ObjectiveTo find the mechanism of Heshouwuyin(HSWY) in anti-aging, so as to provide theoretical and experiment evidence for the application of HSWY. Methods50 eight-week female SD rats ( SPF degree)were used in this study. The rats were randomly divided into 5 groups:normal control group, 10 rats; model group,10 rats; HSWY anti-aging group, including low, middle and high dose group,10 rats in each group. The sub-acutely aging rats were made by ip injection of D-galactose for 60 days continually. The rats in HSWY groups were administered with various doses of intragastric HSWYduring D-galactose injection. The parameters of ovary levels of SOD,GSH-PX, TAOC were detected. ResultsThe MDA in ovaries of model rats was significantly higher than that of negative control group;while the SOD, GSH-PX, TAOC of model rats were significantly lower than negative control group.The MDA content in ovaries after taking HSWY was significantly lower than that of model rats; while the SOD, GSHPX,TAOC of the taking HSWY rats was significantly higher than that of model rats. ConclusionThe HSWY could increase antioxida tive activity of rat ovaries to stay D-galactose induced consenescence.

Objective To evaluate the effects of fasudil on neointima hyperplasia in rat carotid artery balloon-injury model,and explore the potential molecular mechanisms of this effect.Methods Totally 30 male Sprague-Dawley (SD) rats were randomly divided into sham operation group,balloon injury group,2.5 ml/kg fasudil intervention group,5 ml/kg fasudil intervention group,10 ml/kg fasudil intervention group.The intima-to media(I/M) area ratio of neointima was calculated at 14th day after operation; the protein expression of proliferating cell nuclear antigen(PCNA) was measured by immunohistochemical and the integrated optical density (IA) was calculated.As the marker of oxidative stress,the levels of 8-hydroxy-2'-dcoxyguanosine (8OHdG) and malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELISA).The expressions of phosphorylated and non phosphorylated Ras,mitogen activated protein kinase kinase (MEK1/2) and extracellular signal regulated kinase (ERK1/2) were measured by Western blot.Results The I/M ratios were (0.22 ± 0.01) in sham operation group,(2.71 ± 0.23) in balloon injury group,( 1.72 ±0.24)in 2.5 ml/kg fasudil intervention group,(1.23±0.11)in 5 ml/kg fasudil intervention group,(0.85±0.12)in 10 ml/kg fasudil intervention group.As compared with sham operation group,I/M area ratio was increased in balloon injury group,and three fasudil intervention group inhibited neointima hyperplasia in a dose-dependent manner (all P＜0.01).The IA indexes were(15.11 ±1.42),(53.42±2.30),(45.25±1.62),(22.55±0.88) and(19.61±0.79) in the above five groups,respectively,showing the fasudil inhibited PCNA expression (all P＜0.01).Fasudil also inhibited the levels of 8-OHdG and MDA in a dose-dependent manner [8OHdG:(54.34±4.50)μg/kg,(106.81 ± 6.67)μg/kg,(94.21 ± 3.34) μg/kg,(65.58 ± 5.13) μg/kg,(51.32 ± 2.34) μg/kg; MDA:(19.94 ± 0.65 ) μmol/kg,( 58.83 ± 2.81 ) μmol/kg,(48.94 ± 1.35 ) μmol/kg,(40.62 ± 3.24 )μmol/kg,( 36.85 ± 0.91)μmol/kg (all P＜0.01)].Compared with balloon injury group,the relative expressions of Ras in three fasudil intervention group were 0.49±0.12,0.39±0.13,0.20±0.23 vs.0.62±0.10(P＜0.05 or,P＜0.01); MEK1/2 0.35±0.14,0.29±0.09,0.11±0.12 vs.0.56±0.1(P＜0.05 or,P＜0.01); ERK1/2 0.48±0.15,0.32±0.08,0.12±0.09 vs.0.64±0.13(P＜0.05 or,P＜0.01).The activations of ERK1/2,MEK1/2 and Ras were strongly suppressed in fasudil treated samples on day 14 after balloon injury.Conclusions Blockade of oxidative stress and Ras MEK1/2 ERK1/2 pathway is involved in the fasudil attenuating of balloon injury-induced neointima hyperplasia

Based on the peak value of power spectral density (PPSD) and corresponding frequency (CF), an approach that performs electroencephalogram (EEG) feature extraction during imaginary right and left hand movements was proposed. The data were gained from brain computer interface competition in 2003 provided by Graz University of Technology. The EEG signals between 8-16 Hz were decomposed by db3 wavelet packet at three levels. The PPSD and CF of electrodes C3 and C4 were defined as the EEG feature vectors and calculated respectively. The left and right hand motor imaginary tasks were distinguished by the time-variable linear classifier. The proposed method was applied to the test data for 140 trials. The satisfactory results were obtained with the highest classification accuracy 89.29%. The maximum mutual information was 0.622 8 bit, and the signal-to-noise ratio (SNR) was 1.371 3. The PPSD and its CF on electrodes C3 and C4 between 8 and 16 Hz were coincident with event-related desynchronization (ERD) and event-related synchronization (ERS). This method is simple, quick, and promising for on-line brain computer interface system.

Fifty-one cases of labyrinthine fistula had been reviewed in 1990-1997 during chronic ear surgery. All cases had a lateral semicircular canal fistula and one case had an additional posterior semicircula canal fistula. There was no relationship between the size of labyrinthine fistula and preoperative bone conduction. 34 cases had chronic otitis media with cholesteatoma,the matrix of the cholesteatoma was completely removed in all patients except one case. Preoperative bone conducton hearing was preserved after removal of cholesteatoma matrix in all cases except the one case.

Traced back to the one decade's development of research ethics in China,the author summarizes the current issues,such as: not sufficient implementing of informed consent,relatively loose review of IRB,IRBs lack ability to improve the review level and not provide much education opportunities to researchers,and not yet take account into the continuous review to the protocols approved before.By comparing the current issues of China and the conditions of U.S.in 1998,we can find some similarities exist,so we can learn from the lessons and experiences of the development of research ethics in the United and the other country,such as adding the model of region ethical review committee,instead of only institutional IRB.The author aims to point out some general ideas about research ethics capacity building,enforce training continuously,government pay much attention to IRB regulation and consider much on cultural elements,which are all bases for the road of sustainable development of research ethics in China.

OBJECTIVE:To observe the changes of mitochondrial membrane potential(MMP)at different time in the process of simvastatin-induced apoptosis of K562 cells.METHODS:K562 cells cultivated by routine method for 24h were treated in the 20? mol? L-1 simvastatin for 12,24,48,and 72h,respectively.And theh cell morphological changes were observed.Vitality of K562 cells was detected with MTT;flow cytometry were performed to detect cell apoptotic ratio and changes of mitochondrial membrane potential,and the detection results were compared with those in solvent control group.RESULTS:As compared with control group,the morphology changes of K562 cells such as karyopyconosis,nuclear fragmentation and apoptotic body emerged after treatment with 20? mol? L-1 simvastatin for 48h;the apoptotic ratio at 12,24,48,and 72h increased by(2.55? 0.35)%,(6.1? 0.35)%,(14.15? 0.42)%,and(30.70? 0.65)%,respectively;and the apoptotic ratio of K562 cells increased time-dependently.The cell membrane potential was decreased by(0.7? 0.24)%,(39.6? 4.80)%,(24.4? 2.45)%,and(6.0? 1.62)%,respectively,with that at 24h showing the highest decreasing rate.CONCLUSION:The change of MMP is the early events of apoptosis.The potential mechanism of K562 cells apoptosis induced by simvastatin might be related to the collapse of MMP.

Objective To probe into patients' mental and physical requirements,strengthen medical ethics and serve patients with great humanity.Methods A survey with self-designed questionnaire was conducted to find out satisfaction rates and problems concerned with the relationship between nurses and patients and the percentage was dealt with statistics.Results In emotion,satisfaction rates(SR) of inpatients and the patients who had already left hospital were 96.47% and 96.86% respectively;in communication,their SR were 95.88% and 97.45%;in skill,SR were 95.44% and 97.06%;in basic nursing,SR were 94.41% and 95.07%;in patients' comprehensive evaluation of nurses,thier SR were 95.59% and 96.75%.Conclusion Improvement of the relationship between nurses and patietns depends on heightening ethical level of nursing workers,which is the key measure to effectively mobilize initiative and raise working efficiency and nursing quality.

Objective To explore the status quo of knowledge,belief,and practice of anticoagulationrelated self-management in rehabilitation period for patients with haeat valves replacement.Methods Van Manen's hermeneutic phenomenology was used as qualitative research method.Twenty patients who had undergone heart valves replacement operation were selected and interviewed.The results of interview were analyzed with category analysis.Results After repeated self-examination,comparison,and summery,four themes were refined,including patients lack anticoagulation-related knowledge and skills in rehabilitation period,the levels of patients' attitudes and beliefs varied greatly,patients' compliance of self-management behavior and taking medicine was low,there were limited channels to get anticoagulation-related information in rehabilitation period.Conclusions More attention should be paid to patients for anticoagulation management after valve replacement operation,especially to those of low literacy,older age,and rural area.Nurses should implement the specific health education about anticoagulation management to patients during discharge guide and following-up period to minimize complications,promote the survival rate,and improve their quality of life.

The results of the arterial blood study in 30 patients of cor pulmonale in acute exacerbation were reported.The study included gas analysis, and dominations of serum lactate,pyruvate, potassium,sodium and chlorine.All the items were determined simultaneously.The data demonstrated that when the arterial oxygen tension of the patients with cor pulmonale in acute exacerbation dropped below 60mmHg, the blood lactate concentration was most likely to show a rise, which was related not only to hypoxemia but also to alkalosis.Observations of the changes in concentration of blood lactate and pyruvate in some patients led us to believe that these changes were associated with the seriousness of the disease. Regular measurement of blood lactate and pyruvate concentrations might be helpful to judge the condition of the patient. Persistent rise in blood lactate concentraion suggested poor prognosis.

Objective:To construct a prokaryotic expression plasmid containing HCVF gene,and purify the recombinant fusion protein in E.coli system. Methods: F gene of Hepatitis C virus was amplified by PCR method from plasmid H/FL(containing full length cDNA sequence of HCV 1a subtype),cloned into pET32a(+)vector,and then transformed into E.coli JM109. After identified by restriction diges- tion and DNA sequencing,recombinant plasmid was transformed into E.coli BL21 and induced with IPTG. The fusion protein trxA- F was further confirmed by Western blot analysis and purified by affinity chromatography method. Results: Restriction digestion and PCR screening showed that HCV F gene was cloned into pET32a(+)successfully. After BL21 was transformed with recombinant vector pET32a (+)- HCVF and induced with 0.5mmol/L IPTG,a 35.4kDprotein band was found bySDS- PAGE. And this recombinant protein showed a highly specific and strong reaction with anti- His monoclonal antibody by Western blot analysis. Conclusion: The prokaryotic expression plasmid pET32a(+)- HCVF was successfully constructed,which will be helpful for further research.