Objective To explore the distribution of pathogens in lower respiratory tract infection, and to explore the drug resistance, and summarize the clinical experience.MethodsFrom February 2014 to August 2016 in Changxing county people's hospital, the specimens of the patients with lower respiratory tract infection were analyzed.Distribution of pathogenic bacteria was analyzed and its drug resistance was summarized.ResultsA total of 100 strains of bacteria were isolated, including 27(27%) strains of fungi, 26(26%) strains of gram positive bacteria, 47(47%) strains of gram negative bacteria.The proportion of gram negative bacteria was significantly higher than that of gram positive bacteria and fungi, and the difference was statistically significant (P<0.05).Different pathogenic bacteria have different drug resistance to commonly used antibiotics.The resistance of Candida albicans to fluconazole was 100.0%, the resistance of Staphylococcus aureus to compound sulfamethoxazole was 90% and to gentamicin was 100.0%, the resistance of Escherichia coli to levofloxacin was 100.0%.ConclusionGram negative bacteria accounted for the highest proportion in pathogen of lower respiratory tract infection.Different pathogenic bacteria have different resistance to different antibiotics in clinical, and monitoring and rational use of drugs should be strengthened in order to improve the efficacy and reduce drug resistance.

OBJECTIVE To investigate the influence of tea polyphenol in alcoholic liver(hurted) rat by observing its blood plasma endotoxin level,and to provide the direction of new(treatments) and means for the prevention of alcoholic liver disease.METHODS Thirty S-D rats(15 female,15 male) were divided into five groups randomly(noted as A,B,C,D,and E,with equal male and female),and fed in the cleanliness class laboratory.Excepting group E which was given saline,all other groups were given alcohol(56% Hongxing spirits) with the dosage of 7 g/kg once everyday.At the same time,rats in groups B,C and D were given tea polyphenol with the dosage of 0.05,0.125,0.25 g/kg.In the end of the fourth week,got the rats vein blood to determinae serum ALT,AST level and blood plasma endotoxin level.RESULTS The serum AST of the group A was higher than other 4 groups;the blood plasma endotoxin level in groups B,C,and D were(consequently) lower than in group A.CONCLUSIONS The tea polyphenol could reduce the blood plasma endotoxin level in alcoholic liver hurted rat.Its possible mechanism is to repress the isotopy of bowel flora,and to maintain the normal function of bowel mucous membrane.This research provides a new way for curing the alcohol liver hurt.

OBJECTIVE:To study the influence of Dieda paste application on expressions of CD16/32and CD80of mouse macrophages in traumatic condition.METHODS:The mice were divided into3groups:treatment group,model group and normal group.Using Rene method,7time-periods were adopted.Purified mouse peritoneal macrophages were obtained and detected with flow cytometer.RESULTS:After48-hour treatment,the subgroups of positive CD16/32,positive CD80and neg?ative CD16/32changed significantly.CONCLUSION:Topical application of Dieda paste could regulate the expressions of CD 16/32and CD80to normal levels.

Objective To explore the value of video-assisted thoracoscopy for the diagnosis of indeterminate solitary pulmonary nodules.Methods A total of 42 patients with indeterminate solitary pulmonary nodules(

Objective To explore the nutrition support route of enteral nutrition (EN) and parenteral nutrition(PN) on the cellular immune function of patients after esophagectomy.Methods Sixty-two patients with esophageal surgery were randomly divided into EN group and the PN group,and each was 31 cases.Cellular immune indexes(including T lymphocyte subsets,natural killer cells,B lymphocytes) were measured at the preoperation,the postoperative 1st day and 8th day.The t test was preformed to compare the measurement data of two groups,and repeated measures analysis of variance was used to compare multiple time points of measurement data of two groups.Results The levels of CD3,CD4,CD8,CD4/CDS,NK cells and B cells in EN group at before operation were (60.46 ± 6.41) ％,(39.03 ± 5.64) ％,(25.06 ± 5.39) ％,(1.60 ± 0.24),(13.86 ±4.91)％,(13.14 ± 3.81)％ respectively,and those indices at 8th day after operation were (65.18 ±7.81)％,(40.45 ±6.82)％,(22.81 ±4.47)％,(1.78 ±0.16),(16.97 ±4.85)％,(15.69 ± 3.88)％ respectively,and the differences were statistically significant(P ＜ 0.05).The levels of CD4,CD4/CD8,NK cells and B cells in PN group at before operation were (59.78 ± 5.17) ％,(39.10 ± 5.49) ％,(24.94 ± 3.97) ％,1.57 ±0.20,(14.02 ± 4.47) ％,(12.96 ± 3.39) ％ respectively,significantly different from that of 8th days ((59.78 ±5.17)％,(39.71 ±5.63)％,(24.74 ±3.80),(1.62 ±0.15),(15.35 ±4.31)％,(14.33 ±3.43) ％ respectively),and there were significant difference compared to before operation in terms of CD4,CD4/CD8,NK cells and B cells.At the 8th day after operation,there was significant difference between in EN group and PN group in terms of CD3,CD8,CD4/CD8,NK cells and B cells(P ＜0.05).Conclusion Early EN for patients after esophageal carcinoma operation can significantly improve the cellular immune function of patients.

Objective To investigate the significance of early postoperative enteral nutrition with esophageal cancer in the elderly. Methods A total of 62 cases of esophageal cancer patients aged 70 years and older were randomly divided into the enteral nutrition(EN)group and the parenteral nutrition(PN)group. In preoperative and the first day and eighth day of postoperative serum albumin,prealbumin,peripheral blood lymphocytes,serum immunoglobulin(IgG,IgM,IgA),complement C3 and C4 were measured,and the postoperative infectious complications and cardiovascular complications were observed. Results During the eighth day after operation,the statistic analysis of the data between EN group and PN group showed: tALB = 2. 535,tPA = 2. 880,tLymphocyte =2. 720,tIgA =2. 166,tIgM =2. 065 ,tC3 =4. 103 ,tC4 =2. 478,P ＜0. 05. The result indicated that the EN group was better than the PN group in improving the nutritional status of patients,strengthened the immune system. The complication comparison between the two groups after operation: x2 Infection = 4. 24,x2 Cardiovascular =15.55,P ＜ 0. 05. The result indicated that the EN group had lower incidence of infectious complications and cardiovascular complications than the PN group. Conclusions Early enteral nutrition can significantly improve the nutritional status of elderly patients with esophageal cancer after postoperative,enhance immunity,and reduce the incidence of complications.

Objective To probe into the indication, operative skill of intrapericardial pneumonectomy, the prevention and cure in postoperative complication of intrapericardial pneumonectomy for locally advanced lung canc-er. Methods The clinical data of 43 cases of locally advanced lung cancer undergoing intrapericardial pneumonec-tomy from February 1998 to November 2007 were retrospectively analyzed. Results No surgical death occurred. The main postoperative complication was arrhythmia, acute pulmonary edema and pneumonia, with incidence as 41. 86%. The 1-,3- and 5-year postoperative survival rate was 79.1% ,30.2% and 11.6%. Conclusions The intrap-ericardial pneumonectomy skill not only can improve resection rate, reduce exploration rate, but also offer more oppor-tunity of further therapy in a part of locally advanced lung cancer.

Objective By quantitative tissue velocity i maging(QTVI),to observe longitudinal wall motion characteristics during isovolumic contraction, ejection, isovolumic relaxation, early relaxation, atrial systole phases in regional myocardium of myocardial infarction patients with three diseased blood vessels, and to assess postsystolic shortening in isovolumic relaxation phase and its clinical meanings. Methods Left ventricular regional myocardium of 30 myocardial infarction patients (MI group) and 30 non-cardiac disease subjects (control group) was assessed by QTVI. After dynamic images were collected and information was analyzed off-line, left ventricular myocardial velocity and time velocity integral profiles were acquired along long axis asynchronously in basal and middle segments of different walls, peak velocities (V IC , V S, V IR , V E, V A) during different phases and maximum time velocity integral (TVI max )in regional myocardium was measured. Results Peak velocities(V IC , V S, V IR , V E, V A) in different segments of left ventricular regional myocardium in MI group were lower than those of control group, especially more obviously in infarcted segments. Peak velocity amplitude in ejection declined, but it had positive velocity spike; abnormal positive velocity in isovolumic relaxation phase suggested postsystolic shortening. What's more, time velocity integrals in some segments of MI group had two wave crests, reached maximum in isovolumic relaxation phase, but were still lower than that of control group. Conclusions Systolic and diastolic function in MI patients with three diseased blood vessels are apparently impaired; abnormal movement in isovolumic relaxation phase suggests existence of postsystolic shortening and abnormal wall motion in regional myocardium.V IR is a marker of abnormal regional wall motion and ischemic myocardium.

Objective To measure various parameters with tissue Doppler imaging, quantitatively score left ventricular wall motion, assess longitudinal wall motion in total and regional myocardium of myocardial infarction patients. Methods Tissue tracking was adopted for assessing left ventricular regional myocardium of 30 myocardial infarction patients (MI group) and 30 normal subjects (control group). After collecting dynamic images and analyzing information off-line, maximum displacement along long axis asynchronously in annular, basal, mid and apical segments of different walls were acquired, and left ventricular wall motion score indexes(TT score indexes) were calculated. Results Maximum displacement in different segments of left ventricular regional myocardium was lower in MI group than control group, especially in infarcted segments. TT score indexes were lower in MI group than control group( 6.23? 1.93 vs 9.71? 1.08, P