The mice B lymphocyte hybridoma cells were irradiated by 60Co?-rays with doses of 1, 2, 4, 6. 8Gy. A markedly dose-relation depression in cell survival rate, cell concentration and clone-forming rate was observed. D37 value of clone-forming rate was 8.26 Gy. Dose-relation depression was also observed in total production of monoclonal antibody of clones. But production of monoclonal antibody per clone was raised with radiation doses. These results indicate that ionization radiation depresses the survival activity of hybridoma cells, but stimulates the secretion of monoclonal antibody of survival cells.

OBJECTIVE To explore associated factors of nosocomial infection and provide ways for control nosocomial infection.METHODS The associated factors of nosocomial infection were analyzed by using retrospective analysis in 29 842 cases from Jan 2004 to Dec 2005.RESULTS Among 29 842 cases,1917 cases were infected,the incidence was 6.4%.CONCLUSIONS The associated factors of nosocomial infection are age,the underlying diseases,the decrease in immunological function and the invasive manipulation.

OBJECTIVE To undersand neurosurgical hospital infection characteristics and bacterial resistance,and to provide reference information for the rational use of antibacterial drugs in clinics.METHODS Using the combined methods of initiative monitoring and recalling system to carry on the clinical informations' statistics,the analysis and the judgment for the 880 inpatients in the neurosurgery department in 2005.RESULTS The rate of neurosurgical hospital infection was 16.36%,significantly higher than the hospital average rate(9.18%) at the same period.Neurosurgical hospital infection pathogens were mainly Gram-negative bacteria.Infection sites occurred mainly in urinary tract(44.44%),followed by lower respiratory tract(35.42%).Sixty strains were Escherichia coli(20.2%),34 strains(11.44%) for Enterococcus faecium(D group),25 strains(8.42%) for Acinetobacter baumannii,25 strains(8.42%) for Pseudomonas aeruginosa,and 18 strains(6.06%) for Klebsiella pneumoniae.CONCLUSIONS Systematic monitoring of neurosurgical hospital infection characteristics and dynamics of bacterial resistance has important clinical reference significance to the rational use of antibacterial drugs.

OBJECTIVE To understand the characteristic of hospital infection among tumor patients and give information to reduce the tumor patients with hospital infection.METHODS Using the methods of initiative monitoring and the system review to carry on the statistics,analysis,and evaluation for the total of 2679 tumor patients occurred in Department of Oncology in Shaoxing People′s Hospital in 2005.RESULTS Among them there were 120 cases occurred hospital infection.The rate of hospital infection was 4.47%.The patients who infected one time were 179 cases,two times were 31 cases,and over three times were 10 cases.The main infected sites were respiratory tract including upper respiratory tract(38 cases) and lower respiratory tract(36 cases),followed by alimentary tract(10 cases),blood(9 cases),surgical incision infection(8 cases),urinary tract(6 cases) and the other locations(13 cases).CONCLUSIONS The main prevention and control measures of hospital infection among tumor patients are to improve the body immunity and control endogenous infections.

OBJECTIVE To achieve the best treatment effect by the effective management to control abusing the clinical antibacterial drug. METHODS The management organization and system on antibiotics reasonable application were established.It also formulated an standard to examine the reasonable antibiotics application.It construced the information system of the antibiotics reasonable application management,to carry on the real-time monitoring to the clinical antibiotics application situation. RESULTS The antibiotics utilization ratio was from original 78% lowered to 65% and the antibiotics use hastened more reasonably. CONCLUSIONS The standardized management of antibiotics use is realized and the medical quality is improved.

OBJECTIVE To explore risk factors and preventive measure of wound infection after operations in clean wounds.METHODS The related factors of wound infection were analyzed by using Logistic analysis in 6805 cases from Jun 2004 to Dec 2005.RESULTS Age,duration of operation and diabetes mellitus were the risk factors for wound infection,but the antibiotic application was unrelated to wound infection in clean wounds.CONCLUSIONS The risk factors for wound infection are age,duration of operation and diabetes mellitus.

Objective To explore the related infection factors of the internal jugular vein indwelling central venous catheter , and make prevention countermeasures according to the infection factors of central venous catheter infections .Methods A total of 564 patients admitted in liver and gallbladder surgical ward with external jugular vein indwelling central venous catheter were se‐lected ,extract the relevant hospital infection data in patients by the XingLin hospital infection real‐time monitoring system ,and SPSS15 .0 statistical analysis was conducted .Results The infection rate of 564 cases of patients was 4 .07% .The rate of gram‐neg‐ative bacteria infection was 43 .5% ,the gram positive bacteria infection accounted for 34 .8% ,fungi accounted for 21 .7% ,including multiple drug‐resistant bacteria infection accounted for 52 .1% .Catheter indwelling 14 d or more infection rate was 8 .5% ,14 d fol‐lowing infection rate was 2 .1% ,and infected patients for more advanced cancer and patients with severe acute pancreatitis .Pipe joint respectively with heparin cap and needle positive pressure infusion joint connections ,infection rate was statistically different (P<0 .05) .Conclusion The infection of internal jugular vein indwelling central venous catheter should not be ignored ,and the oc‐currence of catheter‐related infection of patients is closely related to state of an illness ,the time of catheter insertion ,and the joint device and so on .

OBJECTIVE To analyze the current situation of the hospital.and the community-acquired infections due to Staphylococcus aureus(SAU) and provide the reference for clinical reasonable use of drugs.METHODS The clinically isolated S.aureus strains during 2004-2006 were collected,cultured and identified.Their susceptibilily to 16 kinds of antibacterials was detected by K-B or MIC and WHONET5 software was used to analyze the result.RESULTS From 440 S.aureus strains,the meticillin-resisstant S.aureus(MRSA) was 260 accounting for 59.1%;the meticillin-sensitive S.aureus(MSSA) was 180 accounting for 40.9%.The resistant rate of SAU to penicillin G was the highest(85.5%),that to vancomscin was 0.CONCLUSIONS MSSA still keeps fairly good sensibility to most antibacterial medicine,but MRSA shows the multidrug resistance,except to vancomycin.For this studying the resistance mechcnism of Staphylococcus and continuously detecting the emergence of vancomycin-intermediat and vancomycin-resistante S.aureus have a significant clinicil importance.

OBJECTIVE To investigate the distribution and resistance of Pseudomonas aeruginosa isolated during Jan-Dec 2006.METHODS The clinically isolated P.aeruginosa strains were collected cultured and identified by paper diffusing method or trace dilution method(MIC),the results were evaluated according to the relevant documents of NCCLS of USA.RESULTS The in vitro susceptibility test of 244 P.aeruginosa isolates to 16 kinds of antibacterials indicated the resistance rate to SMZ was the highest(98.8%);then to minocycline,tetracycline and ticarcillin/clavulanicacid(70.1%,58.6% and 54.5% respectively).CONCLUSIONS To strengthen the continuous survezillance of drug resistance of P.aeruginosa,to sum up the resistance rules of main pathogens of departments in hospital and to reduce of production of resistant bacterica have the important significance.

OBJECTIVE To understand the antibiotic resistance of Pseudemonas aeruginosa isolated from ICU and give advices to clinicians.METHODS The antibiotic susceptibility of P.aeruginosa isolated from ICU to 12 kinds of antibiotics were tested by disc diffusion method.RESULTS The antibiotic susceptibility of 472 P.aeruginosa strains to imipenem,piperacillin/sulbactam,piperacillin,amikacin,ceftazidime,tobramycin,cefepime,ciprofloxacin,ceftriaxone,gentimicin,aztreonam and cefotaxime were 82.2%,74.94%,69.92%,64.87%,59.95%,52.93%,49.88%,49.65%,44.50%,41.92%,38.17%,and 35.60%,respectively.CONCLUSIONS P.aeruginosa isolated from ICU of our hospital is suscepitable to imipenem,piperacillin/sulbactam,piperacillin,amikacin and the cephalosporins,but show lower susceptibility to other antibiotics.