Objective To evaluate the effects of fasudil on neointima hyperplasia in rat carotid artery balloon-injury model,and explore the potential molecular mechanisms of this effect.Methods Totally 30 male Sprague-Dawley (SD) rats were randomly divided into sham operation group,balloon injury group,2.5 ml/kg fasudil intervention group,5 ml/kg fasudil intervention group,10 ml/kg fasudil intervention group.The intima-to media(I/M) area ratio of neointima was calculated at 14th day after operation; the protein expression of proliferating cell nuclear antigen(PCNA) was measured by immunohistochemical and the integrated optical density (IA) was calculated.As the marker of oxidative stress,the levels of 8-hydroxy-2'-dcoxyguanosine (8OHdG) and malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELISA).The expressions of phosphorylated and non phosphorylated Ras,mitogen activated protein kinase kinase (MEK1/2) and extracellular signal regulated kinase (ERK1/2) were measured by Western blot.Results The I/M ratios were (0.22 ± 0.01) in sham operation group,(2.71 ± 0.23) in balloon injury group,( 1.72 ±0.24)in 2.5 ml/kg fasudil intervention group,(1.23±0.11)in 5 ml/kg fasudil intervention group,(0.85±0.12)in 10 ml/kg fasudil intervention group.As compared with sham operation group,I/M area ratio was increased in balloon injury group,and three fasudil intervention group inhibited neointima hyperplasia in a dose-dependent manner (all P＜0.01).The IA indexes were(15.11 ±1.42),(53.42±2.30),(45.25±1.62),(22.55±0.88) and(19.61±0.79) in the above five groups,respectively,showing the fasudil inhibited PCNA expression (all P＜0.01).Fasudil also inhibited the levels of 8-OHdG and MDA in a dose-dependent manner [8OHdG:(54.34±4.50)μg/kg,(106.81 ± 6.67)μg/kg,(94.21 ± 3.34) μg/kg,(65.58 ± 5.13) μg/kg,(51.32 ± 2.34) μg/kg; MDA:(19.94 ± 0.65 ) μmol/kg,( 58.83 ± 2.81 ) μmol/kg,(48.94 ± 1.35 ) μmol/kg,(40.62 ± 3.24 )μmol/kg,( 36.85 ± 0.91)μmol/kg (all P＜0.01)].Compared with balloon injury group,the relative expressions of Ras in three fasudil intervention group were 0.49±0.12,0.39±0.13,0.20±0.23 vs.0.62±0.10(P＜0.05 or,P＜0.01); MEK1/2 0.35±0.14,0.29±0.09,0.11±0.12 vs.0.56±0.1(P＜0.05 or,P＜0.01); ERK1/2 0.48±0.15,0.32±0.08,0.12±0.09 vs.0.64±0.13(P＜0.05 or,P＜0.01).The activations of ERK1/2,MEK1/2 and Ras were strongly suppressed in fasudil treated samples on day 14 after balloon injury.Conclusions Blockade of oxidative stress and Ras MEK1/2 ERK1/2 pathway is involved in the fasudil attenuating of balloon injury-induced neointima hyperplasia

Objective To observe the effect of comfort care on psychological status and life quality of patients undergoing interventional treatment of coronary heart disease. Methods 86 cases patients with coronary heart disease were randomly classified into the observation group and the control group with 43 cases in each group. The observation group was treated with comfort care and the control group was treated with normal nursing. The psychological status and life quality between the two groups were compared. Results Compared with pre-nursing, not only physiological function, independence, spiritual beliefs, social relations and quality of life but also SAS and SDS in the observation group were significantly improved after nursing. However, there were no significant differences in the control group. Moreover, the scores of life quality and anxiety and depression were significantly better than those in the control group. Conclusions Comfort care on patients with coronary heart disease can improve anxiety and depression status, servicing satisfaction and life quality of patients.

Objective To observe the effect of nursing intervention on anxiety and depression status and life quality of patients with chronic heart failure (CHF). Methods 124 cases patients with CHF were randomly classified into the observation group and the control group with 62 cases in each group.The observation group was treated with nursing intervention,the control group was treated with routine nursing.The anxiety and depression status and life quality between the two groups were compared. Results Compared with pre-nursing,not only SAS and SDS score but also physical function,role function,emotional function,social function and quality of life in the observation group were significantly improved after nursing.However,there were no significant differences in the control group.Moreover,the scores of anxiety and depression and life quality were significantly better than those in the control group. Conclusions Nursing intervention for patients with CHF can improve anxiety and depression status and life quality of patients.

Starting on the research findings from a study on cancer patients decision making,the author tried to further analyze the Doctor-Patient-Relationship in China from the historical perspective.Having the disease re-moved or treat the patient responsibly,represents different meaning and implication on how a society regards the medicine as profession,and how define the role of physicians.Considerations on these issues are hoped to facilitate the improvement of doctor-patient relationship in China.

OBJECTIVE:To observe clinical efficacy of clomiphene combined with metformin in the treatment of polycys-tic ovary syndrome. METHODS:112 patients with polycystic ovary syndrome admitted in gynaecology and obstetrics depart-ment of our hospital were selected,and randomly divided into control group and observation group,with 56 cases in each group. Control group was treated with clomiphene orally,since fifth day of menstruation,once a day,50 mg/time;observa-tion group was additionally treated with metformin orally,30 min before meal,3 times a day,500 mg/d. Treatment course last-ed for 2 months. Biochemical indicators,control time of disease,actual treatment time,HOMA-IR,HOMA-β and clinical effi-cacy of patients were observed in two groups. RESULTS:After treatment,LH,FSH,T and FINS of patients in observation group were lower than before treatment and control group,with statistical significance(P<0.05);control time of disease and actual treatment time of observation group were significantly shorter control group (P<0.05),with statistical significance;HOMA-IR and HOMA-β of observation group were significantly lower than those of control group,with statistical significance (P<0.05);clinical effective rate of patients in observation group was significantly higher than control group,with statistical significance (P<0.05). CONCLUSIONS:Clomiphene combined with metformin in the treatment of polycystic ovarian syn-drome can improve biochemical indicators,shorten control time of disease and actual treatment time and enhance clinical effi-cacy.

Based on the peak value of power spectral density (PPSD) and corresponding frequency (CF), an approach that performs electroencephalogram (EEG) feature extraction during imaginary right and left hand movements was proposed. The data were gained from brain computer interface competition in 2003 provided by Graz University of Technology. The EEG signals between 8-16 Hz were decomposed by db3 wavelet packet at three levels. The PPSD and CF of electrodes C3 and C4 were defined as the EEG feature vectors and calculated respectively. The left and right hand motor imaginary tasks were distinguished by the time-variable linear classifier. The proposed method was applied to the test data for 140 trials. The satisfactory results were obtained with the highest classification accuracy 89.29%. The maximum mutual information was 0.622 8 bit, and the signal-to-noise ratio (SNR) was 1.371 3. The PPSD and its CF on electrodes C3 and C4 between 8 and 16 Hz were coincident with event-related desynchronization (ERD) and event-related synchronization (ERS). This method is simple, quick, and promising for on-line brain computer interface system.

Traced back to the one decade's development of research ethics in China,the author summarizes the current issues,such as: not sufficient implementing of informed consent,relatively loose review of IRB,IRBs lack ability to improve the review level and not provide much education opportunities to researchers,and not yet take account into the continuous review to the protocols approved before.By comparing the current issues of China and the conditions of U.S.in 1998,we can find some similarities exist,so we can learn from the lessons and experiences of the development of research ethics in the United and the other country,such as adding the model of region ethical review committee,instead of only institutional IRB.The author aims to point out some general ideas about research ethics capacity building,enforce training continuously,government pay much attention to IRB regulation and consider much on cultural elements,which are all bases for the road of sustainable development of research ethics in China.

Objective To probe into patients' mental and physical requirements,strengthen medical ethics and serve patients with great humanity.Methods A survey with self-designed questionnaire was conducted to find out satisfaction rates and problems concerned with the relationship between nurses and patients and the percentage was dealt with statistics.Results In emotion,satisfaction rates(SR) of inpatients and the patients who had already left hospital were 96.47% and 96.86% respectively;in communication,their SR were 95.88% and 97.45%;in skill,SR were 95.44% and 97.06%;in basic nursing,SR were 94.41% and 95.07%;in patients' comprehensive evaluation of nurses,thier SR were 95.59% and 96.75%.Conclusion Improvement of the relationship between nurses and patietns depends on heightening ethical level of nursing workers,which is the key measure to effectively mobilize initiative and raise working efficiency and nursing quality.

Objective To compare the effect of vaginal and laparoscopic myomectomy.Methods According to the digital table,98 patients with uterine fibroids were randomly divided into the two groups,the control group(n =4 9 eases) and the observation group (n =4 9 cases).The patients in the control group were operated through laparoscopic myomectomy,while the patients in the observation group were operated through vaginal myomectomy.The operative time,blood loss,postoperative gastric function recovery time,exhaust time,complications,recurrence and quality of life,etc.were observed.Results The operative time,blood volume were (115.4 ± 29.4) min,(191.4 ± 30.2) mL in the control group and (70.1 ± 18.2) min,(156.1 ±21.6)mL in the observation group.The operative time,blood volume in the control group were more than those in the observation group,there were significant differences between the two groups (t =3.169 4,2.162 8,P ＜ 0.05).Gastric function recovery and exhaust time were (2.8 ± 0.7) h,(23.1 ±4.1)h in the control group and (1.3 ±0.5)h,(15.1 ±3.5)h in the observation group.Gastric function recovery and exhaust time in the control group were higher than those in the observation group (t =2.091 4,3.771 8,all P ＜ 0.05).The number of cases of complications and recurrence were 1,2 in the control group and 2,1 in the observation group.There was no significant difference between the two groups about the number of cases of complications and recurrence (x2 =2.02,0.00,all P ＞ 0.05).Quality of life after operation was (7.26 ± 2.01) in the control group,and (8.42 ± 2.17) in the observation group.The quality of life in the observation group improved more significantly than the control group (t =0.361 8,3.901 6,all P ＜ 0.05).Conclusion Vaginal myomectomy had less trauma,quicker recovery,and help to improve the quality of life.

Objective To explore the status quo of knowledge,belief,and practice of anticoagulationrelated self-management in rehabilitation period for patients with haeat valves replacement.Methods Van Manen's hermeneutic phenomenology was used as qualitative research method.Twenty patients who had undergone heart valves replacement operation were selected and interviewed.The results of interview were analyzed with category analysis.Results After repeated self-examination,comparison,and summery,four themes were refined,including patients lack anticoagulation-related knowledge and skills in rehabilitation period,the levels of patients' attitudes and beliefs varied greatly,patients' compliance of self-management behavior and taking medicine was low,there were limited channels to get anticoagulation-related information in rehabilitation period.Conclusions More attention should be paid to patients for anticoagulation management after valve replacement operation,especially to those of low literacy,older age,and rural area.Nurses should implement the specific health education about anticoagulation management to patients during discharge guide and following-up period to minimize complications,promote the survival rate,and improve their quality of life.