whole peptidoglycan of bifidobacterium bifidum to functions of macrophages.METHODS:Levels of interleukin 6, interleukin 12 produced by peritoneal macrophages of nude mice was detected by ELISA method, and the content of nitric oxide (NO) was detected by using Griess reagent, after whole peptidoglycan were injected into nude mice peritoneally. RESULTS:The content of interleukin 6, interleukin 12 and NO secreted from peritoneal macrophages of nude mice in the whole peptidoglycan injection group was 732.54?190.30(pg/mL)、816.37?96.40(pg/mL) and 48.90?6.51(?mol/L) respectively.It was 303.78?171.75(pg/mL), 510.27?123.46(pg/mL) and 30.67?12.83(?mol/L) respectively in the control group. In view of the content of interleukin 6, interleukin 12 and NO, there existed significant difference when whole peptidoglycan injection group was compared with control group( P