Aim To develop a molecular biologic technique for detection of chlamudia pneumoniae with nested polymeyase chain reaction (nPCR) .Methods Nested primers were synthesized according to a cloned C.pneumoniae 474- bp Pst I fragmert. Results The 378bp DNA fragments were amplified from C. pneuomoniae with nPCR. None of the C. trachomatis, C. psittaci, other organisms and etc. Strains tested were amplified by the nPCR. The direct sequening of 3 sample products with nPCR are quite same as C. pneumoniae (CWL-29 ) . The sensitivity of nPCR is higter than PCR. Conclusions This method is not only sensitive.specific and rapid, but also provides an etiological basis for cdiagnosis of C.pneumoniae infection.

Objective To investigate the urinary tract infection by Mycoplasma in systemic lupus erythematosus (SLE) patients. Methods Mycoplasma hominis (Mh) and Ureaplasma urealyticum (Uu) from serum, eye, pharynx and urethra secretions were detected by nested polymerase chain reaction (nPCR) in 129 SLE patients, and DNA sequences of positive product were detected and analysed. Results Seventy-nine were positive and 50 were negative, 50 of 79 positive subjects (63.2%) were urinary tract infection, and 11 of 50 negative subjects (22.0%) were infected (P=0.001). Ninty-five active SLE patients, 66(69%) were positive and 29(30.5%) were negative for mycoplasma infection. Thirty-four inactive SLE patients, 13(38%) were positive and 21(61.7%) were negative for mycoplasma infection (P=0.000). Comparing these two setsof patients, the urinary tract infection in the active SLE was significantly higher than in stable patients. Conclusion Mycoplasma infection is the major pathogen inducing SLE flare and may be one of the infective pathogene of SLE.

OBJECTIVE To discuss the risk factors of professional exposure of dental health personnel and treatment measures. METHODS The management system of standardization,systematization,science and universal prevention were carried out and realized to maximally prevent the onset of dental hospital infection. RESULTS A set of feasible management system and measures about professional exposure for dental health personnel were drafted to make dental staff master the management standards of dental infection,realize the sterilization procedures of dental device and prevent dental infection maximally in dental hospital by implementing universal preventive measures during dental treatment. CONCLUSIONS The preventive measures and programs about professional exposure for dental health personnel must be structuralized.

Objective To compare the effect of water-infusion colonoscopy and conventional air-infusion colonoscopy on the bowel cleanliness during withdrawing.Methods A single blind randomized controlled trial was conducted. 222 patients were randomly divided into water-infusion group (group A, 112 cases) and air-infusion group (group B, 110 cases). The cleanliness scores, scores improvement, pain scores, time of reaching cecum, depth of colonoscopy, rate of whole colon examination, adenoma detection rate (ADR), operator’s dififculty and complications were compared between the two groups.Results The cleanliness score during withdrawing was higher in group A [M(8)/IQR(1) vs M(8)/IQR(2),P = 0.000], the improvement was higher in group A [(0.53 ± 0.74) vs (0.23 ± 0.55), P = 0.000], the abdominal pain score was signiifcantly lower in group A [M(2)/IQR(1) vs M(4)/IQR(2),P = 0.000] and the ADR was higher in group A (36.61 % vs 23.64 %,P = 0.041). The operator’s dififculty evaluation score was signiifcantly lower in group A [M(1)/IQR(1) vs M(2)/IQR(1),P = 0.005]. There were no signiifcant differences at the time of reaching cecum, the depth of colonoscopy and the whole colon examination rate between the two groups.Conclusion Water-infusion colonoscopy could signiifcantly improve the bowel cleanliness during withdrawing scope, improve the ADR and reduce abdominal pain of patients, without increasing the time to reach cecum.

OBJECTIVE To investigate the antibiotics-resistant genes in enterococci isolated from the 98th Hospital of PLA,Huzhou,Zhejiang Province,China.METHODS The antibiotics-resistant genes of TEM,aac(6′)/aph(2″),aph(3′)-Ⅲ,ant(2″)-Ⅰ,ant(4′,4″),ant(6)-Ⅰ,ermB,mefA,tetM,vanA,and vanB were analyzed by polymerase chain reaction(PCR) and verified by DNA sequencing in the 15 isolates of Enterococcus faecalis and 9 isolates of E.faecium.RESULTS The positive rate of the resistance genes of TEM,aac(6′)/aph(2″),aph(3′)-Ⅲ,ant(2″)-Ⅰ,ant(4′,4″),ant(6)-Ⅰ,(ermB,) mefA,tetM,vanA,and vanB in the 24 strains of enterococci tested were 37.5%,70.8%,25.0%,0.0%,0.0%,41.7%,75.0%,0.0%,41.7%,4.2%,and 4.2%,(respectively.) CONCLUSIONS The multidrug resistance of enterococci was a serious issue,and harbored antibiotics-resistance genes were the very important reasons of resistance to antibiotics in enterococci.