Objective To study and research the clinical pathologic features, immunohistochemistry and prognosis of epidermoid cyst with cancerous changes. Methods Six cases of epidermoid cyst with cancerous changes were studied of their clinical pathologic features and immunohistochemistic expression and their prognosis was evaluated by follow-up vis-its. Results Clinical features: All six cases showed local enclosed mass and underwent extensive resection. No recur-rence nor metastasis were shown in one year follow up. Pathological examination show irregular cystic mass with bean curd like fillings in it. Microscopic examination showed those cysts were lined with squamous epithelium which indicate invasive growth. Pathological diagnosis: Epidermoid cyst canceration to squamous cell carcinoma. Immuno-phenotype: CK 5/6 and P63 were positive in all six squamous cell cancer cases. Conclusion Epidermoid cyst with cancerous changes are quite rare. Diagnosis of the tumor largely relies on histopathology and immunohistochemical markers also assist diagnosis. Local appropriate expanded resection is preferred. Its malignant degree is low and prognosis is good.

Objective:To investigate clinical and dermoscopic manifestations of nail damage secondary to hand, foot and mouth disease.Methods:Clinical data were collected from 12 patients with nail damage secondary to hand, foot and mouth disease in Department of Dermatology, Shandong Provincial Hospital from June to November 2015, and characteristics of nail damage were analyzed.Results:The clinical manifestations of nail damage following hand, foot and mouth disease included dryness of periungual skin (12 cases) , nail plate cavities (11 cases) , nail fractures (11cases) and Beau′s lines (4 cases) . Dermoscopy showed nail discoloration (12 cases) , nail delamination (12 cases) , horizontal streaks (10 cases) , longitudinal streaks (8 cases) , brown background of the nail matrix (11 cases) , telangiectasia (7 cases) , periungual desquamation (12 cases) and red background (10 cases) .Conclusion:The clinical manifestations of nail damage following hand, foot and mouth disease are mainly dryness of periungual skin, nail plate cavities and fractures, and its dermoscopic manifestations include nail discoloration, delamination, horizontal and longitudinal streaks, brown background of the nail matrix, telangiectasia, periungual desquamation and red background.

Objective To inustigate oceular blood flow velocity in the eyes of paicients with diabetic retino pathy.Methods Hemodynamic parameters in the ophthalmic arteny(OA),central retinal artery(CRA) were determined with color Doppler sonography(CDS) in 70 eyes of 35 patients with diabetic retinopathy and 80 eyes of 40 patients of mormal controlts.Results Compared with normal subjects,the patients with diabetic retinopathy showed significant slower hemodynamic pararneters with OA.CRA and higher with resistive index(P

BACKGROUND:Currently, little is reported regarding the effectiveness of mineralized gelatin electrostatic spinning fibers on periodontal tissue osteogenic induction. OBJECTIVE: To investigate the effect of mineralized gelatin electrostatic spinning on the proliferation and osteogenic differentiation of periodontal ligament fibroblasts. METHODS: Human periodontal ligament fibroblasts were respectively compositely cultured with non-mineralized gelatin electrostatic spinning, gelatin electrostatic spinning after 1 day of nano-hydroxyapatite mineralization and gelatin electrostatic spinning after 5 days of nano-hydroxyapatite mineralization. Cel proliferation was determined at 1, 4, 7, 10 and 13 days of culture using MTT assay. Alkaline phosphatase activity of cels was determined at 1, 7, 14 days of culture using biochemical analyzer. RESULTS AND CONCLUSION:At the 10th day of culture, the periodontal ligament cels on the surface of membrane distributed uniformly, grew wel, spread out as sheets and secreted a large amount of extracelular matrix, linked closely with the materialsl; moreover, the effect was more obvious at 5 days of mineralization. Cel growth density and status were better than those in the non-mineralized gelatin electrostatic spinning membrane group. Cel proliferation and alkaline phosphatase activities at different time points: 5-day mineralized gelatin electrostatic spinning membrane group > 1-day mineralized gelatin electrostatic spinning membrane group > non-mineralized gelatin electrostatic spinning fibers group (alP < 0.05). These results demonstrate that the gelatin electrostatic spinning after nano-hydroxyapatite mineralization may promote the proliferation and osteogenic differentiation of periodontal ligament fibroblasts in a time-dependent manner.

Objective To study the methods of preparing samples, analyse free radical in smoke of cigarette and the relationship between free radical and the other harmful components in the cigarette smoke. Methods The contents of two types of free radical in smoke of 13 kinds of cigarettes were determined with electron spim resonance(ESR) and the self-made equipment. Results The analytic results showed that the main component was the free radical in gas state in all cigarette samples and it was about 10-30 times higher than the content of free radical in solid state. The content of free radical in gas state of cigarette made in China was lower than that made in foreign country. Conclusion The analysis method using ESR and the self-made equipment will be easily used in the related experiment; The level of free radical in gas and solid state is positively related to the level of tar and nicotine in the cigarette sample of same trademark but it is not the same in the samples of different trademark. Therefore, we can not to infer the content of free radial in cigarette smoke by the content of tar and nicotine in it,especially for the content of free radial in gas state. Using charcoal filter is benefit to reduce the level of free radical in cigarette smoke.

Objective To study the efficiency of the compound active carbon filter in reducing the content of free radicals in the smoke of cigarettes. Methods 9 trademarks of cigarettes installed the compound active carbon filters were collected and ESR was used to analyze the content of free radicals in the particle and gas phases. Results The results showed that the efficiency of the compound active carbon filters to reduce the content of free radicals in the cigarette smoke was not identical,some samples even showed an inverse result. Conclusion Based on the results of the present paper,it is not considered that the compound active carbon filter can always reduce the free radicals in the smoke of cigarettes.

Objective To study the effect of fluoride varnish on microconsistency of enamel after under irradiation in vitro. Methods Normal enamel samples were randomly divided into four groups: control group,irradiation group,fluoride varnish group and fluoride varnish-irradiation group.The microconsistency of enamel surface before and after treatment,after anti-caries test was measured by type MH-5 durometer. Results Fluoride varnish had an inhibitory effect on the decrease of enamel microconsistency etched with lactate buffer.The microconsistency in irradiation group was obviously lower than that in control group(P

Office blood pressure has been regarded as a gold standard of blood pressure measurements.It is an important indicator of ensurement of correct diagnosis,management of condition and evaluation of prognosis.But limitations of office blood pressure have led to the increasing use of out-of-office blood pressure.Many evidence show that out-of-office blood pressure could more accurately reflex variability of blood pressure and rhythm of blood pressure.Out-of-office blood pressure could diagnose white-coat hypertensives or masked hypertensives without delay,properly determine the need for antihypertensive treatment and decrease risk of developing blood pressure-related complications.

Objective To study the clinical therapeutic effect of Yifumin Capsule in treating type 2 diabetic insulin resistance and its effect on relevance indexes.Methods Two hundred and forty cases of type 2 diabetic insulin resistance with deficiency of qi-yin and stagnation of phlegm and toxin were divided randomly into two groups,120 cases in each group.The treatment group was given orally Yifumin Capsule,and the control group was given orally pioglitazone.The treatment course of the two groups was 12 weeks.Clinical symptoms,blood glucose,blood lipid,CRP,ISI,FINS,HOMA-IR,liver and kidney function were detected respectively.Results Chinese medicine symptoms and signs were all improved.The total effective rate of clinical effect was 88.1% in treatment group and 52.9% in control group.The clinical therapeutic effect of the treatment group was obviously better than the control group.Both groups had a notable seducement in HOMA-IR,FINS,FPG,2 h PG,HbA1c,TG and LDL-C with an obviously improvement in ISI after the treatment.Compared with the control group,the treatment group had a significant decrease in TC and CRP with notable difference.Conclusion Yifumin Capsule can effectively improve the insulin resistance of type 2 diabetic patients.

This essay investigated the in vitro tumoricidal activity of Con A primed murine LAK cells (Con A-LAK), discussed the role of mTNF-? in the killing effect. The results showed that Con A-LAK cells could kill NK sensitive YAC-1, TNF sensitive L929 and H-2 low/non expression HCa-F target cells; The cytotoxicitity of Con A-LAK cells was performed by direct contact, the non-secretary pathway mediated by interaction of surface ligands of effector cells and receptors of target cells play an important role; Con A-LAK cells expressed mTNF-?, which participated in the killing of TNF sensitive tumor cells and might be one of the important effector molecules in MHC non-restricted, antigen non-specific cvtotoxicitv.