Objective:To investigate the efficacy of low-dose thyroid hormone treatment and the change in levels of thyroid hormone in patients with chronic kidney disease. Methods: 92 children with chronic kidney disease were randomly divided into thyroxine treatment group and control group, with 46 children in each group. All patients received conventional treatment, the treatment group served with additional Levothyroxine of 25 ~ 50?g/d. At least 1 month follow-up was taken to compare the clinical efficacy, RIA detection level of thyroxine. Results: In control group ,urinary protein, urine occult blood, Hb and albumin showed no changes at 1w-4w after therapy, and the treatment group also showed this trend. There were no difference between two groups. However, 1 week after treatment, SCr and BUN began to decline, till 4th week(P

Objective To analyze the use of narcotic drugs and the first class of psychotropic drugs in inpatient pharmacy of Wuhan Red Cross Hospital from 2011 to 2015.Methods Using the defined daily dose (DDD) which recommended by WHO as an index, and the dispensatory and clinical routine dose recommended by Chinese Pharmacopoeia and New Materia Medica as references.Adopting DUI as the statistic index, the drug's variety, quantity, sums and use frequency of the narcotic drugs and the first psychotropic drugs in inpatient pharmacy of our hospital from 2011 to 2015 were analyzed.Results According to the results, there was an increasing trend in sales amount from 2011 to 2015,among them,4 kinds of injections, 2 kinds of oral tablets,1 kind of transdermal patch and 1 kind of the sustained-release tablets.The sales amount of narcotic drugs showed an upward trend year by year.Conclusion In the past 5 years,the use of narcotic drugs and the first class of psychotropic drugs in inpatient pharmacy of the hospital are basically rreasonable, but it still needs to continuously strengthen the knowledge management of narcotic drugs and the related training, so as to ensure safe and effective use of medication in patients.

Objective To investigate the relationship of the occurrence of sinusitis with nasal septum devia-tion.Methods 102 patients with nasal septum deviation confirmed by spiral CT and sinus endoscopy were included. The coronal CT images of all cases were observed.The incidence of sinusitis in different type of nasal septum deviation was calculated and the correlation between sinusitis with nasal septum deviation was analyzed.Results Among 102 patients with nasal septum deviation,deviated septum at high position in 71 cases,deviated septum beyond high position in 31 cases.Sinusitis was detected in 67 cases,accounted for 65.6%,of which unilateral involving in 38 cases,bilateral involving in 29 cases.Main lesions in the nasal septum deviation concave side in 46 cases (68.7%),in nasal septum convex side in 12 cases (17.9%).Conclusion Deviated septum is an important factor in onset.Upper septum deviation and deviated concave side more likely arise sinusitis.

As hepatic fibrosis and cirrhosis have chronic progressive process,the assessment of fibrosis is of great significance for making appropriate treatment.It is divided into two categories,which includes invasive and noninvasive assessing methods.As a noninvasive method,magnetic resonance shows prospect in many aspects.This article is a review of magnetic resonance imaging in the assessment of hepatic fibrosis and cirrhosis.

Objective Galangin is a natural flavonoid with antineoplastic activity .SIRT1 is an important member of Sirtuin family which parcitipate in many physiological process .The aim of this study was to investigate the effect of SIRT 1 on HepG2 cell apop-tosis induced by galangin . Methods HepG2 cells were pre-treated with SIRT1 inhibitor EX-527 for 2 hours, and then galangin for 24 hours.DMSO solvent control group, EX-527 treatment group, galangin treatment group and EX-527 and galangin co-treatment group were established.Hoechst 33342 staining, flow cytometry and western blot were performed to detect the apoptosis of HepG2 cells.After regu-lating the expression of SIRT1 in HepG2 cells with RNA interference and transfection of exogenous genes , these cells were treated with ga-langin for 24 hours.Negative control group , vector control group , SIRT1 knock down group , blank control group , blank vector group ,and SIRT1 upregulation group were established .Western blot and Flow cytometry were performed to detect the apoptosis of HepG 2 cells. Results The apoptosis rate and the gray level ratio of shear band of PARP 1 and GAPDH that of galangin group [(11.62± 0.55) %, 0.89±0.01]and EX-527+galangin group[(25.75±0.61) %, 1.15±0.06] were all increased(P<0.01),when these were compared with DMSO solvent control group [(2.49±0.22) %, 0.06±0.00];and those in EX-527+galangin group were also markedly increased compared with galangin group (P<0.01).The result of western bolt was that the gray level ratio of PARP 1 and GAPDH of SIRT1 knocked down group(0.06±0.01) was markedly decreased compared with vector control group (1.11±0.05)and without adenovi-rus infection group (1.10±0.04)(P<0.01).The apoptosis rate and the gray level ratio of shear band of PARP 1 and GAPDH that of SIRT1 knocked down group were markedly increased compared with vector control group and without adenovirus infection group ( P<0.01).The gray level ratio of PARP1 and GAPDH of SIRT1 up-regulated group (1.63±0.04) was markedly increased compared with blank control group (0.89±0.02) and without plasmid transfection group (0.87±0.03) (P<0.01).The apoptosis rate and the gray lev-el ratio of shear band of PARP 1 and GAPDH that of SIRT1 up-regulated group were markedly decreased compared with blank control group and without plasmid transfection group (P<0.01). Conclusion SIRT1 inhibited galangin-induced apoptosis in HepG2 cells.

anti p62 2 in patients with cancers All of them were lower than that of autoantibody against full length p62 The frequency of autoantibody to full length p62 was significantly higher than that of anti p62 2 and anti p62 3, but no difference with anti p62 1 Totally positive rates of autoantibody against the three fragments were lower than those of full length p62 in patients with hepatocellular cancer, gastric cancer, colorectal cancer, esophagus cancer and breast cancer but with lung cancer Conclusion:Antigenic sites in p62 were mainly located in p62 1 and the integrity of p62 structure is important for antigenic expression

In order to investigate the affinity of aptamers to Bacillus anthracis spore, a custom synthesized 78 mer random DNA library was subjected to 15 rounds of selection against spores of vaccine strain A.16R by using SELEX method. The selected aptamers were cloned and sequenced. Macaw 2.05 and DNAsis 2.5 package were employed to analyze the conserved sequences and second structure of the aptamers, respectively. Affinities of aptamers to the spores were visualized by biotin streptavidin horseradish peroxidase system. The results showed that affinities of the aptamers were different. The highest OD at 450nm was 1.2, and the lowest was 0.25. The second structure analysis revealed possible stem loops for binding to the spores. The conserved sequences, AGGGG, CCCCG, GGGTT and ACACT, were found and the aptamers having same conserved sequence demonstrated similar affinity to the spores.

ObjectiveTo investigate the diagnosis and operative procedure for spontaneous perforation of congenital choledochal cyst in children. MethodsThe clinical data of 16 cases with spontaneous perforation of congenital choledochal cyst were analysed retrospectively.ResultsAll cases were under 4 years old with a mean age of 23 months. Abdominal distension, pain, vomiting, fever and diffuse tenderness were present in all patients. The preoperative diagnosis was established by paracentesis in ten patients. Abdominal sonography and CT found a cyst in 5 of the 10 cases reviewed. In 11 cases (68.7%), the site of perforation was found on the confluence of common bile duct and gallbladder cystic duct. T-tube drainage was used as a transition measure. All patients recovered uneventfully. Radical resection was performed in about three months after the initial operation. ConclusionsThe obstruction of the bile duct and poor blood supply to the choledochal cyst are major causes leading to perforation. Routine abdominal paracentesis should be carried out for a small child with peritonitis and general abdominal tenderness, especially on the right upper quadrant.T-tube drainage should be adopted as an emergent procedure to tide the patient over the crisis followed by elective choledochal cyst resection and bile duct reconstruction.

Objective To investigate the role of klotho protein in the pathogenesis of hypertension. Methods We collected blood samples of 104 patients with hypertension and 61 people without hypertension admitted to our hospital between March and June 2009. Klotho protein and nitric oxide (NO) in blood serum were detected by ELISA and nitro-reductase methods. Results Klotho protein absorbance and NO were lower in hypertension group than in non-hypertension group (P