Diabetic ketoacidosis is one of the most common causes of death in the pediatric diabetes,and it is also one of the most common endocrine emergencies in children.With the further studies of this disease,more attentions are paid to the central nervous damage about DKA.The mechanisms about DKA-related cognitive dysfunction from the macro to the micro are constantly updated.This article gives a review on the pathogenesis of the cognitive dysfunction due to DKA.

Objective To observe the influence of parturients hemodynamics and curative effect of preventing hypotension syndrome by using hypotension syndrome prevention bracket in cesarean section with spinal anesthesia.Methods One hundred parturients of cesarean section,ASA grade Ⅰ-Ⅱ,were divided into two groups according to whether the use of hypotension syndrome prevention bracket with 50 cases each:observation group and control group.Control group in anesthesia after the success of the surgery made bed left tilt 15°,and observation group in anesthesia after the success immediate used hypotension syndrome prevention bracket.In the two groups,the change of systolic blood pressure (SBP),diastolic blood pressure (DBP),heart rate (HR),mean arterial pressure (MAP),the incidences of hypotension syndrome and nausea and vomiting during and after operation,and anesthesia to uterine incision time and anesthesia time were observed.Results There was no significant difference in anesthesia to uterine incision time,anesthesia time,intraoperative infusion quantity,amount of bleeding between the two groups (P ＞ 0.05).The incidences of hypotension syndrome and nausea and vomiting in observation group were 8.00％ (4/50) and 4.00％ (2/50),in control group were 42.00％ (21/50) and 24.00％ (12/50),there were significant differences between the two groups (P ＜ 0.05).The observation group at 5 min after anesthesia SBP,DBP,MAP were (106.12 ± 12.91),(57.26 ± 11.43),(73.57 ± 11.03) mm Hg (1 mm Hg =0.133 kPa),and the control group were (95.44 ± 19.53),(50.15 ± 15.53),(66.36 ± 15.64) mm Hg,there were significant differences between the two groups (P ＜ 0.05).The observation group at 10 min after anesthesia SBP,DBP,MAP were (104.35 ± 11.86),(55.92 ± 10.81),(72.54 ± 10.37) mm Hg,the control group were (102.31 ± 15.48),(55.12 ± 11.89),(70.72 ± 11.33) mm Hg,there were significant differences between the two groups (P＜ 0.05).The observation group at 3 min after the delivery of fetus and the end of operation DBP,MAP were (54.13 ± 9.14) and (62.63 ± 8.61) mm Hg,(68.13 ± 10.40) and (80.88 ± 9.05) mm Hg,the control group were (49.24 ± 11.55) and (58.04 ± 11.67) mm Hg,(70.57 ± 8.58) and (75.35 ± 10.41) mm Hg,there were significant differences between the two groups (P ＜ 0.05).Conclusion Cesarean section parturients using hypotension syndrome prevention bracket can reduce the occurrence of hypotension syndrome,which can help to maintain the stability of parturients hemodynamics,it is worth of clinical promotion.

Objective To investigate the incidence rate and risk factors of obstetric emergency hysterectomy.Methods The clinical data of childbirth puerpera in Shangqiu four municipal hospitals were retrospectively analyzed,80 patients underwent routine obstetric emergency hysterectomy were selected as hysterectomy group,56 postpartum hemorrhage patients without uterus excision were randomly selected as control group.The related risk factors of obstetric emergency were analyzed.Results (1) The total delivery number was 65 259 cases,cesarean rate was 47.46％ (30 972/65 259),postpartum hemorrhage rate was 4.61％ (1 429/30 972),and uterus removal rate of cesarean section was 0.18％ (56/30 972),vaginal delivery postpartum hemorrhage rate was 3.28％ (1 124/34 287),in the courtyard the vaginal delivery emergency uterus removal rate was 0.03％ (12/34 287).The postpartum hemorrhage rate and obstetrics emergency uterus removal rate were higher than vaginal delivery,the differences were statistically significant (x2 =77.22,33.24,all P ＜ 0.05).(2) The results of the single factor analysis showed that,placenta previa and implant,placental abruption,merge multiple or huge uterine fibroids,uterine rupture,blood coagnlation dysfunction,armount of bleeding ＞ 2 000ml,vanda operation with oxytocin rate between the two groups,there were significant differences(P ＜ 0.01).(3) The results of unconditional multi-factor Logistic regression analysis showed that,placental abruption,uterine rupture,merge multiple or huge uterine fibroids,blood coagulation dysfunction,amount of bleeding ＞ 2 000ml were independent risk factors which lead to obstetric emergency hysterectomy.Vanda operation with oxytocin and hysterectomy had negative correlation.Conclusions Obstetric emergency hysterectomy was related with a variety of factors.Strengthen the perinatal health care,and actively prevent emergency hysterectomy can reduce the dangerous factors of uterus removal rate and has important significance.

Objective To observe the effect of comfort care on psychological status and life quality of patients undergoing interventional treatment of coronary heart disease. Methods 86 cases patients with coronary heart disease were randomly classified into the observation group and the control group with 43 cases in each group. The observation group was treated with comfort care and the control group was treated with normal nursing. The psychological status and life quality between the two groups were compared. Results Compared with pre-nursing, not only physiological function, independence, spiritual beliefs, social relations and quality of life but also SAS and SDS in the observation group were significantly improved after nursing. However, there were no significant differences in the control group. Moreover, the scores of life quality and anxiety and depression were significantly better than those in the control group. Conclusions Comfort care on patients with coronary heart disease can improve anxiety and depression status, servicing satisfaction and life quality of patients.

Objective To observe the effect of nursing intervention on anxiety and depression status and life quality of patients with chronic heart failure (CHF). Methods 124 cases patients with CHF were randomly classified into the observation group and the control group with 62 cases in each group.The observation group was treated with nursing intervention,the control group was treated with routine nursing.The anxiety and depression status and life quality between the two groups were compared. Results Compared with pre-nursing,not only SAS and SDS score but also physical function,role function,emotional function,social function and quality of life in the observation group were significantly improved after nursing.However,there were no significant differences in the control group.Moreover,the scores of anxiety and depression and life quality were significantly better than those in the control group. Conclusions Nursing intervention for patients with CHF can improve anxiety and depression status and life quality of patients.

ObjectiveTo find the mechanism of Heshouwuyin(HSWY) in anti-aging, so as to provide theoretical and experiment evidence for the application of HSWY. Methods50 eight-week female SD rats ( SPF degree)were used in this study. The rats were randomly divided into 5 groups:normal control group, 10 rats; model group,10 rats; HSWY anti-aging group, including low, middle and high dose group,10 rats in each group. The sub-acutely aging rats were made by ip injection of D-galactose for 60 days continually. The rats in HSWY groups were administered with various doses of intragastric HSWYduring D-galactose injection. The parameters of ovary levels of SOD,GSH-PX, TAOC were detected. ResultsThe MDA in ovaries of model rats was significantly higher than that of negative control group;while the SOD, GSH-PX, TAOC of model rats were significantly lower than negative control group.The MDA content in ovaries after taking HSWY was significantly lower than that of model rats; while the SOD, GSHPX,TAOC of the taking HSWY rats was significantly higher than that of model rats. ConclusionThe HSWY could increase antioxida tive activity of rat ovaries to stay D-galactose induced consenescence.

Objective To evaluate the effects of fasudil on neointima hyperplasia in rat carotid artery balloon-injury model,and explore the potential molecular mechanisms of this effect.Methods Totally 30 male Sprague-Dawley (SD) rats were randomly divided into sham operation group,balloon injury group,2.5 ml/kg fasudil intervention group,5 ml/kg fasudil intervention group,10 ml/kg fasudil intervention group.The intima-to media(I/M) area ratio of neointima was calculated at 14th day after operation; the protein expression of proliferating cell nuclear antigen(PCNA) was measured by immunohistochemical and the integrated optical density (IA) was calculated.As the marker of oxidative stress,the levels of 8-hydroxy-2'-dcoxyguanosine (8OHdG) and malondialdehyde (MDA) were measured by enzyme-linked immunosorbent assay (ELISA).The expressions of phosphorylated and non phosphorylated Ras,mitogen activated protein kinase kinase (MEK1/2) and extracellular signal regulated kinase (ERK1/2) were measured by Western blot.Results The I/M ratios were (0.22 ± 0.01) in sham operation group,(2.71 ± 0.23) in balloon injury group,( 1.72 ±0.24)in 2.5 ml/kg fasudil intervention group,(1.23±0.11)in 5 ml/kg fasudil intervention group,(0.85±0.12)in 10 ml/kg fasudil intervention group.As compared with sham operation group,I/M area ratio was increased in balloon injury group,and three fasudil intervention group inhibited neointima hyperplasia in a dose-dependent manner (all P＜0.01).The IA indexes were(15.11 ±1.42),(53.42±2.30),(45.25±1.62),(22.55±0.88) and(19.61±0.79) in the above five groups,respectively,showing the fasudil inhibited PCNA expression (all P＜0.01).Fasudil also inhibited the levels of 8-OHdG and MDA in a dose-dependent manner [8OHdG:(54.34±4.50)μg/kg,(106.81 ± 6.67)μg/kg,(94.21 ± 3.34) μg/kg,(65.58 ± 5.13) μg/kg,(51.32 ± 2.34) μg/kg; MDA:(19.94 ± 0.65 ) μmol/kg,( 58.83 ± 2.81 ) μmol/kg,(48.94 ± 1.35 ) μmol/kg,(40.62 ± 3.24 )μmol/kg,( 36.85 ± 0.91)μmol/kg (all P＜0.01)].Compared with balloon injury group,the relative expressions of Ras in three fasudil intervention group were 0.49±0.12,0.39±0.13,0.20±0.23 vs.0.62±0.10(P＜0.05 or,P＜0.01); MEK1/2 0.35±0.14,0.29±0.09,0.11±0.12 vs.0.56±0.1(P＜0.05 or,P＜0.01); ERK1/2 0.48±0.15,0.32±0.08,0.12±0.09 vs.0.64±0.13(P＜0.05 or,P＜0.01).The activations of ERK1/2,MEK1/2 and Ras were strongly suppressed in fasudil treated samples on day 14 after balloon injury.Conclusions Blockade of oxidative stress and Ras MEK1/2 ERK1/2 pathway is involved in the fasudil attenuating of balloon injury-induced neointima hyperplasia

Objective:To investigate the changes of reactive oxygen species(ROS)and Glutathione(GSH) and their chronological order in the apoptosis of K562 cells induced by simvastatin to explore the apoptosis mechanism.Methods:K562 cells cultivated in routine method for 24h were exposed in the simvastatin 20?mol/L.Cell morphological analysis were conducted at 48h and the vitalities of K562 cells were cultivated detected with MTT at different times(12,24,48,72 h) respectively.Fluorochrome flow cytometry was performed to detect cell apoptotic ratio and changes of intracellular ROS.The content of intracellular GSH in K562 cells was measured by colorimetric method.Results:K562 cells presented typical apoptosis morphological changes the treatment of simvastatin for 48h.cell inhibition ratio at different times(12,24,48,and 72 h) were 0,(19.02?0.92)%,(56.4?3.20)%,(74.4?5.54)% respectively and cell growth of K562 was inhibited.The apoptotic ratio were(2.55?0.25)%,(6.1?0.35)%,(14.15?0.42)%,and(30.70?0.65)% respectively.Furthermore,the changes of fluorescence intensity of ROS were also significantly increased(P

Objective To compare the effect of vaginal and laparoscopic myomectomy.Methods According to the digital table,98 patients with uterine fibroids were randomly divided into the two groups,the control group(n =4 9 eases) and the observation group (n =4 9 cases).The patients in the control group were operated through laparoscopic myomectomy,while the patients in the observation group were operated through vaginal myomectomy.The operative time,blood loss,postoperative gastric function recovery time,exhaust time,complications,recurrence and quality of life,etc.were observed.Results The operative time,blood volume were (115.4 ± 29.4) min,(191.4 ± 30.2) mL in the control group and (70.1 ± 18.2) min,(156.1 ±21.6)mL in the observation group.The operative time,blood volume in the control group were more than those in the observation group,there were significant differences between the two groups (t =3.169 4,2.162 8,P ＜ 0.05).Gastric function recovery and exhaust time were (2.8 ± 0.7) h,(23.1 ±4.1)h in the control group and (1.3 ±0.5)h,(15.1 ±3.5)h in the observation group.Gastric function recovery and exhaust time in the control group were higher than those in the observation group (t =2.091 4,3.771 8,all P ＜ 0.05).The number of cases of complications and recurrence were 1,2 in the control group and 2,1 in the observation group.There was no significant difference between the two groups about the number of cases of complications and recurrence (x2 =2.02,0.00,all P ＞ 0.05).Quality of life after operation was (7.26 ± 2.01) in the control group,and (8.42 ± 2.17) in the observation group.The quality of life in the observation group improved more significantly than the control group (t =0.361 8,3.901 6,all P ＜ 0.05).Conclusion Vaginal myomectomy had less trauma,quicker recovery,and help to improve the quality of life.