1.Effect of Lidan Bushen Granules on acetylcholine and excitatory amino acids of hippocampus in saturnine rats
Yalei WANG ; Rong MA ; Xilian ZHANG ; Linlin LIU ; Wenman JIN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(11):-
Objective: To discuss the neurobiochemical mechanism of Lidan Bushen Granules in treating saturnine children. Methods: Saturnine rats model was established by intragastric, and was treated with Lidan Bushen Granules for 5 weeks. The acetylcholine and excitatory amino acids of hippocampus in saturnine rats were measured. Results: Compared with the normal group, the ACH and EAA contents of hippocampus in model group rats were dramatically lower (P
2.Analysis of suspicious results of serum HBV DNA detected by fluorescence quantitative PCR
Zhanguo CHEN ; Wu ZHOU ; Zhongyong WANG ; Yalei JIN ; Zhihua TAO
Chinese Journal of Laboratory Medicine 2013;(3):217-221
Objective To analyze the suspicious results of serum HBV DNA by fluorescence quantitative PCR and develop appropriate countermeasures in order to improve the quality of detection of HBV DNA.Methods Blood samples of patients from the First Affiliated Hospital of Wenzhou Medical College from 2008 to 2011 were analyzed for HBV DNA by fluorescence quantitative PCR.1969 cases of suspicious results,judged by the rule of review the results of serum HBV DNA combined with the historical results,PCR amplification curve,HBV serum markers and clinical diagnosis,were analyzed and redetected by using of two different reagents,careHBV PCR Kit and careHBV PCR Kit V2,at the same time.The consistency and inconsistency ratio of the results were evaluated.Both the reasons of inconsistent and the undetected rates of careHBV PCR Kit were analyzed.The two reasons for the inconsistent results included the reagent related factors,e.g,showing no amplification curve caused by the false negative and abnormal low efficiency of amplification curve,and the non reagent related factors such as operating pollution and other sample factors.Results There were 115 154 blood samples were detected for HBV from 2008 to 2011 and 1969 samples (1.71%) with suspicious results were redetected.The consistency and inconsistency results were 1588 (80.65%) and 381 (19.35%),respectively.Every year from 2008 to 2011,the percentage of the inconsistent results caused by the reagent related factors were 18.87%,20.23%,51.33% and 59.57% respectively,which showed an increasing trend,and the percentage of inconsistent results caused by the nonreagent related factors were 81.13%,79.77%,48.67% and 40.43% respectively,which showed a declining trend year by year.The undetected rates of careHBV PCR Kit were 2.49%,4.08%,10.09% and 14.47% respectively,showing an increasing trend.Conclusions The redetection for the specimens with the suspicious results by using of different reagents can avoid the blind detection of HBV DNA and reduce the experimental error.All the clinical samples for quantitative HBV DNA including the mutations of HBV gene can be measured accurately and effectively,which is helpful to hepatitis B patients for antiviral therapy.
3.Analysis of food contamination by lead, cadmium, mercury, copper and aluminum in Ningxia
Qiuning WEI ; Boxuan JIN ; Yalei DING ; Tao CHEN
Chinese Journal of Endemiology 2015;34(5):349-352
Objective To investigate the situation of food contamination by lead,cadmium,mercury,copper and aluminum in Ningxia Region.Methods Ten categories of food samples up to 712 copies including dairy,edible mushrooms,eggs,preserved egg,meat,organs,aquatic products,vegetables,grains and pasta from supermarkets of five administrative cities of Ningxia Autonomous Region were selected in 2010.The contents of lead,cadmium and mercury in food were detected in 646 samples of 9 categories of food,and copper content of the 30 meat samples were detected.Aluminum content in 66 samples of flour food was tested.According to the methods of national food safety standards (GB 5009.12-2010) and national food hygiene standards (GB/T 5009.13-2003,GB/ T 5009.15-2003 and GB/T 5009.17-2003),lead,copper,cadmium and mercury in various foods were tested.Aluminum was determined using the method for determination of spectrophotometry.Results The detection rates of lead,cadmium,mercury in the nine categories of food were 52.8% (341/646),62.1% (401/646),51.7% (334/646);the detection rates of copper in the meat was 90.0% (27/30);the aluminum in flour food was 84.8% (56/66),respectively.Compared with the national standards,the eligible rates of lead,cadmium,mercury,copper and aluminum were 97.1% (627/646),98.2% (539/549),100.0% (646/646),100.0% (30/30) and 75.8% (50/66),respectively.Food contaminated by lead and cadmium were mainly distributed in preserved egg [lead detection rate 9.5% (2/21)],organs [lead detection rate 6.7% (2/30),cadmium detection rate 30.0% (9/30)],vegetables [lead detection rate 9.9% (8/90)] and a small amount of food products [lead detection rate 4.7% (7/150)].The levels of lead in fried flour food and fine dried noodles were higher [40.9% (9/22),36.4% (4/11)].Conclusions Food contamination by lead,cadmium,mercury and copper is not serious in Ningxia.The monitoring results of lead,cadmium,mercury and copper in food are satisfying in Ningxia.But there is aluminum contamination in flour food.It is important to do the risk evaluation of aluminum contamination in food as quickly as possible.
4.Determination of 38 Kinds of Pesticide Residues in Nuts by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
Yalei DONG ; Wenjing LIU ; Jin CAO ; Gangli WANG
Chinese Journal of Analytical Chemistry 2017;45(9):1397-1404
A multi-residue analysis method was developed for the determination of 38 kinds of pesticides in nuts (almonds, peanuts, cashew nuts and walnuts) by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry.The pesticide residues were extracted with acetonitrile.The extract was cleaned up with PSA, C18 and Oasis PRiME HLB, and then analyzed by UPLC-MS/MS with multiple reaction monitoring (MRM) mode.External standard method was employed to quantify.The limits of detection (LODs, S/N=3) of this method were between 0.01 and 10 μg/kg, and the limits of quantitation (LOQs, S/N=10) were between 0.05-20 μg/kg.All of the tested pesticides showed good linear relationship (r>0.991).The practical samples were determined at three spiked levels and the average recoveries were between 51.0% and 126.0%.The RSDs were less than 20%.This method was simple, sensitive and accurate, and could be used for the routine analysis of pesticide residues in nuts.
5.Evaluation of anticoagulant efficacy and safety for domestic bivalirudin during percutaneous coronary intervention in elderly patients with acute coronary syndrome
Meng ZHANG ; Na LI ; Lei WANG ; Yalei HAN ; Dongdong ZHAI ; Weihua JIN ; Bin WANG
Chinese Journal of Interventional Cardiology 2014;(5):318-321
Objective To evaluate the safety and efficacy of bivalirudin during perioperation of percutaneous coronary intervention (PCI) in patients older than 80 years old with acute coronary syndrome. Methods A total of 64 patients were randomly divided into two groups, respectively received heparin(n=32), or bivalirudin (n=32). We compared the activated coagulation time (ACT), procedural success rate, bleeding rate between two groups. Results The two groups ACT, PCI success rates are not statistically different, No signiifcant difference in the incidence of mild hemorrhage which is 4 (12.5%) for heparin and 1(3.1%) for bivalirudin and severe bleeding which is 2 (6.2%) for heparin and 0 for bivalirudin. However there are signiifcant differences in the overall incidence of bleeding between the two groups with lower incidence of beeding in the bivalirudin group than the heparin group (P<0.05). Conclusions Bivalirudin has comparable anticoangulation effect as heparin during perioperation of percutaneous coronary intervention (PCI) among patients older than 80 years of age with acute coronary syndrome with lower bleeding incidence than heparin dose.
6.Determination of antioxidant activity of Polygoni Multiflori Radix Praeparata by HPLC-DPPH
Jinzhu QIAN ; Ming LIANG ; Yi YU ; Yalei DENG ; Shijia LIU ; Huiming PANG ; Jin QI
Journal of China Pharmaceutical University 2022;53(2):185-191
An on-line HPLC-DPPH system was developed to determine the antioxidant activity of 16 batches of Polygoni Multiflori Radix Praeparata. By analyzing the chromatographic and biological activity fingerprints of 16 batches of Polygoni Multiflori Radix Praeparata, the dose-effect relationship was established and the total antioxidant activity was quantified by activity addition.The results suggested that the online HPLC-DPPH method can evaluate the antioxidant activity of different bathches of Polygoni Multiflori Radix Praeparata, with different processing methods, aiming to provide datasupport and scientific basis forquality evaluation of Polygoni Multiflori Radix Praeparata.
7.Diagnosis of intestinal perforation on postmortem imaging
Wenju JIN ; Zhiyuan XIA ; Haibin SHEN ; Yalei YU ; Beibei LIU ; Jiayuan GU ; Zhiji HE ; Yu ZHANG ; Sijie LIU ; Xilian DING ; Yuqiang ZHANG ; Qun GONG ; Guanglong HE
Chinese Journal of Forensic Medicine 2023;38(6):660-663
Objective To study the value of postmortem imaging on the diagnosis of intestinal perforation.Method Postmortem imaging(PMCT and PMCTA)data of 2 intestinal perforation deaths(and 4 controlled cases)were reviewed retrospectively.Diagnosing capacities of intestinal perforation by postmortem imaging method were further investigated.Results PMCT is sensitive in detecting the free air and liquid induced by intestinal perforation.PMCT can sometimes detect the gravity-dependent purulent secretions in the abdominopelvic cavity.PMCTA can visualize the extravasation of contrast agent from the perforation,which can be used to locate the accurate perforation region.Conclusion Postmortem imaging method(PMCT and PMCTA)is an important tool for the diagnosis of intestinal perforation,which can not only be used as a forensic diagnosis method,but is also useful to locate the perforation site before an forensic autopsy.