1.Identification of the genus and species of the dacryocystitis-causing bacteria by 16S rRNA gene
Na, AN ; Xian-ning, LIU ; Ya-xian, LAN ; Sha, TAO
Chinese Journal of Experimental Ophthalmology 2013;31(9):867-869
Background Dacryocystitis is one of the most common infectious eye diseases.The gold standard for the identification of bacteria causing dacryocystitis is bacterial culture.The combination of regular culture method with molecular biology techeniques will generate more reliable results.However,very few research data are available in ophthalmological studies in this area.Objective This study was to identify the genera and species of the dacryocystitis-causing bacteria by PCR amplification of the 16S rRNA sequences.Methods Ten cases of qualified standardized bacteria samples were taken,and the nucleic acids were released in the heating process of the PCR procedure.The 16S rRNA genes were amplified and sequenced,and the genera and species were identified using BLAST from GenBank,and the results were used to compare with the results from biochemical identification to test the reliability of this method.The cultured bacterial species from the lacrimal sac secretions from 30 cases of dacryocystitis patients were identified with the above method.Results The outcome of the PCR identification for the 10 cases of quality control standard bacterial specimens was consistent with the results from the biochemical identification.The identification of the 30 cases of dacryocystitis through sequencing the 16S rRNA revealed there were 13 cases of Staphylococcus epidermidis infection,2 cases of Staphylococcus warneri infection,1 case of Staphylococcus hominis infection,5 cases of Corynebacterium macginleyi infection,3 cases of Streptococcus pneumonia infection,2 cases of Bacillus cereus infection,1 case of Micrococcus luteus infection,1 case of Moraxella catarrhalis infection,1 case of Moraxella osloensis infection and 1 case of Pseudomonas aeruginosa infection.Conclusions Sequencing the 16S rRNA is an accurate and specific way for the identification of the genera and species of bacteria that cause dacryocystitis in patients.This sequencing method is feasible in monitoring a variety of dacryocystitis-causing pathogens.More information and epidemiological statistics about dacryocystitis can be obtained from 16S rRNA sequencing.
2.The analysis of plasmid-mediated AmpC enzyme genotype and epidemiology of Escherichia coli and Klebsiella pneumoniae
Fu-Ying FENG ; Xiao-Peng LAN ; Xian-Yue YANG ; Ya-Bin ZHANG ; Xin-Lan HU ; Rong-Ying GUO ;
Chinese Journal of Laboratory Medicine 2001;0(03):-
Objective To investigate the prevalence,genotype and epidemiology of plasmid- mediated AmpC enzyme of Escherichia coli and Klebsiella pneumoniae.Methods A total of 67 clinical isolates of nonrepetitive cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae collected by Fuzhou General Hospital and Fujian Provincial Hospital during a period of Sept.2004 to Mar.2005 were detected by three-dimensional extract test for AmpC enzyme,and PCR for AmpC enzyme and other ?-lactamase gene amplification and DNA sequencing were carried out for genotype of ?-lactamase.Plasmid transformation experiment was used to study the transfer of cefoxitin resistance.The homology of the isolates was determined by ERIC-PCR fingerprinting.Results At two hospitals in Fuzhou,the prevalence of plasmid-mediated AmpC enzyme among cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae were 16.7% and 10.5%, 8.0% and 0,respectively.Two isolates of Klebsiella pneumoniae produced DHA-1 plasmid-mediated AmpC enzyme,and 4 isolates of Escherichia cob and one strain of Escherichia coli produced CMY-2 and CMY-22 plasmid-mediated AmpC enzyme respectively.Furthermore,5 strains of Escherichia coli with CMY AmpC enzyme were also found simuhaneously to produce TEM-144,CTX-M-27,CTX-M-14 and TEM-1 ?-lactamase respectively.Three strains of Escherichia coli and one isolate of Klebsiella pneumoniae could transfer cefoxitin resistance to acceptant bacillus.ERIC-PCR fingerprinting reveals 2 strains of Klebsiella pneumoniae came from same clone,but 5 strains of Escherichia coli came from different clones.Conclusions The clinical isolates of Klebsiella pneumoniae producing DHA-1 plasmid-mediated AmpC enzyme and Escherichia coli producing CMY-2,CMY-22 plasmid-mediated AmpC enzyme are found in Fuzhou.CMY-22 AmpC enzyme and TEM-144 ?-lactamase are the first reported in the world,GenBank accession number: DO256079,DO256080
3.The associations between idiosyncratic adverse drug reactions and HLA alleles and their underlying mechanism.
Qing WANG ; Hu MEI ; Ya-Lan ZHANG ; Xian-Chao PAN ; Wen TAN ; Li CHAO
Acta Pharmaceutica Sinica 2013;48(6):799-808
With the advent of Twenty-First century, more and more genome-wide association studies (GWAS) showed that idiosyncratic adverse drug reactions (ADRs) were closely related with human leukocyte antigen (HLA) alleles, such as the associations of abacavir-HLA-B*5701, allopurinol-HLA-B*5801, and carbamazepine-HLA-B*1502, etc. To explore the mechanisms of these idiosyncratic drug reactions, hapten hypothesis, danger signal hypothesis, pharmacological interaction (P-I) concept and autoimmune mechanism are proposed. In this paper, recent GWAS studies on the HLA-mediated adverse drug reactions and underlying mechanism are reviewed in detail.
Alleles
;
Allopurinol
;
adverse effects
;
Anti-HIV Agents
;
adverse effects
;
Anticonvulsants
;
adverse effects
;
Carbamazepine
;
adverse effects
;
Dideoxynucleosides
;
adverse effects
;
Drug Hypersensitivity Syndrome
;
etiology
;
immunology
;
Drug-Related Side Effects and Adverse Reactions
;
genetics
;
immunology
;
Enzyme Inhibitors
;
adverse effects
;
Genome-Wide Association Study
;
HLA Antigens
;
genetics
;
HLA-B Antigens
;
immunology
;
HLA-B15 Antigen
;
immunology
;
Humans
;
Stevens-Johnson Syndrome
;
etiology
;
immunology
4.Current status of social anxiety and depression among primary school students in Urumqi, China.
Su-Lan LIN ; Dan WANG ; Ya-Jing XIAN ; Jing YANG ; Ping-Ping WU
Chinese Journal of Contemporary Pediatrics 2018;20(8):670-674
OBJECTIVETo investigate the current status of social anxiety and depression among the fourth-, fifth-, and sixth-grade students in Urumqi, China, and to provide data support for mental health education for primary school students in the future.
METHODSStratified cluster random sampling was performed to select 919 fourth-, fifth-, and sixth-grade students from four primary schools for the Han and minority ethnic groups in Urumqi as research subjects. Social Anxiety Scale for Children and Children's Depression Inventory were used to evaluate the current status of social anxiety and depression.
RESULTSAmong the 919 fourth-, fifth-, and sixth-grade students, the detection rate of social anxiety was 28.5% (262 students). The fourth-grade students had significantly lower scores on each subscale of social anxiety and total score of social anxiety than the fifth- and sixth-grade students (P<0.05). Girls had significantly higher score on the subscale of fear of negative evaluation and total score of social anxiety than boys (P<0.05). The Uyghur students had significantly higher scores on each subscale of social anxiety and total score of social anxiety than the Han students and the students of other minority ethnic groups (P<0.05). Among these fourth-, fifth-, and sixth-grade students, the detection rate of depression was 11.2% (103 students). The fourth-grade students had significantly lower scores on the subscales of negative self-esteem, ineffectiveness, and interpersonal problems and total score of depression than the fifth- and sixth-grade students (P<0.05). The Uyghur students had significantly higher scores on the subscales of anhedonia and negative mood and total score of depression than the Han students and the students of other minority ethnic groups (P<0.05).
CONCLUSIONSThere are significant differences in the status of social anxiety and depression among the primary school students with different grades, sexes or ethnic groups in Urumqi. The fifth- and sixth-grade students, girls, and Uyghur students tend to have more serious psychological problems, which should be taken seriously by parents, schools, and the society.
5.Similarities and differences on complications after cranioplasty with two-dimensional and three-dimensional digital shaping titanium meshes
Jun-Xian HU ; Yu-Ming YUAN ; De-Ying ZHAO ; Chun-Lin XU ; Ya-Lan WU
Chinese Journal of Tissue Engineering Research 2018;22(18):2826-2830
BACKGROUND: Patients with cranial defects undergoing cranioplasty can develop complications related or unrelated to repair materials. OBJECTIVE: To explore the differences and similarities between the two-dimensional and three-dimensional digital shaping titanium meshes for cranioplasty. METHODS: The clinical data of 221 patients with skull repair were retrospectively analyzed. Two-dimensional digital shaping titanium mesh was used in 61 cases, and three-dimensional digital shaping titanium mesh used in 160 cases. Postoperative complications related (including exposure of titanium mesh and nail and loosening of titanium nail) or unrelated (including refractory subcutaneous effusion, epilepsy, scalp necrosis, scalp infection, intracranial infection and intracranial hematoma) to repair materials were summarized. RESULTS AND CONCLUSION: There were 14 cases of complications (4 related and 10 unrelated) in the two-dimensional digital shaping titanium mesh group, including 2 cases of intractable subcutaneous effusion, 7 cases of epilepsy, 2 cases of titanium mesh and nail exposure, 2 cases of titanium mesh and titanium nail loosening and 1 case of scalp infection. There were 17 cases of complications (0 related and 17 unrelated) in the three-dimensional digital shaping titanium mesh group, including 5 cases of refractory subcutaneous effusion, 9 cases of epilepsy, 1 case of scalp necrosis, 1 case of intracranial infection and 1 case of intracranial hematoma. Significant differences in the complications related to repair materials were found between the two groups (χ2=5.577, P=0.018). Overall findings suggest that the craniotomy with three-dimensional digital shaping titanium mesh can cause fewer material-related complications than that with two-dimensional digital shaping titanium mesh.
6.Current status of social anxiety among primary school students with myopia in Urumqi, China and risk factors for myopia.
Dan WANG ; Jing YANG ; Ya-Jing XIAN ; Ping-Ping WU ; Su-Lan LIN
Chinese Journal of Contemporary Pediatrics 2019;21(2):184-188
OBJECTIVE:
To investigate the prevalence of social anxiety among the fourth-, fifth- and sixth-grade primary school students with myopia in Urumqi, China and the risk factors for social anxiety.
METHODS:
Stratified cluster random sampling was used to select 552 fourth-, fifth- and sixth-grade primary school students with myopia from four primary schools in Urumqi. A self-designed questionnaire, a social anxiety scale for children and a self-esteem scale were used to investigate the general demographic data and the current status of social anxiety and self-esteem. A multivariate logistic regression analysis was used to investigate the risk factors for social anxiety in primary school students with myopia.
RESULTS:
Of the 552 children, 173 (31.3%) were found to have social anxiety. The multivariate logistic regression analysis showed that a higher grade, female sex, autocratic family, high myopia, low self-esteem and wearing glasses for more than 2 years were risk factors for social anxiety (P<0.05).
CONCLUSIONS
There is a serious problem of social anxiety among the fourth-, fifth- and sixth-grade primary school students with myopia in Urumqi. The development of social anxiety is associated with age, sex, degree of myopia, time of wearing glasses, parental education style and self-esteem level.
Anxiety
;
China
;
Female
;
Humans
;
Myopia
;
Risk Factors
;
Students
;
Surveys and Questionnaires
7.Current status of parental feeding behaviors in Urumqi, China, and its association with body mass index of children.
Su-Lan LIN ; Jing YANG ; Dan WANG ; Ping-Ping WU ; Ya-Jing XIAN
Chinese Journal of Contemporary Pediatrics 2018;20(12):1044-1049
OBJECTIVE:
To investigate the association between parental feeding behaviors and body mass index (BMI) of children, and to provide a scientific basis for the prevention and intervention of overweight and obesity in children.
METHODS:
Stratified random cluster sampling was used to select 976 children from 7 kindergartens in Xinshi District of Urumqi, and a questionnaire survey and physical measurement were performed to obtain related data.
RESULTS:
A total of 976 questionnaires were distributed and 924 (94.7%) usable questionnaires were collected. The overall detection rates of underweight, overweight, and obesity in children were 3.1% (29 children), 9.2% (85 children), and 6.7% (62 children), respectively. The highest level of parental feeding behavior was monitoring, followed by restriction and pressure to eat. The parents of the Han children had significantly higher levels of restriction and pressure to eat than those of the Uygur children (P<0.01). As for parental feeding behaviors, restriction to eat was positively correlated with BMI of the Han and Uygur boys (P<0.01); pressure to eat was negatively correlated with BMI of the Han boys and girls (P<0.01) and was positively correlated with BMI of the Uygur boys and girls (P<0.01); monitoring was negatively correlated with BMI of the Han and Uygur boys and girls (P<0.05). Among the parents of the Han and Uygur boys, the parents of the overweight/obese children had significantly higher scores of restriction to eat than those of the children with normal weight (P<0.05); among the parents of the Uygur boys and girls, the parents of the overweight/obese children had significantly higher scores of pressure to eat than those of the children with normal weight (P<0.01); among the parents of the Uygur and Han boys and girls, the parents of the overweight/obese children had significantly lower scores of monitoring than those of the children with normal weight (P<0.01).
CONCLUSIONS
The parental feeding behavior is good in Urumqi, and the parental feeding behavior of the Uygur children is a little better than that of the Han children. Parental feeding behavior is closely correlated with BMI of children, and such correlation differs across ethnic groups and sexes. A high level of monitoring and low levels of restriction and pressure to eat may help to prevent and control the development of overweight and obesity in children.
Body Mass Index
;
Body Weight
;
Child
;
China
;
Feeding Behavior
;
Female
;
Humans
;
Male
;
Overweight
;
Parents
;
Surveys and Questionnaires
8.Changes of mossy fiber sprouting and P-glycoprotein expression in the hippocampus of rat models of amygdala-kindling epilepsy
Shi-Xian MAO ; Ya-Mei FENG ; Lan CHU ; Yun-Li YU ; Qi LIU
Chinese Journal of Neuromedicine 2013;12(7):657-660
Objective To explore the relationship between plasticity ofhippocampus neuronal morphology and pathogenesis of epilepsy by observing the changes of mossy fiber sprouting (MFS) and P-glycoprotein (P-gp) expression in the hippoeampus of rat models of amygdala-kindling epilepsy.Methods Ninety male Wistar rats were randomly divided into epilepsy model group (n=40),drug treatment group (n=40) and sham-operated group (n=10).Models of chronic epilepsy were established by stimulating the amygdale; rats in the drug treatment group were perfused antiepileptic drug levetiracetam into stomach [100 mg/(kg·d),twice daily].At different observation time points (1,2,4 and 8 weeks after the treatment),Timm staining was employed to observe the changes of MFS; immunohistochemical method was used to detect the dynamic changes of P-gp.Results (1) After the success of model making,abnormal MFS levels in the hippocampal CA3 transparent layer were noted; lowest MFS scores were noted in S1 subgroup,which showed no significant difference as compared with those in sham-operated group (P>0.05); the MFS scores in S2,S4 and S8 subgroups increased gradually with a peak level at 8 weeks after inducement,which showed significant difference as compared with those in sham-operated group (P<0.05).No significant differences on MFS grading scores were noted in the drug treatment group between each two time points (P>0.05).(2) The P-gp expression in the epilepsy model group showed significant difference at different time points (P<0.05); highest expression level was noted at one week after the surgery,and returned to normal at four weeks.However,the P-gp expression showed no significant difference in the drug treatment group between each two time points (P>0.05),except for 1 week after treatment.Conclusion MFS is the important mechanism of chronic epilepsy,and P-gp is the product of epilepsy,which is the main reason of epilepsy drug resistance.
9.Enhancement of tropane alkaloids production in transgenic hair roots of Atropa belladonna by overexpressing endogenous genes AbPMT and AbH6H.
Shi-Ping LONG ; Yan LU ; Ya-Xiong WANG ; Chun-Xian YANG ; Xiao-Zhong LAN ; Zhi-Hua LIAO
Acta Pharmaceutica Sinica 2013;48(2):243-249
Atropa belladonna L. is the officially medicinal plant species and the main commercial source of scopolamine and hyoscyamine in China. In this study, we reported the simultaneous overexpression of two functional genes involved in biosynthesis of scopolamine, which respectively encoded the upstream key enzyme putrescine N-methyltransferase (PMT; EC 2.1.1.53) and the downstream key enzyme hyoscyamine 6beta-hydroxylase (H6H; EC 1.14.11.11) in transgenic hair root cultures of Atropa belladonna L. HPLC results suggested that four transgenic hair root lines produced higher content of scopolamine at different levels compared with nontransgenic hair root cultures. And scopolamine content increased to 8.2 fold in transgenic line PH2 compared with that of control line; and the other four transgenic lines showed an increase of scopolamine compared with the control. Two of the transgenic hair root lines produced higher levels of tropane alkaloids, and the content increased to 2.7 fold in transgenic line PH2 compared with the control. The gene expression profile indicated that both PMT and H6H expressed at a different levels in different transgenic hair root lines, which would be helpful for biosynthesis of scopolamine. Our studies suggested that overexpression of A. belladonna endogenous genes PMT and H6H could enhance tropane alkaloid biosynthesis.
Atropa belladonna
;
enzymology
;
genetics
;
Gene Expression Regulation, Enzymologic
;
Gene Expression Regulation, Plant
;
Hyoscyamine
;
metabolism
;
Methyltransferases
;
genetics
;
metabolism
;
Mixed Function Oxygenases
;
genetics
;
metabolism
;
Plant Roots
;
enzymology
;
genetics
;
Plants, Genetically Modified
;
enzymology
;
genetics
;
Plants, Medicinal
;
enzymology
;
genetics
;
Scopolamine Hydrobromide
;
metabolism
;
Synthetic Biology
;
Tropanes
;
metabolism
10.Mechanism of cleft palate in mice induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin.
Ya-Lan PU ; Li-Ling LIU ; Li-Qiang GAN ; Xiao-Meng HE ; Yue-Xian FU
Chinese Journal of Plastic Surgery 2011;27(6):448-453
OBJECTIVETo explore the mechanism of cleft palate in mice induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD).
METHODSOn gestation day 10 (GD 10), 12 pregnant mice were randomly divided into two groups as the treated group and the control group with 6 mice in each group. The mice in the treated group received intragastric administration with 64 microg TCDD/kg, while the mice in the control group received equivalent corn oil. The embryos were examined under stereomicroscope to detect the incidence of cleft palate on GD 18.5. Another 18 pregnant mice were randomly divided into two groups (treated group and control group) on GD 10 with 9 pregnant mice in each group. Then each group was divided into 3 subgroups: GD 13.5, GD 14.5 and GD 15.5, with 3 pregnant mice in each subgroup. The palatal shelves were dissected from the embryos for RNA and DNA extraction on GD 13.5, GD 14.5 and GD 15.5. At last the expression of Smad 2-4 and Smad 7 mRNA was investigated by RT-PCR, and the TGF-beta3 promoter methylamine levels were investigated by methylation specific PCR (MSP).
RESULTSThe cleft palate mice model was established successfully by exposing pregnant C57BL/6J mice to TCDD. Total frequency of clefts was 100% in TCDD group, and the frequency of clefts was 0 in the control group. The relative expression of Smad 2 mRNA was 0.263 +/- 0.088, 0.296 +/- 0.016 and 0.159 +/- 0.027 in TCDD group, 0.180 +/- 0.042, 0.282 +/- 0.029 and 0.165 +/- 0.018 in control group. The relative expression of Smad 3 mRNA was 0.453 +/- 0.153, 0.551 +/- 0.160 and 0.328 +/- 0.049 in TCDD group, 0.375 +/- 0.126, 0.510 +/- 0.145 and 0.259 +/- 0.035 in control group. The relative expression of Smad 4 mRNA was 0.675 +/- 0.174, 0.577 +/- 0.070 and 0.396 +/- 0.066 in TCDD group, 0.557 +/- 0.138, 0.587 +/- 0.080 and 0.441 +/- 0.054 in control group. The relative expression of Smad 7 mRNA was 0.283 +/- 0.050, 0.320 +/- 0.068 and 0.169 +/- 0.045 in TCDD group, 0.207 +/- 0.043, 0.288 +/- 0.051 and 0.155 +/- 0.040 in control group. There was no significant difference between the TCDD treated mice and the control (P > 0.05). The TGF-beta3 promoters were at the un-methylation state both in the TCDD treated and control group.
CONCLUSIONIt suggests that TCDD could induce a stable formation of cleft palate, but it is not through the TGF-beta/Smad signaling nor through the modification of TGF-beta3 promoter methylation.
Animals ; Cleft Palate ; chemically induced ; DNA Methylation ; Female ; Mice ; Mice, Inbred C57BL ; Polychlorinated Dibenzodioxins ; toxicity ; Pregnancy ; Promoter Regions, Genetic ; Signal Transduction ; Smad Proteins ; metabolism ; Teratogens ; toxicity ; Transforming Growth Factor beta3 ; metabolism