Objective To determine if there is an early developmental resistance to seizure-induced hi ppocampal damage. Methods Five daily pentylenetetrazol-indu ced convulsions in immature rats beginning at postnatal day P10,P60 groups.In b oth groups, the latency of seizure, the latency of Ⅳ/Ⅴ grade, the lasting time of seizure and mortality of rats after seizure were used to measure sensitivity of seizure or the resistance to brain damage. Conventional histopathological me thod was utilized to observe morpbological changes and cell counting of dentate granule cells, CA 3,CA 1 and hilar neurnns. Timm histochemical technique was a dopted to study mossy fiber sprou- ting.Results 1.In the both groups(P10,P60),there were significant differences in the latency of seizure (1.07?0.55 vs 8.27?1.48 P

Objective To investigate molecular biological basis associated with inflammatory and immune response in Qi deficency and blood stasis syndrome. Methods Twelve rats were randomly divided into model group and control group. Rat Genome 230 2.0 Array Gene Chip was used for examination of gene expression profiles in rats with Qi deficiency and blood stasis syndrome and normal rats. Differental expression genes associated with inflammatory and immune mechanism were analyzed. Results Compared with the control group, there were different expression genes in model group, the up-regulated were 831 genes, known functional genes 563, and down-regulated 782, known functional genes 544. The expression level increased in 50 genes and decreased in 17 genes associated with inflammatory and immune response. Conclusion Differental expression genes associated with inflammatory and immune response revealed that there was close correlation between Qi deficency and blood stasis syndrome and abnormal inflammatory and immune response.

Objective To establish a satisfactory method of isolating,culturing and determining gut neural crest stem cells,which may provide theoretical basis for clinical application.Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium.The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs.Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.Results Neurospheres were generated in the simplified serum-free medium.The staining results showed that enteric neurospheres were GNCSCs and could differentiate into neurons,glial cells and smooth muscle cells by serum-induction.Conclusion GNCSCs have the capacity of self-renewal and proliferation,and give rise to neurons,glial cells and myofibroblasts.

Objective To observe the therapeutic effects of combination of TCM with western medicine for depressive neurosis.Methods The therapeutic group included 30 cases,patients were treated by combination of TCM with western medicine.The control group included 30 eases,patients were treated only with western medicine.Four weeks was a course of treatment.Results The efficacy of the therapeutic group was 96.7% ,the efficacy of the control group was 66.7% ,there is a significant difference between the two groups(P <0.01).Conclusion The combination of TCM with western medicine for depressive neurosis is effective with short course of treatment.

Objective To assess global and regional cardiac twist function in hypertrophic cardiomyopathy(HCM) patients by velocity vector imaging(VVl).Methods Thirty HCM patients and 33 normal subjects were enrolled into this study.Two dimensional echocardiographic images of parasternal left ventricular basal level,middle and apical level short axis plane,apical four-and two-chamber plane were obtained.Maximal rotation degree,peak rotation rate,circumferential strain (CS) and strain rate (CSR),radial strain(RS) and strain rate(RSR) in systole were measured using VVI offline software.In addition,diastolic peak un-rotation rate,untwisting rate(unTwR) were measured.Each parameters was analyzed both in subendocardial and subepicardial layer.Results There was no significant difference in left ventricular ejection fraction between two groups.EDV,ESV,SV were significantly decreased (P＜0.01) in HCM group.Left ventricular twist(LVtw),CS of subendocardial layer were higher in HCM group than control group(P＜0.05),while the peak rotation rate,un-rotation rate,CS,CSR were lower of subepicardial layer (P＜0.05).The RSR of each short axis level were significantly decreased in HCM group (P＜0.01).The diastolic unTwR was decreased in HCM group (P＜0.05).For further analysis of the two subgroups in HCM,more regional torsion deterioration occurred in patients with left ventricular outflow tract obstruction than those without obstruction.Conclusions The global cardiac twist angle of HCM was higher than normal person,but the circumferential deformation ability of regional myocardium was impaired,left ventricular outflow tract obstruction may aggravate the regional myocardial dysfunction.

Objective To study the exposure dose-effectiveness of ? irradiation on killing the plasmodium in the mice RBC,for the further exploration on the method that could kill the plasmodium in RBC without affecting the activity and function of normal RBC. Methods After infection with Plamodium yoelii (P.y),blood was collected from mice and exposed to ? irradiation (radiated group). An unirradiated group served as control. In the irradiated group,P.y infected blood was divided into three aliquots,each aliquot was irradiated one time by ? radiation using Gammacell 1000 Elite blood radiation apparatus. The dosage of each aliquot was 25,35 and 45Gy. After irradiation,the blood samples were stored at 4℃. Then mice were inoculated with these irradiated blood stored for 1,3 or 5 days after irradiation,or with unirradiated blood. Two days later,the blood samples were taken from inoculated mice and were examined under microscope and plasmodium infection rates were calculated. Results The mice in the control group had parasitemia much earlier than those in irradiated group (1—2 days),and the plasmodium infection rate in the control group was significantly higher than that in the irradiated group(3.7% vs 0.07%). With increasing dosage of irradiation,the survived plasmodium in blood decreased,and survival of mice increased(8—12 days). After 45 Gy irradiation and 5 day storage at 4℃,there were no plasmodium found in the red blood cell of inoculated mice. In the control group,blood testing result was positive,and all the mice died.Conclusion Plasmodium in mice RBC can be killed effectively when blood is exposed to 45Gy irradiation and stored at 4℃ for 5 days.

Aim To explore the role of prenatal exposure to lipopolysaccharides(LPS)on aortic morphology in the 6-week offspring rats.Methods Twelve pregnant rats were randomly divided into three groups: control group,LPS group,L+P(pyrrolidinedithiocarbamate,PDTC)group.The rats were intraperitoneally administered vehicle,LPS(0.79 mg·kg-1),or LPS plus PDTC(100 mg·kg-1).LPS was given on the 8th,10th and 12th day,where as vehicle and PDTC were given daily from the 8th to the 14th day during gestation.Offspring body weight was measured at 6-week,and histopathological alteration of the thoracic aorta was observed by transmission electron microscopy,the thoracic aorta mRNA and protein expression of connexin(Cx)molecules including Cx37,Cx40,Cx43 and Cx45 in offspring rats were detected by real time PCR,Western blot and confocal laser-scanning microscope.Results Body weight at 6-week offspring rats was significantly higher in LPS group than in control group whatever male or female(P<0.01);and that in L+P group was significantly lower than in LPS group in male rats(P<0.05),but not in female rats.The LPS group thoracic aortas exhibited lesions,including impaired endothelial cells,and the vascular smooth muscle cells were arranged disorder and migration and proliferation;the number of gap junction was increased and longer than control group;the pathological changes were improved to some extent in L+P group.Cx43 mRNA and protein expression in LPS group was obviously lower than in control group(P<0.05),which could be partly reversed in L+P group(P<0.05).There was no significant difference in Cx37,Cx40 or Cx45 mRNA or protein expression among each group.The results of protein expression with confocal laser-scanning microscope was the same as those of Western blot.Conclusion Maternal LPS exposure during pregnancy leads to vascular changes in 6-week offspring rats,which might last until neonatal stage,and is closely correlated to hypertension in adult offsprings.

OBJECTIVE: To simulate the distribution state of lutein molecules in modified starch by dissipative particle dynamics method. METHODS: Materials Studio 4.0 Software was used with Visualizer Module to build 3D models of lutein and modified starch. The Discover and Amorphous Cell modules were used to calculate the solubility parameter. Dissipative particle dynamics module was used to simulate lutein distribution in the microcapsules. SEM was used to investigate the structure of the microcapsules. RESULTS: Dissipative particle dynamics simulations showed the aggregation morphology of modified starch inclusion of lutein molecules and the bead movement ability. Dissipative particle dynamics numerical simulation was verified by in vitro experiments, and the results were satisfactory. The feeding ratio of modified starch and lutein was 100;40. The microcapsules production rate was 47.63%, and the microcapsulation efficiency was 85.79%. The particle size of the microcapsules was 70 μm. CONCLUSION: Dissipative particle Dynamics simulation can show the distribution of drugs in microcapsules very well and can be used to predict the feeding ratio.

Objective To assess the effectiveness of different concentration of iodized salt for preventing iodine deficiency disorders. Methods Using the principle and method of systematic review, we searched Cochrane Library(from 1994 to Mar. 2007), Medline(from 1966 to Mar. 2007), BA(from 1969 to Mar. 2007), PubMed(from 1950 to Mar. 2007), OC1D(from 1950 to Mar. 2007), ISI Web of Knowledge(from 1966 to Mar. 2007), Vip (from 1989 to Mar. 2007), Wanfang(from 1997 to Mar. 2007), CBMDisc(from 1978 to Mar. 2007) and CNKI(1994 to Mar. 2007) and hand searched 6 relevant Chinese journals, including Chinese Journal of Preventive Medicine, Chinese Journal of Endemiology, Chinese Journal of Epidemiology, Chinese Journal of Control of Endemic Disease, Endemic Diseases Bulletin and Modern Preventive Medicine. We screened the for eligible studies according to the inclusion and exclusion criteria to be rigorously evealuatecl descriptly and qualitatively. Results Thirteen studies were included, of which, the first six were intervention trials with comparison, including two community intervention trials, which classified all objects into different groups, using iodized salt at different concentration as intervention, four were RCTs with different intervention methods, compare the iodized salt with other intervention measures. Seven were cross-sectional studies, which analyzed the iodine nutrition of people after the concentration of iodized salt was lowered down. Because of different interventions in control groups and different outcome measures, it was difficult to perform recta-analysis, a descriptive analysis of the results was presented. Most studies showed that urinary iodine level decreased as the concentration of iodized salt went down gradually. When the concentration of iodized salt went down to the best level recommended, goiter rate decreased obviously. Conclusions Iodized salt was considered as the hest method of iodine supply to prevent iodine deficiency disorders. But effectiveness of preventing iodine deficiency disorders with various concentration of iodized salt is different. To lower the concentration of iodized salt properly can not only prevent iodine deficiency disorders but also reduce the side effect of excess iodine intake to the minimum. And it can also save a lot of iodine resource. Well-designed community-based intervention trials with large sample size are needed to confirm the effect of different concentration of iodized salt on preventing iodine deficiency disorder.