Thyroid cancer is one of common malignant tumor in neck, which can occur in any age. Females are more likely to have this disease than males. Most patients get a nodule on their thyroid, which typically does not cause any symptoms. Surgical resection is an effective method to cure thyroid cancer. In recent years, more and more evidence support that traditional Chinese medicine plays an important role in the recovery of postoperative thyroid carcinoma.

Objective To summarize the clinical experience of operation treatment of Lisfranc fracture dislocation,and evaluate its clinical effect.Methods Thirty-nine patients with Lisfranc joint injuries (46 sides) were managed with open reduetlon as well as internal fixation with serews and Kirsehnerwlres.Results All the patients were followed up from12.0 to 42.0 months,the average was (21.1 ± 1.8) months.Evaluated the clinical effect according to the American Orthopaedic Foot and Ankle Society (AOFAS) midfoot score standard,there were 29 cases of excellent,8 cases of good,and 3 cases of aceeptable.Conclusion By Kirschner wire combining with screw fixation,open reduction and internal fixation may achieve satisfactory clinical results for the treatment of Lisfranc joint injurie.The operation is simple and fast,small trauma,less complications and can obtain good effects.

OBJECTIVE To quantify the HBV DNA in saliva of chronic hepatitis B(CHB) patients and to study its relation to the periodontal status of the patients. METHODS HBV DNA were isolated from 60 patients with CHB by Trizol-chloroform method.HBV DNA levels were tested by real-time PCR technique and their relationship with plaque index,gingival index and probing depth was analyzed. RESULTS The positive rate of HBV DNA was (56.7%,) and the level of HBV DNA was 4.16+0.57(LogE,copies/ml) in saliva.The detectability of HBV DNA in saliva had no significant correlation with the values of oral clinical parameters. CONCLUSIONS The detectability and level of HBV DNA in saliva have no correlation with the oral hygienic parameters.Other sources of the HBV DNA may exist besides the serum in saliva.

Objective To observe the effect of platelet-rich plasma(PRP)gel on tendon-bone healing following tendon allograft reconstruction of anterior cruciate ligament(ACL).Methods Bilateral ACL reconstructions using Achilles tendon allografts were performed in 24 New Zealand white rabbits matured skeletally.One knee joint was pretreated with the allograft PRP gel(served as experimental group),while the contralateral knee joint was free from treatment with PRP(served as control group).The reconstructions were assessed histologically,immunohistochemically and biomechanically at 2,6 and 12 weeks.Results At 2 and 6 weeks,Burak scores of experimental group were higher than control group.At 12 weeks,the grafts showed a mature zone of fibrocartilage in experimental group but mature scar tissues on the tendon-bone surface.Immunohistochemistry demonstrated early higher expression of VEGF in experimental group than control group and continually higher expression of TGF-β1 in experimental than control group.In contrast,the grafts of the controls group revealed the development of mature scar tissue resembling Sharpey fibers spanning the tendon-bone interface.At 2 and 6 weeks,the biomechanical analysis revealed the limit load of(15.3±2.9)N and(33.2±6.9)N respectively in experimental group,which were significantly higher than(7.9±1.4)N and(23.7±4.9)N in control group (P ＜ 0.05).Conclusion Application of PRP is the potential means to enhance the earlier healing of the allograft tendon-bone.

Objective To observe angiogenic effect of platelet-released growth factors (PRGF) from platelet-rich plasma (PRP) on mierovessel formation at early stage after anterior crueiate ligament (ACL) reconstruction by freeze-dried Achilles tendon. Methods The study involved 14 rabbits, of which 12 rabbits were used as experiment group and the other 2 as control group. In the experiment group, after two sides of rabbit ACL were removed, freeze-dried Achilles tendon treated by PRGF was transplanted into random one side of the knee to substitute the original ACL (PRGF group), while the other side was transplanted with freeze-dried Achilles tendon treated only by normal saline (NS group). Only one side of the knee was removed in the control group. The grafts were observed by HE and immuno- histeehemical staining 2, 4 and 6 weeks after operation. Microvessel density (MVD) was measured by Weidner method. Results Compared with NS group, MVD in PRGF group was significant higher at 2,4 and 6 weeks after operation (P<0.05). MVD of NS group at 2,4 and 6 weeks after operation was 2.52±0.45, 3.41±0.44 and 2.57±0.51 respectively, but that of PRGF group at 2,4 and 6 weeks af- ter operation was 3.56±0.81,4.91±0.46 and 3.01±0.75 respectively (P<0.05). The time of neo- vascular formation and the depth of vascular penetration into the grafts of the PRGF group were superior to those of NS group. Conclusion PRGF can significantly promote microvessel formation at early stage after ACL reconstruction with freeze-dried Achilles tendon.

Objective To analyze the clinical feature of the patients with anti -N -methyl -D -aspartate receptor (NMDAR)encephalitis and feature of brain MRI and electroencephalogram (EEG)in children.Methods We reviewed the clinical manifestations,brain MRI and EEG features of 10 patients who were diagnosed anti -NMDAR encephalitis.Results Of the 10 patients,3 cases were male and 7 cases were female.The age was ranged from 13 months to 14 years (6 years and 11 months on average).No tumor was found in those patients.Main symp-toms included seizure in 9 cases,psychiatric symptoms in 10 cases,consciousness disturbance in 4 cases,involuntary movements in 8 cases,autonomic nerve instability in 5 cases,and sleep disorders in 10 cases.7 patients of MRI exami-nation were normal,2 patients revealed abnormal signal of temporal lobe,frontal,and parietal cortex.One patient revealed brain atrophy.All patients had abnormal EEG with diffusive slow waves,and some with focal spikes or sharp waves,left side abnormal more than right side.Conclusion Anti -NMDAR encephalitis can be found in children, even young boys may be affected.They have a lower incidence of tumors.Its predominant clinical features are psychi-atrics symptoms,seizures,involuntary movements and consciousness alteration.EEG was considered more significant than brain MRI.

Altogether 80 Wistar rats were used for an animal model of cerebral concussion, which were sacrificed on days 1,3,7,14 and 30 after injury and the brain tissue was collected. The pathologic changes of cerebral concussion and apoptosis of nervous cells were studied by means of light microscopy, electron microscopy and in situ terminal end labeling method. The results showed that the clinical situation for cerebral concussion occurred in rats struck by 100g standard weight from 1 meter high. The basic pathologic changes were the cerebral vascular dilatation, congestion, hemorrhage, and edema of cerebral tissue. Nervous cells underaent degeneration, apoptosis and necrosis, and the Nissl bodies obviously decreased, even disappeared. On days 1～3 after injury, dot or piece necrosis was seen in brain tissue, around which the tissue rarefied. Monocytes and foam cells increased, and lots of neurons underwent degeneration, apoptosis and necrosis. The edema of cerebral tissue reached its peak on day 7. Hippocampal pyramidal cells decreased in number and showed the changes of obvious ischemia. On days 14～30, blood vessels also showed dilatation, congestion and hemorrhage, whereas edema alleviated. The neurons in cerebral cortex and hippocampus also showed the changes of chronic ischemia. By in situ terminal end labeling the number of apoptotic neurons increased on day 1, reached its peak on day 3 and still existed on day 30. The results suggested that the main pathologic changes of cerebral concussion were blood circulatory disorder and nervous cell degeneration, apoptosis and necrosis. Apoptosis of nervous cells was one of the main changes in cerebral concussion.

Objective To clone and analyze the MN/CA9 gene sequence in ICR mice and detect the expressions of MN/CA9 gene in various tissues of ICR mice.Methods The fresh tissues of small intesine, uterrus, skin, musle, liver, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach ,urinary bladder from ICR mice were obtained , the total RNA was extracted by GIT method, the 1st strand and 2nd strand of cDNA were synthesized, the EcoRⅠ adapters were lingated,the EcoRⅠ ends were phosphorylated, digested with XhoⅠ ,cDNA was ligated into the ZAP expression vector, packaged, planted, screened.The expressions of MN/CA 9 gene in various tissues in mice were detected by RT-PCR.Results A fragment of human MN/CA9 gene was used as probe, and 1.47?10~3 clones were screened with radioactive isotopic ~ 32 P labeled probe, after hybridizations, one positive signal of cDNA was detected and the complete nucleotide sequence contained 1 671 bp was determined (GenBank:AB086322), The nucleotide similarity between mouse and human cDNA (GenBank:Z54349) was 69.1%.The MN/CA9 gene detected by RT-PCR assay (primer: P521-P1193) strongly expressed in small intesine,uterus, musle, pancreas, heart, lung, thymus, spleen, kidney, ovary, stomach,and urinary bladder,meanwhile did not express in skin and liver. Conclusion The expressions of MN/CA9 gene in some tissues of ICR mice are similar to that of human, it can be used to further functional analysis of MN/CA9 gene.

Adenocarcinoma ; genetics ; metabolism ; pathology ; Biopsy ; CD56 Antigen ; metabolism ; DNA-Binding Proteins ; metabolism ; Gene Deletion ; Humans ; Keratin-7 ; metabolism ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Male ; Middle Aged ; Receptor, Epidermal Growth Factor ; genetics ; metabolism ; Small Cell Lung Carcinoma ; genetics ; metabolism ; pathology ; Synaptophysin ; metabolism ; Transcription Factors

A series of tacrine-methoxybenzene hybrids (5a-5i) were designed, synthesized and evaluated as inhibitors of cholinesterases (ChEs). All the compounds had better ChEs inhibitory activities than tacrine with IC50 values at the nanomolar range. Compound 5h exhibited the strongest inhibition on acetylcholinesterase (AChE) with an IC50 value of 6.74 nmol x L(-1) and compound 5f showed the most potent inhibition on butyrylcholinesterase with IC50 value of 3.83 nmol x L(-1). Kinetic and molecular modeling studies showed that these hybrids targeted both the catalytic active site and the peripheral anionic site of AChE.